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Citations for Thermo Fisher :
8451 - 8500 of 10000+ citations for Recombinant Human PDCD1 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Concentrations were normalized to total protein determined from a Pierce BCA protein assay kit (Thermo Scientific 23227).
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bioRxiv - Plant Biology 2024Quote: The total protein content of samples collected daily was determined using BCA Protein Assay Kit (ThermoFisher, US) with the standardized protocol ...
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bioRxiv - Genetics 2024Quote: ... Total protein dosage was further carried out using a BCA protein quantification kit (Life Technologies, California, USA). Western blotting protocol was performed as previously described ...
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bioRxiv - Microbiology 2023Quote: ... The amount of protein in the lysate was quantified using PierceTM BCA Protein Assay Kit (ThermoFisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... and the final protein concentration was determined using Qubit protein assay kit (#Q33211 Life Technologies Corporation, USA).
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bioRxiv - Immunology 2024Quote: ... Total protein amounts were quantified using the Pierce® BCA Protein Assay Kit (ThermoFisher Scientific, Waltham, MA). A total of 80 µg of protein homogenate were separated by SDS-PAGE and transferred onto PVDF membranes (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentrations in each sample were then determined using the BCA Protein Assay Kit (Thermo Fisher Scientific). Equal amounts of cell lysates (30 µg ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentration was determined using a colorimetric Pierce™ BSA Protein Assay Kit (#23225, Thermo Fisher Scientific). Lysates were subsequently stored at -80°C prior to experimentation.
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bioRxiv - Biophysics 2024Quote: ... BlueEasy Prestained Protein Marker (Nippon Genetics Co.) and Pierce™ Unstained Protein MW Marker (Thermo Fisher Scientific) were used for SDS-PAGE analysis of CARP expression and purification ...
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bioRxiv - Immunology 2024Quote: ... The protein concentration was determined in the samples using Pierce BCA Protein Kit (Thermo Fisher Scientific Inc) and 20 μg of protein from each sample were used for Western blot.
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bioRxiv - Cell Biology 2024Quote: ... Protein concentrations of lysates were determined by BCA assay (Pierce BCA Protein Assay Kit, Thermofisher Ref# 23225), equilibrated ...
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Entorhinal cortex vulnerability to human APP expression promotes hyperexcitability and tau pathologybioRxiv - Neuroscience 2024Quote: ... Protein concentration was determined by BCA assay using Pierce™ BCA Protein Assay Kit (23225, Thermofisher scientific). For WB analyses ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentrations in the supernatants were determined using the Micro BCA Protein Assay Kit (Thermo Fisher Scientific). Proteins were separated by SDS-PAGE and transferred to nitrocellulose membranes by semidry-blotting ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentration was determined with the Pierce BCA Protein Assay Kit (#23225, ThermoFisher Scientific, Waltham, Ma, USA). Equal amounts of total proteins (10 μg ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentrations were measured in the cleared lysates using the Pierce BCA Protein Assay Kit (Thermo Fisher). Then ...
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bioRxiv - Neuroscience 2024Quote: ... The protein concentration was estimated using the EZQ™ protein quantitation kit (Life Technologies, Eugene, OR, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Total protein concentrations were determined using the Pierce BCA Protein Assay Kit (Thermo Fisher, Waltham, MA, USA) and stored at -80°C for future analyses ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration from the supernatant was measured using Pierce BCA Protein Assay Kit (ThermoFisher Scientific, Cat#23225). Samples were then denatured at 950C and resolved on an SDS-PAGE gel ...
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bioRxiv - Biochemistry 2024Quote: ... Antibody-bound protein was mixed with equilibrated resin from 25 µL protein A-conjugated dynabeads (Invitrogen, 1011D) for 1 hour at 4°C rotating ...
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bioRxiv - Biochemistry 2024Quote: ... The protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Cat#23225).
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bioRxiv - Biochemistry 2024Quote: ... Protein concentrations in cell lysates were normalized using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... Precipitation of the protein-antibody conjugate was performed with DynabeadsTM Protein G (10003D, Thermo Fisher Scientific™). Chromatin-bead complexes were washed three times with Low-salt buffer followed by one wash with 1 ml High-salt buffer and eluted in TES buffer (50 mM TRIS-HCl pH 8 ...
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bioRxiv - Neuroscience 2024Quote: ... The protein concentration was established with the BCA Protein Assay kit (#23227, Thermo Fisher Scientific, Rockford, USA).
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bioRxiv - Neuroscience 2024Quote: ... The protein concentration was calculated using the BCA Protein Assay Reagent (23225, Pierce™ Thermo Fisher Scientific). Protein samples were separated by SDS-PAGE (Thermo Fisher Scientific ...
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ATP-dependent citrate lyase Drives Left Ventricular Dysfunction by Metabolic Remodeling of the HeartbioRxiv - Molecular Biology 2024Quote: ... Proteins were separated on 4-12% SDS-PAGE gels (Precast Protein gels, Cat#NP0322BOX, Invitrogen, Waltham, MA), transferred to PVDF membranes and probed with antibodies from Cell Signaling Technology (CS ...
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bioRxiv - Immunology 2024Quote: ... Total protein concentrations were determined using a Pierce™ BCA protein assay kit (Thermofisher Scientific, Cat # 23225). An equal amount of protein was loaded for SDS-PAGE on 10% polyacrylamide gels ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein concentration of cell lysates was also determined using a BCA protein assay kit (Thermo Scientific) and data presented depicts caspase-3/7 activity as relative fluorescence unit (RFU ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration was determined through the Pierce BCA Protein Assay Kit following the manufacturer’s instructions (Thermo Scientific) and 25 μg of each sample was prepared for SDS-PAGE ...
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bioRxiv - Immunology 2024Quote: ... Protein concentrations from individual samples were quantified using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) and luciferase values were normalized to protein.
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bioRxiv - Plant Biology 2024Quote: ... Total protein content was quantified using the Pierce™ 660nm Protein Assay Kit (©Thermo Scientific™). Following quantification ...
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bioRxiv - Biophysics 2020Quote: The pFAST-bac1vectors were used to produce recombinant baculovirus according to the protocol for the Bac-to-Bac system (Life Technologies). Recombinant GST-A3H Hap I WT and mutant baculovirus (K117E ...
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bioRxiv - Genetics 2021Quote: ... using 2 µg of total RNA in the presence of 2 U/µl of RNaseOut Recombinant RNase Inhibitor (Thermo Fisher Scientific). Genomic DNA was eliminated using the RapidOut DNA Removal Kit (Thermo Fisher Scientific) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Recombinant bacmids were made according to instructions for the Bac-to-Bac® Baculovirus expression system given by the manufacturer (Invitrogen) using DH10EMBacY (Geneva Biotech) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Plasmid DNAs were isolated according to standard protocols and recombinant plasmids were subjected to sequencing using universal M13 primers and the BigDye terminator cycle sequencing kit v1.1 (Thermo Fisher Scientific). Sequencing products were EDTA/ethanol-precipitated ...
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bioRxiv - Developmental Biology 2021Quote: ... Recombinant lentivirus was used to infect NIH3T3 cells and stably expressing cells selected with 5 μg/ml puromycin (Thermo Fisher, A1113803). Conditional expression of CB1R was induced by the addition of Doxycycline (Millipore Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... and further digested for 5 min at room temperature (22°C) using a TrypLE Express recombinant protease (ThermoFisher Scientific, cat. #12605010). The dissociated cells were sedimented by centrifugation (900 g for 10 min at 4°C) ...
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bioRxiv - Developmental Biology 2019Quote: ... Recombinant HSF2 (TP310751 Origen) was desalted (ZebaTM Spin Desalting Columns, 7K molecular-weight cutoff, 0.5 ml (1034–1164, Fisher Scientific, Germany)) and biotinylated at a molar ratio biotin/protein (3:1 ...
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bioRxiv - Cell Biology 2019Quote: ... sequence-verified plasmids (wild-type and mutant variants of pbiG2ab:6His-ZZ-SNAPf-DHC1/IC2/LIC2/Tctex1/Robl1/LC8) were used to generate recombinant baculoviral genomes by Tn7 transposition into DH10Bac cells (Life Technologies). White ...
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bioRxiv - Developmental Biology 2019Quote: ... was introduced into the BglII and SpeI sites of pCR4-HBB-24XMS2SL and the recombinant HBB- 24XMS2SL-MCS sequence was subcloned into the pcDNA3 mammalian expression vector (ThermoFisher Scientific) using the NotI and XbaI sites ...
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bioRxiv - Biochemistry 2021Quote: Recombinant baculoviruses for expression of the mTTYH3 ECD1-T4L-ECD2 construct were generated using the Bac-to-Bac system (ThermoFisher Scientific). Sf9 cells were cultured at 27 °C in 1 L culture flasks with ESF-921 insect cell culture medium (Expression systems) ...
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bioRxiv - Immunology 2021Quote: CASP1-/- and NLRP1-/- N/TERT-1 keratinocyte cells were prepared by delivery of Cas9 ribonucleoprotein complexes containing an Alt-R CRISPR-Cas9 sgRNA and recombinant Cas9 (IDT) using the Neon Transfection System (ThermoFisher Scientific) following the manufacturer’s recommendations ...
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bioRxiv - Bioengineering 2020Quote: ... luminescence was compared to a ladder of recombinant Gaussia Luciferase (NanoLight #321-500) quantified using a Gaussia Luciferase Glow Assay Kit (ThermoFisher #16161) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... immunogen SARS-CoV-2 spike protein that interact with HeLa cell expressed SARS-CoV-2) and anti ACE2 antibody (ACE2 Recombinant Rabbit Monoclonal Antibody (Cat# MA532307; interact with mouse ACE2) were obtained from Life Technologies Corporation.
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bioRxiv - Cancer Biology 2020Quote: ... and 150 ng were used for the BP recombinant reaction with pDONR221 according to the Gateway technology manual (Thermo Fisher Scientific). Chemically competent E ...
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bioRxiv - Cell Biology 2021Quote: ... two and three consecutive phage enrichment rounds were performed either with recombinant Miro1 immobilized on NuncTM ImmunoTM MaxiSorpTM tubes (Thermo Scientific) or HEK293-expressed GFP-Miro1 immobilized on ChromoTek GFP- Trap® Multiwell plate (Proteintech) ...
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bioRxiv - Plant Biology 2021Quote: ... Inserts from the recombinant pENTR plasmids were transferred to destination vectors via gateway cloning by using the LR clonaseTM II Enzyme mix (Invitrogen, USA) and by following the manufactureŕs instructions ...
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bioRxiv - Genomics 2021Quote: Cryopreserved stocks of the KH004 parent and the Mal31×KH004 uncloned bulk recombinant progeny pool and were thawed and grown in complete media (CM) [RPMI 1640 with L-glutamine (Gibco, Life Technologies), 50 mg/L hypoxanthine (Calbiochem ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant baculovirus was generated in Sf9 cells using the Bac-to-Bac system according to the manufacturer’s instructions (Invitrogen, Carlsbad, CA). Recombinant proteins were then expressed by infecting Hi5 cells for 96 h at 28°C ...
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bioRxiv - Cell Biology 2021Quote: ... or HIF1β were expressed in Sf9 cells that had been infected with recombinant baculovirus as described in the Bac-to-Bac® Baculovirus Expression System from Invitrogen.
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bioRxiv - Molecular Biology 2022Quote: ... intracellular ATP levels were determined by reaction of ATP with recombinant firefly luciferase and its substrate D-luciferin according to the manufacturer’s instructions (Thermo Scientific, A22066).