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Citations for Thermo Fisher :
801 - 850 of 10000+ citations for bta mir 486 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: DNA extraction was performed according to the bone and tooth protocol accompanying PrepFiler Express BTA Forensic DNA extraction kit (Applied Biosystems, Life Technologies Corporation, Carlsbad, CA, USA) [35] ...
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bioRxiv - Cancer Biology 2019Quote: Cells seeded in 24 well plates were co-transfected with miR-146a OE plasmid and either CD24 3′UTR or miR-146a promoter luciferase construct using LipofectamineTM 2000 (Invitrogen). The TOP-Flash and FOP-Flash reporters were also used under similar conditions ...
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bioRxiv - Cancer Biology 2021Quote: ... Internal controls were present in Columns 13 and 14 of each library plate (Pre-miR miRNA Precursor Molecules Negative Control #1 - Ambion AM17110; Pre-miR miRNA Precursor Molecules Negative Control #2 – Ambion AM17111 ...
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bioRxiv - Microbiology 2021Quote: ... On day 3 the cells were transfected with 60 pMol of either miR-122 or control microRNA (miR-122 p2-8) or anti-miR-122 LNA or control LNA (anti-miR-124) with 5uL of Lipofectamine 2000 (Invitrogen). Cells were harvested 2 days post-transfection and luciferase activity was measured.
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bioRxiv - Molecular Biology 2020Quote: ... or miR-324-3p.1 along with control miRNA or miR-324 duplexes using the Lipofectamine 3000 reagent (Thermo Scientific). After 2 days of transfection ...
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bioRxiv - Immunology 2020Quote: ... Probes for TaqManTM MicroRNA Assay (hsa-miR-424-3p, hsa-miR-424-5p, U6 snRNA control) were purchased from ThermoFisher. All the steps were performed according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... 20nM has-miR-129-5p miRIDIAN hairpin inhibitor (miR-129 inhibitor) or hairpin inhibitor negative control (inhibitor ctrl) (ThermoFisher Scientific) using LipofectamineTM RNAiMAX (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... Reverse transcription (RT) of total RNA was carried out using SuperScript™ IV One-Step RT-PCR System (Invitrogen), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: We performed a multiplex RT-qPCR assay using the TaqPath RT-PCR COVID-19 kit (Thermo Fisher CN A47814) together with the TaqPath 1-step master mix – No ROX (Thermo Fisher CN A28523) ...
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bioRxiv - Cancer Biology 2020Quote: ... Subsequent RT-qPCR analysis was performed using the SuperScript ® III First-Strand Synthesis System for RT-PCR (Thermofisher) and the following primers ...
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bioRxiv - Microbiology 2020Quote: ... The RT-qPCR reaction was prepared using the AgPath-ID-One-Step RT-PCR kit (Thermo Fisher Scientific, USA) adjusted to a volume of 12.5 μl [19] ...
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Turanose induced WOX5 restores symbiosis in the Medicago truncatula cytokinin perception mutant cre1bioRxiv - Plant Biology 2020Quote: ... 1μl of cDNA was used to set up real time qRT-PCR in a 20µl reaction system with 2X SYBR Green PCR master mix (Life Technologies). Experimental setup and execution were conducted using an ABI 7500 Fast Real-Time PCR system ...
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bioRxiv - Plant Biology 2021Quote: ... Quantitative real-time PCR (qRT-PCR) was performed using gene specific primers and SYBR Green (Life Technologies) on the LightCycler® 480 Instrument II (Roche) ...
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bioRxiv - Microbiology 2020Quote: ... were PCR-amplified with overlapping primers using Phusion Flash High-Fidelity PCR Master-Mix (Thermo Scientific, F548). PCR products were used as templates in a second PCR round using only the external primers that generated a linear dsDNA with the tetracycline resistance cassette flanked by the upstream and downstream sequences ...
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bioRxiv - Cancer Biology 2023Quote: ... qRT-PCR was performed using TaqMan universal PCR mix (#4324018) and predesigned TaqMan assay primers (Applied Biosystems). The following primers were used ...
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bioRxiv - Cell Biology 2024Quote: ... Quantitative PCR was carried out with specific primers using SYBR™ Green PCR Master Mix (Applied Biosystems) and StepOnePlus™ Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Cell Biology 2021Quote: ... and miR-34a-5p (ThermoFisher, Assay ID 000426). 1ul of cDNA was used for real-time PCR using TaqMan™ Universal PCR Master Mix (Applied Biosystems ...
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bioRxiv - Developmental Biology 2022Quote: ... for mature miR-9 or SuperScript III (Invitrogen) with random hexamers for pri-miRNAs ...
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bioRxiv - Molecular Biology 2019Quote: ... Anti-mir-125b was obtained from Applied Biosystems. mPAPD2 was PCR amplified from PASMC cDNA using the following primers ...
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bioRxiv - Neuroscience 2021Quote: ... the pcDNA6.2-GW/EmGFP-miR-neg plasmid (Invitrogen), containing a sequence not targeting any known vertebrate gene ...
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bioRxiv - Cell Biology 2020Quote: ... mirVana miR mimic negative control (Cat: 4464061, Ambion), hsa-miR-494-3p mimic (MIMAT002816 ...
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bioRxiv - Cell Biology 2022Quote: ... using the Human pre-miR miRNA library (Ambion), consisting of 837 miRNAs ...
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bioRxiv - Cell Biology 2023Quote: ... and Anti-miR™ miRNA Inhibitor (AM17000, ThermoFisher) were co-transfected by Lipofectamine RNAiMAX Transfection Reagent (13778100 ...
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bioRxiv - Neuroscience 2023Quote: ... and hsa-miR-182 (Thermo Fisher Scientific #002334). Relative expression of miRNAs analyzed in triplicates were normalized to the levels of Z30 and expressed as 2−ΔΔCt.
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bioRxiv - Cancer Biology 2023Quote: ... mir-96 mimic and antagomir (Ambion, nocodazole (CST) For Western immunoblots the following antibodies were used ...
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bioRxiv - Microbiology 2020Quote: ... The specific primer pairs used for qRT-PCR analyses were designed using the Primer Express version 2.0 software program (Applied Biosystems) (Table 2) ...
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bioRxiv - Genetics 2022Quote: ... of a PCR amplified region of the rgr-1 locus using OneTaq 2x Master Mix (forward primer DLO1140 5’-TGGAATGGGACTTCCTCTTG-3’ reverse primer DLO1141 5’-TTTCCAAAAGCCAGGACATC-3’) isolated using a GeneJET PCR Purification kit (ThermoFisher). The rgr-1(gk429013 ...
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bioRxiv - Microbiology 2019Quote: ... (CCTAYGGGRBGCASCAG forward primer; GGACTACNNGGGTATCTAAT reverse primer).53 PCR was done using AmpliTaq Gold 360 master mix (Life Technologies, Mulgrave, Australia) following a two-stage PCR protocol (29 cycles for the first stage ...
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bioRxiv - Plant Biology 2019Quote: ... The 31AΔAD fragment was amplified with the primer set (SmaI-31AF01; 31A-SmaIR01) and ligated into pJET1.2 cloning vector (Thermo Scientific), and the SmaI digested product was integrated into a SmaI site between the 35S promoter and Nos terminator sequences of the binary vector pGL1 (provided by Dr ...
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bioRxiv - Cell Biology 2022Quote: TaqMan qPCR was performed for Wnt2 using pre-designed TaqMan primer/probe sets (Life Technologies, Assay ID: Mm00470018 or Mm01156901) using Gadpdh (Assay ID ...
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bioRxiv - Microbiology 2022Quote: ... The nCoV_IP2 primer-probe set was used in combination with TaqMan fast virus 1-step master mix (Thermo Fisher Scientific) to detect viral RNA ...
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bioRxiv - Microbiology 2021Quote: ... The relative levels of the MafF mRNA was determined using TaqMan 746 Gene Expression Assay primer-probe sets (Applied Biosystems) Hs05026540_g1 ...
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bioRxiv - Microbiology 2021Quote: ... Data were processed as 2(−ΔΔCt) for quantification of cccDNA using chromosomal GAPDH DNA sequence (via primer-probe set Hs04420697_g1; Applied Biosystems) as an internal normalization control.
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bioRxiv - Molecular Biology 2019Quote: ... we amplified the sgRNA expression cassette from pENTR-PpU6-sgRNA-L1L2 using primers (Supplemental Primer Table) containing different Multisite Gateway attachment sites (attB) and subsequently recombined with the Multisite Gateway pDONR221 plasmid set (Invitrogen) using a BP clonase reaction following the manufacturer’s recommendations.
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bioRxiv - Physiology 2019Quote: ... Quantitative real-time PCR was conducted using primer sets in Supplementary Table 1 and mRNA levels were analyzed in duplicate using SYBR green (Invitrogen) using a thermocycler (BioRad CFX Connect Real Time System) ...
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bioRxiv - Microbiology 2021Quote: ... qPCR for Intracellular HBV DNA was performed by the 2(−ΔΔCT) method using chromosomal GAPDH DNA sequence (via primer-probe set Hs04420697_g1; Applied Biosystems) as an internal normalization control ...
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bioRxiv - Microbiology 2021Quote: ... and the relative levels of the KIF4 mRNA were determined using the TaqMan Gene Expression Assay with the primer-probe set Hs00602211_g1 (Applied Biosystems), while the ACTB expression (primer-probe set 748 Hs99999903_m1 ...
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bioRxiv - Genetics 2020Quote: ... 2-3ng of this DNA was used for each RT-qPCR reaction set up with primers specific to the centromeric dh repeat region using SYBR Master Mix (ThernoFisher) in QuantStudio3 (ThermoFisher). Fold enrichment was calculated using ΔΔCt method (27).
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bioRxiv - Genetics 2019Quote: ... and IP sample for each primer set was performed in triplicate in 10μl reactions using the PowerUP SYBR green master mix (Applied Biosystems) and 250nM of forward and reverse primers ...
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bioRxiv - Genomics 2021Quote: ... NGS was performed using primer sets that tiled the entire SARS-CoV-2 genome (Ion AmpliSeq SARS-CoV-2 Research Panel, ThermoFisher), with library preparation and sequencing performed on Ion Chef and S5 ...
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bioRxiv - Microbiology 2023Quote: ... Real-time qPCR (TaqMan) was used to analyzed cDNA levels with specific TaqMan primer and probe sets (Thermo Fisher Scientifc). Relative expression was determined using the ΔΔCT method using GAPDH as the standard control.
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bioRxiv - Microbiology 2022Quote: ... cDNA was generated by reverse transcription (RT) using specific reverse primers and SuperScript III Reverse Transcriptase (Invitrogen). Polymerase chain reaction (PCR ...
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bioRxiv - Neuroscience 2021Quote: ... The template cDNA was reverse transcribed with 5’-RACE RT primer and SuperScript III reverse transcriptase (Invitrogen), followed by poly(A ...
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bioRxiv - Biochemistry 2020Quote: ... The cDNAs were synthesized using Moloney murine leukemia virus RT with random primers (Invitrogen, Thermo Fisher Sci.). RNA levels of representative transcripts were analyzed in triplicate by quantitative PCR with SYBR™ Green using a Bio-Rad CFX thermal cycler ...
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bioRxiv - Biochemistry 2020Quote: ... The cDNAs were synthesized using Moloney murine leukemia virus RT with random primers (Invitrogen, Thermo Fisher Sci.). RNA levels of representative transcripts were analyzed in triplicate by quantitative PCR with SYBR™ Green using a Bio-Rad CFX thermal cycler ...
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bioRxiv - Immunology 2020Quote: ... cDNA synthesis was performed using a TCR specific primer (TTGGCACCCGAGAATTCCACTGHHHHHACAHHHHHACAHHHHHCACATTGATTTGGGA GTC) by using Maxima RT (Thermo scientific) according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... based protocol (71) followed by cDNA synthesis using random primers and Reverse Transcriptase (RT) enzyme (ThermoFisher Scientific). Using 100 ng of cDNA as the template and 0.5 µM of sequence specific primers ...
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bioRxiv - Microbiology 2020Quote: Quantitative PCR for CTCF was performed using Taqman probes/primers (Thermo Scientific). Specific primer probe sets used were Hs00902016_m1 for CTCF ...
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bioRxiv - Genetics 2019Quote: ... using amplicon-specific M13-capped PCR primers designed by VariantSEQr methodology (ThermoFisher) and deposited into the NCBI probe database (https://www.ncbi.nlm.nih.gov/probe/docs/projvariantseqr/) ...
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bioRxiv - Cancer Biology 2020Quote: ... Quantitative PCR using TaqMan primers (Supplementary file 1) (ThermoFisher Scientific, Waltham, MA) was employed to determine gene expression levels compared to control and normalized to either 18S or GAPDH.