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Citations for Thermo Fisher :
8851 - 8900 of 10000+ citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... followed by staining with 2 µg/mL Hoechst33342 (Molecular Probes) in 200 µL PBS at 4°C for 12 hours ...
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bioRxiv - Cell Biology 2024Quote: ... 2 μl of GlycoBlue Coprecipitant (15 mg/ml, #AM9515; Invitrogen) and 500 μl of isopropanol were added to each sample ...
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bioRxiv - Cancer Biology 2024Quote: ... MCF10A cells were selected using 2 µg/ml Puromycin (Gibco) for two days ...
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bioRxiv - Cancer Biology 2024Quote: ... added with DAPI (4′,6-diamidino-2-phenylindole, Life Technologies, Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... and transferred to iBlot™ 2 Transfer Stacks (Invitrogen #IB23001) using an iBlot 2 Gel Transfer Device (Invitrogen #IB21001) ...
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bioRxiv - Cancer Biology 2024Quote: ... 75 µm × 2 cm Nanoviper Trap 1200BA (Thermo Fisher Scientific) and eluted in an Easy-Spray PepMap RSLC C18 2 µm ...
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 2% (v/v) B-27 (Thermo Fisher Scientific), 2 mM L-glutamine (Gibco) ...
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bioRxiv - Cell Biology 2024Quote: ... warm 2% low melting point agarose (Thermofisher A-204-25) was mixed into 0.08 mg/mL tricaine E3 and anesthetized zebrafish were gently added to the bottom of a 50 mm glass bottom chamber (MatTek) ...
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bioRxiv - Cell Biology 2024Quote: ... 50mL starter cultures of LB media (Fisher Scientific, BP1426-2) with 50mg/mL ampicillin (Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... along with nucBlue nuclear stain (2 drops per mL, Invitrogen).
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bioRxiv - Developmental Biology 2024Quote: ... Slides were then washed in 2× SSC buffer (Invitrogen, 15557044) for 2 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were washed 2 times with TBST and 1 time with TBS before being developed using SuperSignal™ West Femto Maximum Sensitivity Substrate (Thermo Scientific). Membranes were imaged using LAS ImageQuant System (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... and 1 μg of plasmid encoding ER-targeted ESCargo(FTV) per dish using 2 μL of Lipofectamine 2000 reagent (Thermo Fisher; 11668019). Neurons were incubated for 48 h post-transfection and then imaged using a Leica SP5 confocal microscope as described above ...
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bioRxiv - Cell Biology 2020Quote: ... and TagRFP-T-VAPB(1-218)-eMagB or TagRFP-T-eMagB-PHOSBP (prey) and iRFP-P4C at a 3:2:1 ratio in OptiMEM-I (Thermo Fisher Scientific) (1:4 DNA ...
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bioRxiv - Cell Biology 2020Quote: ... labelled with the fluorescent lipid dye BODIPY® FL Maleimide [BODIPY® FL N-(2-Aminoethyl) Maleimide] (Thermo Fisher Scientific; 1:200) and incubated at room temperature in the dark 10 minutes prior to analysis ...
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bioRxiv - Immunology 2021Quote: ... hybridoma cells were adapted to and expanded in serum free medium containing 1:2 vol/vol Cell™ MAB medium (Life Technologies) and HyClone™ CDM4MAB medium (GE Life Sciences) ...
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bioRxiv - Immunology 2021Quote: ... The plasmids were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The two Silencer Select negative control siRNAs (Silencer Select Negative Control No. 1 siRNA and Silencer Select Negative Control No. 2 siRNA) were purchased from Life Technologies (Ambion). In experiments where GBP1-5 were individually knocked down ...
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bioRxiv - Developmental Biology 2020Quote: ... the ovaries were incubated for 2 h at room temperature with secondary Alexa 568-conjugated antirabbit IgG (1:500 in PBX; Invitrogen, Life Technologies), washed and counterstained with Hoescht (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... The plasmids were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... DAB-developed samples were stored in 70% glycerol and counterstained with 4’,6-diamidino-2-phenylindole (DAPI, ThermoFisher Scientific, #D3571, 1:1000) nuclear marker ...
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bioRxiv - Neuroscience 2022Quote: ... and in the secondary antibody diluted in 10% normal donkey serum for 2 hours at room temperature [Secondary antibody: Alexa Fluor 647 goat anti-rabbit (1:500; Thermo Fisher Scientific)].
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bioRxiv - Developmental Biology 2021Quote: ... while for the ECAR measurements the media was supplemented with 1 mM of Sodium Pyruvate and 2 mM of L-glutamine (both from Life Technologies, UK), pH 7.4 at 37°C.
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bioRxiv - Neuroscience 2021Quote: ... 3-3.5 µg prestin plasmids and 0.4 µg eGFP plasmid were transfected to HEK 293 cells using 10 µl of Lipofectamine ® 3000 (reagent 2:1 ratio; ThermoFisher Scientific) in 500-ml Opti-MEM (Life Technologies) ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were washed 3 times with 3X PBS for 10 min/each and 1 time with PBS and incubated for 2 h at room temperature with secondary antibodies - Alexa Fluor 555 donkey anti-sheep at 1:1000 dilution (Invitrogen, cat# A21436), Alexa Fluor 647 donkey anti-mouse at 1:200 dilution (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNA products and DNA products were diluted to 1:10 and 2 μL was used as template for real time PCR reaction with PowerUp SYBR Green (Applied Biosystems, A25779). The primers used for both end point PCR and qRT-PCR analysis are listed in Supplementary Table S1.
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bioRxiv - Molecular Biology 2020Quote: ... which was replaced the next day with human neural induction media (DMEM HAM’s F12, with 50 μg/ml penicillin-streptomycin, 2 mM L-glutamine, 1:100 of N2 (Invitrogen, cat. 17502-048), 1:50 of B27 (Invitrogen ...
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bioRxiv - Bioengineering 2022Quote: ... or SARS-CoV-1-d28 (Genbank AAP13567) or SARS-CoV-2 VOC spike plasmid were incubated with transfection reagent Lipofectamine 2000 (Thermo Fisher, 11668027) following manufacturer’s instructions for 20 min at RT ...
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bioRxiv - Cell Biology 2022Quote: ... Slides were then washed as above and incubated in 1 μg/ml of 4′,6-diamidino-2-phenylindole (DAPI, Thermo Fisher Scientific) at RT for 10 min ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DAPI (4-6-diamidino-2-phenylindole) and DyLight 488-or DyLight 555-labeled secondary antibodies (1:400, Thermo Fisher Scientific, Waltham, MA) were added for 1.5h at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... we used 5 μg plasmid DNA (with a concentration of at least 1 μg/μl) to 2 x 106 cells in R buffer for a 100 μl NEON electroporation pipette tip (Thermo Fisher Scientific) at 1600 V for 30 ms and 1 pulse ...
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bioRxiv - Immunology 2020Quote: ... was then used to electroporate 1-2 million NT or CAR-transduced NK cells using the Neon transfection system (Thermo Fisher Scientific). Alt-R HiFi Cas9 Nuclease 3-NLS (IDT ...
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bioRxiv - Immunology 2021Quote: ... Immunoprecipitated samples were subjected to Proteinase K digestion in wash buffer supplemented with 1% SDS and 2 mg/mL Proteinase K (Thermo Fisher Scientific) incubated with shaking at 1200 rpm at 55□ for 1 h ...
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bioRxiv - Biophysics 2021Quote: ... was introduced into flow cells at 1 ~ 2 μl/min in the T50 buffer with 200 nM Sytox orange (ThermoFisher Scientific; S11368). Excess DNA was washed off with T50 buffer once optimum DNA density was achieved.
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bioRxiv - Immunology 2021Quote: ... were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... The frozen tissue samples were homogenized in 1 ml DMEM-2 manually in a disposable 15 ml closed Tissue Grinder System (Thermo Fisher Scientific). 140 µl out of 1ml samples were used for RNA extraction while 500 µl of homogenates were used for quantification of SARS-CoV-2 ...
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bioRxiv - Cancer Biology 2020Quote: ... PBMC were maintained in complete RPMI 1640 and treated directly with MEDS433 alone or with Dypiridamole (0.1–1 μM) or stimulated with 2% phytohemagglutinin (PHA, Gibco, Thermo Fisher Scientific), IL2 200U/ml (Peprotech ...
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bioRxiv - Cancer Biology 2020Quote: ... Slides were subsequently rinsed in PBS and counterstained with 1:5000 dilution of DAPI (4′,6-Diamidino-2-phenylindole dihydrochloride, Invitrogen, Eugene, OR) in deionized water for 3-5 min at room temperature ...
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bioRxiv - Bioengineering 2021Quote: ... The plasmids were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... N-((6-(2,4-DNP)Amino)Hexanoyl)-1-(BODIPY™ FL C5)-2-Hexyl-Sn-Glycero-3-Phosphoethanolamine (PED-A1) and BODIPY™ FL C5 were purchased from Thermofisher scientific ...
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bioRxiv - Biochemistry 2020Quote: ... after which media was removed and the cell monolayer washed 1 time with 2 ml cold DPBS with calcium and magnesium (DPBS++, Invitrogen, #14040-133). The DPBS++ was removed and 100 ul of ice cold lysis buffer was added containing ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated at RT for 2 hours with secondary antibodies diluted at 1/500 (coupled with Alexa 488 or 568 - Thermo Fisher Scientific) and with Methoxy X04 (5 μM ...
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bioRxiv - Neuroscience 2021Quote: ... hMOs were then washed overnight with 1X PBS at 4°C and incubated with Hoechst 33342 solution in 1X PBS for 2 h (1:1000, Thermo Fisher Scientific) and washed in PBS for 2 h ...
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bioRxiv - Neuroscience 2020Quote: ... with 1:1000 dilution in staining buffer for 2 hours at room temperature and stained with DAPI (300 nM, D1306, Thermo Fisher Scientific) for 10 minutes at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... washed 3 times with blocking buffer and incubated for 2 h with Alexa 647 anti-rabbit (1/250, A21247, Thermo Fisher Scientific). After washing the cells 3 times with PBS ...
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bioRxiv - Biochemistry 2021Quote: ... approximately 1 pmol injected onto a Acclaim PepMap 100 guard column (75 μm×2 cm C18, 3 μm particles, 100 Å; Thermo Scientific) and separated on a 50 cm PepMap RSLC C18 (75 μm×50 cm ...
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bioRxiv - Microbiology 2020Quote: Two milli-litres of O+ erythrocytes at 2% hematocrit in incomplete-culture media were labelled using 2 µl of a stock of 1 mM Far-red Cell Trace (Thermo Fisher Scientific); control unstained erythrocytes were incubated with 2 µl of Dimethyl-sulphoxide (DMSO ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 2 hours at RT with appropriate secondary antibodies: goat anti-rabbit Alexa Fluor633 (1:200, ThermoFisher, A-21070), and donkey anti-mouse Alexa Fluor488 (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were subsequently incubated in the dark for 2 hours at room temperature with secondary antibody donkey anti-rabbit IgG conjugated to AlexaFluor-594 (1:250; Invitrogen, A-21207). After washing with PBS ...
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bioRxiv - Microbiology 2020Quote: ... The plasmids were transiently co-transfected into Expi293F cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ 293 Reagent (Thermo Fisher Scientific) according to the manufacturer” s instructions ...