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Citations for Thermo Fisher :
701 - 750 of 10000+ citations for bta mir 486 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Primers for real time PCR were obtained from Invitrogen (Vienna, Austria).
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bioRxiv - Molecular Biology 2020Quote: ... or hsa-miR-181-5p) (Bioneer) or with a Pre-miR miRNA Negative Control (Bioneer) using Lipofectamine 3000 (Invitrogen).
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bioRxiv - Neuroscience 2021Quote: ... The SPOP miR clone was designed using the BLOCK-IT miR RNAi design tool (SPOP accession number NM_001100496.1; ThermoFisher Scientific). Sense and antisense oligos for three distinct rat SPOP miRs beginning at SPOP cDNA basepair 212 ...
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bioRxiv - Pathology 2020Quote: ... Assay ID 245306_mat; 4427975, hsa-mir-23a-3p, Assay ID 000399; 4427975, cel-miR-39-3p, Assay ID 000200, Thermofisher). The relative expression of individual miRNA was calculated as ΔCt = mean Ct miRNA-mean Ct miR-39.
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bioRxiv - Neuroscience 2023Quote: ... the DNA fragment encoding miRNA for Dsp (5’-TGC TGT TGA GAT GGA CAT CAA TGC ACG TTT TGG CCA CTG ACT GAC GTG CAT TGG TCC ATC TCA A-3’ and 5’-CCT GTT GAG ATG GAC CAA TGC ACG TCA GTC AGT GGC CAA AAC GTG CAT TGA TGT CCA TCT CAA C-3’) was subcloned into pcDNA6.2-GW-EmGFP-miR using BLOCK-iT Pol II miR RNAi Expression Vector Kit (Invitrogen). The EmGFP and Dsp miRNA fragments were amplified by PCR from this vector and inserted between the KpnI and EcoRV sites of the pAAV-EF1α-DIO EYFP vector (#27056 ...
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bioRxiv - Cell Biology 2020Quote: ... Primer kits and Master Mix for RT-qPCR were purchased from Applied Biosystems (Foster City, CA). Monensin ...
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bioRxiv - Microbiology 2021Quote: ... Reverse transcription was performed with a universal IAV primer (31) using Maxima reverse transcriptase (RT; Thermofisher) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Reverse transcription (RT) from purified RNAs was performed using oligo(dT)20 primer (Superscript III, Invitrogen) or strand-specific RT primers as previously described (100) ...
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bioRxiv - Immunology 2020Quote: ... and complementary DNA (cDNA) was synthesized using a dT oligo primer and Superscript III RT (Invitrogen). After dilution in nuclease-free water ...
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bioRxiv - Immunology 2020Quote: ... 1μg RNA was reverse transcribed using oligo-dT primers and the Superscript III RT kit (Invitrogen). qPCR for each gene was performed in triplicate using SYBR Select (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... and β-actin was quantified by RT-qPCR using Taqman primer and probes (Thermo Fisher Scientific). Expression levels of BMP receptors were reported relative to GAPDH.
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bioRxiv - Genetics 2023Quote: ... and the random primer 5′-GTTTCCCAGTCACGATCNNNNNNNNNNNNNNN-3′ for the RT step and recombinant Taq polymerase (Invitrogen) for the PCR step using D388 specific primers 5′-GAGAGAAGGATTAGATTGTG-3′ and 5′-CCTGTATAATAGAAGTACCA-3′ ...
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bioRxiv - Microbiology 2024Quote: ... The extracted viral RNA was quantified by RT-qPCR using primers (Thermo Fisher Scientific, Waltham, MA) previously described32 and the SuperScript III OneStep RT-PCR System with Platinum Taq DNA Polymerase kit (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... qPCR reactions were set up using the primers indicated in Supplementary Table 1 and POWRUP SYBR Master Mix (ThermoFisher). qPCR was performed on the QuantStudio6 real-time PCR system (Applied Biosystems).118,119
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bioRxiv - Cancer Biology 2021Quote: ... LDHC expression was quantified by specific 5′FAM-3′MGB Taqman gene expression primer/probe sets (Hs00255650_m1, Applied Biosystems). MAP1B expression was quantified using primers for SYBR-based qPCR (F ...
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bioRxiv - Microbiology 2022Quote: ... GAPDH was used as endogenous gene control and was amplified using the commercial primer/probe set hs02786624_g1 (Applied biosystems). Primers for viral gene amplification were used at 500 nM each ...
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bioRxiv - Synthetic Biology 2024Quote: ... Relative gene quantification was performed using TaqMan primer probe set for LDLR (Hs01092524_m1) normalized against RPL19 (Hs02338565_gH) (Thermo Fisher). Each PCR reaction mix consisted of 0.5 μL primer probe ...
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bioRxiv - Developmental Biology 2021Quote: ... All primers for real-time PCR analysis used in this report were designed using Primer Express software 2.0.0 (Applied Biosystems).
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bioRxiv - Developmental Biology 2021Quote: ... and information for all primers used for ChIP assay and real-time PCR were designed using Primer Express software 2.0.0 (Applied Biosystems). The procedure for standard ChIP assay using H3K27me3 and Ezh2 (Cell Signaling ...
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bioRxiv - Molecular Biology 2022Quote: ... One-step RT-PCR using a SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase (Thermo Fisher Scientific, Waltham, MA, USA) was performed using a combination of primers [Mar-CPL-F and MarCP2R (876 bp)] designed specifically for minor CP amplification.
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bioRxiv - Microbiology 2023Quote: ... RNA from blood and tissue samples from NPs were used for RT-PCR amplification using SuperScript™ IV One-Step RT-PCR System kit (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) with a set of primers sequences targeting hantavirus nucleoprotein and polymerase genes [25,26] ...
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bioRxiv - Cell Biology 2020Quote: ... Quantitative RT-PCR (qRT-PCR) was performed using the SYBR Green system (Cat#F415L; Thermo Fisher). Primers are listed in KEY RESOURCES TABLE ...
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bioRxiv - Immunology 2019Quote: ... XBP1 transcripts (spliced and unspliced) were amplified by RT-PCR using PCR master mix (Thermo Fisher) and the following primers ...
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bioRxiv - Plant Biology 2020Quote: ... Real-time PCR was performed using the Power SYBR Green RT-PCR Reagents Kit (Applied Biosystems) in a 7900HT Fast Real-Time PCR machine (Applied Biosystems) ...
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bioRxiv - Neuroscience 2022Quote: ... Prdm13: qRT-PCR was performed using the Applied Biosystems StepOnePlus RT-PCR machine (Thermo Fisher Scientific) and a TaqMan Gene Expression Assay (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... Quantitative RT-PCR was performed using the Power SYBR Green PCR Master Mix Kit (Applied Biosystems) on a 7500 Fast Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Synthetic Biology 2020Quote: Total RNA samples for RT-PCR and q-PCR were extracted using the reagent RNAwiz (Ambion). After quantification of RNA ...
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bioRxiv - Cell Biology 2021Quote: ... Quantitative RT-PCR was performed using Power SYBR Green PCR Master Mix (4367659, Thermo Fisher Scientific) and the StepOnePlus system (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... Real-time RT-PCR was performed in a QuantStudio 5 Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Pathology 2019Quote: ... SYBR® Green PCR Master Mix and RT-PCR Reagents kit (Applied Biosystems, Foster city, USA) were used to do real time PCR on an ABI 7500 FAST REAL TIME PCR System (Applied Biosystems ...
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bioRxiv - Cell Biology 2019Quote: ... RT-PCR reactions were carried out in Step One Plus™ Real-Time PCR (Applied Biosystems) machine and amplification protocols were followed as indicated in the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... Real-time RT-PCR measurements were performed using the StepOnePlus Real-Time PCR System (Applied Biosystems). Samples were analyzed in triplicate in a total volume of 20 µl using the maxima SYBR green qPCR master mix according to manufacturer’s instructions (Thermo Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... Quantitative RT-PCR was performed on a 7300 or 7900 Real-Time PCR System (Applied Biosystems) using SYBR Green Master Mix (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2019Quote: ... Quantitative RT-PCR was performed with a SuperScript III Platinum One-Step qRT-PCR Kit (Thermofisher) in a CFX96 Touch Real-Time PCR Detection System (BioRad ...
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bioRxiv - Physiology 2023Quote: ... were determined by quantitative RT-PCR using 1X SYBR Green PCR Master Mix (Thermo Fisher Scientific). PCR reactions were performed on 10 ng of cDNA in the ABI PRISM 7900HT Sequence Detection System (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... All RT-PCR assays were done using the Viia 7 Real-time PCR System (Applied Biosystems). A standard curve was generated in order to obtain the exact number of copies in the tested sample ...
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bioRxiv - Developmental Biology 2023Quote: ... Quantitative RT-PCR was performed using an ABI Prism 7900HT PCR Sequence Detection System (Applied Biosystems) or ViiA7 (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2023Quote: ... All PCR reactions were monitored with real-time (RT-)PCR by spiking SYBR green (Thermo Fisher) into the reactions at 0.4x final concentration ...
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bioRxiv - Cancer Biology 2023Quote: ... Quantitative RT-PCR (qRT-PCR) assays were performed using Taqman probes (Applied Biosystems/Thermo Fisher Scientific): SERPINB3 (Hs00199468_m1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Quantitative RT-PCR (qRT-PCR) assays were performed using Taqman probes (Applied Biosystems/Thermo Fisher Scientific): SERPINB3 (Hs00199468_m1 ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative RT-PCR was performed with the SuperScript III Platinum One-Step qRT-PCR Kit (Invitrogen) using a Step One Plus Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2023Quote: ... RRT-PCR reactions were ran using AgPath-ID One Step RT-PCR Reagents (ThermoFisher Scientific #4387391) on a LightCycler 480 (Roche) ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative reverse transcription PCR (Q-RT-PCR) was performed using Taqman gene expression assays (Applied Biosystems) and reactions were run in duplicate on an AB7900HT Fast PCR System using manufacturers recommended amplification settings ...
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bioRxiv - Neuroscience 2024Quote: ... Relative mRNA expression was analyzed by quantitative RT-PCR using the QuantStudio PCR System (Applied Biosystems) and SYBR Green MasterMix (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: ... Pre-designed probe sets and TaqMan universal PCR Master Mix (2x, Thermo Fisher Scientific) were used as follows ...
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bioRxiv - Molecular Biology 2023Quote: ... The reaction was set up in a thermocycler (GeneAmp 9700 PCR System, Applied Biosystems). The PCR products were resolved on 1.8% agarose (USB ...
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bioRxiv - Genetics 2023Quote: ... qPCR reactions were set up using Power SYBR Green PCR Master Mix (Applied Biosystems) with 50 ng of total RNA ...
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bioRxiv - Genetics 2023Quote: ... qPCR reactions were set up using Power SYBR Green PCR Master Mix (Applied Biosystems) with 50 ng of total RNA ...
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bioRxiv - Genetics 2023Quote: ... qPCR reactions were set up using Power SYBR Green PCR Master Mix (Applied Biosystems) with a volume of the total RNA sample equivalent to 50 ng ...
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bioRxiv - Cancer Biology 2023Quote: ... Quantitative PCR reactions were set up using PowerUp SYBR Green Master Mix (Applied Biosystems) and performed on a Bio-Rad CFX96 machine ...