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Citations for Thermo Fisher :
851 - 900 of 1533 citations for Measles Virus Nucleoprotein HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... The viral DNA samples were prepared for quantification by treating the virus with DNaseI (Invitrogen) and Proteinase K ...
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bioRxiv - Microbiology 2020Quote: The nasal swab was initially stored in virus transport medium [Hank’s Balanced Salt Solution (Gibco) containing 2% FCS (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2022Quote: ... qPCRs were set up using TaqMan™ Fast Virus 1-Step Master Mix (Thermo Fisher) and run on an ABI 7500 Fast Real-time PCR System (Applied Biosystems/Thermo Fisher) ...
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bioRxiv - Microbiology 2022Quote: ... the virus inoculum was previously activated with trypsin (200 μg/ml, Gibco Cat. 27250-18) for 1 h at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... The Virus was washed and eluted in Hank’s balanced salt solution (Gibco™, Dublin, Ireland) containing 0.014% Tween-20 (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: Purified virus stocks were diluted in lithium dodecyl sulfate (LDS) sample buffer (Thermo Fisher Scientific) and separated by SDS-PAGE (NuPAGE Novex 10% Bis-Tris gel ...
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bioRxiv - Neuroscience 2022Quote: ... and reverse transcription was performed using Moloney murine leukemia virus reverse transcriptase (Thermo Fisher Scientific). Real-time polymerase chain reaction (PCR ...
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bioRxiv - Synthetic Biology 2023Quote: ... and the precipitated virus pellet was resuspended with 1.1 mL of Opti-MEM (Gibco #31985062) by pipetting and vortexing until fully dissolved ...
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bioRxiv - Microbiology 2023Quote: ... RT-PCR was performed with TaqMan Fast Virus 1-Step Master Mix (Invitrogen, cat. 4444434) in QuantStudio 7 Flex Real-Time PCR System (Thermo Fisher) ...
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bioRxiv - Immunology 2023Quote: ... vRNA was reverse transcribed using the TaqMan Fast Virus 1-Step qRT-PCR kit (Invitrogen) and quantified on a LightCycler 480 instrument (Roche ...
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bioRxiv - Microbiology 2023Quote: ... Virus samples at variable dilutions (1:10 to 1:40,000) were mixed with FluoSphere (Invitrogen) beads (170nm ...
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bioRxiv - Molecular Biology 2023Quote: ... Virus pellet and cell RNA was isolated using TRIzol according to the manufacturer’s protocol (Ambion) and DNase treated ...
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bioRxiv - Microbiology 2023Quote: ... Virus-depleted and concentrated cell SN (in PBS) was added to anti-PA (Thermo Fisher) coated beads and incubated overnight ...
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bioRxiv - Biochemistry 2024Quote: ... Virus generation was performed using the Bac-to-Bac™ expression system (Thermo Fisher Scientific) in Sf9 insect cells ...
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bioRxiv - Microbiology 2024Quote: ... The virus genome was further amplified by PCR (Phusion High-Fidelity DNA Polymerase, ThermoFisher Scientific) with 10 μL cDNA in each 50 μL reaction and MBTuni-12/13 (5′-ACGCGTGATCAGTAGAAACAAGG ...
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bioRxiv - Cell Biology 2020Quote: ... Human PAR4 containing an N-terminal V5-epitope were stably expressed using the HEK293 Flp-In T-Rex cells according to the manufacturer’s protocol (Invitrogen). The concentration of tetracycline was titrated (700 ng/mL ...
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bioRxiv - Molecular Biology 2021Quote: For siRNA transfection experiments: HEK293 cells were grown in 6-well plates and RNAimax transfection reagent (Invitrogen #13778-150) was used following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293 cells were transfected with either NSUN6 wild-type and the miCLIP-mutant construct using Lipofectamine 2000 (Life Technologies) and harvested 24 hours later.
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bioRxiv - Molecular Biology 2020Quote: ... GFP-N was immunoprecipitated from two independent HEK293 cell pellets using 5μg of anti-GFP antibody (Life Technologies G10362). A total of 2% input material was obtained for size-matched control libraries (SMI ...
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bioRxiv - Molecular Biology 2020Quote: HEK293 cells (Flp-In 293 T-REx cell lines) were obtained from Life Technologies (Invitrogen catalogue number R780-07). Cell cultures were maintained in Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Molecular Biology 2019Quote: ... The knottin Fc fusion proteins were expressed in human embryonic kidney (HEK293) cells following the manufacturer’s protocols in the FreeStyle MAX 293 Expression System (Invitrogen). Secreted knottin-Fc fusion proteins were purified using Protein A Sepharose (Sigma ...
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bioRxiv - Genetics 2019Quote: HEK293 cells (CRL-1573, ATCC, VA, USA) were cultured in high-glucose DMEM (Cat. No. 11965092, Life Technologies, USA) supplemented with 10% FBS at 37 and 5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... Human parental HEK293 cells (ATCC, CRL-1573) were cultured in DMEM supplemented with 10% FBS and antibiotics/antimycotics (Gibco).
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bioRxiv - Biophysics 2021Quote: ... The HEK293 cells were transfected with 100 ng/well of pGL3-Fluc plasmid using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol at around 75-90% confluency in a 96 well plate ...
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bioRxiv - Biochemistry 2020Quote: ... Digested peptides from HEK293 and HeLa cells were respectively injected into LTQ-Orbitrap Velos and Q Exactive (Thermo Fisher) onto a 75 μm i.d ...
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bioRxiv - Microbiology 2020Quote: The full length CPSF6 protein was expressed in a suspension-adapted HEK293 cell line (Expi 293F, Thermo Fisher Scientific) by transfection of expression plasmid using ExpiFectamine 293 (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: Human embryonic kidney (HEK293) cells were grown in Dulbecco’s Modified Eagle Medium (DMEM) with 4.5 g/L Glucose and GlutaMAX™ (Gibco) at 37°C and 5% CO2 ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were transfected with GFP constructs (250 ng/well) for 24 (HEK293) or 48 (SH-SY5Y) hours using Lipofectamine 3000 (0.75 µL/well; Invitrogen) as per the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... HCT116 cells, HEK293 or MEF cells were cultured in 10% FBS (Hyclone, SH3007103) in high glucose DMEM (Gibco 11965118) with 1% penicillin/streptomycin at 37°C ...
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bioRxiv - Immunology 2021Quote: HEK293 freestyle cells were transfected with this expression construct in combination with pBu-BirA using 293Fectin (Thermo fisher scientific) transfection reagent at 1μg DNA/10^6 cells ...
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bioRxiv - Neuroscience 2022Quote: ... which were used to directly transfect HEK293-Kir2.1 cells with the linear product using lipofectamine 3000 (Thermo Fisher Scientific). For patch clamp characterization in HEK293A cells ...
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bioRxiv - Developmental Biology 2022Quote: ... HEK293E or HEK293-6E cells were maintained in suspension cultures in Freestyle 293 Expression Media (Gibco, Waltham, MA, USA) supplemented with heat-inactivated fetal calf serum (1% ...
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bioRxiv - Molecular Biology 2019Quote: ... cell or nuclear lysates (400 ug) prepared from HEK293 or HDFs to Dynabeads Protein A (10001D, Thermo Fisher Scientific) that were prebound with 2–3μg of indicated antibody and incubated overnight at 4°C ...
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bioRxiv - Neuroscience 2019Quote: Cells (primary neurons or HEK293 cells) were transfected with 1.6 µg/mL of a pIRES2-EGFP plasmid vector (Invitrogen) harbouring the full-length cDNA coding for the human muscle 6-phosphofructo-1-kinase muscle isoform (PFK1-M)3 (accession number ...
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bioRxiv - Immunology 2019Quote: ... we performed immunoprecipitation of CT-DNA stimulated HEK293 cells using the antibody to IRF3 pS386 crosslinked to Dynabeads (ThermoFisher) overnight at 4 C ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK293 and Huh-7 cells were transfected with plasmid DNAs by standard calcium phosphate precipitation and Lipofectamine 3000 (Invitrogen), respectively.
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bioRxiv - Biochemistry 2021Quote: ... The construct with a N-terminal 8xHis-TwinStrep-GFP-tag were expressed in GripTite HEK293 MSR cells (Thermo Fisher) in black 96 well plates with transparent bottoms ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK 293T and Flp-In HEK293 T-REx were maintained in DMEM-GlutaMAX at 4.5 g*ml-1 D-glucose (Gibco) supplied with sodium-pyruvate ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting vector was transiently transfected into HEK293 cells using the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific # A14525). After enhancement ...
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bioRxiv - Neuroscience 2020Quote: ... were subcloned into the polylinker of the pcDNA3.1 vector (Invitrogene, USA) by standard techniques for transiently transfecting HEK293-F cells (ThermoFisher). Cells were transiently transfected with the plasmids containing the desired GABAAR subunit cDNAs (α ...
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bioRxiv - Biochemistry 2022Quote: ... Untransfected Flp-In T-REx HEK293 cells were cultured under selection with 4 µg/mL blasticidin (Gibco, ThermoFisher Scientific) and 50 µg/mL zeocin (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... Untransfected Flp-In T-REx HEK293 cells were cultured under selection with 4 µg/mL blasticidin (Gibco, ThermoFisher Scientific) and 50 µg/mL zeocin (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... HEK293 cell lines were transfected with pOG44 and pcDNA5/FRT/TO plasmids harboring respective FLAG constructs using Lipofectamine3000 (Invitrogen) as transfection reagent according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: Human embryonic kidney line 293 (HEK293) wildtype and HTT-null cells were grown in Dulbecco’s modified Eagle’s medium (Thermofisher #41965) supplemented with 10% heat-inactivated fetal bovine serum (ThermoFisher #10500064) ...
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bioRxiv - Biochemistry 2023Quote: HEK293S GnTI- cells were grown to approximately 90 % confluency on 24-well tissue culture plates (cat# 11391704, Fisher Scientific) before the medium was exchanged for serum reduced DMEM and the transfection mixture was added ...
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bioRxiv - Physiology 2023Quote: ... IP3R-null HEK293 (HEK-3KO) cells (Alzayady et al., 2016) were maintained in DMEM/F-12 with GlutaMAX (ThermoFisher) supplemented with 10% ...
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bioRxiv - Molecular Biology 2023Quote: NIH3T3 derived Bmal1-luc31 and HEK293/T17 cells were cultured with Dulbecco’s Modified Eagle Medium (DMEM) (Gibco: Cat #11965118) supplemented with 10 % fetal bovine serum (Corning ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... were generated by integrase-mediated homologous recombination of the CFTR sequence into the T-REx HEK293 background (ThermoFisher Scientific). This expression system allows the tetracycline-inducible expression of genes of interest from one defined insertion site ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Parental T-REx-HEK293 cells were cultivated in growth medium: Dulbecco’s modified Eagle’s medium (DMEM) + GlutaMAX-I (#31966, Life Technologies), 10% Dialyzed FBS (Gibco ...
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bioRxiv - Developmental Biology 2023Quote: ... All cell lines were maintained in HEK293 culture medium – Dulbecco’s Modified Eagle Medium (DMEM, high glucose, pyruvate; Gibco, #41966029) with 10% foetal calf serum (FCS ...