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Citations for Thermo Fisher :
8651 - 8700 of 10000+ citations for Recombinant Rabbit CXCL12 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... HK-2 cells were maintained in keratinocyte serum-free (KSF) media with 0.05 mg/mL bovine pituitary extract and 5 ng/mL human recombinant epidermal growth factor (Gibco - Thermo Fisher Scientific, MA, USA). All culture conditions contained a 5% antibiotic / antimycotic mixed solution (Nacalai Tesque) ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then co-transfected with recombinant plasmid (300 ng) together with miR-544-3p mimic or negative control mimic (50 nM) using Lipofectamine 3000 (Thermo Fisher Scientific, Waltham, MA) and Lipofectamine RNAimax (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... at a bead-to-cell ratio of 1:1 in the presence of recombinant IL-4 (Fisher Scientific, #204-IL-010, 10 ng/ml) and anti-IFN-γ antibody (10 μg/ml) ...
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bioRxiv - Cancer Biology 2020Quote: ... Recombinant human IFNγ (285-IF) was purchased from R & D Systems (Minneapolis, MN) and mouse IFNγ (BMS326) from eBioscience (Thermo Fisher Scientific, Waltham, MA). The following antibodies were used for Western blot ...
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bioRxiv - Microbiology 2019Quote: ... Purified cells were cultured in a tissue culture flask containing 20 mL RPMI 1640 supplemented with recombinant murine Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) (10 ng/mL) (BioSource, Invitrogen cytokines & signaling, Camarillo, CA). At day 3 of the culture ...
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bioRxiv - Cell Biology 2020Quote: ... Recombinant adenoviral vectors were produced and purified using HEK293A cells according to manufacturer’s protocol (ViraPower Adenoviral Expression System; Invitrogen, AdenoPACK 20, Vivapure; Sartorius AG).
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bioRxiv - Cell Biology 2020Quote: ... Clarified lysates were then incubated with 20 μg of recombinant glutathione S-transferase (GST)-RBD-MICAL-L1 and Pierce™ Glutathione magnetic agarose beads (Life Technologies-Invitrogen, cat# 78601) for 2 hrs at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... were prepared from 10 to 11-day-old chicken embryos (SPF eggs, VALO, Cuxhaven, Germany) using recombinant trypsin (Tryple TM, Thermo Fisher Scientific, Planegg, Germany) and maintained in VP-SFM medium ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were replated onto glass coverslips at day 12 of the differentiation in N2/B27 medium supplemented with four recombinant growth factors at 25ng/ml (BDNF; ThermoFisher, NT3, NGF, GDNF; Peprotech). CHIR90221 was included for 4 further days ...
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bioRxiv - Cancer Biology 2024Quote: ... PWR-1E and PIN cells were cultured in Keratinocyte Serum-Free Medium supplemented with bovine pituitary extract and recombinant human epidermal growth factor (Gibco, Life Technologies, Grand Island, NY). Myc-CaP ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5% fetal bovine serum except for RWPE-1 and RWPE-2 cell lines which were grown in a keratinocyte serum-free medium supplemented with 5 ng/mL recombinant human epidermal growth factor (EGF) and 50 μg/mL bovine pituitary extract (Gibco™, Invitrogen, Carlsbad, CA, USA). The cell lines were tested for short tandem repeat (STR ...
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bioRxiv - Microbiology 2024Quote: ... and cultured in Gibco® keratinocyte serum-free medium (K-SFM) supplemented with recombinant human EGF and bovine pituitary extract (Thermo Fisher Scientific, #17005075). The cells were propagated in 75cm2 flasks (Falcon ...
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bioRxiv - Cell Biology 2024Quote: Organoids were treated from day 4 through day 11 for EN1 and day 7 through day 11 for EPC2 with or without 10 ng/mL recombinant IL-13 (200-13, Thermo Fisher Scientific, Waltham, MA), reconstituted in phosphate-buffered saline (PBS ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated with the fluorescent secondary antibodies (Alexa Fluor 568-conjugated anti-rabbit IgG, 1:500, Invitrogen, A11011; 488-conjugated anti-rabbit IgG, Invitrogen A21206) for 3 h at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... were detected by immunofluorescence assay after acetone fixation using a polyclonal rabbit anti-PUUN serum diluted 1:1000 in PBS followed by AlexaFluor488-conjugated donkey anti-rabbit secondary antibody (Thermo Scientific). Nuclei were stained using Hoechst 33420 diluted 1:5000 in PBS ...
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bioRxiv - Immunology 2019Quote: ... cells were blocked with 1% BSA at room temperature and incubated with primary rabbit anti-occludin antibody and secondary FITC-conjugated goat anti-rabbit antibody (Invitrogen, USA), respectively ...
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bioRxiv - Biochemistry 2019Quote: ... ECL reagent (32132) and antibodies anti-oligomer A11 rabbit polyclonal (AHB0052) and goat anti-rabbit HRP conjugated (A16110) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... pneumoniae TIGR4 was detected using 1:2 optimized rabbit type-4 antiserum and 1:100 anti-Rabbit-Alexa-Fluor-488 (Thermo Fisher). Binding of human FH (Merck Millipore ...
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Direct intracellular visualization of Ebola virus-receptor interaction by in situ proximity ligationbioRxiv - Microbiology 2020Quote: ... NPC1-eBFP2 was detected using a rabbit anti-BFP antibody followed by a secondary anti-rabbit antibody-Alexa 405 fluorophore conjugate (Thermo Fisher). Cells were examined by immunofluorescence analysis as described under the method section ‘Immunofluorescence microscopy and immunostaining’.
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bioRxiv - Cell Biology 2021Quote: ... Alexa Fluor488-labeled donkey anti-rabbit (A-21206), Alexa Fluor568-647- and 680-labeled goat anti-rabbit antibodies (A-11036, A-21245, A-21109) (ThermoFisher Scientific) as well as DyLight800-conjugated goat anti-rabbit and anti-mouse antibodies (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Single immunofluorescence labeling of recombinant condensin I complex was processed as above except with 1 µg/mL rabbit anti-mSMC4 antibody (Lee et al., 2011) and Alexa Fluor 568-conjugated anti-rabbit IgG (1:500; Life Technologies) as previously described (Kinoshita et al. ...
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bioRxiv - Molecular Biology 2019Quote: ... Alexa Fluor 488 Goat anti-rabbit (A-11008) and Alexa Fluor 568 Goat anti-rabbit (A-11011) were purchased from Thermo Fisher Scientific while secondary antibodies Goat anti-mouse HRP (172-1011 ...
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bioRxiv - Immunology 2020Quote: ... gondii PVM was stained with polyclonal rabbit anti-GRA7 primary antibody (gift from John Boothroyd, Stanford University) and Alexa Fluor 488 anti-rabbit IgG (Life Technologies) at 1:3000 dilution ...
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bioRxiv - Microbiology 2021Quote: ... or rabbit TgALD1 antibody (1:200, kind gift from Dr. Kentaro Kato) and anti-rabbit HRP antibodies (Thermo Fisher, 1:10000) followed by development of signal with West Pico Plus Chemiluminescent Substrate (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2020Quote: The rabbit α-NOCT polyclonal antibody was produced in rabbits inoculated with MBP-NOCT (1-431) (Thermo Fisher custom antibody services). This MBP-NOCT antigen (1-431 ...
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bioRxiv - Cell Biology 2020Quote: ... Coverslips were then washed three times with PBS and incubated with AlexaFluor488 goat anti-rabbit or AlexaFluor647 goat anti-rabbit and AlexaFluor568 phalloidin (1:200; Life Technologies) diluted 1:200 in PBS for 1 hour at 37°C ...
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bioRxiv - Developmental Biology 2021Quote: Primary antibodies were rabbit anti-eIF4E-5 (1:500; #4524, this work) or rabbit anti-FLAG (1:500; Invitrogen, PA1-984B), and secondary antibodies were Alexa Fluor 488-conjugated anti-rabbit (1:200 ...
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bioRxiv - Microbiology 2021Quote: Viral N protein was detected using a rabbit anti-SARS-CoV N antibody [32] as a primary antibody with AlexaFluor 568 anti-rabbit IgG or anti-rabbit IgG-HRP (Thermo Fisher) as secondary antibodies together with DAPI to stain the nucleus by indirect immunofluorescence as described previously [33].
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bioRxiv - Immunology 2020Quote: ... PBMCs were fixed at 48hpi and stained intracellularly using CHIKV E1-specific rabbit antibody (kindly gifted by dr. G. Pijlman from Wageningen University) and secondary chicken anti-rabbit AF647 (Life Technologies). Samples were measured on Aurora flow cytometer (Cytek®) ...
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bioRxiv - Cell Biology 2022Quote: ... mouse anti-claudin-4 mAb (#32-9400), rabbit anti-tricellulin mAb (clone 54H19L38, #700191) and rabbit anti-ZO-2 pAb (#71-1400) were purchased from Thermo Fisher Scientific (MA ...
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bioRxiv - Neuroscience 2022Quote: ... we confirmed targeting of the optic-fiber-tetrode bundle to LC by colocalization of the electrolytic lesion with immunostaining against tyrosine hydroxylase (rabbit anti-TH, ab152, Millipore, followed by donkey anti-rabbit Alexa 488, Life Technologies, or goat anti-rabbit Alexa 594 ...
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bioRxiv - Neuroscience 2022Quote: ... matched sections were labelled in parallel with rabbit anti-μOR (1:1000, immunoStar) followed by donkey anti-rabbit Alexa Fluor 647 (1:500, Thermo Fisher). Image data were acquired using a 20X objective and z-stack tile approach on a Zeiss LSM700 confocal microscope ...
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bioRxiv - Neuroscience 2024Quote: ... 1:750, 226004, Synaptic Systems; rabbit anti-PV, 1:1000, ab11427, ITK; or rabbit anti-HDAC2, 3 µg/µL, AB_2533908, Thermo Fisher) in PBS-BT for 18 hours at RT ...
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bioRxiv - Neuroscience 2024Quote: ... A subset of sections was stained for calbindin+ cells (1⁰ rabbit anti-calbindin (Swant #CB38) 1:500 + 2⁰ anti-rabbit-633 (Invitrogen #A21071) 1:500) ...
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bioRxiv - Microbiology 2024Quote: ... respectively as described above followed by incubation with a goat anti-rabbit fluorescent secondary antibody (Invitrogen Goat anti-Rabbit Alexa Fluor Plus 568™, Cat# A-11036, 1:1000 or Invitrogen Goat anti-Rabbit Alexa Fluor™ Plus 647 ...
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bioRxiv - Microbiology 2022Quote: ... rabbit mAb (#4394); IKKβ (D30C6) rabbit mAb (#8943); phospho-IKKα (Ser176)/IKKβ (Ser177) (C84E11) rabbit mAb (#2078; blocked with SuperBlock (Thermo Scientific)) ...
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bioRxiv - Microbiology 2022Quote: ... NF-kB2 p100/p52 (D7A9K) rabbit mAb (#37359); phospho-NF-κB2 p100 (Ser866/870) rabbit mAb (#4810; blocked with SuperBlock (Thermo Scientific)) ...
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bioRxiv - Immunology 2023Quote: ... or with rabbit-anti-human TG3 (PA596450, Thermo Fischer Scientific) followed by detection with Alexa 488 donkey anti-rabbit (Molecular Probes) antibody ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The secondary antibodies were diluted to 1:250 (Goat anti-rabbit IgG Cy5; Invitrogen A10523; goat anti-mouse IgG AlexaFluor 488, Invitrogen A11029; goat anti-rabbit IgG AlexaFluor 488, Invitrogen A11034). Nuclei were stained with Hoechst 33258 (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... were selected and processed as described above with primary antibody Rabbit anti-Cox-2 (1:1k, Cayman Chem, 160123) and secondary antibody AF555 donkey anti-rabbit (1:200, ThermoFisher, A31572).
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bioRxiv - Neuroscience 2024Quote: ... Alexa Fluor 568 donkey anti-rabbit IgG or Alexa Fluor 647 donkey anti-rabbit IgG (all 1:500, Thermo Fisher Scientific) for 2 h ...
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bioRxiv - Neuroscience 2024Quote: ... and then incubated for 2 h with Alexa Fluor 488 donkey anti-rabbit IgG or Alexa Fluor 568 donkey anti-rabbit (ThermoFisher Scientific).
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bioRxiv - Neuroscience 2024Quote: ... visualized with AF488-conjugated anti-rabbit secondary antibody (1:300 from stock; Goat anti rabbit AF488, Thermo Fisher Scientific A11034, RRID:AB_2576217). Slides were imaged on an ANDOR Dragonfly microscope (Oxford Instruments ...
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bioRxiv - Cancer Biology 2021Quote: ... and protein lysates were subjected to electrophoresis through SDS–PAGE or 4-12% NuPage Bis-Tris protein gels (Thermo Fisher Scientific) followed by blotting onto nitrocellulose membranes ...
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bioRxiv - Molecular Biology 2020Quote: ... Then proteins were separated by electrophoresis in 4%–12% Bis-Tris Protein Gels and transferred to polyvinylidene difluoride membrane (Thermo Scientific). The membranes were blocked in 5% dry milk in 0.1% Tween-20 in PBS and detected with the indicated antibodies ...
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bioRxiv - Cell Biology 2020Quote: Protein concentration of cell lysates was determined using the Pierce™ BCA protein assay kit according to manufacturer’s instructions (ThermoFisher Scientific). Samples were subsequently subjected to SDS-PAGE and blotted on a nitrocellulose membrane ...
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bioRxiv - Cell Biology 2020Quote: ... forebrain samples were homogenized with lysis buffer and protein concentrations determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Equal amounts of total protein were incubated with TX antibodies (generated in our lab[23] ...
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bioRxiv - Cell Biology 2020Quote: ... and 10 µg of total protein was loaded into NuPAGE™ 4-12% Bis-Tris Protein Gels (Thermo Fisher Scientific, #NP0321BOX). Proteins were transferred to a nitrocellulose membrane using the Trans-Blot® Turbo™ RTA Mini Nitrocellulose Transfer Kit and Trans-Blot© Turbo™ Transfer System (Bio-Rad) ...
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bioRxiv - Cancer Biology 2020Quote: ... The supernatant was stored at −20°C for protein quantification performed using Pierce ™ BCA Protein Assay Kit (Thermo Fisher, 23227).
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bioRxiv - Cell Biology 2020Quote: ... the sample was recovered from the Amicon tube into a new microfuge tube and protein concentration was determined using bicinchoninic acid (BCA) protein assay (Thermo Scientific). Proteins were subjected to overnight digestion with mass spec grade Trypsin/Lys-C mix (1:25 enzyme/substrate ratio) ...