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Citations for Thermo Fisher :
8501 - 8550 of 10000+ citations for 2 3 5 6 Tetrahydroxy 4 phosphonooxycyclohexyl dihydrogen phosphate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... and the homozygote variant iPSCs were grown in a feeder-free manner on Matrigel (Corning)-coated 6-well plates in Essential 8 (E8) medium (ThermoFisher Scientific). Media was changed daily ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were loaded onto precast 6% TBE (Invitrogen, Fisher cat #EC62655BOX) or 10% TBE-Urea (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... for detection in QuantStudio 6 Pro cycler (Applied Biosystems Carlsbad, CA). Quantification of each transcript was normalized to the mouse 18S reference gene following the 2-ΔΔCt method ((Livak & Schmittgen ...
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bioRxiv - Cancer Biology 2024Quote: ... Genomic DNA was eluted 6 times in ultrapure water (Thermofisher, 10977) for maximum recovery ...
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bioRxiv - Neuroscience 2024Quote: ... were coated with IL-6 capture antibody (ThermoFisher Scientific, MP5-20F3) and left overnight at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... and eluted in 6 μl nuclease-free water (Ambion, cat# AM9937).
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bioRxiv - Molecular Biology 2024Quote: ... Samples were loaded onto 6% TBE-Urea gel (EC6865BOX, Thermo Fisher) and cDNAs exceeding 200 nt were excised from the gel and extracted using Crush-Soak Gel buffer (as per iiCLIP protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Some PCR products were run on 6% TBE gels (Invitrogen, #EC6865BOX) which were then stained with SYBR-Gold DNA stain (Invitrogen ...
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Langerhans cells regulate immunity in adulthood by regulating postnatal dermal lymphatic developmentbioRxiv - Immunology 2024Quote: ... on a Applied BiosystemsTM QuantStudio 6 Pro (Thermo Fisher Scientific, #A43180). Transcript levels were calculated relative to controls and the relative fold change was calculated using the 2−ΔΔCt algorithm ...
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bioRxiv - Immunology 2024Quote: ... and tibia from male C57BL/6 mice with RPMI 1640 (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... and QuantStudio 6 Flex real-time PCR System (Thermo Fisher Scientific). An arbitrary standard curve was used to determine relative quantities of DNA ...
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bioRxiv - Immunology 2024Quote: ... and Eotaxin were determined by 6-plex magnetic bead panel (Invitrogen) following the manufacturer’s instructions with appropriate dilution ...
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bioRxiv - Immunology 2024Quote: ... Cells were plated at 5x106/mL into 6-well plates (ThermoFisher) and cultured at 37°C with 5% CO2 incubator and fed with fresh Flt3L-containing medium every 3 days until maturity at 10 days ...
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bioRxiv - Immunology 2024Quote: ... 6% v/v DMSO (cat. no. D12345; Invitrogen, Waltham, MA, USA), 54% v/v sterile normal saline ...
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bioRxiv - Neuroscience 2024Quote: ... USA)-coated 6-well plates in Essential 8TM Medium (Thermo Scientific, USA). Rock inhibitor (10 μM ...
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bioRxiv - Physiology 2024Quote: ... on a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) with specific primers for each gene ...
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bioRxiv - Neuroscience 2024Quote: ... or Quant Studio 6 Pro Real-Time PCR System (Applied Biosystems). TaqMan assay details are listed in Tab ...
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bioRxiv - Biochemistry 2024Quote: ... and 0.30 M di-benzo-18-crown-6-ether (Thermo Scientific) following published protocols66,67,79,80,110 ...
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bioRxiv - Cancer Biology 2024Quote: ... The desired DNA product was purified with 6% TBE gel (Invitrogen) and samples were sequenced on an Illumina HiSeq2000 ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were grown for 6 passages in DMEM-HAM’s F12 (Gibco) supplemented with 5% (v/v ...
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bioRxiv - Microbiology 2024Quote: ... Real-time qPCR was performed on QuantStudio 6 Pro (Applied Biosystems) using iTaq Universal SYBR Green Supermix (Biorad) ...
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bioRxiv - Immunology 2024Quote: ... and human IL-6 (Gibco, PeproTech, #200-06; 20 ng/mL). Prostaglandin E2 (PGE2 ...
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bioRxiv - Immunology 2024Quote: ... and real-time PCR system (Applied Biosystems 7500 QuantStudio 6 Pro). The Ct value of target genes obtained from each sample was normalized to housekeeping gene GAPDH and relative gene expression was quantified using 2-ΔΔct values.
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bioRxiv - Genetics 2023Quote: ... and analysed using the Quant Studio 6 Flex system (Applied Biosystems). The real-time qPCR conditions were one hold at (95 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were centrifuged using a Fiberlite F9-6×1000 (Thermo Fisher) at 4 °C and 2,500 xg ...
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bioRxiv - Neuroscience 2024Quote: ... 6 cm Nunc EasYDish dishes (catalog number 150462, Thermo Fisher Scientific) were coated overnight before the harvest with 3 mL of 50 μg/mL poly-D-lysine hydrobromide (catalog number P6407 ...
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bioRxiv - Immunology 2024Quote: ... on a QuantStudio 6 Flex RT-PCR system (Thermo Fisher Scientific) with the QuantStudio™ Real-Time PCR Software v1.7.2 ...
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bioRxiv - Neuroscience 2024Quote: ... diluted 6-fold in Opti-MEM™ I (Thermo Fisher, 31985062). Cells were cultured in DMEM/F12 GlutaMax with 10% FBS ...
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bioRxiv - Cell Biology 2024Quote: ... were grown in a feeder-free manner on Matrigel (Corning)-coated 6-well plates in Essential 8 (E8) medium (ThermoFisher Scientific). Media was changed daily ...
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bioRxiv - Bioengineering 2024Quote: ... Immediately after the addition of 6 μL DEPC water (Fisher Scientific), the column was centrifuged at 16,000 ×g for 2 min ...
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bioRxiv - Cancer Biology 2024Quote: ... on a QuantStudio 6 Flex Real Time PCR System (Applied Biosystems). Expression data were analyzed using the ΔΔCt method against β-actin (housekeeping control ...
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bioRxiv - Cell Biology 2024Quote: ... on the QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems). All qPCR experiments were conducted with at least three biological replicates ...
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bioRxiv - Cell Biology 2024Quote: ... they were cultured in Essential 6 medium (Thermo Fisher Scientific, A1516401) with SB-431542 (10 µM ...
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bioRxiv - Cell Biology 2024Quote: ... A QuantStudio 6 Flex Real-time PCR System (Thermo Fisher Scientific) was used to conduct polymerase chain reaction (PCR) ...
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bioRxiv - Cell Biology 2024Quote: ... 6-((acryloyl)amino)hexanoic acid (Acryloyl-X, SE, A-20770, ThermoFisher); Sodium acrylate (408220 ...
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bioRxiv - Cell Biology 2020Quote: ... 2 μM Rhod-2 AM (Thermo, R1244) and 2 μM Calcium green-1 AM (Life Technologies, C3011MP) was added to culture media and allowed to stain at 22 °C for 20 min prior to imaging (frozen Rhod-2 AM aliquots were used only when the DMSO suspension remained clear) ...
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bioRxiv - Immunology 2021Quote: ... Wells were washed five times with PBS-Tween 20 and Pierce p-Nitrophenyl Phosphate (PNPP) (Thermo Scientific, Cat. #37620) was added to each well to develop plates for 30 minutes in the dark at room temperature ...
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bioRxiv - Systems Biology 2020Quote: ... HDM extract (Greer Labs; Lenoir, NC) was prepared in sterile phosphate buffered saline (pH 7.4; Life Technologies; Waltham, MA). The HDM extract we used contained 36,000 endotoxin units (EU ...
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bioRxiv - Genomics 2020Quote: ... Dechorionated embryos were then transferred to a 1.5mL microcentrifuge tube and then washed twice with 1 X PBS (Phosphate buffer solution, ThermoFisher). RLN buffer (Sigma ...
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bioRxiv - Immunology 2021Quote: Paramagnetic nanoparticles (Strep Plus 300nM, Ademtech) were washed with Dulbecco’s phosphate-buffered saline with calcium and magnesium (DPBS++, ThermoFisher). The nanoparticles were resuspended in DPBS++ containing 1μM biotin-labeled anti-human kappa light chain (Igκ ...
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bioRxiv - Physiology 2020Quote: ... The cells were quickly washed with phosphate-buffered saline (PBS) followed by a change in medium to RPMI (ThermoFisher) containing 3 μM CHIR99021 (Stem Cell Technologies) ...
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bioRxiv - Synthetic Biology 2020Quote: ... the cells were diluted 1:10 (10 μL into 90 μL) in phosphate-buffered saline (PBS) (Gibco,18912-014) containing 2 mg/mL kanamycin to halt protein translation and incubated at room temperature for 1 hour to allow for maturation of the YFP before performing flow cytometry.
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bioRxiv - Genomics 2020Quote: ... and washed with cold phosphate-buffered saline (PBS) and then resuspended in lysis buffer (Dynabeads mRNA Direct kit, ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... the precipitates were resuspended in phosphate buffer (20 mM Na3PO4 pH 7.5) supplemented with protease inhibitor cocktail (Thermo Scientific) and the resuspended cells were lysed by sonication (10 cycles ...
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bioRxiv - Bioengineering 2021Quote: ... The virus was then dialyzed with 1× phosphate buffered saline (pH 7.2) supplemented with 50 mM sodium chloride and 0.0001% Pluronic F68 (Thermo Fisher) using 50 kDA filters (Millipore) ...
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bioRxiv - Bioengineering 2021Quote: ... Size-exclusion chromatography was performed using a GE Superdex 200 Increase 10/300 GL column pre-equilibrated in 1x Dulbecco’s phosphate-buffered saline (Gibco). Fractions were evaluated by SDS-PAGE (4-20% Mini-PROTEAN TGX ...
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bioRxiv - Neuroscience 2020Quote: ... cells were washed once with Dulbecco’s Phosphate-Buffered Saline with Calcium and Magnesium (Thermo-Fisher Scientific, Cambridge, MA, USA) and treated with 0.25% Trypsin-EDTA solution (Thermo-Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... solution for 15 minutes at room temperature and washed twice with 1X phosphate buffer solution (PBS; Thermo Fisher Scientific). Fixed cells were permeabilized with PBS containing 0.2% Triton X-100 (Sigma Aldrich ...
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bioRxiv - Cell Biology 2021Quote: Cryosections were rehydrated for 30 min with phosphate-buffered saline (PBS, no calcium, no magnesium, Thermo Fisher Scientific, USA). Blocking and permeabilization were performed for 1 h at RT with 10 % normal donkey serum and 0.2 % Triton-X in PBS ...
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bioRxiv - Cell Biology 2021Quote: ... The purified Fabs were is buffer exchanged against 10 mM phosphate solution buffered at pH of 6.50 and concentrated using the Speed Vacuum (SAVANT Speed Vacuum, Thermofisher) until the final concentration of 5 mg/mL ...