Labshake search
Citations for Thermo Fisher :
8951 - 9000 of 10000+ citations for Glutathione Fluorescent Detection Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Physiology 2020Quote: ... and cDNA synthesis was performed with 1 μg of total RNA following the manufacturer’s instructions of the 3’ RACE kit (Invitrogen). Polymerase chain reaction (PCR ...
-
bioRxiv - Cancer Biology 2021Quote: ... 1 µg of total RNA was used for cDNA synthesis using the Maxima First Strand cDNA kit (ThermoFisher Scientific). qPCR assays were conducted using Taq platinum (Invitrogen ...
-
bioRxiv - Microbiology 2021Quote: ... Real-time RT-PCR was performed using the Power SYBR Green RNA-to-CT 1-Step Kit (Applied Biosystems) and an AriaMx Real-Time PCR system (Agilent).
-
bioRxiv - Molecular Biology 2021Quote: ... Quantitative RT-PCR analysis was performed using the Power SYBR Green RNA-to-Ct 1-Step kit (Applied Biosystems) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... the qPCR technique was carried out using the Verse 1-step RT-qPCR SYBR Green ROX kit (Thermo Scientific) and the protocols recommended by the manufacturer of the LightCycler® 96 termal cycler (Roche) ...
-
bioRxiv - Immunology 2021Quote: ... 1 μg of total RNA was used for reverse transcription via the SuperScript VILO cDNA Synthesis Kit (Invitrogen, #11754050) per the manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... PCR products were visualized on a 1% agarose gel and purified using the PureLink Quick Gel Extraction Kit (Invitrogen). PCR amplicons were fragmented and barcoded using NexteraXT DNA Library Prep Kit ...
-
bioRxiv - Immunology 2021Quote: ... CCR10 (248918) from R&D, CD11a (M17/4) from eBioscience, FcεRI (MAR-1), c-Kit (CD117, 2B8) from Invitrogen and Mouse BD Fc Block™ ...
-
bioRxiv - Microbiology 2020Quote: ... 10 μL of purified proteins (∼1 mg/ml) were digested using Smart Digest Soluble Trypsin Kit (Thermo Fisher Scientific) according to the manufacturer’s guidelines ...
-
bioRxiv - Genomics 2021Quote: ... kit DAPI stain was replaced by a 1/2000 working stock in PBS (Invitrogen D1306 in DMF, 5mg/ml), and slides were mounted in Prolong diamond (Invitrogen #P36970) ...
-
bioRxiv - Cell Biology 2021Quote: cDNA were prepared from 1 ug of total RNA with a Maxima First Strand cDNA Synthesis kit (Thermofisher, #1641) including an additional step of DNAse treatment before reverse transcription ...
-
bioRxiv - Genetics 2020Quote: The total RNA from THP-1 cells was extracted with the mirVana™ miRNA isolation kit (AMBION, catalogue AM1561) and subsequently converted to cDNA using the RevertAid H Minus First Strand cDNA Synthesis Kit (Thermo scientific ...
-
bioRxiv - Biophysics 2020Quote: ... S cerevisiae cells (around 1.5·107 cells.mL-1) stained with LIVE/DEAD™ Yeast Viability Kit (L7009, ThermoFisher Scientific) were mixed with alginate solution in presence of Rhodamine B (0.1 mM ...
-
bioRxiv - Cancer Biology 2022Quote: ... RT was performed with 1 ug of total RNA using cDNA Reverse Transcription kit according to the manufacturer’s protocols (Invitrogen), followed by qPCR using SYBR Green Master Mix (BioRad ...
-
bioRxiv - Cancer Biology 2022Quote: ... and 1 μg of total RNA was used for cDNA synthesis by using the SuperScript VILO kit (Life Technologies). qPCR was performed using inventoried TaqMan assays for respective target genes and housekeeping control genes (18S ...
-
bioRxiv - Genetics 2022Quote: ... We reverse-transcribed 1 μg of RNA with the High-Capacity RNA-to-cDNA Kit (#4387406, Thermo Fisher Scientific), and performed quantitative qPCR with PowerUp SYBR Green Master Mix (#A25742 ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... and quality was assessed on a 1% agarose gel before quantification using the Qubit dsDNA HS Assay Kit (Invitrogen). Library preparation and subsequent RRBS sequencing were carried out at SciLife (Uppsala University ...
-
bioRxiv - Microbiology 2022Quote: ... 25 uL reaction mixtures were prepared using the TaqMan® RNA-to-Ct™ 1-Step Kit (Thermo Fisher) per manufacturer instructions ...
-
bioRxiv - Microbiology 2022Quote: ... Real-time RT-PCR was performed with the Power SYBR Green RNA-to-CT 1-Step Kit (Applied Biosystems) using a AriaMx Real-Time PCR system (Agilent) ...
-
bioRxiv - Microbiology 2023Quote: ... 1 μg of RNA was reverse transcribed to cDNA with the High-capacity cDNA Reverse Transcription Kit (ThermoFisher, 4368814) following the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2023Quote: ... Fura-2 fluorescence was calibrated with defined Ca2+ buffers generated with the Ca2+ Calibration Kit #1 (Invitrogen, Waltham, USA).
-
bioRxiv - Molecular Biology 2022Quote: ... first-strand cDNA synthesis was performed using 1 µg total RNA with MMLV Reverse Transcription Kit (Applied Biosystems, Brazil) and poly-T primer ...
-
bioRxiv - Microbiology 2022Quote: ... using qScript 1-Step SYBR Green qPCR kit (Quantabio) on a ViiA 7 Real-Time PCR System (Applied Biosystems); 16S rRNA was used for normalization ...
-
bioRxiv - Neuroscience 2022Quote: ... Retrotranscribed cDNA was obtained from 1 μg of total RNA using the SuperScript VILO Retrotranscription kit from Life Technologies according to the manufacturer’s instructions.
-
bioRxiv - Microbiology 2024Quote: ... Standards for viral copy number were created by cloning PCR product using custom BdDV-1 primers into the pCR 2.1-TOPO vector using the TOPO TA cloning kit (Invitrogen). Transformed Escherichia coli colonies with successful insert were plasmid extracted using the Zyppy Plasmid Miniprep kit (Zymo Research) ...
-
bioRxiv - Microbiology 2024Quote: Extracted RNA was subjected to RT-qPCR using the TaqMan RNA-to-CT 1-Step Kit (ThermoFisher, Waltham, MA) in a 20μL reaction with 0.5μM of primers and 0.1μM of probe labeled with FAM and ZEN/Iowa Black quenchers (IDT ...
-
bioRxiv - Bioengineering 2024Quote: ... cDNA was synthesized from 1 µg of RNA by following the protocol of cDNA conversion kit (4368814, Applied Biosystems). Real time PCR was performed using SYBR™ Green Master Mix (A25742 ...
-
bioRxiv - Microbiology 2024Quote: ... Reverse-transcriptase quantitative PCR was performed with Power SYBR Green RNA to CT 1-Step Kit (Applied Biosystems #4389986). cysG was detected with JCP241/242 and was used as the housekeeping for normalization ...
-
bioRxiv - Microbiology 2023Quote: ... cDNA synthesis and qPCR were achieved with a Power Sybr Green RNA-to-Ct 1-Step Kit (Applied Biosystems) in 96 MicroAmp Fast Reaction Tubes using an Applied Biosystems StepOne Plus real-time PCR instrument ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... and 1 µg RNA was reverse-transcribed using the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific, Germany). Gene expression levels were analyzed using predesigned QuantiTect primer assays (Hs_GAPDH_1_SG ...
-
bioRxiv - Neuroscience 2024Quote: 0.5-1 ug of RNA was reverse transcribed to cDNA using the High-Capacity cDNA Reverse Transcription Kit (Invitrogen). Following this ...
-
bioRxiv - Pathology 2024Quote: ... and cDNA was synthesized from 1 µg mRNA using a high-capacity cDNA reverse transcription kit (Applied Biosystems, #4368813). mRNA expression levels were measured with a 7500 Real-time PCR system (Applied Biosystems ...
-
bioRxiv - Developmental Biology 2024Quote: ... We generated cDNA from 1 µg of RNA using the SuperScript IV First Stand Synthesis kit (Thermo Fisher Scientific) and analyzed by RT-qPCR using Quant Studio 12K Flex Real Time PCR system version 1.1.2 (Applied Biosystems by Life Technologies ...
-
bioRxiv - Genetics 2023Quote: ... 1 μg of purified total RNA was reverse transcribed using the high-capacity cDNA reverse transcription kit (Applied Biosystems). For specific miRNA quantification ...
-
bioRxiv - Microbiology 2023Quote: ... Real-time RT-PCR was performed using the Power SYBR Green RNA-to-CT 1-Step Kit (ThermoFisher Scientific) on the AriaMx Real-Time PCR system (Agilent ...
-
bioRxiv - Molecular Biology 2023Quote: ... Dead cells were excluded using the Live/Dead Violet Dead cell stain kit (dilution: 1:750; Thermo Fisher Scientific). Cells were washed with 2% FBS and phosphate-buffered saline (PBS ...
-
bioRxiv - Biophysics 2023Quote: ... per sample (37 °C, 1 hr) and cleaned up using the ChargeSwitch PCR Clean-Up Kit (Thermo Scientific CS12000). Successful immobilization of the BRD9 protein was checked by resuspending the beads in 133.3 μL of NuPAGE LDS Sample Buffer (1× ...
-
bioRxiv - Biochemistry 2023Quote: ... per sample (37 °C, 1 hr) and cleaned up using the ChargeSwitch PCR Clean-Up Kit (Thermo Scientific CS12000) (Supplementary Fig ...
-
bioRxiv - Biophysics 2023Quote: ... Standard calcium solutions were prepared in triplicate using the Calcium Calibration Buffer Kit #1 (Molecular Probes Cat. No. C3008MP), following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... The concentration of cDNA was measured by Qubit™ 1× dsDNA High Sensitivity (HS) Assay Kit (Invitrogen, Cat# Q33230). Tagmentation and NGS library was carried out by using TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme ...
-
bioRxiv - Neuroscience 2023Quote: ... Relative gene expression was determined using the TaqMan® RNA-to-CT™ 1-Step Kit (Thermo Fisher Scientific). Reactions were developed in a LightCycler® 96 (Roche) ...
-
bioRxiv - Plant Biology 2023Quote: ... 801–1(10 KU)] kit and the qPCR using the SYBR™ Fast Green Master mix (Thermo Fisher, 4385612).
-
bioRxiv - Physiology 2023Quote: ... Total RNA (1 µg in 20µl) was reverse transcribed using reverse transcription (RT) kits (Applied Biosystems; Foster City, CA) following the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2023Quote: ... Real-time RT-PCR was performed with the Power SYBR Green RNA-to-CT 1-Step Kit (Applied Biosystems) using a AriaMx Real-Time PCR system (Agilent).
-
bioRxiv - Immunology 2023Quote: ... Viable cells were identified using a LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (Thermo Fisher Scientific, 1:200) incubated with conjugated antibodies ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... 1 µg of RNA was used for cDNA synthesis using High-Capacity RNA-to-cDNA™ Kit (Thermo Fisher) that utilizes both random octamers ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... incubated with Alexa Fluor Tyramide 488 (1:100, Alexa Fluor 488 Tyramide SuperBoost Kit, goat anti-rabbit IgG, Invitrogen, Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2023Quote: Spinal cord single cell suspensions were stained with LIVE/DEAD Fixable Stain Kits (1:1000, L10119 or L34959, Invitrogen) to exclude dead cells ...
-
bioRxiv - Neuroscience 2023Quote: ... the single cell suspensions were stained with LIVE/DEAD Fixable Yellow Dead Cell Stain kit (1:1000, L34959, Invitrogen), and surface antibodies ...
-
bioRxiv - Cancer Biology 2023Quote: ... Total RNA (0.5-1 μg) was reverse-transcribed using a High-Capacity cDNA Reverse Transcription kit (Thermo Fisher Scientific). Real-time PCR was performed using 100 ng of cDNA and ABI StepOnePlus (Thermo Fisher) ...