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Citations for Thermo Fisher :
851 - 900 of 10000+ citations for 5 Bromo 2 bromo difluoro methyl 1H benzimidazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... cells were treated with DMNB-caged cAMP (4,5-Dimethoxy-2-Nitrobenzyl Adenosine 3’,5’-Cyclicmonophosphate, Molecular Probes, D1037) for at least 30 minutes prior to imaging at a final concentration of 1 mM ...
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bioRxiv - Molecular Biology 2021Quote: ... and HeLa (ATCC CCL-2) cells were cultured at 37 °C with 5% CO2 in Dulbecco’s modified Eagle’s medium (Invitrogen) supplemented with 10% fetal bovine serum ...
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bioRxiv - Biochemistry 2021Quote: ... by heating at 95 °C for 5 min with 2 µL of SDS-PAGE loading dye (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell proliferation flow cytometry assays were performed using the 5-ethynyl-2’deoxyuridine (EdU) assay kit (Invitrogen #C10634). Cells were incubated with EdU for 24 h and labeled ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were treated with 10 μM of Edu (5-ethynyl-2′-deoxyuridine; Invitrogen™, Thermo Fisher Scientific, USA), and incubated for 30 min ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were treated with 10 μM of Edu (5-ethynyl-2′-deoxyuridine; Invitrogen™, Thermo Fisher Scientific, USA), and incubated for 30 min ...
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bioRxiv - Cancer Biology 2022Quote: ... The reaction was stopped by adding cold blocking buffer (5 mM EDTA, 2% fetal bovine serum (FBS) (Gibco) in HBSS) ...
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bioRxiv - Neuroscience 2024Quote: ... each animal was injected intraperitoneally with 37 mg/kg BW of 5-ethynyl-2′-deoxyuridine (EdU, ThermoFisher, A10044) at 48 hours after surgery ...
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bioRxiv - Neuroscience 2024Quote: ... each animal was injected intraperitoneally with 37 mg/kg BW of 5-ethynyl-2′-deoxyuridine (EdU, ThermoFisher, A10044) at 48 hours after surgery ...
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bioRxiv - Cell Biology 2024Quote: ... The seeded cells were allowed to attach overnight prior to transfection with plasmid DNAs (0.1-0.2 μg/well) using Lipofectamine 2000 (2-5 μL/well; Invitrogen) within a small volume of Opti-MEM (200 μL ...
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bioRxiv - Immunology 2024Quote: ... or BA.5 (2 μg/mL) spike proteins were coated onto 96-well Maxisorp plates (ThermoFisher, Cat. #439454) in 50 μL of 50 mM Na2CO3 pH 9.6 overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... 5% v/v 2-mercaptoethanol) and fractionated by 4–12% Bis-Tris gel (Thermo Fisher Scientific Cat#NP0335BOX) using MES running buffer (Thermo Fisher Scientific Cat#NP000202) ...
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bioRxiv - Cancer Biology 2024Quote: ... washed with PBS and incubated 5 minutes with PBS containing 2 µg/mL Hoechst (33342, Fisher Scientific, UK) and washed in PBS and briefly in ddH2O then mounted on glass slides using ProLong GoldTM anti-fade glass mounting medium (P36980 ...
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bioRxiv - Microbiology 2022Quote: ... and an Acclaim PepMap 100 trap column (100 μm × 2 cm, nanoViper, C18, 5 μm, 100Å; Thermo Scientific), the tryptic peptides were separated by increasing concentrations of 80% acetonitrile (ACN ...
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bioRxiv - Neuroscience 2023Quote: ... 2 x 5 min with 0.05 M PB and mounted on Superfrost Plus (ThermoFisher Catalog# 12-550-15) microscope slides ...
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bioRxiv - Neuroscience 2023Quote: ... in plating media (Neurobasal, Gibco; supplemented with 5% horse serum, Sigma; 2 mM GlutaMAX, Gibco; and 1xGS21, GlobalStem). 500,000 cortical neurons were plated per well of a 6 well plate pre-coated with PDL ...
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bioRxiv - Genetics 2023Quote: ... 3-5 million cells or approximately 15 ug of DNA crosslinked with 2% PFA (Fisher Scientific F79-500) were used as input per reaction ...
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bioRxiv - Cancer Biology 2023Quote: ... and 5% 2-mercaptoethanol] and was resolved by SDS-PAGE on 4% to 12% Bis-Tris gels (Invitrogen). Proteins were transferred to a polyvinylidene difluoride immobilon TM-P membrane (Millipore ...
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bioRxiv - Cell Biology 2023Quote: ... cells were treated with DMNB-caged cAMP (4,5-Dimethoxy-2-Nitrobenzyl Adenosine 3’,5’-Cyclicmonophosphate, Molecular Probes, D1037) for at least 30 min prior to imaging at a final concentration of 1 mM ...
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bioRxiv - Biochemistry 2022Quote: ... 2-3 µL of 5 µM protein solution was introduced directly into Q-Exactive UHMR mass spectrometer (ThermoFisher) through gold coated capillary needles that were prepared in-house30 ...
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bioRxiv - Cell Biology 2023Quote: Embryos were incubated for 1hr at 28ºC with 400 µM 5-ethynyl-2’-deoxyuridine (EdU, Invitrogen Cat# A5040) and 5% DMSO in E3/PTU ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mM disuccinimidyl suberate (DSS) or 2 mM tert-butyl disuccinimidyl phenyl phosphonate (tBu-PhoX)(Thermo Fisher Scientific) in DMSO was added to the solution and incubated for 1 hr at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... organoids were passaged every 1-2 weeks by incubating organoids for 5-10 min in TrypLE Express (Gibco) at 37℃ and mechanical disruption to make organoids fall apart ...
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bioRxiv - Genetics 2023Quote: ... Cells were then blocked with 2 mL blocking solution made of 5% Normal Goat Serum (cat#PCN5000 Thermofisher) and 0.05% Triton X-100 in PBS for 2 hours at RT or 4°C on a shaker.
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bioRxiv - Bioengineering 2024Quote: ... 5 µL of sample were mixed with 2 µL of 1 U/µL DNase I (ThermoFisher Scientific, USA) and 13 µL of 10X DNase I reaction buffer with MgCl2 (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were allowed to attach overnight prior to transfection with plasmid DNAs (0.5-0.75 μg/well) using Lipofectamine 2000 (2-5 μL/well; Invitrogen). Lipofection was done using small volumes of Opti-MEM (200 μL ...
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bioRxiv - Immunology 2024Quote: ... we injected the mice intraperitoneally with 4 mg/ml of 5-ethynyl-2′-deoxyuridine (EdU) (Invitrogen, Waltham, MA) with the goal of labeling circulating monocytes to assess macrophage retention among groups ...
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bioRxiv - Systems Biology 2024Quote: ... followed by reduction and alkylation in 5 mM tris(2 - carboxyethyl)phosphine (TCEP) (Thermo Fisher, Cat No. 77720) and 50 mM 2-Chloroacetamide (CAA ...
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bioRxiv - Cell Biology 2024Quote: ... The same test was done in parallel to assess the cell viability using MTT at 5 ug/mL (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) (Invitrogen). Sixteen sites per well were acquired using a 20x Olympus objective plan fluorite NA 0.45 (1320517 ...
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bioRxiv - Cell Biology 2024Quote: ... 5% v/v 2-mercaptoethanol) and fractionated by 4–12% Bis-Tris gel (Thermo Fisher Scientific Cat#NP0335BOX) using MES running buffer (Thermo Fisher Scientific Cat#NP000202) ...
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bioRxiv - Cell Biology 2024Quote: ... and then nutated in Grace’s Insect Medium containing 0.25 mg/ml 5-ethynyl-2’-deoxyuridine (EdU) (Invitrogen A10044) for 30 min at room temperature to label S-phase cells ...
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bioRxiv - Cancer Biology 2024Quote: ... 2-5 mg of minced tissue was loaded into a cuvette containing Amplex Red (Invitrogen, Waltham, MA, USA), horseradish peroxidase (5U/mL) ...
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bioRxiv - Immunology 2020Quote: ... the cells were stained for 1h with the LIVE/DEAD™ fixable near-infrared dead cell stain (1:1000 dilution, Invitrogen) along with the following labelled antibodies diluted in an ice-cold staining buffer (PBS supplemented with 1% BSA ...
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bioRxiv - Molecular Biology 2020Quote: ... The following secondary antibodies were added for 1h at room temperature: anti-rabbit IgG-647 (1:500, Life technologies, A-21244), anti-rabbit IgG-488 (1:500 ...
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bioRxiv - Genomics 2020Quote: ... Transformed cells were recovered in S.O.C media (from ElectroMAX Stabl4 kit) for 1h and spread on Luria–Bertani agar plates (245×245 mm, Thermo Fisher Scientific) containing 100 µg/mL ampicillin ...
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bioRxiv - Immunology 2021Quote: ... Plates were then washed and incubated for 1h at room temperature with horseradish peroxidase (HRP)-conjugated goat anti-human IgM (Invitrogen #A18835), goat anti-human IgA (Jackson #109-036-011) ...
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bioRxiv - Neuroscience 2020Quote: ... This was followed by washing with PBS three times and incubating for 1h at RT in blocking buffer containing Alexa-Fluor-conjugated isotype-specific secondary antibodies (Thermo Fisher). Cells were washed three times with PBS and DAPI (0.4 μg/ml in PBS ...
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bioRxiv - Physiology 2020Quote: ... Slides were rinsed in PBS 3×5min and then incubated for 1h at room temperature in goat anti-rabbit AF647 (1/250, 21245, Life Technologies) or donkey anti-goat AF647 (1/500 ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then washed 3× with PBS and incubated for 1h with the corresponding Alexa Fluor secondary antibodies (Thermo Fisher Scientific) at 1:1000 dilution (in 3% BSA ...
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bioRxiv - Cancer Biology 2022Quote: ... and 10-20 µg of protein were loaded into 4-12% Bis-Tris Bolt gel and run at 150-200V constant for 1h in Bolt MOPS/MES 1X running buffer (Thermo Scientific). Dry transfer of the proteins to a nitrocellulose membrane was done using IBLOT2 (Thermo Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... 6d ovaries underwent 1h of EdU labelling based on the protocol of the Click-iT™ Plus EdU Cell Proliferation Kit for Imaging (Invitrogen). Both 6d and 12d ovaries were stained for Fasciclin III (Fas3 ...
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bioRxiv - Microbiology 2021Quote: ... Cells were allowed to adhere for 4 hours (+/-1h) before transfection with individual siRNAs (10nM) diluted in a mix of OptiMEM (Life Technologies) and 0.05μL of Interferin reagent (Polyplus Transfection) ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated for 1h with anti-human IgG goat antibody conjugated to FITC or Alexa Fluor 488 (H10301; A-11013, Invitrogen, USA) diluted 1/500 in PBS ...
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bioRxiv - Immunology 2020Quote: ... Plates were then washed and incubated for 1h at room temperature with horseradish peroxidase (HRP)-conjugated goat anti-human IgG (Invitrogen #A18805), goat anti-human IgM (Invitrogen #A18835) ...
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bioRxiv - Microbiology 2020Quote: ... Antibodies/stains were diluted in 2% BSA PBS and samples were incubated for 1h and coverslips were subsequently washed and mounted with ProLong Gold anti-Fade moutant (Thermofisher, P36934). Localisations were visualised on a Ti-E Nikon microscope or Leica SP5 Confocal.
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bioRxiv - Genetics 2021Quote: ... Peptides were resuspended in 0.1% FA and were separated using a 1h gradient on an nLC coupled to a Q Exactive Plus tandem mass spectrometer (Thermo scientific) or an LTQ orbitrap XL mass spectrometer ...
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bioRxiv - Pathology 2020Quote: ... (Extended Data Table 1) and corresponding fluorescently conjugated secondary antibodies (1h at RT) together with 10 μg/ml Hoechst 33342 (trihydrochloride, trihydrate, ThermoFisher Scientific). Sections were mounted with ProLong Gold Antifade mounting medium ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibodies (anti-mouse nsP2 and E2 mAbs) were then added for 1h followed by incubation with Alexa Fluor (AF) 488-conjugated chicken anti-mouse antibody (Invitrogen, USA). Approximately 1×104 cells were acquired by the LSR-Fortessa flow cytometer (BD Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... 1h in Towbin buffer (25mM Tris, 192mM glycine) with 20% methanol) in a wet tank system (Mini Blot Module, Thermo Fisher). The membranes were blocked in 5% dry milk powder in TBS-T (5mM Tris ...
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bioRxiv - Cancer Biology 2021Quote: ... slides were washed and incubated for 1h at RT with 1:200 Alexa-fluor secondary antibodies: goat anti-rabbit 488 (Life Technologies), goat anti-rat 568 (Life Technologies ...