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Citations for Thermo Fisher :
801 - 850 of 1692 citations for Yeast Extract since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Cytosolic and chromatome extracts were quantified with Pierce BCA Protein Assay Kit (Thermo Scientific; #PIER23225).
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bioRxiv - Cell Biology 2023Quote: ... the recombinant adenovirus extract was injected into Slide-A-Lyzer Dialysis Cassettes (Thermo Fisher Scientific) and dialyzed in a dialysis buffer at 4 °C with stirring ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration in the soluble extract was quantified by using the BCA method (Thermo Scientific). For post-mortem material from FTD patients ...
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bioRxiv - Plant Biology 2024Quote: ... Protein quantification of the extracts was estimated using the BCA Protein Assay Pierce (Thermo Scientific) and bovine serum albumin as standard.
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bioRxiv - Microbiology 2023Quote: ... DNA extracts were quantified with the Qubit dsDNA HS Assay Kit (Life Technologies, Carlsbad, CA).
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bioRxiv - Cell Biology 2024Quote: ... to extract total RNA followed by DNase 1 treatment (18068-015, Thermo Fisher Scientific, USA) following manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Thirty μg of protein extracts was separated on 4–12% Bis-Tris gels (Thermo Fisher) in MOPS running buffer and transferred to nitrocellulose membranes using the Mini Trans-Blot Cell system (Biorad) ...
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bioRxiv - Neuroscience 2024Quote: ... Protein extracts were quantified using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific) and 40 μg of protein lysates were resolved on precast 3-8% gels in Tris-acetate buffer and then transferred to nitrocellulose membranes (all from Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... and whole cell extract was quantified using the BCA Protein Assay Kit (Life Technologies, 23250) following manufacturer instructions ...
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bioRxiv - Microbiology 2024Quote: ... and concentrations of DNA extracts were quantified using a Qubit fluorometer (Invitrogen, Carlsbad, California, USA). DNA extracts were stored frozen at -20 °C until analysis.
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bioRxiv - Microbiology 2023Quote: ... Nuclear extracts were prepared using the NE-PER Nuclear and Cytoplasmic Extraction Reagent kit (ThermoFisher) on ice at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... melongena PI 555598 acylsugar extracts were dried down in vacuo with centrifugation (Savant, ThermoFisher Scientific). Dried acylsugar extracts were dissolved in 1 mL of methanol ...
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bioRxiv - Plant Biology 2023Quote: Protein extracts were quantified using the Pierce Detergent Compatible Bradford Assay Kit (ThermoFisher, cat#23246) on a Promega Glomax-Multi+ plate reader.
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bioRxiv - Cell Biology 2020Quote: ... The chemiluminescent signal in yeast samples was generated by the SuperSignal West Pico Plus chemiluminescent substrate (Thermo Scientific) and detected on a G-box transilluminator (Syngene).
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bioRxiv - Cell Biology 2020Quote: ... 7- Amino-4-Chlormethylcumarin (CMAC) was part of the Yeast Vacuole Marker Sampler Kit (Y7531) from Thermo Fisher and staining was performed according to manufacturer recommendations ...
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bioRxiv - Genetics 2019Quote: We extracted the DNA of these 20 hybrid samples using the Yeast DNA Extraction Kit (Thermo Scientific 78870). After extraction ...
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bioRxiv - Cell Biology 2019Quote: ... SD media used for growth of yeast cultures contained: 2% w/v dextrose (Thermo Fisher Scientific, Waltham, MA), 13.4 g/L Yeast Nitrogen Base without Amino Acids (BD Biosciences ...
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bioRxiv - Systems Biology 2019Quote: ... For fraction separation a pellet was resuspended in 75 ml Y-Per yeast protein extraction reagent (Thermo Scientific) supplemented with 0.1mM PMSF and incubated at room temperature with agitation for 20 min ...
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bioRxiv - Bioengineering 2020Quote: ... All sequences were cloned into the entry vector pDRf1-GW for yeast expression (28) through Gateway cloning (Invitrogen).
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bioRxiv - Plant Biology 2021Quote: ... and cloned into the yeast expression vector pFL61-Gateway (Desbrosses-Fonrouge et al., 2005) using LR clonase (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... The hybridization buffer was composed of encoding wash buffer supplemented with 0.1% (wt/vol) yeast tRNA (Life Technologies), 1% (vol/vol ...
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bioRxiv - Genomics 2020Quote: ... SD media used for growth of yeast cultures contained: 2% w/v dextrose (Thermo Fisher Scientific, Waltham, MA), 13.4 g/L Yeast Nitrogen Base without Amino Acids (BD Biosciences ...
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bioRxiv - Molecular Biology 2022Quote: All plasmids used in yeast two-hybrid assays were cloned with the Gateway® Gene Cloning Technology (Invitrogen) and transformed in the AH109 and Y187 haploid yeast strains (Clontech) ...
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bioRxiv - Biochemistry 2021Quote: ... The plasmid pPICZ used to obtain the yeast α-factor secretion signal sequence and was obtained from ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... each slide was covered in 300 μl of a hybridization buffer containing yeast tRNA (Invitrogen; Carlsbad, CA, USA), salmon sperm DNA (Ambion) ...
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bioRxiv - Cancer Biology 2021Quote: The yeast display selection process was started with two rounds of selections using streptavidin-coated dynabeads (Thermo Fisher) loaded with PXDN-con4-biotin ...
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bioRxiv - Immunology 2021Quote: ... Tp2 and Tp9 were expressed for either surface display in the pYD1 vector (Yeast Display Vector kit, Invitrogen) or for intracellular expression using the pYES2/NTC vector (N-terminal Xpress and C-terminal V5 epitope ...
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bioRxiv - Microbiology 2022Quote: Yeast strains were routinely grown in synthetic drop-out media (SD) with 2% (w/v) Dextrose (Fisher Scientific), 0.67% (w/v ...
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bioRxiv - Microbiology 2022Quote: ... Yeast cells were washed with PBS and total RNA was isolated using Trizol (Life Technologies, Carlsbad, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... were blocked for 2 hr at 4 °C in lysis buffer containing 1 mg/ml yeast tRNA (Ambion) and washed twice with 1 ml lysis buffer ...
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bioRxiv - Synthetic Biology 2023Quote: ... Genomic DNAs of the positive transformants were extracted using Pierce Yeast DNA Extraction Reagent Kit (Thermo Scientific™). The target regions were confirmed by Sanger sequencing performed at GENEWIZ ...
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bioRxiv - Molecular Biology 2023Quote: ... ceSMSγ and ceSMSr cDNAs were cloned into yeast expression vector pYES2.1/V5-His-TOPO and mammalian expression vector pcDNA3.1/V5-His-TOPO (Invitrogen) as described previously(18).For expression studies in Drosophila S2 cells ...
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bioRxiv - Microbiology 2023Quote: ... RNA was extracted and concentrated by TRizol and ethanol precipitated with Glycogen and yeast tRNA (Thermo Fisher Scientific). Products were subjected to denaturing PAGE and visualized in X-ray films after incubation at -80 ℃ for 48 h.
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bioRxiv - Plant Biology 2023Quote: Yeast two-hybrid screening was performed according to the manufacturer’s instructions using the ProQuestTM Two-hybrid system (Invitrogen). AHL10 full length coding sequence was cloned into the entry vector pENTR-D-TOPO and recombined into the bait destination vector pDEST32 with GAL4 DNA binding domain (DBD) ...
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bioRxiv - Cell Biology 2023Quote: ... Beads were then blocked using 1ml lysis buffer with 5µl RNAseOUT and 50µg yeast tRNA (Thermo Fisher Scientific) for 1 hour at 4°C with slow rotation ...
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bioRxiv - Plant Biology 2024Quote: The yeast two-hybrid assay was performed according to previous publications using the ProQuest Two-Hybrid System (Invitrogen) following the manufacturer’s instructions52 ...
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bioRxiv - Biochemistry 2024Quote: For colony PCR and yeast genotyping we used Phire Green Hot Start II PCR Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2024Quote: ... This growth medium contained Yeast Nitrogen Base without amino acids and ammonium sulfate (YNB; DF0335159, Thermo Fisher Scientific), 10.0 g/L 13C-glucose (CLM-1396-PK ...
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bioRxiv - Genetics 2021Quote: Twenty microliters of DO lipid extract were injected by an Ultimate 3000 RSLC autosampler (Thermo Scientific) coupled to a Q Exactive Focus mass spectrometer by a HESI II heated ESI source ...
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bioRxiv - Cell Biology 2020Quote: ... Metabolite extracts were analyzed using a ThermoFisher Scientific Q Exactive Basic Orbitrap Mass Analyzer (ThermoFisher Scientific) in negative mode ...
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bioRxiv - Cell Biology 2020Quote: ChIP experiments were performed on chromatin extracts according to the manufacturer’s protocol (MAGnify ChIP; Life Technologies) by O.N ...
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bioRxiv - Microbiology 2019Quote: ... Residual DNA in RNA extracts was digested using the TURBO DNA-free kit (Thermo Fisher Scientific). DNA removal was checked by gel electrophoresis after PCR (16S rRNA gene ...
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bioRxiv - Developmental Biology 2021Quote: The protein extracts were mixed with 4X Bolt™ LDS Sample Buffer (Invitrogen, cat. no. B0008) and 250 mM DTT and boiled for 5 min ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Total protein extracts were prepared by using B-PER® Bacterial Protein Extraction Reagent (Thermo Scientific) as per manual ...
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bioRxiv - Molecular Biology 2020Quote: ... and the genomic DNA was extracted from cells using Quick Extract DNA Extraction Solution (ThermoFisher, NC9904870) according to the instructions ...
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bioRxiv - Genomics 2020Quote: ... Protein concentrations of all extracts were measured using Pierce BCA Protein Assay Kit (Thermo Fisher, #23225). Samples for immunoprecipitation were prepared at a final concentration of 15 μg/μL protein in IP buffer up to 500 μL ...
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bioRxiv - Molecular Biology 2019Quote: ... The quantity and quality of the RNA extract was determined with the Nanodrop Spectrophotometer (Thermo Scientific) and the Agilent 2200 TapeStation ...
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bioRxiv - Genetics 2020Quote: ... Pulldowns were performed with 1.2 mg of nuclear extracts using M-280 paramagnetic streptavidin beads (Invitrogen) as previously described (Chatzinikolaou et al. ...
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bioRxiv - Biochemistry 2019Quote: ... The quantity and quality of RNA extracts were assessed using a Nanodrop 2000 (Thermo Scientific, USA). Samples were diluted up to 100 to 200 ng/μl concentration using RNase-free water ...
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bioRxiv - Microbiology 2019Quote: ... DNA extracts were quantified using the Qubit High Sensitivity DS-DNA assay (Invitrogen/Thermo Fisher, Inc) and normalized to 0.2 ng/μL ...