Labshake search
Citations for Thermo Fisher :
8601 - 8650 of 10000+ citations for C Reactive Protein Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2024Quote: ... The protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Cat#23225).
-
bioRxiv - Biochemistry 2024Quote: ... Protein concentrations in cell lysates were normalized using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific), according to the manufacturer’s instructions ...
-
bioRxiv - Genetics 2024Quote: ... Precipitation of the protein-antibody conjugate was performed with DynabeadsTM Protein G (10003D, Thermo Fisher Scientific™). Chromatin-bead complexes were washed three times with Low-salt buffer followed by one wash with 1 ml High-salt buffer and eluted in TES buffer (50 mM TRIS-HCl pH 8 ...
-
bioRxiv - Neuroscience 2024Quote: ... The protein concentration was established with the BCA Protein Assay kit (#23227, Thermo Fisher Scientific, Rockford, USA).
-
bioRxiv - Neuroscience 2024Quote: ... The protein concentration was calculated using the BCA Protein Assay Reagent (23225, Pierce™ Thermo Fisher Scientific). Protein samples were separated by SDS-PAGE (Thermo Fisher Scientific ...
-
ATP-dependent citrate lyase Drives Left Ventricular Dysfunction by Metabolic Remodeling of the HeartbioRxiv - Molecular Biology 2024Quote: ... Proteins were separated on 4-12% SDS-PAGE gels (Precast Protein gels, Cat#NP0322BOX, Invitrogen, Waltham, MA), transferred to PVDF membranes and probed with antibodies from Cell Signaling Technology (CS ...
-
bioRxiv - Immunology 2024Quote: ... Total protein concentrations were determined using a Pierce™ BCA protein assay kit (Thermofisher Scientific, Cat # 23225). An equal amount of protein was loaded for SDS-PAGE on 10% polyacrylamide gels ...
-
bioRxiv - Molecular Biology 2024Quote: ... The protein concentration of cell lysates was also determined using a BCA protein assay kit (Thermo Scientific) and data presented depicts caspase-3/7 activity as relative fluorescence unit (RFU ...
-
bioRxiv - Neuroscience 2024Quote: ... Protein concentration was determined through the Pierce BCA Protein Assay Kit following the manufacturer’s instructions (Thermo Scientific) and 25 μg of each sample was prepared for SDS-PAGE ...
-
bioRxiv - Immunology 2024Quote: ... Protein concentrations from individual samples were quantified using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) and luciferase values were normalized to protein.
-
bioRxiv - Plant Biology 2024Quote: ... Total protein content was quantified using the Pierce™ 660nm Protein Assay Kit (©Thermo Scientific™). Following quantification ...
-
bioRxiv - Cancer Biology 2021Quote: ... and RES-C cells were cultured in a humidified CO2-incubator at 37°C in Gibco™ RPMI 1640 (1X) media (Thermo Fisher Scientific) supplemented with 10% FBS (Gibco standard FBS ...
-
bioRxiv - Genomics 2019Quote: ... Samples were heat-denatured (95°C/ 5 min) and incubated o/n at 37°C with 150 U of λ-exonuclease (λ-exo; Thermo Scientific) in the presence of 40 U of RNAseOUT ...
-
bioRxiv - Microbiology 2019Quote: ... with 40 cycles of 15 s at 95°C followed by 1 min at 61°C (Applied Biosystems™ 7500 Real-Time PCR). Abundance was expressed as the -Ct (or the log2A(t)) ...
-
bioRxiv - Plant Biology 2021Quote: ... and subsequently cloned into the destination vector pGWB517 (35S pro, C-4xMyc) or pGWB541 (35S pro, C-EYFP) (Nakagawa et al., 2007) via the Gateway LR reaction (Invitrogen, Carlsbad, CA, USA). Both constructs were transformed into the Agrobacterium tumefaciens strain GV3101 and transferred to rls6 mutant plants via floral dipping as described above ...
-
bioRxiv - Cancer Biology 2021Quote: ... to 125°C for 30 seconds and 90°C for 10 seconds in citrate (pH = 6.0, Thermo Scientific cat. no. Ap-9003-500) for Ascl1 and Zfp36l1 staining ...
-
bioRxiv - Microbiology 2020Quote: ... The samples were then centrifuged for 3 minutes at 10000 RPM and heated for 10 minutes at 100°C in an Eppendorf Thermomixer C (Thermo Fisher Scientific, USA) to lyse the bacterial cells ...
-
bioRxiv - Genomics 2021Quote: ... anchored degenerate octamer primers were annealed with a 65°C to 4°C incubation followed by first-strand synthesis with SuperScript™ III (Thermo Fisher Scientific) and 33 cycles of polymerase chain reaction with Platinum® Taq DNA Polymerase (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2021Quote: ... CMSC29 cells were cultured in 85% AmnioMAX™ C-100 basal medium and 15% AminoMAX™ C-100 supplement (Invitrogen™, ThermoFisher Scientific). DMSC23 cells were cultured in MesenCult™ MSC Basal medium (Human) ...
-
bioRxiv - Cell Biology 2021Quote: ... CMSC29 cells were cultured in 85% AmnioMAX™ C-100 basal medium and 15% AminoMAX™ C-100 supplement (Invitrogen™, ThermoFisher Scientific). DMSC23 cells were cultured in MesenCult™ MSC Basal medium (Human) ...
-
bioRxiv - Developmental Biology 2022Quote: ... 95 °C for 5 seconds and 60 °C for 20 seconds in a QuantStudio 6 Flex Real-Time PCR machine (Thermo Fisher Scientific, USA). The detectors used were FAM-BHQ1 and HEX- BHQ1 ...
-
bioRxiv - Molecular Biology 2023Quote: ... 45 cycles of cyclic denaturation and annealing at 95°C for 15 secs and 60°C for 60 secs respectively were used in QuantStudio3 Real-Time PCR System (Thermo Fisher Scientific, Singapore). The miRNA expression levels were calculated using the 2-ΔΔCt method [38]and were normalized to the U6 expression) ...
-
bioRxiv - Neuroscience 2023Quote: ... SAP97 or SAP102 fused to a C-terminal MBP were coated overnight at 4°C on ELISA plates (Nunc Maxisorb Immunoplates, #442404, Thermo) at a concentration of 100 ng per well ...
-
bioRxiv - Cancer Biology 2024Quote: ... The obtained cell lysates were spin for 10 min of centrifugation at 20,000 × g at 4°C and the collected cell lysate supernatants were pre-absorbed with C-1 magnetic beads (Thermo Fisher Scientific, #65002) for 1 h at room temperature before proceeding to oligo probe hybridization ...
-
bioRxiv - Neuroscience 2023Quote: ... cells were centrifuged (300rcf, 7min, 4°C) and fixed (1h, 4°C) using the eBioscience Foxp3/Transcription factor staining buffer set (ThermoFisher #00-5523-00) fixation/permeabilization reagent ...
-
bioRxiv - Immunology 2024Quote: ... followed by 40 cycles of denaturation at 95 °C for 30 sec and extension at 60 °C for 30 sec in the QuantStudio 6 system (Applied Biosystems Co., USA). At the end of PCR ...
-
bioRxiv - Immunology 2024Quote: ... followed by 40 cycles of denaturation at 95 °C for 5 sec and extension at 58 °C for 34 sec in the QuantStudio 6 system (Applied Biosystems Co., USA). At the end of PCR ...
-
bioRxiv - Microbiology 2023Quote: ... a mammalian secretion sequence and a C-terminal C- tag) was transiently expressed using Expi293F™ cells using the Expi293™ Expression System Kit (Thermo Fisher) as recommended ...
-
bioRxiv - Biophysics 2023Quote: ... Transfection lasted for 6 hours at 37°C and was terminated by replacing the medium with fresh 37°C Opti-MEM® (Life Technologies, ThermoFisher Scientific) supplemented with 50 μg/mL Gentamycin (Invitrogen) ...
-
bioRxiv - Genomics 2023Quote: ... were thawed in a 37 °C water bath for 3 minutes and then mixed with 37 °C thawing media containing: RPMI Medium 1640 (Life Technologies #11875-085) supplemented with 5% heat-inactivated fetal bovine serum (Life Technologies #16000044) ...
-
bioRxiv - Systems Biology 2023Quote: ... and incubated at 97°C for 40 min and cooled down to 65°C using Lab vision PT module (Thermofisher Scientific, Waltham, MA). Slides were washed for 5 min with a wash buffer made with ultra-pure DNase/RNase-Free water (Bio-Lab ...
-
bioRxiv - Systems Biology 2023Quote: ... and incubated at 97°C for 40 min and cooled down to 65°C using Lab vision PT module (Thermofisher Scientific, Waltham, MA). Slides were stained using Akoya Staining Kit for Phenocycler (Akoya Biosciences ...
-
bioRxiv - Cancer Biology 2021Quote: ... coverslips were incubated for 1 hour at room temperature in blocking buffer containing the following secondary antibodies: Goat anti-Rabbit Alexa Fluor® 594 conjugate (1:250, #A-11037, Life Technologies) and Donkey anti-Mouse Alexa Fluor® 488 conjugate (1:250 ...
-
bioRxiv - Cancer Biology 2021Quote: ... Slides were permeabilized with 0.5% Triton X-100 in DPBS for 10 minutes and incubated in BlockAid Blocking Solution (Thermo Fisher, Cat# B10710) for 1 h at RT in a humidity chamber and incubated O/N in primary antibody ...
-
bioRxiv - Cell Biology 2020Quote: ... NRK cells expressing WT importin α or mutant importin α S62A released from thymidine blocking were changed to CO2-independent medium (Invitrogen), 10% cosmic calf serum (HyClone) ...
-
bioRxiv - Immunology 2021Quote: ... Secondary antibody was diluted to 1:3000 in blocking buffer and added to the plates for 60 minutes followed by TMB substrate (Thermo Fisher Scientific). Upon stopping the reaction with 1 M H2SO4 ...
-
bioRxiv - Neuroscience 2021Quote: ... Cryosections of the cochlea and brain slices were washed in PBS for 1 h and subsequently incubated in secondary antibody solution containing blocking solution and the secondary antibodies Alexa Fluor 488-conjugated goat anti-rabbit (1:1000, cochlear cryosections) (Molecular Probes (A11008)) ...
-
bioRxiv - Developmental Biology 2021Quote: ... The samples were then washed three times with 1xPBS and incubated with Alexa 594 conjugated anti-mouse IgG antibody (1:500 in blocking buffer, ThermoFisher Scientific, A11012) and Alexa 488 conjugated anti-rabbit IgG antibody (1:500 in blocking buffer ...
-
bioRxiv - Neuroscience 2021Quote: ... sections were incubated for one hour on the rocker at room temperature in blocking solution with 1:1000 Goat anti-Rabbit IgG H&L Alexa Fluor 405 (Thermo Fisher Scientific). Then sections were washed once in PBS-T ...
-
bioRxiv - Neuroscience 2019Quote: ... sections were washed 3×5mins in PBS and incubated for two hours in appropriate secondary antibodies (Invitrogen; 1:250 in blocking solution). For double labelling of Iba1 and GAD67 ...
-
bioRxiv - Bioengineering 2019Quote: ... cardiomyocytes were incubated in blocking solution containing Alexa Fluor Plus 488-conjugated goat anti-mouse IgG (1:300, A32723; Thermo Fisher Scientific) for 60 min ...
-
bioRxiv - Cancer Biology 2019Quote: ... Cells were rinsed three times with PBS and blocking buffer with AlexaFluor 488-coupled goat anti-mouse antibody (Life Technologies, 1:1000) and 0.8 μg/mL DAPI (4,6-diamidino-2-phenylindole ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were blocked in blocking buffer (1% BSA, PBST) for 1h at room temperature and incubated with primary antibody LC3A/B (Thermo Scientific, USA) overnight at 4 °C ...
-
bioRxiv - Plant Biology 2020Quote: ... Slides were washed three times for 5 min in 1X PBS solution and then incubated for 3 h at room temperature in 100 μL blocking buffer containing Alexa 488-conjugated goat anti-rabbit secondary antibody (Molecular Probes, Invitrogen), diluted 1:200 in fresh blocking buffer ...
-
bioRxiv - Neuroscience 2021Quote: ... After rinsing in PBS and incubating in blocking buffer (10% normal goat serum, Cat #PCN5000 and 0.2% Triton™ X-100 Cat #9002-93-1, ThermoFisher, in PBS) for 30 mins ...
-
bioRxiv - Neuroscience 2021Quote: ... Sections were then washed 3 x 10 min in PBS and incubated in goat anti-rabbit Alexa Fluor 647 (1:500 in blocking buffer; catalog no. A32733, Invitrogen, Carlsbad, CA) for 2 hr at RT ...
-
bioRxiv - Biochemistry 2021Quote: ... washed 3 times with blocking buffer and incubated for 2 h with Alexa 647 anti-rabbit (1/250, A21247, Thermo Fisher Scientific). After washing the cells 3 times with PBS ...
-
bioRxiv - Microbiology 2020Quote: ... Plates were washed with TBS twice before incubating with serially diluted mAbs and CD81-LEL-hFc (R& D systems) in casein blocking buffer (Thermo Fisher Scientific) for 90 min ...
-
bioRxiv - Molecular Biology 2021Quote: ... coverslips were washed 3 times in PBS + 0.1% Tween then incubated at room temperature in the dark for 1 hour in blocking buffer containing Alexa Fluor 546 goat anti-rabbit IgG (Invitrogen, A-11035) secondary antibody (1:1500 ...
-
bioRxiv - Genomics 2021Quote: ... and then blocked for 1 hour with 1 ml of blocking buffer (wash buffer containing 3% fatty acid free BSA (Thermo Fisher, 126609)) ...