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Citations for Thermo Fisher :
9001 - 9050 of 10000+ citations for Glutathione Fluorescent Detection Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... 0.5-1 μg of total RNA was reverse transcribed using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). SYBR Green PCR master mix (Kapa Biosystems ...
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bioRxiv - Cell Biology 2023Quote: ... 1 µg of cDNA was synthesized using a high-capacity cDNA reverse transcription kit (Applied Biosystems, Waltham, MA, USA). PCR experiments were performed using 20 ng of cDNA per reaction ...
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bioRxiv - Neuroscience 2024Quote: ... Both fractions were then used to measure the amyloid beta 1-42 (Aβ1-42) using a specific ELISA kit (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was synthesized from 1 μg of total RNA using the High-Capacity cDNA Reverse Transcription kit (Applied Biosystems). The mixture was incubated at 25°C for 10 min ...
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bioRxiv - Cancer Biology 2023Quote: ... washed in PBS and incubated with 1:100 LIVE/DEAD Fixable Near-IR Dead Cell Stain Kit (Life Technologies), according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Total RNA (1 µg) was converted to cDNA using the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher #4368814). The mRNA levels were detected using amfisure qGreen Q-PCR Master Mix (GenDEPOT #Q5602) ...
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bioRxiv - Plant Biology 2023Quote: ... and cDNA was syntethized from 1 µg of RNA using the High Capacity cDNA Reverse Transcription kit (Applied Biosystems). For the real-time RT-PCR analysis ...
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bioRxiv - Immunology 2023Quote: ... YT and KHYG-1 cells was performed with the Pierce Cell Surface Protein Isolation kit (Thermo Scientific, cat#89881) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... 1 µg of total DNase I-treated RNA was reverse transcribed using the Superscript first-strand synthesis kit (Invitrogen). The cDNA was diluted in DNase-RNase free water ...
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bioRxiv - Physiology 2023Quote: ... 1 µg of total RNA was transcribed into cDNA by using a SuperScript™ III Reverse Transcriptase kit (Invitrogen) and random hexamer primers ...
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bioRxiv - Bioengineering 2023Quote: ... cDNA was synthesized from 1 µg of RNA by following the protocol of cDNA conversion kit (4368814, Applied Biosystems). Real time PCR was performed using SYBR™ Green Master Mix (A25742 ...
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bioRxiv - Cell Biology 2024Quote: ... 1 μg RNA from HUVECs was subjected to reverse transcription with the High-Capacity RNA-cDNA kit (Applied Biosystems). RT-qPCR was performed from the resulting cDNA in a CFX 384 thermocycler (Bio-Rad ...
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bioRxiv - Genetics 2024Quote: ... PCR amplicons were cleaned up using QIAGEN’s QIAquick PCR Purification Kit (QIAquick) and separated by electrophoresis on 1% E-Gel EX Agarose Gels (Invitrogen). For sequencing ...
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bioRxiv - Bioengineering 2024Quote: ... JC-1 assay kit (Cat. No. M34152) and Dulbecco’s modified eagle medium (DMEM) (Cat. No. 11965084) were purchased from ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: 1 μg of RNA was utilized for reverse transcription using a high capacity cDNA reverse transcription kit (Applied Biosystems). Quantitative RT-PCR was performed using a Bio-Rad CFX96 ThermoCycler (Bio-Rad Laboratories ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 1 μg total RNA was reverse transcribed with RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific, Waltham, USA) using an oligo (dT ...
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bioRxiv - Neuroscience 2024Quote: ... RNA samples (1 μg) were reversely transcribed (High-Capacity cDNA Reverse Transcription Kit; Thermo Fisher Scientific, Waltham, MA, USA) and real-time PCR analysis was performed using 1μl of cDNA in a reaction of 10 μl ...
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bioRxiv - Immunology 2024Quote: ... and viral GP gene amplified by RT-PCR (Verso 1-Step RT-PCR kit; Thermo Fisher Scientific AB-1455), cloned and sequenced as previously described 48.
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bioRxiv - Biophysics 2021Quote: ... The recombinant constructs of heavy and light chain were transfected at 1:1 ratio into Expi293F™ cells using the ExpiFectamine™ 293 Transfection Kit (Gibco™, Thermo Fisher Scientific, Waltham, MA, USA). 4-5 days after transfection the antibodies were purified from the supernatant by protein A affinity resin (ProSep®-vA ultra ...
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bioRxiv - Plant Biology 2020Quote: ... fructose and sucrose were analyzed in the same samples used for total WSC analysis using high performance anion exchange chromatography with pulsed amperometric detection HPAEC-PAD (Dionex ICS-5000; Thermo Fisher Scientific, Sunnyvale, USA). Separations were performed at 30 °C and the flow rate was 0.5 mL/min ...
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bioRxiv - Genomics 2020Quote: ... The qPCRs were run of both technical and biological replicates (n=3) using an ABI7500 sequence detection system (Applied Biosystems by Life Technologies, Darmstadt, Germany). Fold expression changes were determined using a standard 2−ΔΔCT method that compares CT (cycle threshold ...
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bioRxiv - Cancer Biology 2020Quote: ... Taqman assays were used in combination with Taqman Universal Master Mix and run on a 7900 HT sequence detection system (Applied Biosystems, Foster City, CA). Cycling conditions were as follows:
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bioRxiv - Physiology 2021Quote: ... mRNA levels were quantified using gene-specific primer pairs by real-time RT-PCR on an ABI PRISM 7900HT Sequence Detection System (Thermo Fisher Scientific, MA, US) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: The total DNA extract was used in a qPCR reaction using the Biorad CFX Connect™ real-time PCR detection system with PowerUp™ SYBR® Green Master Mix (Applied Biosystems) in 10µl reactions according to the manufacturer’s directions to determine mtDNA copy number ...
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bioRxiv - Cell Biology 2022Quote: ... Chemiluminescence was detected with the ECL Prime Western Blotting Detection Reagent (Cytiva, GERPN2232) and SuperSignal™ West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific, 34094). The bands were visualized using an Odyssey infrared imaging system (LI-COR).
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bioRxiv - Cancer Biology 2022Quote: ... and gene specific primers were used for PCR amplification and detection on a 7500 FAST Real-Time PCR System (Applied Biosystems, Thermo Fisher Scientific). Relative mRNA levels were determined by comparing the PCR cycle thresholds between cDNA of a specific gene and beta actin for mouse or histones for human (ΔCt).
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bioRxiv - Cell Biology 2019Quote: Real-Time PCR analysis was performed on an ABI Prism 7500 detection system (Applied Biosystem, CA, USA) using Power SYBR Green PCR Master Mix (Applied Biosystems, Thermo Fisher Scientific). Relative changes in gene expression were calculated using the 2-ΔΔCt method normalized against the housekeeping gene 18s ...
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bioRxiv - Physiology 2021Quote: ... membranes were washed again for three times with TBST for five minutes followed by detection with PierceTM ECL Plus Western Blotting Substrate (Thermo Fisher Scientific, Waltham, MA) and imaging by Azure c600 ...
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bioRxiv - Cell Biology 2019Quote: ... Quantitative real-time PCR reactions were performed with SYBR green PCR reagents using the ABI Prism 7300 sequence detection system (Applied Biosystems-Life Technologies, Waltham, MA). Relative gene expression levels were calculated using the 2 delta Ct method (Livak & Schmittgen ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Membranes were washed (6×5 min) with TBS-T and proteins were visualized by quimioluminiscence (GE Healthcare Amersham™ ECL™ Western Blotting Detection Reagents, Fisher Scientific) in a Fujifilm LAS-3000 imager ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantitative RT-PCR was performed with Fast SYBR green master mix on the ABI StepOne Plus Sequence Detection System (Applied Biosystems, Foster City, CA) using the primers listed in supplemental table 2 ...
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bioRxiv - Molecular Biology 2024Quote: ... for 30 s were the cycling conditions used for RT-qPCR in a SDS-7500 Sequence Detection System device (Applied Biosystems, Waltham, MA, USA). Ta and Tfa conditions specific for each primer pair are listed in Table 1 ...
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bioRxiv - Bioengineering 2024Quote: ... qRT-PCR was performed by the Mount Sinai qPCR CoRE using an ABI PRISM 7900HT sequence detection system with robotic arms (Applied Biosystems, Foster City, CA). Data was analyzed using the 2−ΔΔCt method (74) ...
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bioRxiv - Plant Biology 2024Quote: ... Quantitative RT-PCR was performed with CFX Connect Real-Time PCR Detection System using Maxima SYBR Green/Fluorescein qPCR Master Mix (Thermo Scientific, Cat No. K0242). The relative expression level of the genes was normalized against the reference gene UBC21 by ΔCT Method ...
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bioRxiv - Genetics 2024Quote: ... resuspended in hexanes and analyzed by gas chromatography with flame ionization detection (GC-FIDs; two Thermo Scientific TRACE 1300 GC-FID systems were used) as described in Bourgault et al ...
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bioRxiv - Molecular Biology 2024Quote: ... Readout detection probes were hybridized at a final concentration of 0.1 µM together with 2 µg/ml DAPI (Thermo Fisher Scientific, catalog number D21490) in a hybridization buffer of 20% formamide in 2×SSC at 37°C for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... following kit instructions (BCA Protein Assay kit, ThermoFisher #23225) and 20µg loaded into each well of a NuPAGE 4-12% Bis-Tris Gel (Invitrogen ...
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bioRxiv - Synthetic Biology 2021Quote: ... All luciferase assays were conducted in LumiNunc 96-well white plates (Thermo Scientific, #136101). NanoLuc luciferase was normalized by constitutively expressed firefly luciferase.
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bioRxiv - Biophysics 2019Quote: ... Reactions were performed in triplicate in a 384 well optical plate (Thermo Scientific Nunc). The plate was detected by a Varioskan® Flash microplate reader (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: ... Plates were run on a QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher), and data were analyzed using the comparative CT method to generate expression fold-change values ...
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bioRxiv - Cell Biology 2020Quote: ... The culture plate was then put into a microplate reader (Thermo Scientific, Multiskan GO) for inspection of the absorbance value at 450 nm and this optical density (OD ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293T cells were transiently transfected in 6-well plates with Lipofectamine 2000 (Life Technologies), as per the manufacturer’s instructions ...
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SIRT1 ameliorates premature senescence-induced defenestration in hepatic sinusoidal endothelial cellbioRxiv - Cell Biology 2020Quote: ... Primary rat HSECs were cultured in plates with medium comprising 80% MCDB131 (Gibco, 10372019) and 20% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2020Quote: ... 10,000 TZM-bl cells per well were seeded in 96-well plates (Nunc-Greiner). The following day cells were infected with an equal volume of culture supernatant for 4 hr ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were seeded in transparent tissue-culture 96-well plates (Sarstedt, Thermo Fisher Scientific) and allowed to adhere overnight ...
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bioRxiv - Immunology 2021Quote: Macrophages generated as above were harvested from plates using Trypsin-EDTA (Thermo fisher scientific). The indicated target cells were labeled with Celltrace violet (Thermo fisher scientific ...
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bioRxiv - Immunology 2021Quote: ... All qPCRs were performed in MicroAmp Fast Optical 96 WellReaction Plates (Thermo Fisher Scientific) using AppliedBiosystems 7500 Fast Real-Time PCR System with 7500Software V2.0.6 (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed three times with PBS-Tween 20 (Fisher Scientific, Cat. #BP337-500), then blocked with 5% w/v non-fat dry milk (Nestle Carnation ...
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bioRxiv - Developmental Biology 2021Quote: Cells were detached from cell culture plates by incubation with 0.25% trypsin-EDTA (Invitrogen) plus 2% chick serum (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... Plates were washed and free binding sites were then blocked with Superblock (Thermofisher Scientific) at room temperature for 30 min and then incubated with serial dilutions (200- to 12800-fold for serum ...