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Citations for Thermo Fisher :
8451 - 8500 of 10000+ citations for Biotin Z Antibody Internalization Kit rabbit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... The antibody was then labeled in a 200 μL small-scale reaction using the DSB-X labeling kit (Molecular Probes) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... H06 IPSE was conjugated to Alexa Fluor 488 using a Alexa Fluor 488 antibody labeling kit (Thermofisher Scientific, Waltham, MA) according to manufacturer instructions ...
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bioRxiv - Microbiology 2020Quote: ... containing a 1/250 dilution of Alexa 488-conjugated anti-Spike antibody (GTX632604 conjugated using the Zenon Alexa Fluor 488 Mouse IgG Labeling Kit, ThermoFisher) and washed 4 times in FACS buffer ...
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bioRxiv - Cell Biology 2019Quote: ... For direct labeling of the anti-CC10 antibody Zenon Alexa Fluor 546 mouse IgG1 kit was used according to the manufacturer’s recommendations (Invitrogen, 25004). Stained cryosections were analyzed on an epifluorescence microscope (Zeiss Imager.M2 ...
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bioRxiv - Immunology 2020Quote: ... and detection antibody against mouse IgA (1 in 250) from the mouse total IgA ELISA kit from Invitrogen (CA, USA). The positive control (anti-S IgG ...
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bioRxiv - Immunology 2020Quote: ... The APC decoy reagent was generated by conjugating SA-APC to Dylight 755 using a DyLight 755 antibody labeling kit (ThermoFisher), washing and removing unbound DyLight 755 ...
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bioRxiv - Cell Biology 2021Quote: WBM cells were incubated for 30 minutes at 4°C with antibodies for HSC isolation (CD34, CD48, Sca, c-Kit; Invitrogen), lineage markers (CD3e ...
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bioRxiv - Biochemistry 2021Quote: ... the thermal unfolding temperatures of the chimeric antibodies (0.3 mg/mL) were assessed in triplicate using the Protein Thermal Shift Dye Kit (ThermoFisher Scientific). Continuous fluorescence measurements (λex = 580 nm ...
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bioRxiv - Immunology 2020Quote: ... cells were stained with extracellular antibodies followed by intracellular staining of EdU using click-iT Plus EdU Alexa Fluor 488 Flow Cytometry Assay Kit (Invitrogen), according to the manufacturer’s instructions.
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bioRxiv - Genetics 2022Quote: ... The Flow Cytometry analysis panel consisted of the following antibodies: Live/dead fixable near-IR Dead cell stain kit L34975 (Invitrogen); 421 Rat Anti-Mouse CD196 (CCR6 ...
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bioRxiv - Biochemistry 2022Quote: ... Plasmids encoding the heavy chain and light chain of antibodies were transfected into Expi293F cells using an Expifectamine 293 transfection kit (Gibco) in a 2:1 mass ratio following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Fab were generated from monoclonal antibodies using the Pierce Mouse IgG1 Fab and F(ab’)2 Preparation Kit (Thermo Fisher). Antibodies were first digested into Fab in a Zeba Desalt Spin Column (Thermo Fisher ...
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bioRxiv - Cell Biology 2022Quote: ... Levels of active Rac1 and Cdc42 were detected using Rac1 and Cdc42 specific antibodies from the active Rac1/Cdc42 pull-down kit (Cat# 16118/19, ThermoFisher). Levels of GST-Pak1-PBD protein were detected using Ponceau S staininbg of the membrane ...
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bioRxiv - Neuroscience 2024Quote: ... membranes were washed after secondary antibody incubation before protein detection using SuperSignal West Pico Chemiluminescence Detection kit (Thermo Fisher Scientific). Images were acquired with a Bio-Rad Chemidoc XRS+ gel imaging system and analyzed with ImageJ/Fiji software (National Institutes of Health) ...
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bioRxiv - Immunology 2024Quote: ... and immunoprecipitation was performed with 2μg of anti-survivin antibodies (RnD AF886) coupled to the Dynabeads Protein G Immunoprecipitation Kit (10007D, ThermoFisher Scientific), cross-linked with bis(sulfosuccinimidyl)suberate (A39266 ...
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bioRxiv - Microbiology 2024Quote: Plasmids encoding the heavy chain and light chain of the antibody were transfected at an equimolar ratio into Expi293F cells using ExpiFectamine 293 transfection kit (Gibco) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... The GDF-15 and GFP antibodies were biotinylated using an EZ-Link Micro NHS-PEG4-Biotinylation Kit from Thermo Fisher Scientific (21955 ...
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bioRxiv - Cancer Biology 2023Quote: ... The concentration of the antibody was estimated using a Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific, Waltham, MA, USA). Approximately 4 mg of antibody (2 mL of antibody ...
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bioRxiv - Genomics 2023Quote: ... and SXL (1:500, a gift from Fatima Gebauer) antibodies using the Western Breeze kit following the manufacturer’s protocol (ThermoFisher Scientific). We quantified the relative expression of protein for SXL using the gel analysis tool in ImageJ software following the website’s guidelines 90 ...
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bioRxiv - Immunology 2023Quote: ... Cells were subsequently stained with surface antibodies and then either fixed using 1% paraformaldehyde or permeabilized using a FoxP3/Transcription factor fixation/permeabilization kit (ThermoFisher). Samples undergoing permeabilization were then stained with intracellular antibodies ...
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bioRxiv - Molecular Biology 2023Quote: ... The concentrations of the conjugated oligonucleotide DNA and antibodies were quantified using a BCA Protein Assay Kit (Thermo Scientific 23225) and Nanodrop (Thermo Scientific).
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bioRxiv - Microbiology 2023Quote: ... Plasmids encoding the heavy chain and light chain of antibodies were transfected into Expi293F cells using an Expifectamine 293 transfection kit (Gibco) in a 2:1 mass ratio following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Three technical replicates of 100 µg protein from each biological replicate diluted with diluent supplied by the manufacturer were added to the antibody-coated wells (Ferritin Human ELISA Kit, Invitrogen). All the steps were carried out following the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... Antibody units were then calculated in μg/ml from the standard sera which was quantified using antibody quantification kits from Invitrogen for IgG (88–50400 ...
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bioRxiv - Neuroscience 2019Quote: ... Secondary antibodies (Alexa Fluor-conjugated secondary antibodies, Molecular Probes) were then incubated at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Fluorescent secondary antibodies (Alexa-Fluor-conjugated antibodies; Molecular Probes) used were ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary antibodies used were Alexa-conjugated goat antibodies (Invitrogen) and were used at 1:1000.
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bioRxiv - Genetics 2020Quote: ... Secondary antibodies were Alexa Fluor antibodies (1:500, Invitrogen). Images were collected on a Zeiss Axio Imager M1 and were processed using ImageJ and Adobe Photoshop.
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bioRxiv - Cell Biology 2021Quote: ... or anti-Phospho-FOXO3a-antibody (1:200; Invitrogen Antibodies), respectively ...
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bioRxiv - Immunology 2022Quote: ... Western blot antibodies - GAPDH antibody (ThermoFisher cat# MA5-15738); pAKT 473 (Cell Signaling ...
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bioRxiv - Cell Biology 2024Quote: ... Secondary antibodies used were HRP-coupled secondary antibodies (Invitrogen) in a dilution of 1:10000 and Clarity Western ECL Substrate was used to visualize the signal ...
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bioRxiv - Molecular Biology 2023Quote: ... The primary antibody (6xHis tag monoclonal antibody; Invitrogen/ThermoFischer), secondary antibody (Polyclonal rabbit anti-mouse immunoglobulins/horseradish peroxidase ...
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bioRxiv - Plant Biology 2023Quote: ... antibodies and detected using fluorescently tagged secondary antibodies (Invitrogen, goat raised anti-mouse 488 and Anti-rabbit 555) ...
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bioRxiv - Developmental Biology 2023Quote: ... Secondary antibodies: Alexa Fluor conjugated secondary antibodies from Invitrogen (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... Other antibodies included antibodies to PINK1 (Invitrogen #PA5-86941) and β-actin (Sigma #A5441).
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bioRxiv - Developmental Biology 2024Quote: ... Secondary antibodies: Alexa Fluor conjugated secondary antibodies from Invitrogen (Thermofisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Alexa Fluor 568 anti-rabbit IgG (A11036) and Alexa Fluor 647 anti-rabbit IgG (A21245) from Thermo Fisher.
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bioRxiv - Cell Biology 2021Quote: ... Alexa Fluor 680 donkey anti-Rabbit and Alexa Fluor 790 Goat anti-Rabbit (Invitrogen by Thermo Fisher Scientific). Membranes were then imaged on a LI-COR Odyssey CLx.
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bioRxiv - Cell Biology 2021Quote: ... Alexa Fluor 680 donkey anti-Rabbit and Alexa Fluor 790 Goat anti-Rabbit (Invitrogen by Thermo Fisher Scientific). Membranes were then imaged on a LI-COR Odyssey CLx.
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bioRxiv - Developmental Biology 2021Quote: ... anti-rabbit COL5A1 (1:500; #PA5-83158) and anti-rabbit SIX3 (1:200; #PA5-103901) from Thermo Fisher; anti-mouse COL3A1 (1:300 ...
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bioRxiv - Microbiology 2023Quote: ... and rabbit anti-lysozyme (1:500, Novus) with goat anti-rabbit IgG Alexa Fluor 568 (Thermo Fisher Scientific); rabbit anti-villin (1:1000 ...
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bioRxiv - Biochemistry 2024Quote: ... Alexa 488-conjugated anti-rabbit IgG and Alexa 633-conjugated anti-rabbit IgG (all from Thermo Fisher Scientific). Mitochondria were stained with MitoTracker Orange CMXRos (Thermo Fisher Scientific ...
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Tuning trophoblast invasion in a gelatin hydrogel via soluble cues from the maternal-fetal interfacebioRxiv - Bioengineering 2020Quote: ... 5×5 minute washes in 0.1% Tween 20 solution in PBS were performed and samples were incubated in secondary antibody solution (Thermo Fisher A-21428 Goat Anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody AlexaFluor 555 ...
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bioRxiv - Cancer Biology 2019Quote: ... followed by the incubation with secondary antibodies (Alexa Fluor 568 donkey-anti-mouse #A10037, Alexa Fluor 488 donkey-anti-rabbit #21206, Life Technologies, 1:200 each, 2 hours, room temperature) and counterstaining with 4’6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were incubated over night with primary antibody rabbit-anti-Ca2+/calmodulin-dependent protein kinase II (1:200, #PA5-38239, Thermo Fisher, MA, USA; 1:500, #ab52476, Abcam, UK), rabbit-anti-parvalbumin (1:500 ...
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bioRxiv - Physiology 2019Quote: ... The blots were washed and probed with HRP-conjugated corresponding secondary antibodies (goat anti-rabbit, Cell Signaling Technology, #7074, 1:5000 or goat anti-mouse, Thermo Fisher Scientific, #62-6520, 1:5000). The blots were developed in ChemiDocTM-Touch Imaging system from Bio Rad.
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bioRxiv - Biophysics 2021Quote: ... The other end was ligated to a ∼190 bp biotin-labeled fragments created similarly by ApaI restriction of a 375 bp PCR amplicon of plasmid pBluKSP containing biotin-11-dUTP (Invitrogen, Life Technologies, Grand Island, NY, USA). All fragments were purified with PCR purification kits (Qiagen ...
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bioRxiv - Molecular Biology 2021Quote: cDNA was pooled down with 1.5 uL of Oligo C’ beads64 (in house magnetic beads prepared by immobilizing 2x Biotin oligonucleotides with Dynabeads™ MyOne™ Streptavidin C1; Thermo Fisher Scientific 65001), washed twice with 70% ethanol ...
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bioRxiv - Molecular Biology 2022Quote: ... Beads were carefully transferred to clean tubes before the last wash and resuspended in 100 microliters of elution buffer (12.5 mM biotin [Molecular Probes Life Technologies] in WB250 buffer) and incubated at room temperature with shaking for 60 minutes ...
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bioRxiv - Immunology 2024Quote: ... The native or chimeric 8H11 mAbs were biotinylated in a 20-fold molar excess of EZ-link NHS-PEG4-Biotin (Thermo Fisher Scientific, Waltham, MA, USA) following the manufacturer’s recommendations ...