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Citations for Thermo Fisher :
8501 - 8550 of 10000+ citations for Recombinant Human PDCD1 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Post-stroke rapamycin treatment improves post-recanalization cerebral blood flow and outcome in ratsbioRxiv - Neuroscience 2023Quote: ... Protein quantification was performed using the BCA assay (Pierce BCA Protein Assay Kit, 23225, Thermo Fisher Scientific), 50 µg of protein was denatured (95°C for 5 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... The total protein amount was quantified for all collected fractions by BCA Protein Assay (PierceR, ThermoFisher scientific) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentration was determined by using the PierceTM BCA Protein Assay Kit (Cat.No.23225; Thermo Fisher Scientific). Protein extracts (60 μg ...
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bioRxiv - Genomics 2023Quote: Quantitative measurement of water-soluble protein performed using Pierce BCA Protein Assay Kit (Thermo Scientific, Rockford, IL) [26] ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were then quantified using Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, cat. no 23225), according to the manufacturer’s instructions ...
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Entorhinal cortex vulnerability to human APP expression promotes hyperexcitability and tau pathologybioRxiv - Neuroscience 2024Quote: ... Protein concentration was determined by BCA assay using Pierce™ BCA Protein Assay Kit (23225, Thermofisher scientific). For WB analyses ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentrations of lysates were determined by BCA assay (Pierce BCA Protein Assay Kit, Thermofisher Ref# 23225), equilibrated ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentrations in the supernatants were determined using the Micro BCA Protein Assay Kit (Thermo Fisher Scientific). Proteins were separated by SDS-PAGE and transferred to nitrocellulose membranes by semidry-blotting ...
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bioRxiv - Biochemistry 2024Quote: ... Antibody-bound protein was mixed with equilibrated resin from 25 µL protein A-conjugated dynabeads (Invitrogen, 1011D) for 1 hour at 4°C rotating ...
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bioRxiv - Biochemistry 2024Quote: ... The protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Cat#23225).
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bioRxiv - Immunology 2024Quote: ... The protein concentration was determined in the samples using Pierce BCA Protein Kit (Thermo Fisher Scientific Inc) and 20 μg of protein from each sample were used for Western blot.
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bioRxiv - Neuroscience 2024Quote: ... Total protein concentrations were determined using the Pierce BCA Protein Assay Kit (Thermo Fisher, Waltham, MA, USA) and stored at -80°C for future analyses ...
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bioRxiv - Neuroscience 2024Quote: ... The protein concentration was estimated using the EZQ™ protein quantitation kit (Life Technologies, Eugene, OR, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentrations were measured in the cleared lysates using the Pierce BCA Protein Assay Kit (Thermo Fisher). Then ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration from the supernatant was measured using Pierce BCA Protein Assay Kit (ThermoFisher Scientific, Cat#23225). Samples were then denatured at 950C and resolved on an SDS-PAGE gel ...
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bioRxiv - Biophysics 2024Quote: ... BlueEasy Prestained Protein Marker (Nippon Genetics Co.) and Pierce™ Unstained Protein MW Marker (Thermo Fisher Scientific) were used for SDS-PAGE analysis of CARP expression and purification ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentration was determined with the Pierce BCA Protein Assay Kit (#23225, ThermoFisher Scientific, Waltham, Ma, USA). Equal amounts of total proteins (10 μg ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentrations in cell lysates were normalized using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... The protein concentration was established with the BCA Protein Assay kit (#23227, Thermo Fisher Scientific, Rockford, USA).
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bioRxiv - Immunology 2024Quote: ... Total protein concentrations were determined using a Pierce™ BCA protein assay kit (Thermofisher Scientific, Cat # 23225). An equal amount of protein was loaded for SDS-PAGE on 10% polyacrylamide gels ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein concentration of cell lysates was also determined using a BCA protein assay kit (Thermo Scientific) and data presented depicts caspase-3/7 activity as relative fluorescence unit (RFU ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentration was determined using a colorimetric Pierce™ BSA Protein Assay Kit (#23225, Thermo Fisher Scientific). Lysates were subsequently stored at -80°C prior to experimentation.
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bioRxiv - Genetics 2024Quote: ... Precipitation of the protein-antibody conjugate was performed with DynabeadsTM Protein G (10003D, Thermo Fisher Scientific™). Chromatin-bead complexes were washed three times with Low-salt buffer followed by one wash with 1 ml High-salt buffer and eluted in TES buffer (50 mM TRIS-HCl pH 8 ...
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ATP-dependent citrate lyase Drives Left Ventricular Dysfunction by Metabolic Remodeling of the HeartbioRxiv - Molecular Biology 2024Quote: ... Proteins were separated on 4-12% SDS-PAGE gels (Precast Protein gels, Cat#NP0322BOX, Invitrogen, Waltham, MA), transferred to PVDF membranes and probed with antibodies from Cell Signaling Technology (CS ...
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bioRxiv - Neuroscience 2024Quote: ... The protein concentration was calculated using the BCA Protein Assay Reagent (23225, Pierce™ Thermo Fisher Scientific). Protein samples were separated by SDS-PAGE (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... Total protein amounts were quantified using the Pierce® BCA Protein Assay Kit (ThermoFisher Scientific, Waltham, MA). A total of 80 µg of protein homogenate were separated by SDS-PAGE and transferred onto PVDF membranes (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration was determined through the Pierce BCA Protein Assay Kit following the manufacturer’s instructions (Thermo Scientific) and 25 μg of each sample was prepared for SDS-PAGE ...
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bioRxiv - Immunology 2024Quote: ... Protein concentrations from individual samples were quantified using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) and luciferase values were normalized to protein.
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bioRxiv - Neuroscience 2024Quote: ... Protein concentrations in each sample were then determined using the BCA Protein Assay Kit (Thermo Fisher Scientific). Equal amounts of cell lysates (30 µg ...
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bioRxiv - Plant Biology 2024Quote: ... Total protein content was quantified using the Pierce™ 660nm Protein Assay Kit (©Thermo Scientific™). Following quantification ...
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bioRxiv - Bioengineering 2021Quote: ... THP-1 cells were expanded in RPMI medium (ATCC® 30-2001™) supplemented with 10% heat inactivated fetal bovine serum (HI-FBS, Gibco, ThermoFisher Scientific, Massachusetts, USA), 100 U/ml penicillin and 100 μg/ml streptomycin sulfate (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... Standard Western blotting procedures were used with the following antibodies: HIV-1 gp120 (NIH AIDS Reagent program catalog no. 288) and 6x-His Tag (ThermoFisher catalog no. MA1-21315-HRP).
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bioRxiv - Immunology 2021Quote: ... with an N-terminal IL-2 signal peptide for secretion and a C-terminal 6×His tag for purification was inserted into pFUSE-vector (Invitrogen, Thermo Fischer Scientific, MA, USA). The human ACE2 was expressed by essentially the same protocol used for the COVID-19 RBD ...
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bioRxiv - Immunology 2021Quote: ... and SARS-CoV-2 Spike RBD, His, Avitag™ (ACRO Biosystems, SPD-C82E9) immobilized on paramagnetic beads (Dynabeads M-280 streptavidin; Invitrogen Dynal AS, Oslo, Norway) as target antigen ...
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bioRxiv - Cancer Biology 2023Quote: ... we used the modified pSec-NtermHis6 vector containing secretory signal peptide (IgK Leader) as found originally in commercial vector pSecTag/FRT/V5- His-TOPOR vector (Thermo Fisher Scientific, Waltham, MA, USA), but fused to six histidine residues (His6 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were washed once with 2% HI-FBS S-MEM and further stained with PE-conjugated anti-mouse IgM (Thermo Fisher Scientific, #12-5790-81) for 30 minutes to detect the HTII-280 primary antibody ...
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bioRxiv - Microbiology 2020Quote: Toxicity of the surfaces was tested on human immortalized HaCaT keratinocytes (ThermoFisher). Cells were maintained in DMEM high glucose medium supplemented with Na-pyruvate (1mM) ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293T (Human Embryonic Kidney cells 293) cells were obtained from Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... 293T and human secondary fibroblasts cells were cultured in DMEM (Gibco, 11995) supplemented with 10% heat-inactivated FBS (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... in tissue culture plates coated with 2.5μg/cm2 human fibronectin (Thermo Fisher). HEK293T cells and L cells were cultured in DMEM with GlutaMAX supplemented with 10% fetal bovine serum (Omega Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... HCT116 cells (human colorectal epithelial) were maintained using DMEM (Gibco, 41966-029) supplemented with 10% FBS (Sigma ...
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bioRxiv - Immunology 2021Quote: Human interleukin two (IL-2) Ready-SET Go! ELISA kit (eBioscience/Invitrogen) and Nunc MaxiSorp 96-well plates (Thermo Fisher ...
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bioRxiv - Cell Biology 2020Quote: ... Human iPS WT-11 cells were cultured on Vitronectin (Thermo Fisher Scientific) coated 6-well plates or glass coverslips (for smRNA FISH purposes ...
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bioRxiv - Immunology 2021Quote: ... and Pacific Blue-conjugated mouse anti-human CD274 (ThermoFisher Scientific, MA, USA) mAbs ...
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bioRxiv - Developmental Biology 2020Quote: ... and then incubated in goat anti-human HRP secondary antibody (ThermoFisher Scientific) at 1:10,000 dilution in blocking buffer for 45-60 min at RT ...
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bioRxiv - Immunology 2022Quote: ... and against human CXCL4L1 (rabbit anti-CXCL4L1, PA5-21944, Invitrogen; 1:50). Samples were then prepared for microscopy using Duolink® mounting medium with DAPI ...
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bioRxiv - Biochemistry 2022Quote: ... 100 units/ml human LIF supplemented with 10% fetal bovine serum (Gibco). Cultures were grown in a humidified incubator at 37°C and 5% CO2.
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bioRxiv - Developmental Biology 2022Quote: ... Labelled DNA was then hybridized to Clariom S human GeneChips (ThermoFisher Scientific) and incubated at 45℃ in the GeneChip Hybridization oven 640 (Affymetrix ...
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bioRxiv - Genomics 2019Quote: ... The labeled DNA was then hybridized to Human Gene ST1.0 microarrays (Affymetrix), washed ...
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bioRxiv - Genomics 2020Quote: ... human Malat1 (positive control, Type 1 Probe, VA1-11317, Thermo Fisher Scientific), and bacterial DapB (negative control ...