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Citations for Thermo Fisher :
851 - 900 of 10000+ citations for 5 OXO 5 6 7 8 TETRAHYDRO NAPHTHALENE 1 CARBOXYLIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: HEK:BFP cells were the kind gift of Chris Richardson.8 Cells were maintained at 37°C and 5% CO2 in DMEM media plus GlutaMax (ThermoFisher Scientific) supplemented with 10% (v/v ...
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bioRxiv - Genetics 2019Quote: Xenograft mouse model of human lung cancer tumors were implanted under the left upper limb in the abdomens of 6- to 8-week old male NCG mice by subcutaneous injection of A549 (5×106 cells in 200 µL DPBS (Gibco, C14190500BT)) or H2228 cells (2× 106 cells in 200 µL DPBS) ...
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bioRxiv - Microbiology 2021Quote: ... ExpiCHO cells were cultured in a shaker incubator at 33 °C at 115 rpm with 8% CO2 according to the Max Titer protocol with an additional feed on day 5 (Thermo Fisher). Culture supernatants were harvested 13 to 14 days after transfection ...
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bioRxiv - Microbiology 2021Quote: ... and 2×105 cells were seeded into 24-well culture plates or 5×104 cells into Lab-Tek II 8-well chamber slides (Nunc/Thermo Fisher). To account for donor variations ...
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bioRxiv - Immunology 2021Quote: ... ExpiCHO cells were cultured in a shaker incubator at 33 °C at 115 rpm with 8% CO2 according to the Max Titer protocol with an additional feed on day 5 (Thermo Fisher). Culture supernatants were harvested 13-14 days after transfection ...
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bioRxiv - Physiology 2020Quote: All 7 gills from each individual shrimp (5 ≤ N ≤ 8) were dissected under a magnifying lens with micro-scissors and homogenized in TRIzol reagent (Life Technologies, Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were maintained at 37°C with 5% CO2 with daily replacement of Essential 8 media system (Thermofisher, Canada. Catalog#A1517001). Cells were passaged as aggregates every 3-4 days until they reach 60-70% confluency with 0.5mM EDTA diluted in PBS (Thermofisher ...
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bioRxiv - Microbiology 2022Quote: ... HCT-8 cells were cultured in 25 cm flasks with 5 ml of complete media (RPMI-1640 media [Gibco/Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Human nasal fluid samples were sonicated on ice (5 seconds at amplitude of 8 um; Fisher Scientific Model 705 Sonic Dismembrator) to create homogenous samples ...
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bioRxiv - Developmental Biology 2021Quote: ... H9 cells were cultured at 37°C and 5% CO2 in feeder-free conditions in Essential 8™ (E8 medium (Gibco, Life Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... heated at 95 °C for 5 min and were run in on 8–16% NuPAGE SDS-PAGE gradient gel (Life Technologies) in MOPS SDS Running Buffer (Life Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... The second half of the data (runs 5-8) was obtained after peptide separation on 75 μm columns packed with 2.6 μm Accucore beads (Thermo Fisher Scientific). Peptides were injected onto 30-40 cm ...
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bioRxiv - Neuroscience 2022Quote: ... They were cultured in 37C with 5% CO2 on geltrex-coated tissue culture plates in Essential 8 complete medium (E8; Gibco, #A1517001) with daily media changes ...
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bioRxiv - Molecular Biology 2023Quote: ... 10μg of total protein were heat-denatured for 5 minutes at 100°C before loading onto NuPAGE 3– 8% TRIS Acetate Midi Gel (Novex, Life Technologies) and transferred to PVDF membranes (Millipore) ...
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bioRxiv - Biophysics 2023Quote: ... against PIEZO1 that targets the 5’-AGCATTGAAGCGTAACAGGG-PAM-3’ at Chr.8: 122513787 - 122513809 on GRCm38 was purchased from Invitrogen (A35533). The cells were transfected with the Cas9 enzyme (TrueCut Cas9 Protein v2 ...
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bioRxiv - Developmental Biology 2023Quote: Single-cell isolated from day 8 organoids (200 unselected organoids) were incubated for 5 minutes at room temperature with Calcein-AM (C3099, Life Technologies) and subsequently sorted on a FACS Aria (BD ...
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bioRxiv - Immunology 2023Quote: Purified CD11c+ myeloid cells (5 × 104 cells) from bone marrow cultures were plated on an 8 well glass chamber slide (Nunc Lab-Tek-Thermo Fisher) in supplemented IMDM medium and incubated for 24 h ...
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bioRxiv - Immunology 2022Quote: SMGs were fixed for 6-8 hours in 4% paraformaldehyde (PFA; Thermo Scientific) at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... freshly passaged undifferentiated cells were plated on hESC-qualified Matrigel (Corning 354277)-coated tissue-culture treated 6-well plates in Essential 8 media (Gibco A1517001) containing 10 uM ROCK inhibitor Y-27632 (Tocris 1254) ...
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bioRxiv - Molecular Biology 2020Quote: ... and shRNA plasmids or pCDH plasmids by PEI-40000 with the ratio of 5: 1: 5 in opti-MEM (Gibco, Thermo Fisher Scientific). Virions were collected after 48 h after transfection ...
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bioRxiv - Immunology 2021Quote: ... mouse interleukin 6 (IL-6; ThermoFisher; 1:500). Sections were then stained for DAPI (Sigma ...
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bioRxiv - Plant Biology 2023Quote: ... using primers GtEFF1 (5’-CCCTGCAAGCTCTTCCTCTTAG-3’) and GtEFR1 (5’-GCATGCGAGGTCCCAAAA-3’) with the TaqMan probe (5’-6FAM-ACTGCACAGACCATC-MGB-3’) (Thermo Scientific™, USA) (Keenan et al. ...
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bioRxiv - Cell Biology 2020Quote: We seeded 2×105 cells / well into 6 well dishes and replaced media with 5% KSR (Knockout Serum Replacement) (Gibco) containing media the next day ...
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bioRxiv - Cell Biology 2020Quote: ... collected into a new tube and stained with 4′,6-diamidino-2-phenylindole (DAPI) (∼5 µg/ml, Molecular Probes # D1306). Round coverslips (Ø12 mm ...
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bioRxiv - Immunology 2021Quote: ... monocyte-derived DCs that were transfected with hACE2 were seeded at 0.5×106 cells/mL in a 6-well plate and transfection was performed with Lipofectamine LTX and PLUS reagents (Invitrogen) according to the manufacturer’s instructions for primary cells ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μg of plasmid was used to transfect 750,000 cells in each well of a 6-well plate using Lipofectamine 2000 (5 μL; Thermo Fisher) in OptiMEM (400 μL ...
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bioRxiv - Neuroscience 2021Quote: NIH-3T3 cells were seeded on 6-well plates and allowed to grow to 70% confluency at 37°C and 5% CO2 in DMEM (Gibco) supplemented with 10% FBS (Life Technologies) ...
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bioRxiv - Cell Biology 2022Quote: 100,000 BHK cells were plated into a 6-well plate and transfected with 5 nM of siRNA pool (siTOOL) against Uhrf1 or Usp7 by Lipofectamine 3000 (Invitrogen). After 24 or 48 hours ...
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bioRxiv - Neuroscience 2019Quote: ... post-axotomy cultures were loaded with lipophilic dye N-(3-trimethylammoniumpropyl) -4-(6-(4-(diethylamino) phenyl) hexatrienyl)pyridinium dibromide (FM 5–95; Invitrogen) using KCl mediated depolarization as described previously (Taylor et al ...
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bioRxiv - Developmental Biology 2019Quote: ... E8 medium was replaced daily and hiPSC were gently passaged every 5-6 days by mechanical selection or bulk passaged using non-enzymatic reagents (i.e., Versene solution (ThermoFisher Scientific) or Phosphate-Buffer-Saline (PBS)-based enzyme-free cell dissociation buffer (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... Droplets of 5 μL cell-Matrigel mixture were manually deposited onto a high performance #1.5 glass bottom 6-well plate (Fisher Scientific). Data acquisition was performed using a confocal microscope (Nikon A1R HD25 ...
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bioRxiv - Bioengineering 2020Quote: ... Primary fibroblast passages 2–6 were achieved in culture in 37°C in a 5% CO2 incubator with DMEM High Glucose (Gibco) with 10% FBS to 80% confluence in 100 mm Petri dishes ...
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bioRxiv - Neuroscience 2021Quote: ... samples were washed 5-6 times over 2 hours in PBT at room temperature and incubated with secondary antibodies (Invitrogen) diluted in NGS-PBT for 24 hours at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Transfection was carried out in unsupplemented NBA using 500 ng of the luciferase reporter construct and 20 ng of a normalizer plasmid pGL4.83-mPGK-hRLuc at 5-6 DIV using Lipofectamine 2000 (Thermo Scientific) with DNA to Lipofectamine ratio of 1:2 ...
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bioRxiv - Cancer Biology 2020Quote: Gene-specific knockdown was achieved by reverse-transfection of PCa cell suspensions (total 5×105 cells) with 12.5 nM siRNA in 6 well plates using RNAiMAX transfection reagent (Life Technologies; Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... HEK 293T cells were seeded at 5×10^6 cells and grown to 70-90% confluence in 10 cm plates in DMEM (Gibco) with 10% (v/v ...
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bioRxiv - Cell Biology 2021Quote: ... 1 µg of plasmid was used to transfect 400,000 cells in each well of a 6-well plate using Lipofectamine 2000 (5 µL; Thermo Fisher) in OptiMEM (400 µL ...
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bioRxiv - Plant Biology 2022Quote: ... and the amplified DNA fragment and an annealed linker oligo (for GFP: primers 5 and 6) were introduced into the pENTR/D-TOPO vector (Invitrogen; sequences were confirmed by Sanger sequencing using primers 7 and 8 ...
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bioRxiv - Cell Biology 2022Quote: ... dissociated neurons were resuspended and cultured at 37°C in a humidified incubator with 5% CO2 and 95% O2 in poly-D-lysine coated 6-well plates in neurobasal media (12349015, Gibco) supplemented with 1% GlutaMAX™ Supplement (35050-061 ...
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bioRxiv - Molecular Biology 2020Quote: ... Trypsinized hippocampi were washed two times with the same HBSS buffer before being triturated 5-6 times with a fire-polished glass Pasteur pipette in BME (Invitrogen) supplemented with 10 percent FBS (VWR) ...
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bioRxiv - Biochemistry 2021Quote: ... the cells were seeded on a 6-well plate with 2 ml of DMEM medium supplemented with 5% horse serum (Gibco). At 24 hours after transfection ...
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bioRxiv - Cancer Biology 2019Quote: The human myeloma cell line KJON (19) was cultured in RPMI with 5 % heat inactivated human serum (HS) and 2 ng/mL interleukin (IL)-6 (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2019Quote: ... PCR was conducted using a 5’ fluorescently-labelled primer (6-FAM, PET, NED, or VIC) (Applied Biosystems, Foster City, CA) and an unlabeled reverse primer ...
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bioRxiv - Neuroscience 2021Quote: ... Longitudinal imaging of vascular leakage in C57BL/6 mouse was performed for 5 days after injecting 600 μg of 3kDa TRITC-dextran (LIF-D-3308, Invitrogen) and 600 μg of 2MDa FITC-dextran (LIF-D-7137 ...
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bioRxiv - Genomics 2020Quote: ... cells were harvested and grown for 5-6 passages in NPC expansion medium (selection medium with the addition of 2% B27 [GIBCO] ...
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bioRxiv - Developmental Biology 2020Quote: Neural retinae were dissected from adult zebrafish dark adapted for 12 hours between 5:30 PM and 6:30 PM and stored in RNAlater (Ambion) at 4°C until extraction ...
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bioRxiv - Microbiology 2021Quote: ... volvulus worm to each well of 6-well plates containing 5 ml of DMEM +10% fetal bovine serum (Gibco, A3840002) + 100 u/ml penicillin + 100 ug/ml streptomycin + sodium bicarbonate (2 g/1) ...
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bioRxiv - Microbiology 2019Quote: ... pelleted again by centrifugation and resuspended at 3−5 × 106 cells/ml in sterile PBS containing 1.25 µg/ml 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI; ThermoFisher Scientific) and stored on ice until required ...
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bioRxiv - Cell Biology 2020Quote: ... The production of ROS was assessed by the oxidation of 5-6-chloromethyl-2,7-dichlorodihydrofluorescein diacetate (CM-H2DCFDA) (Invitrogen Corporation). Cells cultured in 96-well plates were incubated with JC1 ...
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bioRxiv - Cell Biology 2021Quote: ... 1-2 μg of plasmid was used to transfect 500,000 cells in each well of a 6-well plate using Lipofectamine 2000 (5-10 μL; Thermo Fisher) in OptiMEM (400 μL ...