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Citations for Thermo Fisher :
8751 - 8800 of 10000+ citations for 2 3 5 6 Tetrahydroxy 4 phosphonooxycyclohexyl dihydrogen phosphate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... 2 (Applied Biosystems).
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bioRxiv - Molecular Biology 2024Quote: ... Agarose (Invitrogen, 2%) was dissolved in PBS to create a sterile moulding solution ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... B27 (2%, Invitrogen), GlutaMax (0.25% ...
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bioRxiv - Cancer Biology 2024Quote: ... 2% glutamine (GIBCO) and 1% penicillin/streptomycin (Sigma/Merck ...
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bioRxiv - Cell Biology 2024Quote: ... 2% B27 (Gibco), 1% PenStrep (Gibco) ...
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bioRxiv - Cancer Biology 2024Quote: ... 2% GlutaMAX (Gibco), and 1% Pen/Strep ...
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bioRxiv - Bioengineering 2024Quote: ... interleukin-2 (Gibco), or with Immunocult Human CD3/CD28 T cell activator (STEMCELL Technologies).
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bioRxiv - Developmental Biology 2024Quote: ... 2% B27 (Gibco), 1mM HEPES (ThermoFisher) ...
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bioRxiv - Developmental Biology 2024Quote: ... 2% B27 (ThermoFisher), 25ng/ml cardiotrophin (Peprotech EC) ...
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bioRxiv - Cell Biology 2020Quote: ... Endoplasmic reticulum (ER) was labeled with DiI (1,1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine perchlorate, aka DiIC18(3)) (Thermo Fisher #D282) or DiD (1,1’-dioctadecyl-3,3,3’,3’-tetramethylindodicarbocyanine perchlorate ...
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bioRxiv - Physiology 2020Quote: Caspase-3-like activity was quantified using the EnzChek Caspase-3 Assay kit #1 (Molecular Probes, Eugene, OR, USA). Tissue samples were thawed on ice for 5 min ...
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bioRxiv - Biochemistry 2020Quote: Cultivated Jurkat cells were collected and washed 3 times in flow buffer (PBS without Ca/Mg, 3% FBS, Gibco). Cell concentration was measured ...
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bioRxiv - Cancer Biology 2020Quote: ... TIC-enriching 3-D cultures (3-D) were maintained in stem cell media: DMEM:F12 (+ L-glutamine, + 15 mM HEPES) (Gibco) supplemented with 1% penicillin/streptomycin ...
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bioRxiv - Cancer Biology 2020Quote: ... Reactions were run on the QuantStudio 3 and analyzed on the QuantStudio 3 Design and Analysis software v1.5.1 (ThermoFisher Scientific). Quantitation and normalization of relative gene expression were accomplished using comparative threshold cycle method or ΔΔCT.
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bioRxiv - Cell Biology 2021Quote: Total RNA from control (n=3) and model (n=3) Huh7 cells were isolated by TRIZOL reagent (Thermo Scientific), and the RNA concentration ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then lysed and a caspase-3 assay was performed according to the manufacturer’s protocol (EnzChek caspase-3 Assay, Invitrogen). The intensity of fluorescence was analyzed using an EnVizion plate reader.
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bioRxiv - Developmental Biology 2020Quote: RNA from Rbx2 fl/fl (n=3) and Rbx2cKO-Nes (n=3) P1 telencephalons was extracted using TRIzol (Invitrogen). Strand-specific and barcode-indexed RNA-seq libraries were generated from 1 μg total RNA each after poly-A enrichment using the Kapa Stranded mRNA-seq kit (KapaBiosystems ...
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bioRxiv - Immunology 2020Quote: 3’ RACE analysis was performed on testis and liver RNA using the 3’ RACE System kit (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Detection of active caspase 3/7 was accomplished using CellEvent™ Caspase-3/7 Red Detection Reagent (ThermoFisher Scientific). Sorted CD4SP Rag1GFP+ thymocytes were stained with CD5-PerCP-Cy5.5 (53-7.3 ...
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bioRxiv - Cell Biology 2023Quote: ... for 30 min at room temperature using 10 mM Sodium Acetate [pH 5.0] buffer containing 5μg/ml 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC, Thermofisher, 22980) as coupling agent ...
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bioRxiv - Molecular Biology 2023Quote: ... with the human dystrophin 3′ UTR or mutant 3′ UTR and with 50 nM miR-146a mimic (Life Technologies) with Lipofectamine 2000 according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... 3′-ligated RNA fragments and subtracted 3′-ligated RPF fragments were reverse-transcribed using SuperScript III Reverse Transcriptase (Invitrogen) at 48 °C for 30 min ...
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bioRxiv - Physiology 2024Quote: ... 40 μl of total blood were stained with a fluorescent lipophilic dye (3, 3′-dyhexiloxacarbocyanine iodide; DiOC6, Molecular Probes) to obtain absolute counts of erythrocytes ...
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bioRxiv - Cancer Biology 2024Quote: ... was injected into buccal mucosa of tumor-bearing KOG mice 3 times every 3 days along with 100 μg polyI:C (Invitrogen) as adjuvant.
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bioRxiv - Bioengineering 2020Quote: ... Bone marrow human macrophage (KG-1 ATCC® CCL246™) passages 2–5 were cultured in cell suspension flasks using IMDM medium (Gibco™ GlutaMAX™, HEPES) with 20% FBS ...
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bioRxiv - Cell Biology 2020Quote: ... each well was washed twice in ice-cold PBS and the cells were scraped off in ice-cold PBS with added protease inhibitors (1:100 Halt, cat nb. 78429, Thermofisher; 5 mM NAM; 2 mM NaOV). The suspension was transferred to a tube and centrifuged for 10 min at 2000g at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... before they were transferred into 2° antibodies dilute (1:700 of Dylight-/Alexa-conjugated antibodies made in donkey purchased from Thermo Fisher Scientific in 5% BSA/PBST) for 2 hrs at RT ...
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bioRxiv - Plant Biology 2020Quote: ... Extracted peptides were resuspended in 20 μl of 5% [v/v] ACN and 0.1% [v/v] TFA and 5 μl of peptide solution were loaded onto a 2 cm C18 reversed phase trap column (Acclaim PepMap100, diameter: 100 μm, granulometry: 5 μm, pore size: 100 Å; Thermo Fisher Scientific, Waltham, MA, USA) to be further separated on a 50 cm C18 reversed phase analytical column (Acclaim PepMap100 ...
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bioRxiv - Immunology 2021Quote: ... or control PBS added onto a slant of 2 mL of TSA within a 5 mL round bottom polypropylene tube (Thermo Fisher Scientific Inc., Wilmington, DE, USA) (Supplemental Figure S1) ...
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bioRxiv - Plant Biology 2024Quote: ... Trapping was performed at 20 μl/min for 2 min in loading solvent A on a 5 mm trapping column (Thermo scientific, 300 μm internal diameter (I.D.), 5 μm beads) ...
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bioRxiv - Immunology 2022Quote: ... Harvested naïve CD4+ T cells were plated at a density of 1.5 × 105 cells/mL in 2 ml of complete IMDM ((IMDM [Life Technologies], 10% FBS [26140079, Life Technologies], 1% Penicillin-Streptomycin [Life Technologies], and 0.05% (50μM) 2-ME [Sigma-Aldrich]) ...
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bioRxiv - Molecular Biology 2023Quote: ... Trapping was performed at 20 μL/min for 2 min in loading solvent A on a 5 mm trapping column (Thermo scientific, 300 μm internal diameter (I.D.), 5 μm beads) ...
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bioRxiv - Evolutionary Biology 2023Quote: Samples of salmonid CF and non-salmonid OF (2 µg of proteins for each sample) were subjected to a short migration on a NuPAGE system (Invitrogen, 3 minutes at 200 V-400 mA - 23 W) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... One µg of each sample (3 biological replicates for each cell type) was loaded onto a C18 reverse-phase analytical column (Thermo scientific, EASY-Spray 50 cm, 2 µm particle size). Separation was achieved with a two-and-a-half-hour gradient method and was set to 4 - 35% buffer B (v/v ...
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bioRxiv - Molecular Biology 2022Quote: ... pLV-mCherry at ratio 1:1:1 (i.e. 5:5:5 μg) using Lipofectamin™ 3000 transfection reagent (Thermo-Fisher Scientific, #L3000015). Pseudoviruses harvested from the supernatant at 48 h 72h post-transfection were filtered (0.44 μm ...
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bioRxiv - Cell Biology 2023Quote: ... Leukocytes were washed with cold PBS followed by another centrifugation step (300 x g 5 minutes) and resuspended in 5 mL of 5 μg mL-1 of Hoechst 33342 (Thermo Fisher Scientific) diluted in warm PBS ...
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bioRxiv - Pathology 2023Quote: ... one lung section 5×5×5 mm in size was finely cut and stored at -20°C in RNAlater (Thermo Scientific, US). We mechanically disrupted the tissue from RNAlater using a MediMachine (BD Biosciences ...
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bioRxiv - Biochemistry 2024Quote: ... the RNA was subjected to 5’ adapter ligation with a 5’ chimeric DNA-RNA adapter (5’aminolinker-GTTCAGAGTTCTACAGTCCGACGATCrNrNrNrN) using RNA ligase (EL0021, Thermo Fisher Scientific) at 37°C for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: Deeply anesthetized 8-9 week old male and female C57BL/6JOlaHsd mice were perfused for 5 min with sulfo-NHS-biotin (4 mg/20 g mouse body weight, Thermo Scientific, cat no. 21217, dissolved in 20 ml PBS). Brains were dissected and handled as described under tissue preparation section.
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bioRxiv - Microbiology 2022Quote: ... interleukin-10 (IL-10) and interleukin-4 (IL-4) levels by sandwich ELISA kits (ThermoFisher Scientific). The measurement from unstimulated splenocytes (incubated with medium only ...
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bioRxiv - Immunology 2021Quote: ... 4°C) and cells were spun onto glass slides using a Cytospin 4 Cytocentrifuge (Thermo Scientific), dried for 10 min ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were migrated on precast Novex 4–20% or 4–12% polyacrylamide gels (Thermo Fisher Scientific), then transferred to Novex nitrocellulose membranes (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... and then fixed overnight at 4°C in 4% paraformaldehyde (Alfa Aesar, Thermo Fisher Scientific, UK), followed by several washes in 0.1M Phosphate buffered saline (PBS ...
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bioRxiv - Cell Biology 2021Quote: ... only 4 μL of 1 mM FM 4-64 (Cat. number T13320, Thermo Fisher, Waltham, MA) was injected ...
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bioRxiv - Cell Biology 2023Quote: ... diluted in fresh DMEM for 4 h followed by fixation with 4% PFA (Thermo Fisher Scientific). To induce expression of the hGRAD and TRIM21 systems ...
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bioRxiv - Physiology 2023Quote: ... 0.5 mg protein was precleared for 30 minutes at 4°C with 4% Agarose beads (ThermoFisher). To immunoprecipitate RYR1 ...
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bioRxiv - Biochemistry 2024Quote: ... samples were run on Bolt 4–12% SDS-polyacrylamide and native PAGE 4-16% gels (Invitrogen) respectively ...
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bioRxiv - Biochemistry 2022Quote: ... samples were run on Bolt 4–12% SDS-polyacrylamide and native-PAGE 4-16% gels (Invitrogen) respectively ...
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bioRxiv - Systems Biology 2024Quote: ... A Dionex IonPac AS11-HC-4 µm column (250 × 0.4 mm, 4 µm; Thermo Fisher Scientific) was used at 35°C to separate metabolite ...
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bioRxiv - Biochemistry 2024Quote: ... n=4 MCT-Vehicle and n=4 MCT-Fer-1 using PureLink RNA Mini kit (ThermoFisher) with DNase ...