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Citations for Thermo Fisher :
8801 - 8850 of 10000+ citations for C Reactive Protein Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 min organic gradient ...
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bioRxiv - Microbiology 2021Quote: ... The resulting peptides were fractionated using an Ultimate 3000 nano-LC system in line with an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific). In brief ...
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bioRxiv - Microbiology 2021Quote: The LC-MS/MS analysis of TMT-labelled peptides were performed on an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific) coupled with a Thermo Scientific Ultimate 3000 RSLCnano UHPLC system (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2020Quote: ... The application of a 3.5 kV distal voltage electrosprayed the eluting peptides directly into a Q Exactive HF mass spectrometer equipped with an EASY-Spray source (Thermo Scientific). Mass spectrometer-scanning functions and HPLC gradients were controlled by the Xcalibur data system (Thermo Scientific) ...
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bioRxiv - Immunology 2020Quote: ... both preceded by a murine IgK leader peptide and followed by an 8xHis Tag were synthetized and cloned into pcDNA™3.1/Zeo(+) expression vector (Thermo Fisher). Proteins were produced by transient co-transfection of exponentially growing Freestyle™ 293-F suspension cells (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... The eluted peptides from the C18 column were analyzed using a Q-Exactive Plus Orbitrap MS (Thermo Scientific, San Jose, CA) for high resolution MS and high-energy collision-induced dissociation tandem MS by electrospray ionization ...
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bioRxiv - Plant Biology 2021Quote: ... the peptides were dissolved in 4 μL of 0.2% formic acid (FA) and injected into an Easy-nLC 1200 (Thermo Fisher Scientific). Peptides were separated on a 15 cm in-house packed column (360 μm OD × 75 μm ID ...
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bioRxiv - Plant Biology 2021Quote: ... Total peptide samples and the immuno-enriched fractions were then analyzed via LC-MS/MS using an EASY-nLC 1200 (Thermo Fisher) coupled to a Q Exactive HF mass spectrometer (Thermo Fisher) ...
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bioRxiv - Plant Biology 2020Quote: ... The lyophilized peptide was dissolved in nuclease-free water and its concentration determined on a Nanodrop 2000c (Thermo Scientific, Waltham, MA) using A280 with the molar extinction coefficient (3230 M-1cm-1 ...
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bioRxiv - Microbiology 2021Quote: ... The resulting peptides were collected on a trap column (2 mm x 2 cm) filled with POROS 20 R2 material (Thermo Scientific) kept at 0.5 °C ...
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bioRxiv - Microbiology 2019Quote: Eluting peptides were converted to gas-phase ions by means of electrospray ionization and analysed on a Thermo Orbitrap Fusion (Thermo Scientific). Survey scans of peptide precursors from 375 to 1500 m/z were performed at 120K resolution (at 200 m/z ...
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bioRxiv - Microbiology 2020Quote: ... Resulting peptides were analyzed by online nanoliquid chromatography coupled to tandem MS (UltiMate 3000 and LTQ-Orbitrap Velos Pro, Thermo Scientific). Peptides were sampled on a 300 μm x 5 mm PepMap C18 precolumn and separated on a 75 μm x 250 mm C18 column (PepMap ...
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bioRxiv - Cell Biology 2021Quote: ... the peptides were resuspended in 3% formic acid (FA)/5% acetonitrile (ACN) and loaded onto a nanoLC system (RSLCnano, Thermo Scientific). First ...
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bioRxiv - Microbiology 2020Quote: ... Eluting peptide fragments were ionized by electrospray ionization and analyzed on a LTQ-XL Orbitrap Discovery hybrid instrument (Thermo Fisher Scientific). The LC–MS/MS procedure was performed according to the data-dependent acquisition (DDA ...
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bioRxiv - Microbiology 2020Quote: ... The experimental setup was as follows: the peptide mixture was loaded onto a 75 μm × 2 cm precolumn (Acclaim PepMap 100, C18, 3 μm, Thermo Scientific) and eluted over 60 min using a 75 μm × 25 cm analytical column (Acclaim PepMap RSLC ...
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bioRxiv - Cell Biology 2021Quote: Tryptic peptides were analysed using an EASY-nLC 1000 coupled to a Q Exac-tiveTM Hybrid Quadrupole-Orbitrap (Thermo Fisher Scientific). The peptides were resolved and separated on a 50cm analytical EASY-Spray column equipped with pre-column over a 120 min gradient ranging from 8 to 38% of 0.1% formic acid in 95% acetonitrile/water at flowrate of 2 00nl/min ...
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bioRxiv - Cell Biology 2021Quote: ... Affinity enriched proteins were digested with trypsin and the resulting peptides desalted prior to LCMS analysis using an Easy-nLC 1000 system (Thermo Scientific) connected to a Q Exactive HF-X mass spectrometer (Thermo Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... The peptides were trapped on a C18 PepMap 100 pre-column (300 μm i.d. x 5 mm, 100Å, Thermo Fisher Scientific) using solvent A (0.1% formic acid in water) ...
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bioRxiv - Cell Biology 2020Quote: The fractionated peptides were analysed by LC-MS/MS using a fully automated Ultimate 3000 RSLC nano System (Thermo Fisher Scientific) fitted with a 100 μm x 2 cm PepMap100 C18 nano trap column and a 75 μm×25 cm ...
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bioRxiv - Cancer Biology 2022Quote: ... The separated peptides were then ionized using a nanospray ionization ion source and analyzed using an Orbitrap Fusion™ (Thermo Scientific) mass spectrometer ...
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bioRxiv - Cancer Biology 2022Quote: ... The peptides were eluted from the pre-column onto an Easy-spray (25 cm x 75 μm; Thermo Fisher Scientific, DE) column into the Orbitrap Exploris 480 mass spectrometer ...
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bioRxiv - Cancer Biology 2022Quote: Tryptic peptides were analysed using an EASY-nLC 1000 coupled to a Q Exactive™ Hybrid Quadrupole-Orbitrap (Thermo Fisher Scientific). The peptides were resolved and separated on a 50 cm analytical EASY-Spray column equipped with pre-column over a 120 min gradient ranging from 8 to 38% of 0.1% formic acid in 95% acetonitrile/water at a flow rate of 200nl/min ...
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bioRxiv - Biochemistry 2022Quote: ... Desalted peptides were solubilized in solvent A (0.1% aq. formic acid (FA)) and 1µg (determined by NanoDrop (Thermo Scientific Bremen, Germany)) was loaded and separated on an EASY-nLC (Thermo Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... and then 1 μg of each peptide sample was loaded on a rap column (75 µm × 2 cm, 3 µm, C18, Thermo Fisher). The eluate was loaded onto a reversed-phase analytical column (50 µm × 250 mm ...
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bioRxiv - Biochemistry 2022Quote: ... Peptide-RNA adducts were separated using a PepMap RSLC column (250 mm × 75 um, 2 µm particle size, Thermo Fisher Scientific) with gradient of 6-40% mobile phase B (A= water:ACN:FA ...
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bioRxiv - Biochemistry 2022Quote: ... Peptide-RNA adducts were separated on a PepMap RSLC column (150 mm × 75 um, 2 µm particle size, Thermo Fisher Scientific) using a gradient of 5-30% mobile phase B (A= water:ACN:FA ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... the peptides from each sample were reacted for 1 h with the appropriate tandem mass tag (TMT) isobaric reagent (Thermo Fisher) in 40% (v/v ...
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bioRxiv - Molecular Biology 2022Quote: The resulting peptides were fractionated using an Ultimate 3000 nano-LC system in line with an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific). In brief ...
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bioRxiv - Cancer Biology 2022Quote: ... Aliquots containing 0.5 μg peptides were separated on a reverse phase C18 column and were analyzed on QExactive HF Mass Spectrometer (Thermo-Fisher Scientific) as described previously 88 ...
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bioRxiv - Molecular Biology 2022Quote: ... The nTAP-MS peptides were applied on an Acclaim PepMap 100 C18 trap column (75 μm ID x 2 cm, 3 μm, Thermo Scientific) in 0.1% formic acid ...
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bioRxiv - Neuroscience 2022Quote: ... then the flow-throughs were fractionated into 8 fractions using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (ThermoFisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... Resulting peptides were analyzed by online nanoliquid chromatography coupled to tandem MS (UltiMate 3000 RSLCnano and Q-Exactive Plus, Thermo Scientific). Peptides were sampled on a 300 µm x 5 mm PepMap C18 precolumn and separated on a 75 µm x 250 mm C18 column (Reprosil-Pur 120 C18-AQ ...
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bioRxiv - Plant Biology 2022Quote: ... Tryptic peptides were resuspended in H2O containing 0.1% formic acid and separated in an EASY-nLC1000 chromatographic system (Thermo Fisher Scientific) for 95 minutes using a C18 nano-column under a constant flow of 400 nL/min and increasing concentrations of acetonitrile (7 to 30% over 70 min and then 70% in 25 min) ...
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bioRxiv - Molecular Biology 2022Quote: ... The peptides were concentrated (online) by reverse phase chromatography using a 0.1 mm × 20 mm C18 RP precolumn (Thermo Fisher Scientific), and then separated using a 0.075mm x 250 mm C18 RP column (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... was cloned in frame with a N-terminal GFP tag and intervening short peptide linker (GSGEGRG) into the pcDNA5/FRT/TO bacterial expression vector (Invitrogen; V652020). A C-terminal histidine tag (Hisx6 ...
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bioRxiv - Biochemistry 2022Quote: ... Relative ion abundance of peptides labelled with 12C-IPA and/or 13C-IPA in extracted ion chromatograms generated using XCalibur Qual Browser software (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... the captured viral biotinylated peptides were detected with 1 mg/ml pNPP (p-nitro-phenyl-phosphate, Thermo Fisher Scientific, cat: 34045) diluted in Diethanolamine substrate buffer 1X (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: The full-length CcdA and CcdA peptides of different lengths (Table S1) were biotinylated with EZ-linkTM NHS-Biotin (ThermoFisher Scientific) as per the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... peptides were labeled in DMSO using ∼1.3-fold molar excess of Oregon Green 488 maleimide (Life Technologies/Molecular Probes #O6034, USA) dissolved in a 1:1 solution of acetonitrile:DMSO ...
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bioRxiv - Biochemistry 2022Quote: ... peptides were labeled in DMSO using ∼1.3-fold molar excess of Oregon Green 488 maleimide (Life Technologies/Molecular Probes #O6034, USA) dissolved in a 1:1 solution of acetonitrile:DMSO ...
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bioRxiv - Cell Biology 2022Quote: ... An EASY nLC ultra-pressure liquid chromatography system was used to elute peptides onto a Fusion Tribrid mass spectrometer (Thermo Scientific). MS1 profiling was performed in a 375–1600 m/z range at a resolution of 70000 ...
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bioRxiv - Cell Biology 2022Quote: ... Peptides were loaded onto a μ-precolumn (Acclaim PepMap 100 C18, cartridge, 300 μm i.d.×5 mm, 5 μm) (Thermo Scientific), and were separated on a 50 cm reversed-phase liquid chromatographic column (0.075 mm ID ...
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bioRxiv - Cell Biology 2022Quote: ... In brief, peptides were loaded on a C18 PepMap100 pre-column (300 µm i.d. x 5 mm, 100Å (Thermo Fisher Scientific)) at a flow rate of 12 μl/min in 100% buffer A (0.1% formic acid in H20) ...
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bioRxiv - Neuroscience 2021Quote: ... Phospho-LIMK1/2 was detected with rabbit polyclonal anti-phospho-LIMK1 Thr508/ LIMK2 Thr505 directed against a synthetic phospho-peptide (Thermo Fisher Scientific # PA5-37629 ...
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bioRxiv - Biochemistry 2021Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 minute organic gradient ...
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bioRxiv - Microbiology 2020Quote: ... The resulting tryptic and chymotryptic peptides were fractionated using an Ultimate 3000 nano-LC system in line with an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific). In brief ...
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bioRxiv - Molecular Biology 2020Quote: Sample aliquots comprising 25 μg of peptides were labelled with isobaric tandem mass tags (TMT 10-plex, Thermo Fisher Scientific, 90110) as described previously31 ...
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bioRxiv - Molecular Biology 2020Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 min organic gradient ...
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bioRxiv - Molecular Biology 2020Quote: ... Peptides extracted from each band were analyzed by nanoLC-MS/MS using an Ultimate 3000 system (Dionex, Thermo Scientific, Waltham, MA) coupled to a TripleTOFTM 6600 mass spectrometer (ABSciex) ...
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bioRxiv - Cell Biology 2020Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 min ...