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Citations for Thermo Fisher :
8401 - 8450 of 10000+ citations for Biotin Z Antibody Internalization Kit rabbit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... incubated with Alexa Fluor Tyramide 488 (1:100, Alexa Fluor 488 Tyramide SuperBoost Kit, goat anti-rabbit IgG, Invitrogen, Thermo Fisher Scientific, Ghent, Belgium) + hydrogen peroxidase 3% (1:100 ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-β-Amyloid (71-5800) and rabbit anti-β-Actin (PA1-183) were from Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... rabbit anti-Lef1 (1:100, Cell Signaling 2230S, and rabbit anti-Claudin 11 (1:100, Thermofisher, PIPA568608), and chicken anti-RFP (1:200 ...
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Pou3f1 orchestrates a gene regulatory network controlling contralateral retinogeniculate projectionsbioRxiv - Developmental Biology 2022Quote: ... 2ug of rabbit anti-POU3F1 #1894 and 2ug of rabbit isotype control IgG (Thermofisher Scientific, 02-6102) antibodies were added to the samples and incubated at 4°C overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... Aexa-488-conjugated goat anti-rabbit IgG (A11034) and Goat anti-Rabbit IgG-Alexa-594 (Invitrogen, A11072), Rhodamine Red-conjugated goat anti-mouse antibody (Jacksonimmuno ...
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bioRxiv - Neuroscience 2024Quote: ... anti-rabbit coupled to Alexa-fluor 594 (goat anti-rabbit, Thermo Fisher Scientific, #A-11012, 1:200), anti-guinea pig coupled to Alexa-fluor 647 (goat anti-guinea pig ...
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bioRxiv - Neuroscience 2023Quote: ... goat anti-rabbit Alexa Fluor 488 or goat anti-rabbit Alexa Fluor 568 (all from Thermo Fisher). Slides were washed twice in 1x PBS at room temperature and Hoechst 33342 (1:10,000 ...
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bioRxiv - Neuroscience 2024Quote: ... and 647 (1:500 for all; PIA32773-mouse; A21202-mouse; PIA32731TR-rabbit; PIA32794-rabbit; A21448-sheep, Invitrogen). The sections were examined using an AX-R Confocal Microscope coupled to a fully automated Ti2-E system (Nikon Instruments Inc ...
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bioRxiv - Neuroscience 2024Quote: ... OFC and mPFC sections were stained to visualize glial fibrillary acidic protein (GFAP) (primary antibody: mouse anti-GFAP antibody, Sigma-Aldrich; secondary antibody: donkey anti-mouse Alexa 594 antibody, ThermoFisher), and with fluorescent Nissl and DAPI ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... followed by Alexa Fluor® 488-conjugated goat anti-rabbit and Alexa Fluor® 568-conjugated goat anti-mouse antibodies (ThermoFisher Scientific A-11034 and A-11031), respectively ...
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bioRxiv - Microbiology 2020Quote: ... cells were washed multiple times and incubated with the secondary antibody Alexa Fluor 546 goat anti-rabbit IgG (H+L) (Invitrogen; 1:200 dilution in PBS-BSA-Saponin) in the presence of 1 μM of SiR-DNA for 2 hours in the dark ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were washed three times in PBS and incubated during 4 h in a blocking solution containing a secondary antibody to reveal TH (donkey anti-rabbit Alexa Fluor 594, Invitrogen A21207 lot 1890862 and 2145022 (1:400), RRID ...
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bioRxiv - Cancer Biology 2022Quote: ... The sections were incubated with Alexa Fluor 488 donkey anti-rabbit IgG and Alexa Fluor 633 goat anti-mouse IgG secondary antibodies for 1 h (1:200; Molecular Probes by Life Technologies, Waltham, MA, USA). Control slides were incubated with the secondary antibody alone ...
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bioRxiv - Neuroscience 2019Quote: ... Slices were washed three times with PBS and incubated for 1 hr at room temperature with a fluorescent secondary antibody (Alexa Fluor 546 goat anti-rabbit, Thermo Fisher Scientific catalog #A-11010, 1:500). Slices were washed three times with PBS and mounted onto microscope slides with Prolong Gold anti-fade medium (Invitrogen ...
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bioRxiv - Immunology 2019Quote: ... The cells were then washed 3 times in PBS/0.01% Triton-X and incubated with Alexa Fluor 594 anti-mouse and Alexa Fluor 488 anti-rabbit antibodies (Molecular Probes, 1:500 in PBS/0.01% Triton-X) for 45 minutes in the dark ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were then incubated in 5% normal donkey serum (NDS) for 1 hour before primary antibody incubation (mouse anti-GFP, 1:1500, Life Technologies; rabbit anti-TH, 1:2000, Millipore) overnight at room temperature with NDS and PBS-Tx ...
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bioRxiv - Cancer Biology 2022Quote: ... Embryos were incubated with appropriate secondary antibodies (goat α-chicken-AlexaFluor-488, goat α-rabbit-AlexaFluor-568, or goat α-mouse-AlexaFluor-647 (ThermoFisher Scientific, at 1:200) in 10% goat serum overnight at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... incubated for 2 hr at room temperature in the PBS buffer with goat anti-rabbit Alexa Fluor 488-conjugated secondary antibody (A-11008; Thermo Fisher Scientific, MA, USA, Invitrogen; 1:400), mounted on glass slides and covered with Vectashield ...
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bioRxiv - Microbiology 2022Quote: ... the cells were washed three times with the blocking buffer and incubated at room temperature with fluorophore-conjugated secondary antibodies (goat anti-mouse IgG Alexa 488, Thermo Fisher, Cat #: A11001, 1:500 dilution) (goat anti-rabbit IgG Alexa 555, Thermo Fisher, Cat #: A21428, 1:500 dilution) with counterstaining dyes (DAPI (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... the secondary antibodies anti-rabbit STAR RED (Abberior, # STRED-1002, dilution 1:50) and anti-mouse Alexa Fluor 594 (Thermo Fisher Scientific, # A-11005, dilution 1:100) were incubated at 4°C overnight in blocking solution ...
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bioRxiv - Molecular Biology 2022Quote: ... The slips were washed three times with PBS before the complementary anti-rabbit secondary antibody was added (Cat#A-11034, Life Technologies; 1:1000 dilution in blocking solution) and incubated for one hour at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed for three 10 min sessions in PBS then placed in secondary donkey anti-Rabbit Alexa Fluor 647-conjugated secondary antibody (A-21449; Thermo Fisher Scientific, MA, USA, Invitrogen; 1:200) in 2% donkey serum and 0.4% Triton X-100 in PBS for 2 hr at RT ...
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bioRxiv - Cell Biology 2022Quote: ... then incubated for 1 hour at room temperature with the following fluorophore-conjugated antibodies/dyes in 1% BSA-PBS: goat anti-rabbit Alexa-488 conjugate (Thermo Fisher Scientific A-11034, 1:1000 dilution), phalloidin Alexa-594 conjugate (Thermo Fisher Scientific A-12381 ...
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bioRxiv - Cancer Biology 2024Quote: ... antibodies were incubated overnight with rocking at 4°C and secondary antibodies (goat anti-rabbit [cat# 31462, Invitrogen, 1:10,000] or goat anti-mouse [cat#31432, Invitrogen, 1:10,000] IgG H&L cross absorbed HRP) were incubated for 2 hours at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... the preparations underwent a washing step and were then incubated in a mixture of secondary antibodies: anti-rabbit Alexa 488-conjugated IgG (Invitrogen, Waltham, USA; A-11008, goat, 1:700), anti-rabbit Alexa 555-conjugated IgG (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... followed by Alexa Fluor® 488-conjugated goat anti-rabbit and Alexa Fluor® 568-conjugated goat anti-mouse antibodies (ThermoFisher Scientific A-11034 and A-11031), respectively ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were Rabbit anti- SHANK1 (NovusBio Cat. #NB300-167, used at 1:150) and Mouse anti-PSD-95 (ThermoFisher Cat. #MA1-046, used at 1:200), incubated overnight (4°C) ...
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bioRxiv - Cell Biology 2023Quote: ... then incubated for 1 hour at room temperature with the following fluorophore-conjugated antibodies/dyes in 1% BSA-PBS: goat anti-rabbit Alexa-488 conjugate (Thermo Fisher Scientific A-11034, 1:1000 dilution), phalloidin Alexa-594 conjugate (Thermo Fisher Scientific A-12381 ...
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bioRxiv - Genomics 2023Quote: ... followed by overnight incubation at 4 °C in a primary antibody cocktail (Rabbit anti-NKX2.1, Abcam ab76013 at 1:200; Rat anti-SOX2, Thermo Fisher Scientific 14-9811-80 at 1:100). After washes with TBS-T ...
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bioRxiv - Cell Biology 2023Quote: The following antibodies were used for WB and immunofluorescence IF: rabbit Anti-VE-Cadherin (CatNo.361900, Life Technologies; 1:1000 for WB and 1:200 for IF), mouse Anti-ZO-1 (CatNo.339100 ...
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bioRxiv - Cell Biology 2023Quote: ... embryos were washed with PBST and then incubated with secondary antibodies (anti-rabbit-IgG AlexaFluor488-conjugated, Thermo Scientific, #A-21206, 1:500 dilution; anti-mouse-IgM AlexaFluor594-conjugated, Thermo Scientific, #A-21044, 1:500 dilution) in 1% NGS in PBST overnight at 4°C ...
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bioRxiv - Physiology 2024Quote: ... tissues/organs were washed with DPBS four times on a rocker over the course of an hour and subsequently incubated overnight at 4°C with a goat anti-rabbit Alexa Fluor® 568 IgG (H+L) secondary antibody (Molecular Probes, Life Technologies, Eugene, OR, USA) diluted 1:200 in 10% NSS made up in DPBS and protected from light ...
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bioRxiv - Neuroscience 2020Quote: All neuronal tracings were done by using combinations of tetramethylrhodamine-biotin dextran (Microruby, 3,000 MW, lysine fixable; Molecular Probes, Eugene, OR, USA, D-7162) and Alexa Fluor 488 dextran (10,000 MW ...
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bioRxiv - Biophysics 2021Quote: ... supplemented with 0.2 mM MgCl2 and 1 mM EGTA.66We prepared fluorescent tubulin (ATTO488- and ATTO565-labeled tubulin; AttoTech AD488-35 and AD565-35, AttoTech) and biotinylated tubulin (NHS-biotin, 21338, Thermo Scientific, Waltham, MA, USA) as previously described.67 In brief ...
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bioRxiv - Cancer Biology 2022Quote: ... The SKBR3 cell-membrane proteins were isolated by either the biotinylation of amino (EZ-Link Sulfo-NHS-SS-Biotin, Thermo Fisher Scientific, Rockford, IL) or oxidized glycan (EZ-Link Alkoxamine-PEG4-Biotin ...
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bioRxiv - Bioengineering 2020Quote: Purified S-ECD protein was biotinylated at a theoretical 1:3 molar ratio with EZ-Link NHS-PEG12-Biotin (Thermo Fisher Scientific, CAT#: 21313) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... The silicon device surfaces were functionalized with a previously described protocol using 3-mercaptopropyltrimethoxysilane (MPTMS) and N-gamma-maleimidobutyryloxysuccinimide ester (GMBS) NeutrAvidin-biotin chemistry (Thermo Fisher Scientific, Rockford, IL)[32 ...
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bioRxiv - Molecular Biology 2021Quote: 70 µg of total RNA were biotinylated by incubation with 160 µl of EZ-Link HPDP-Biotin (Thermo Scientific, 1 mg/ml in DMF), and biotinylation buffer (final concentration 10 mM Tris pH 7.4 ...
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bioRxiv - Cell Biology 2022Quote: ... formaldehyde-fixed paraffin-embedded sections were detected by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) assay (ThermoFisher Scientific; C10619; Waltham, MA) according to the manufacturer’s instructions.
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bioRxiv - Genomics 2022Quote: ... we performed end repair and then selectively purified biotin-tagged DNA using streptavidin coated beads (DYNAL™ MyOne™ Dynabeads™ Streptavidin C1, Invitrogen 65001). A-tailing and Illumina TruSeq adapter ligation (Illumina ...
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bioRxiv - Molecular Biology 2022Quote: ... Beads were carefully transferred to clean tubes before the last wash and resuspended in 100 microliters of elution buffer (12.5 mM biotin [Molecular Probes Life Technologies] in WB250 buffer) and incubated at room temperature with shaking for 60 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed once with PBS containing both calcium and magnesium and labeled with 0.5 mg of membrane-impermeable EZ-linkTM Sulfo-NHS-Biotin (Thermo Fisher Scientific, Rockford, IL, USA) per plate for 30 min at room temperature ...
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bioRxiv - Plant Biology 2023Quote: ... GE17–0851–01) for the removal of the excess biotin and incubated with equilibrated 100 μL Dynabeads™ MyOne™ Streptavidin (ThermoFisher Scientific, 65601) at 4 °C for 2 h with gentle rotation ...
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bioRxiv - Immunology 2024Quote: ... 2X106 Raji cells in log phase growth were incubated with 4 mg/ml biotin labeled goat anti-human IgM (Thermo Fisher Scientific, catalog #, #31778), 1 mg/ml mouse anti-human CD19 (SouthernBiotech ...
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bioRxiv - Developmental Biology 2021Quote: ... and anti FOXC2 (Goat, R&D AF6989) was conjugated to AlexaFluor-647 using Molecular Probes Antibody Labelling Kit (Invitrogen/ ThermoFisher). When labelling OTX2 ...
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bioRxiv - Immunology 2022Quote: ... we labeled the gp120-specific antibody (A32 mAb) with Allophycocyanin (APC) following the manufacturer instructions (Zenon Human IgG labeling kit, Invitrogen) and measured cell binding by flow cytometry ...
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bioRxiv - Immunology 2022Quote: ... IRAP and VAMP3 was performed using the BD intracellular staining kit and suitable species-specific fluorescent secondary antibodies (Life technologies).
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bioRxiv - Immunology 2019Quote: ... Decoy reagent to exclude non-specific binding to the GP tetramer was made by conjugating SA-APC to DyLight 755 using a DyLight 755 antibody labeling kit (ThermoFisher), washing and removing any unbound DyLight 755 ...
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bioRxiv - Biochemistry 2022Quote: ... Anti-CD18 antibody H52 (Developmental Studies Hybridoma Bank) was conjugated with Alexa Fluor 488 using Alexa Fluor 488 protein labeling kit (ThermoFisher) as described by manufacturer’s instruction ...
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bioRxiv - Neuroscience 2021Quote: ... sections were then incubated in poly-HRP-conjugated secondary antibody or HRP-conjugated streptavidin from Alexa Fluor 488 Tyramide SuperBoost Kit (Invitrogen) for 60 minutes and then incubated in Tyramide working solution according to manufacturer’s protocol ...