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Citations for Thermo Fisher :
751 - 800 of 1533 citations for Measles Virus Nucleoprotein HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... medium containing virus isolates was incubated in Dulbecco’s Modified Eagle Medium (DMEM; Gibco, 11995073) with 1:10000 Vybrant (V22887 ...
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bioRxiv - Immunology 2023Quote: ... Concentrated virus was then incubated using 1:2000 DiD-Cell labelling solution (ThermoFisher V22887) for 20 mins at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... Reverse transcription was performed using Moloney murine leukemia virus reverse transcriptase (Thermo Fisher Scientific) and real-time PCR was performed using TaqMan assays (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Cellular RNA was mixed with TaqMan Fast Virus 1-step Master Mix (Applied Biosystems), 10 mM forward and reverse primers and 2 mM probe ...
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bioRxiv - Biochemistry 2022Quote: HIV-1 virus-like particles (VLPs) were produced by Lipofectamine 3000 (Thermo Fisher Scientific) transfection of 293FT cells (purchased from Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... Strep-tag (NBP2-41073; Novusbio) or influenza virus hemagglutinin tag (2-2.2.14; Thermo Scientific). After washing with PBS with 1% BSA ...
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bioRxiv - Developmental Biology 2023Quote: ... Yamanaka reprogramming factors were induced to iPSCs via Sendai virus using CytoTune2.0 (ThermoFisher, A16517). To establish nGFP+ iPSCs ...
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bioRxiv - Genetics 2023Quote: ... Virus particles were subsequently washed multiple times with PBS (pH 7.4, Thermo Fisher Scientific) and physical titers were measured using a Qubit 3.0 fluorometer and the Qubit dsDNA HS assay kit (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... Virus pellets and cell lysates were dissolved in radioimmunoprecipitation assay (RIPA) buffer (Thermo Scientific) containing protease inhibitors ...
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bioRxiv - Microbiology 2023Quote: ... containing 5 µl of template and TaqMan Fast Virus 1-step mastermix (Applied Biosystems). Primer sequences and concentrations and thermal cycling conditions for SARS-CoV-2 nucleocapsid 1 gene were as previously described (13) ...
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bioRxiv - Immunology 2023Quote: ... PCR reactions were conducted with TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems), forward primer in the 5’ leader region and gene-specific probes and reverse primers as follows ...
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bioRxiv - Developmental Biology 2023Quote: IPS cells were derived from skin fibroblasts using Cytotune Sendai virus kit (Thermofisher, France) and cultured on MEF in KO- DMEM supplemented with KO-SR (Thermofisher ...
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bioRxiv - Neuroscience 2023Quote: ... reverse transcription was performed using Moloney murine leukemia virus reverse transcriptase (Thermo Fisher Scientific). Real-time PCR was performed using TaqMan assays (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... RNA was tested with TaqMan™ Fast Virus One-Step Master Mix (Applied Biosystems) using QuantStudio 6 or 3 Flex Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2023Quote: ... Abelson virus-transformed pre-B cells were maintained in DMEM (Thermo Fisher, 11960-077) supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... along with psPAX2 and pMD2.G packaging and envelope virus using lipofecctamine 3000 (Invitrogen) in serum-free Opti-MEM media (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: ... PCR reactions were conducted with TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems), forward primer in the 5’ leader region and N gene-specific probe and reverse primer as previously described47:
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bioRxiv - Cell Biology 2024Quote: Elephas maximus endothelial and epithelial cells were transduced with Sendai virus (Thermofisher Scientific, A16517) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 µg of total RNA from HEK293 cells or tissue samples was treated with DNAseI Free kit (Thermofisher) followed by cDNA generation using the High capacity cDNA kit (Thermofisher) ...
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bioRxiv - Biochemistry 2020Quote: Dvl2-GFP/dsRed-Axin1 HEK293 cells were seeded at ∼60% confluence in Lab-Tek chamber slides (Nunc 177380) that were pre-coated with 0.1 mg/mL poly-D-lysine (Sigma P6407) ...
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bioRxiv - Biophysics 2019Quote: ... The protein was expressed in HEK293S GnTI− cells (ATCC CRL-3022) grown in Expi293 Expression Media (Life Technologies) to densities 2.5 × 106 cells ml−1 ...
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bioRxiv - Biochemistry 2020Quote: The CR3 headpiece stably transfected HEK293S cells were kept as adhesion cell culture growing in Dulbecco’s Modification of Eagle’s Medium (DMEM) GlutaMAX (Gibco) supplemented with 10 % (v/v ...
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bioRxiv - Neuroscience 2020Quote: HEK293 cells were grown in DMEM with 10% fetal bovine serum (FBS) and 1:100 penicillin-streptomycin (ThermoFisher). In 24-well plates ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Mutations introduced into the δ-subunit were established as stable HEK293 Flp-InTM cell lines (Invitrogen, Paisley, UK), generating a stable cell line for each mutant ...
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bioRxiv - Neuroscience 2020Quote: HEK293 and SH-SY5Y cells (ATCC) were cultured in phenol red-free Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 2 mM L-Glutamine (Invitrogen) ...
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bioRxiv - Biochemistry 2020Quote: HEK293 or HeLa cells were co-transfected with pBABED and pX335 plasmids using Lipofectamine 2000 (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Human Embryonic Kidney 293 (HEK293) cells (ATCC CRL-1573) were cultured in complete growth Dulbecco’s modified Eagle’s medium (DMEM, Gibco), supplemented with 10% fetal bovine serum (UCSF Cell Culture Facility) ...
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bioRxiv - Microbiology 2021Quote: HeLa cells (ATCC CCL-2) and HEK293 cells (ATCC CRL-1573) were maintained in DMEM high glucose (Gibco) containing 10% heat-inactivated fetal bovine serum (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... The recombinant constructs were transfected into HEK293 cells either individually or together using Lipofectamine® 3000 reagent (Invitrogen) following the instructions provided ...
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bioRxiv - Systems Biology 2022Quote: ... HEK293 cells were prepared as 2 × 105 cells/mL in DMEM (Dulbecco s Modified Eagle Media, Life Technologies) + 10% FBS (Foetal Bovine Serum ...
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bioRxiv - Biophysics 2022Quote: ... The Fab version of the antibody fusion (Fab-ShK) was expressed in freestyle HEK293 (293F) cells (Thermo Scientific) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... Human embryonic kidney cells (HEK293) were cultured in culture flask (150 cm2) to 80 % confluence in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco) supplemented with 50 units/ml penicillin G (Gibco) ...
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bioRxiv - Biochemistry 2020Quote: ... HELA cells (DSMZ ACC-57) and HEK293 cells (DSMZ ACC-305) were cultured in DMEM (Thermo Fisher Scientific) supplemented with 10% FCS ...
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bioRxiv - Molecular Biology 2020Quote: ... and renilla luciferase plasmid (1 ng) were transfected into HEK293 cells or HCT116 cells by lipofectamine2000 (Invitrogen, 11668030) following manufacturer’s manual ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... HEK293-T cells were seeded into 10 cm plates with DMEM containing 0.5 mg/mL geneticin G418 (Thermofisher). Once the cells reached approximately 80% confluence ...
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bioRxiv - Microbiology 2022Quote: ... HEK293 FcγRII cells (a gift from Dr. Craig Roy) were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco) supplemented with 10% heat-inactivated fetal bovine serum (HIFBS ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293 Flp-In T-Rex cells were co-transfected with pOG44 Flp-Recombinase Expression Vector (Invitrogen™, V600520), and pcDNA5 FRT TO plasmids expressing appropriate miniTurbo gene fusions ...
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bioRxiv - Neuroscience 2019Quote: HEK293 and SH-SY5Y cells (ATCC) were cultured in phenol red-free Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 2 mM L-Glutamine (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: HEK293 cells were transfected with EBV-BAC DNA using Lipofectamine 2000 reagent (Thermo Fisher Scientific, Waltham, MA, USA) followed by hygromycin selection ...
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bioRxiv - Cell Biology 2020Quote: ... siRNA knockdown of AC1 and AC9 expression in HEK293 cells was carried out using Lipofectamine RNAiMAX (Life Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2021Quote: Two HEK293 cell lines stably expressing mouse STAC3 were generated using the Flp-In T-Rex system (Invitrogen). Host cells ...
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bioRxiv - Biochemistry 2021Quote: ... The N-terminal 8xHis-TwinStrep-GFP-tagged NKCC1 constructs were expressed in GripTite HEK293 MSR cells (Thermo Fisher) in 24 well plates ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 Flp-In™ T-REx™ cell lines were generated according to the manufacturer’s instructions (ThermoFisher Scientific). BirA-containing plasmids (0.4 µg) ...
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bioRxiv - Cell Biology 2022Quote: ... Calcium influx was visualized in N2a cells and HEK293 cells using Fluo-4 AM dye (Fisher Scientific, F14201) or the GCaMP6s HEK293 cell line described above ...
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bioRxiv - Genomics 2022Quote: Adherent HEK293 cell lines were grown in 10 mLs of DMEM (ATCC 302002) containing 10% FBS (Gibco 16000044) and 1% Pen/Strep (Gibco 15070063) ...
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bioRxiv - Microbiology 2022Quote: ... as well as the HEK293 cell line were grown in Dulbecco’s Modified Eagle’s Medium F-12 50/50 (DMEM/F-12, GIBCO) supplemented with 10% FBS at 37°C with 5% CO2 in a humidified chamber ...
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bioRxiv - Molecular Biology 2023Quote: ... Lentivirus production was performed according to the manufacturer’s recommendations in HEK293 E cells using Lipofectamine 3000 (ThermoFisher Scientific). Virus-containing supernatant was collected after 24 and 48 h and combined ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Stable HEK293 cell lines were achieved by antibiotic selection with G-418 (1mg/ml, Gibco-BRL, Paisley, UK) added to the medium 3 days after transfection ...
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bioRxiv - Immunology 2023Quote: HEK293 cells were transfected with pcTBK1-His or pcGSK3B-Flag plasmid using Lipofectamine 2000 (Invitrogen, Waltham, MA, US) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... which then were co-transfected into HEK293 6E cells for expression together following standard protocols for 293Fectin (Invitrogen). The cell cultures were harvested at 5 d post-transfection ...