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Citations for Thermo Fisher :
801 - 850 of 10000+ citations for 5 Bromo 2 bromo difluoro methyl 1H benzimidazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... 0.05% saponin at room temperature for 1h followed by Alexa FluorTM 647 goat anti-rabbit IgG (ThermoFisher A21245; 1:1000), 0.05% saponin at room temperature for 1h followed by nuclear staining with Hoechst 33342 (Thermo Fisher Scientific 62249 ...
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bioRxiv - Cancer Biology 2021Quote: ... for 2 hours in the dark at RT followed by 1h incubation with secondary antibodies: anti–mouse Alexa Fluor 488 (1:300, A11001, Invitrogen) and anti–rat Cy3 (1:150 ...
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bioRxiv - Microbiology 2020Quote: ... 0.05% saponin at room temperature for 1h followed by nuclear staining with Hoechst 33342 (Thermo Fisher Scientific 62249; 1:2000) for 15 min ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then incubated with primary antibodies in 10% NGS/NDS in PBST over night at 4°C following 1h incubation at 24°C with appropriate Alexa488/568 coupled secondary antibodies (1:1000, Invitrogen). Cell nuclei were stained with DAPI and final images were acquired using Leica TCS SP8 confocal microscope (Leica Microsystems ...
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bioRxiv - Immunology 2021Quote: from PyMT or wild-type littermate control were cut in small pieces and digested for 1h at 37°C in CO2-independent medium (Gibco) containing 150 μg/mL DNAse and 75 μg/mL Liberase TL (Roche) ...
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bioRxiv - Cancer Biology 2021Quote: ... the slides were washed 3 times with PBS and incubated for 1h at room temperature with goat anti-rabbit AlexaFluor-568 (ThermoFisher, Invitrogen A-1101 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were put back on ice and treated for 1h with anti-Alexa fluor® 488 (#A11094 from Life Technologies) in order to quench remaining surface signal ...
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bioRxiv - Cancer Biology 2021Quote: ... and the cells were serum-starved for 1h by adding 1.5mL Opti-MEM® reduced-serum medium (Gibco, Life Technologies) per well prior to transfection ...
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bioRxiv - Biophysics 2020Quote: ... fixed cells are incubated 1h at room temperature with Myosin 4 Monoclonal Antibody conjugated with Alexa Fluor 488 (MF20, Thermofisher) at 5μg/mL (1:100 dilution ...
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bioRxiv - Cell Biology 2022Quote: ... The sections were blocked in blocking buffer for 1h at room temperature and incubated with BODIPY 558/568 (Invitrogen, D38D35) for 20 minutes ...
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bioRxiv - Plant Biology 2022Quote: ... and immunocomplexes were next incubated for 1h at 4°C with 40 μL of Dynabeads Protein A (Thermo Fisher Scientific). The beads were washed 2 × 5 min in ChIP Wash Buffer 1 (0.1% SDS ...
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bioRxiv - Molecular Biology 2022Quote: ... were incubated for 1h at room temperature in PBS- tween with 2.5% BSA and developed using ECL Western blot reagent (Thermo Scientific).
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bioRxiv - Molecular Biology 2022Quote: ... coverslips were incubated for 1h at room temperature with goat-anti-rabbit Alexa Fluor™ 488 (1:500, ThermoFisher Scientific) as a secondary antibody ...
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bioRxiv - Zoology 2024Quote: ... Excreta were then collected over intervals of 1h-1h30 in 500 µl of DMEM containing 1% antibiotic-antimycotic (Gibco, France). Before adding media ...
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bioRxiv - Microbiology 2024Quote: ... The slides were incubated for 1h at RT with primary antibody (1:20) Mouse Monoclonal Ab anti-AID (ZA001, Invitrogen), in PBS-0.1% BSA-0.4% Tween20 ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were subsequently rinsed and incubated for 1h at RT with the appropriate HorseRadish-Peroxidase (HRP)-conjugated secondary antibodies (Invitrogen). The visualisation of the signal was conducted using Immobilon™ Forte Western HRP Substrate (Millipore ...
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bioRxiv - Immunology 2023Quote: ... The slides were then rinsed 4 times with the washing buffer for 15 minutes and incubated with the secondary antibody at 1:500 dilution in blocking solution for 1h at room temperature (Rabbit 555, Invitrogen, #AB_162543 ...
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bioRxiv - Immunology 2023Quote: ... 100 uL ofmedium from the bottom chamber were collected at either 1h or 4h after Dextran addiction and fluorescence was quantified (Emission; 490, Absorption; 520) on black 96 multiwell plates (Nunc) on CLARIOstar OPTIMA (BMG LabTech ...
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bioRxiv - Biophysics 2022Quote: ... The ligation was desalted by dialysis using membrane filters for 1h and later concentrated 3.3X using a SpeedVac concentrator (Thermo Scientific).
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bioRxiv - Systems Biology 2023Quote: ... samples were incubated for 1h with a goat anti-guinea pig secondary antibody (Alexa Fluor 488 conjugate; 1:1000; Invitrogen) and wheat germ agglutinin to label the nuclear envelope (Alexa Fluor 647 conjugate ...
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bioRxiv - Immunology 2023Quote: ... Coverslips were then incubated for 1h at RT with the appropriate Alexa Fluor conjugated secondary antibody (Invitrogen, 1:300 dilution), incubated for 10 min with DAPI at 1μg/mL in PBS and mounted on slides using Dako Fluorescence Mounting Media.
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bioRxiv - Bioengineering 2023Quote: ... and 0.3% Triton-X solution in PBS for 1h at room temperature and then incubated with a primary rabbit anti-RLuc antibody (1:1000, PA1-180, ThermoFisher) in blocking buffer for overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... sections were boiled in sodium citrate buffer (pH 6.0) for 15 min and incubated with the following antibodies for 1h: anti-CD8 (4SM15, Thermo Scientific), anti-NCR1 (EPR23097-35 ...
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bioRxiv - Neuroscience 2024Quote: ... cells were incubated for 1h at RT with Goat anti-rabbit Alexa-555 conjugated antibody (1:400, Invitrogen, A-21428). For visualization of the total pool of overexpressed KCC2-pHext (Ft ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were blocked for 1h at room temperature in 3% BSA/PBS supplemented with 0.1% Tween 20 (BP337-100, Fisher Scientific), incubated at 4°C overnight with primary antibody in 3% BSA/PBS + 0.1% Tween 20 ...
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bioRxiv - Genomics 2024Quote: ... Cryosections were incubated for 1h in secondary antibody (1:1000 donkey anti-sheep conjugated with AlexaFluor-488; ThermoFisher Scientific (Invitrogen)) ...
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bioRxiv - Genomics 2024Quote: ... Cryosections were incubated for 1h in secondary antibody (1:1000 donkey anti-sheep conjugated with AlexaFluor-488; ThermoFisher Scientific (Invitrogen)) ...
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bioRxiv - Cell Biology 2024Quote: ... incubated for 1h at RT with secondary antibody (4 µg/ml goat anti-rabbit Alexa Fluor 488 (Thermo Fisher Scientific) in PBS-BSA ...
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bioRxiv - Bioengineering 2022Quote: ... The 24-well plates with infected BHK-21 cells were overlaid with 1 ml of 0.8% methylcellulose in complete DMEM medium with 2% FBS and incubated for 5 days in a cell culture incubator (Thermo Fisher Scientific, 37°C and 5% CO2). Plaques were fixed and developed with staining reagent (1% crystal violet in 1:1 methanol/acetone solution ...
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bioRxiv - Neuroscience 2022Quote: ... the brains were immersed in a 1:1-mixture of ethanol and methyl salicylate (Fisher Scientific GmbH, Schwerte, Germany) for 20 min and then in 100% methyl salicylate for about 1 h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... freshly mitochondria isolated from pupae were resuspended in respiration buffer (0.5 mg/ml) containing Tetramethylrhodamine methyl ester perchlorate (0.5 μM) (Thermo Fisher). The excitation spectra were scanned from 520 nm to 580 nm using 590 nm emission wavelengths ...
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bioRxiv - Microbiology 2021Quote: ... and cells were overlayed with carboxy-methyl cellulose (CMC) containing media (0.6% CMC, MEM supplemented with 1X Penicillin/Streptomycin (Gibco), 2% FBS ...
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bioRxiv - Biochemistry 2021Quote: ... The RBD/Legobody complex at 2.5mg/ml were incubated with MS(PEG)12 methyl-PEG-NHS-ester (Thermo Fisher) at a 1:25∼28 molar ratio for 2 hrs on ice ...
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bioRxiv - Microbiology 2024Quote: ... followed by addition of 500 µL of N-methyl-N-trimethylsilyltrifluoroacetamide (MSTFA) with 1% trimethylchlorosilane (TMCS) (Thermo Scientific, TS48915) and heated for 1 h at 70 °C ...
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bioRxiv - Microbiology 2021Quote: ... which was boiled in the SDS loading buffer with 5% β-mercaptoethanol (Cat. #60-24-2, Acros Organics). The denatured protein samples were separated by Novex™ WedgeWell™ 4-20% SDS-PAGE Tris-Glycine gel and transferred to PVDF membrane (iBlot™ 2 Transfer Stacks ...
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bioRxiv - Genomics 2020Quote: ... Here, 40 μL pre-mix (5 μL 10x NEBuffer 2.1, 2 μL 100 mM ATP (Thermo Fisher, R0441), 3 μL 100 mM DTT ...
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bioRxiv - Developmental Biology 2021Quote: ... These vectors were co-transfected into GATA3 reporter cells (G3KI#5-2) using the Lipofectamine Stem reagent (Invitrogen). Two days after transfection ...
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bioRxiv - Immunology 2021Quote: ... we measured the proliferation capacity of NK92 cells by an EdU (5- ethynyl-2’-deoxyuridine; Invitrogen, California, USA) proliferation assay ...
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bioRxiv - Biochemistry 2020Quote: ... and an Acclaim PepMap 100 trap column (100 μm × 2 cm, nanoViper, C18, 5 μm, 100Å; Thermo Scientific) were used to separate tryptic peptides by increasing concentrations of 80% acetonitrile (can ...
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bioRxiv - Microbiology 2021Quote: ... and an Acclaim PepMap 100 trap column (100 µm × 2 cm, nanoViper, C18, 5 µm, 100Å; Thermo Scientific), the tryptic peptides were separated by increasing concentrations of 80% acetonitrile (ACN ...
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bioRxiv - Microbiology 2021Quote: ... the monolayers were overlaid with 2 ml of Minimal Essential Medium (Mediatech) supplemented with 5% FBS (Life Technologies), nonessential amino acids (Gibco) ...
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bioRxiv - Neuroscience 2020Quote: NO production was detected by DAF-FM diacetate (4-amino-5-methylamino-2’,7’-difluorofluorescein diacetate) (D23844, ThermoFisher). Fly larvae were dissected at 24 or 48 h AI in PBS to expose the sensory neurons ...
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bioRxiv - Bioengineering 2022Quote: ... and blocked for 2 hours with a solution containing 0.5% TritonX-100 and 5% donkey serum (Thermo Fisher). Primary antibodies used were rat CD68 (FA-11 ...
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bioRxiv - Microbiology 2020Quote: Cells grown until A600 of ∼0.1 were labelled with 10 μM EdU (5-Ethynyl-2’-deoxyuridine, Thermofisher, C10337) for 15 min after which cells were washed ...
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bioRxiv - Neuroscience 2021Quote: ... Pipettes had resistances of 5–7 MΩ when filled with this solution supplemented with Fura-2 (Molecular Probes). Recordings were made using a Multiclamp 700B amplifier (Molecular Devices ...
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bioRxiv - Immunology 2020Quote: ... the proliferation capacity of NK92 cells was measured by an EdU (5-ethynyl-2 ‘-deoxyuridine, Invitrogen, CA, USA) proliferation assay ...
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bioRxiv - Biophysics 2021Quote: ... Cells were incubated with 10 μM of the synthetic nucleoside 5-Ethynyl-2’-deoxyuridine (EdU) (Thermo Fisher Scientific) for 25 min at 37°C and 5% CO2.
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bioRxiv - Cancer Biology 2020Quote: ... Samples were then treated with ExoSAP-IT enzyme (2 μl of enzyme for 5 μl of sample, ThermoFisher) at 37 °C for 15 min followed by a 15 min inactivation at 80 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... 5-ethynyl-2’-deoxyuridine (EdU)) staining were performed using the Click-iT Plus EdU Assay kit (C10339; Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... Probes consisting of oligodeoxyribonucleotides (see Supplementary Table 2) were 5′-end labeled using T4 polynucleotide kinase (ThermoFisher Scientific) with 25 μCi of [γ-32P]dATP ...