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Citations for Thermo Fisher :
701 - 750 of 1692 citations for Yeast Extract since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: High molecular weight DNA was extracted from yeast culture/ mixed culture using Qubit BR DNA kit (Invitrogen) genomic DNA kit following the manufacturer’s instructions at geneOmbio technologies Pvt ltd ...
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bioRxiv - Synthetic Biology 2020Quote: ... Genomic DNA was extracted from pellets using the Yeast DNA Extraction Kit from ThermoFisher (catalog number 78870) according to the instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... 150 ng of total RNA was incubated with 75 U of yeast poly(A) polymerase (PAP) (Affymetrix) in the presence of GTP/ITP mix at 37°C for 60 min ...
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bioRxiv - Immunology 2021Quote: ... Yeast codon-optimised sequences of the Tp1 and Tp2 gene were synthesized by GeneArt (Thermo Fisher Scientific) and codon-optimised sequences for expression of Tp2 and Tp9 gene in eukaryotic cells were kindly provided by Dr ...
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bioRxiv - Microbiology 2021Quote: ... Depletion of rRNA was accomplished using the Ribominus Yeast and Bacteria transcriptome isolation kit (Invitrogen, Waltham, MA) and included ethanol precipitation with glycogen to concentrate remaining mRNA ...
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bioRxiv - Plant Biology 2022Quote: ... and subsequently cloned into pGILDA and pB42AD yeast-two-hybrid vectors (Clonetech) via LR Gateway Reactions (ThermoFisher). To introduce T653L and Y643* mutations into ARFd1 entry clone ...
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bioRxiv - Molecular Biology 2023Quote: ... Yeast cells were then harvested for RNA extraction using a RiboPure RNA Purification Kit (Invitrogen, cat#AM1926), which involves mechanical cell wall disruption ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µL 32P-phosphorylated target RNA (∼1000 cpm/µL) and 1 µg yeast tRNA (Thermo Fisher Scientific) was added to each reaction ...
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bioRxiv - Biophysics 2023Quote: ... live yeast cells were immobilized in 8-well microscope chamber slides (Nunc Lab-tek, Thermo Fisher Scientific) pre-coated with 1-2 mg/mL concanavalin-A (MP Biomedicals) ...
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bioRxiv - Biophysics 2023Quote: ... live yeast cells were immobilized in 8-well microscope chamber slides (Nunc Lab-tek, Thermo Fisher Scientific) pre-coated with 1-2 mg/mL concanavalin-A (MP Biomedicals) ...
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bioRxiv - Plant Biology 2023Quote: ... Yeast colony polymerase chain reaction (PCR) was done using the Phire polymerase following manufacturer’s instructions (Thermo Fisher).
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bioRxiv - Developmental Biology 2022Quote: ... hybridization buffer had additional 50 µg/ml yeast tRNA and 1x Denhardt’s solution (Invitrogen, Waltham, Massachusetts. U.S.), all post-hybridization washes were carried out using 5x SSC ...
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bioRxiv - Molecular Biology 2023Quote: ... Stock probes were diluted 1:1000 in RNA FISH hybridization buffer preheated to 42°C (15% deionized formamide, 10% dextran sulfate, 2× SSC from Ambion, 0.02% BSA, 0.2mg/mL Baker’s yeast tRNA from Ambion). Plate was placed in plastic bag with wet paper towels and incubated at 42°C for 1 hour ...
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bioRxiv - Synthetic Biology 2023Quote: ... and a rabbit polyclonal anti-eEF2 (yeast) antibody at a 1:25,000 dilution (sample loading control) (ThermoFisher), followed by a secondary anti-rabbit antibody at a 1:20,000 dilution (Sigma Aldrich).
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bioRxiv - Molecular Biology 2024Quote: ... 150 ng of total RNA was incubated with 75 U of yeast poly(A) polymerase (PAP) (Affymetrix) in the presence of GTP/ITP mixure at 37°C for 60 min ...
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bioRxiv - Biophysics 2024Quote: ... we inoculated 1 liter of BMGY medium (this and the other yeast media were obtained from Invitrogen) with 10 ml of an overnight culture grown in YPD medium with Zeocin (100 ug/ml) ...
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bioRxiv - Microbiology 2020Quote: ... DNA extracts were quantified and quality checked using a Qubit fluorometer (Invitrogen, Carlsbad, CA, USA) with Qubit dsDNA HS assay (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... RNA extracts were quantified using the QuBit RNA HS Assay Kit (Thermo Fisher Scientific, Waltham). DNAse treatment ...
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bioRxiv - Evolutionary Biology 2019Quote: ... After DNase treatments of RNA extracts using the Turbo DNA-free kit (Ambion, Life technologies), cDNAs were produced by poly(A)-tailing (Poly(A ...
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bioRxiv - Evolutionary Biology 2019Quote: ... After DNase treatments of RNA extracts using the Turbo DNA-free kit (Ambion, Life technologies), cDNAs were produced by poly(A)-tailing (Poly(A ...
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bioRxiv - Neuroscience 2021Quote: ... Cytoplasmic and nuclear protein extracts were collected using the NE-PER kit (Thermo Fisher Scientific). Protein concentrations were measured using the Pierce BCA Protein Assay kit (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: All DNA extracts were suspended in 100 μl of DNA/DNase-free water (ThermoFisher Scientific). Extracted DNA was thereafter stored at −20 °C until further use ...
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bioRxiv - Molecular Biology 2021Quote: ... The concentration of the oocyte extract was measured using the Bradford assay (Thermo Fisher, 23236) and was usually at 35–45 mg/ml.
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bioRxiv - Cancer Biology 2019Quote: ... supplemented with Bovine Pituitary Extract (BPE, 0.05 mg/ml, Thermo Fisher Scientific, catalog #17005-042), human recombinant Epidermal Growth Factor 1-53 (EGF 1-53 ...
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bioRxiv - Cancer Biology 2019Quote: ... The cultured cells were treated to extract proteins using a protein extraction kit (Thermo Fisher), with bovine serum albumin as the standard ...
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bioRxiv - Molecular Biology 2019Quote: Protein extracts were separated in 10% RunBlue SDS gels and transferred to PVDF membranes (Invitrogen). After blocking ...
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bioRxiv - Cell Biology 2019Quote: ... Extracts were analyzed by SDS-PAGE using a 10% Bis–Tris Nu-PAGE gels (Invitrogen) and transferred onto PVDF membranes (Millipore) ...
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bioRxiv - Synthetic Biology 2019Quote: ... Cell extracts were prepared with B-PER Complete Bacterial Protein Extraction Reagent (Thermo Fisher Scientific) and the amount of protein was measured with the Bradford Protein Assay (Bio-Rad ...
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bioRxiv - Cancer Biology 2020Quote: ... and 50 μL metabolite extracts were transferred to conical storage plates (ThermoFisher, CAT AB-1058), sealed (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: Whole cell lysate extracts were prepared M-PER Mammalian Protein Extraction Reagent (Thermo Scientific, 78503) supplemented with 1X Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Protein extracts were separated on a NuPAGE 4–12% Bis-Tris pre-cast gel (Invitrogen) for 1 h at 200 V and transferred onto a methanol-activated PVDF membrane at 400 mA for 1 h ...
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bioRxiv - Neuroscience 2020Quote: ... Extracts were separated by electrophoresis on NuPAGE 4-12% Bis-Tris gels (Thermo Fisher Scientific) in MOPS buffer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Total cell extracts were incubated for 3 h with 20 μl protein A Dynabeads (Invitrogen) and 2 μg of RASSF1A antibody (Atlas ...
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bioRxiv - Biochemistry 2021Quote: ... Cholesterol content of nuclear extracts was measured using an Amplex Red cholesterol assay kit (Invitrogen).
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bioRxiv - Cell Biology 2021Quote: ... Protein extracts were then incubated for 1 hour with protein A-conjugated Dynabeads (Life Technologies), after which the beads were washed with lysis buffer at incremental KOAc concentrations (100 mM ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein concentration of the whole cell extract was determined using the NanoDrop (Thermo Scientific). Equal amounts of whole cell extract were added to SDS PAGE gel wells ...
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bioRxiv - Cell Biology 2021Quote: ... Cell protein extracts were resolved by Bis-Tris polyacrylamide gel electrophoresis gels (Thermo Fisher Scientific) and run in either MOPS or MES buffer (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... 10.5 µL of the extract was reverse transcribed using Superscript VILO (ThermoFisher Scientific, Reinach, Switzerland) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Extract was precleared by incubating overnight with 5 ug of mouse polyclonal IgG (Invitrogen, #31903) in the presence of 100 ug RNase A (Fisher scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with recombinant EGF and bovine pituitary extract according to the manufacturer’s instructions (Thermo Fisher), as well as 100 μg/ml Primocin (Invivogen) ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein extracts were then incubated with 25 μl of protein G dynabeads (Thermo Fisher, 10004D) for 1 hour at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: All DNA extracts were quantified using Qubit dsDNA high sensitivity Assay Kits (Thermo Fisher Scientific). All initial libraries (referred to as “pre-capture libraries” ...
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bioRxiv - Molecular Biology 2022Quote: ... Ten mosquitoes as a pool were used to extract total RNA using TRIzol reagent (Invitrogen). Three independent biological replicates were performed ...
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bioRxiv - Microbiology 2022Quote: ... Protein extracts were separated on 4-12% Bis-Tris mini protein gels (NuPage®, Invitrogen) and blotted on polyvinylidene fluoride membranes (Bio-Rad) ...
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bioRxiv - Immunology 2022Quote: ... Equal concentrations of protein extract were loaded into 4%–12% NuPAGE BisTris gels (Life Technologies) and transferred to PVDF membranes utilizing the Transblot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Cell Biology 2022Quote: ... 10 μL of extract was injected by a Vanquish Split Sampler HT autosampler (Thermo Scientific).
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bioRxiv - Cell Biology 2021Quote: ... equal volume of the total RNA extract was further purified with DNA-free Kit (Invitrogen).
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African Swine Fever Virus CD2v protein promotes β-Interferon expression and apoptosis in swine cellsbioRxiv - Microbiology 2020Quote: ... Whole cell extracts were prepared at 8 h pt using RIPA lysis buffer (Thermo Scientific). Reciprocal co-immunoprecipitations were performed using active motif Co-IP kit (Active Motif ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein extracts were quantified using the Pierce BCA Protein assay kit (Thermo Fisher Scientific). Protein samples were boiled at 95 °C in Laemmli buffer for 5 min ...
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bioRxiv - Cell Biology 2021Quote: ... 10 µg of protein extract determined by bicinchoninic acid assay (BCA kit, Thermofisher Cat# PI23227), were separated by 4-12% SDS-PAGE and transferred to nitrocellulose membranes ...