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Citations for Thermo Fisher :
801 - 850 of 10000+ citations for Piggybac Transposable Element Derived 3 PGBD3 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... suspended in arrest-mixture assay buffer (no ATP) with 3% BSA and 1:400 anti-CARMIL1 antibody (ThermoFisher, 27133-1-AP). After incubation for 2 hr at RT ...
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bioRxiv - Bioengineering 2024Quote: ... sections were similarly processed and incubated with mouse anti-Aggrecan (BC-3) primary antibody (1:50 in 2.5% NGS; Cat. No. MA3-16888; Thermo Fisher Scientific) and rabbit anti-Col-II primary antibody (1:100 in 2.5% NGS ...
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bioRxiv - Bioengineering 2024Quote: ... the slices were rinsed 3 times in PBS and incubated in the corresponding secondary antibody (1:500, Invitrogen, thermo Fisher Scientific) at 4°C overnight on a rotator ...
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bioRxiv - Bioengineering 2024Quote: ... the slices were rinsed 3 times in PBS and incubated in the corresponding secondary antibody (1:500, Invitrogen, thermo Fisher Scientific) at 4°C overnight on a rotator ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were washed 3 times with 0.1% Triton X-100 in PBS and incubated with secondary antibodies (Molecular Probes or Thermo Fisher) and DAPI (Sigma-Aldrich® ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed 3 times with 0.05% Triton X-100 in PBS and incubated with secondary antibodies (Molecular Probes or Thermo Fisher) and DAPI (Sigma-Aldrich® ...
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bioRxiv - Neuroscience 2024Quote: ... Retinas were washed in 1x PBS for 3×30 minutes at RT and transferred to secondary antibody solution containing 1:1000 goat anti-mouse Alexa 488 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... in 0.05 M TBS and then incubated in the dark for 2 h at room temperature with secondary antibodies: donkey anti-mouse Alexa Fluor 488 (3 µl/ml; Life Technologies) and donkey anti-rabbit Alexa Fluor 594 (5 µl/ml ...
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bioRxiv - Neuroscience 2024Quote: ... slices were washed 3 times in PBS for 5 minutes before secondary antibody solution (species-appropriate, Life Technologies Alexa Fluor-conjugated) 1:1000 in PBS/Triton/azide for 3 hours at room temperature ...
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bioRxiv - Systems Biology 2024Quote: ... The cells were blocked with 3% BSA/10% normal goat serum followed by incubation with primary antibody (1:100) against ROCK1 (#PA5-22262; Thermo Scientific). F-ACTIN staining for observing cytoskeletal changes in SHROOM3-transfected cells was done using 100nM Acti-stain 488 phalloidin (#PHDG1 ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were blocked with Interceptor reagent (Li-COR 927-70001) and antibodies were diluted with 3% BSA (Fisher Scientific, BP9703-100). The antibodies are listed in Supplementary data table 2 ...
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bioRxiv - Bioengineering 2024Quote: Samples were washed 3 times with DPBS before being incubated with secondary antibodies and 4′,6-diamidino-2-phenylindole (DAPI, Invitrogen, 10125092) at 1:500 in the blocking solution ...
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bioRxiv - Cell Biology 2024Quote: The following primary antibodies were used for immunofluorescence microscopy and immunoblotting: rabbit anti-claudin-3 pAb (34-1700; Thermo Fisher Scientific); mouse anti-claudin-4 mAb (32-9400 ...
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bioRxiv - Cell Biology 2024Quote: ... membrane was washed 3 x 5 min in PBS-T and incubated for 1 hour at RT with secondary antibody anti-mouse (Invitrogen #31439) or anti-rabbit (Invitrogen #31460 ...
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bioRxiv - Developmental Biology 2024Quote: ... The secondary antibody solution was washed off the slides 3 times and the slides were mounted using Fluoromount-G™ with DAPI (Invitrogen). Images were taken under a Leica DMI6000 B microscope and analyzed with FIJI ...
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bioRxiv - Neuroscience 2022Quote: ... 10% 3 kD or 10 kD biotinylated dextran amines (3 kD BDA, Invitrogen D7135 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... #2: 5’-gatcccGATTACTCGGCCATGATCAAAgcttcctgtcacTTTGATCATGGCCGAGTAATCttt ttta-3’ and 5’-agcttaaaaaaGATTACTCGGCCATGATCAAAgtgacaggaagcTTTGATCATGGCCGAGTAATgg-3’ were purchased from Invitrogen. The DNA oligo pairs were annealed and inserted into pEntryCla12-chickU6 shuttle vector using BamHI/HindIII site.
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bioRxiv - Cell Biology 2020Quote: ... Lipin-1 siRNA 5’-GAAUGGAAUGCCAGCUGAA-3’ and 3’-UUCAGCUGGCAUUCCAUUC-5’ (Invitrogen; HSS118307 (Sigma); optineurin siRNA (Invitrogen 4392420) ...
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bioRxiv - Bioengineering 2023Quote: ... 1-ethyl-3-(3-dimethyl-aminopropyl) carbodiimide (EDC; 22980) was purchased from Thermofisher Scientific (MA) ...
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bioRxiv - Molecular Biology 2021Quote: E14Tg2a mouse embryonic stem cells (mESCs) (129/Ola isogenic background) and derived clones were cultured in serum-free DMEM/F12 (Gibco) and Neurobasal (Gibco ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA levels in LSK+ cells and BM-derived macrophages (BMDMs) were determined by real-time qPCR using SYBR Green master mix (ThermoFisher) (10 min at 95°C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... we first extracted total RNA from pools of tissue derived from ten like individuals using TRIzol Reagent (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: DIV80 human iPSC-derived astrocytes were incubated with the calcium indicator Fluo-4 AM (1μM; ThermoFisher Scientific; Cat. No. F14201) for 30 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... and Cas9-mediated lesions were detected by Sanger sequencing of a PCR amplicon derived from genomic DNA (PureLink Genomic DNA kit, Invitrogen).
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bioRxiv - Biochemistry 2020Quote: ... Previously derived raw LC-MS data for HAP1 cell cultures supernatant [16] was re-analysed with TraceFinder (Thermo Fisher Scientific) software to extract and quantify the lactate peaks ...
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bioRxiv - Immunology 2020Quote: ... The peptide samples derived from individual patients were then individually labelled using the TMT10plex mass tag kit (Thermo scientific, USA) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Surface-stained ESC-derived cells were fixed/permeabilized and stained using the eBioscience FOXP3/Transcription Factor Staining Buffer Set (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: (Experiment A) Pancreatic derived 1.1B4 cell was seeded as 1.5×105 cells / ml to RPMI-1640 medium (GIBCO, Tokyo, Japan) in 6 well plate ...
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bioRxiv - Developmental Biology 2021Quote: Total nuclear RNA extraction and DNAse treatment of TgBAC(tcf21:BirA-Cherry;βactin:Avi-Cerulean-RanGap)ox144 adult-derived epicardial nuclei were carried out using the RNAqueous Micro Kit (Life Technologies) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... We measured packaging of the RNA from an HIV-1-derived GFP vector constructed from pLenti6/V5-DEST (ThermoFisher, Inc.). A Psi-version of this vector was constructed ...
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bioRxiv - Bioengineering 2021Quote: ... 105 PBMCs derived from a healthy human donor were stimulated with T cell activating beads (anti-CD3/CD28 Dynabeads, ThermoFisher) at a 1:1 PBMC:bead ratio in each well containing MSCs ...
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bioRxiv - Microbiology 2021Quote: ... whilst the growth medium of T-REx-293- derived cell lines stably transfected with pcDNA4/TO-based plasmids was further supplemented with zeocin (100 µg/mL, Gibco).
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bioRxiv - Cancer Biology 2020Quote: All melanoma cell lines and patient-derived primary melanoma cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% (v/v ...
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bioRxiv - Plant Biology 2021Quote: ... driving eGFP expression derived from pUBN-Dest26 was assembled with MCP contained in pDONR2018 using the Gateway cloning technique (Invitrogen) creating plasmid pUb::eGFP/MCP-NLS ...
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bioRxiv - Biochemistry 2021Quote: B16-F1 cells and derived CapZa1/2 CRISPR#1 and CapZb KO#10 were cultured in DMEM (4.5 g/L glucose; Invitrogen, Germany), supplemented with 10% FCS (Gibco) ...
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bioRxiv - Synthetic Biology 2021Quote: All yeast strains were derived from parental strains Fy251 [American Type Culture Collection (ATCC) 96098] or the two-hybrid strain MaV20330 (Invitrogen). Yeast transformations were carried out using the lithium acetate method48 ...
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bioRxiv - Biophysics 2020Quote: Human umbilical cord-derived mesenchymal stromal cells (UC-MSCs) were seeded (1560 cells / cm2) into a CF4 cell factory system (Nunc) in fibrinogen-depleted culture medium composed of α-MEM (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: Mouse Eklf cDNA was derived by RT-PCR of RNA from DMSO-induced MEL cells and cloned into the vector pCMV-Flag (Invitrogen), resulting in pFlag-EKLF ...
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bioRxiv - Cell Biology 2021Quote: RNA isolated from THP1-derived macrophages and IPF PCLS were reverse-transcribed using Superscript IV Reverse transcriptase (Thermofisher, Cat#18090050) to obtain cDNA for gene expression analysis ...
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bioRxiv - Bioengineering 2022Quote: Cardiomyocytes were derived from iPS cells and cultured in RPMI 1640 media supplemented with B27 and Antibiotic-Antimycotic (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: Human iPSC-derived Brain Microvascular Endothelial-like cells were treated with 25 μg/mL fluorescent transferrin conjugate (Thermo Fisher Scientific) and incubated at 37oC for 30 min ...
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bioRxiv - Cell Biology 2021Quote: NRVMs were derived from 1- or 2-day old Sprague-Dawley rates using the Cellutron Neomyt kit (Thermo Fisher Scientific). Cell purity was routinely >90% cardiomyocytes ...
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bioRxiv - Neuroscience 2022Quote: ... 1 ng/ml brain-derived neurotrophic factor and 10 ng/ml ciliary neurotrophic factor in supplemented Neurobasal medium (ThermoFisher Scientific)) ...
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bioRxiv - Immunology 2022Quote: ... COVID-19 whole blood-derived RNA was extracted from samples collected in Tempus Blood RNA tubes using the Tempus Spin RNA Isolation Kit (ThermoFisher) as described by the manufacturer ...
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bioRxiv - Neuroscience 2020Quote: ... Briefly, cDNA was derived from 5 week-old C57BL/6J retina (Jackson, 000664) following RNA extraction (RNAqueous Kit, Ambion, AM1912) and reverse transcription with Superscript III (ThermoFisher ...
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bioRxiv - Cancer Biology 2020Quote: ... The patient-derived low-passage MM cell lines (a gift from G.-E. Ghanem) were grown in F-10 (Gibco, Invitrogen), supplemented with 10% FBS (Gibco ...
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bioRxiv - Immunology 2020Quote: ... Mass spectrometry derived data was searched against Human RefSeq 81 protein database in Proteome Discoverer 2.1 (Thermo Scientific, Bremen, Germany) using SEQUEST and Mascot (version 2.5.1 ...
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bioRxiv - Neuroscience 2020Quote: hiPSC-derived neuron and astrocyte co-cultures were loaded with Fluo 4 direct (calcium assay kit by Thermo Fisher Scientific) for 30 minutes in 37 °C ...
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bioRxiv - Microbiology 2020Quote: Phosphatidylserine externalization in HAP1-derived cells was quantified by co-staining with both annexin V-Pacific Blue (AnV-PacBlue) (Invitrogen) and propidium iodide (PI ...
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bioRxiv - Cell Biology 2020Quote: Freshly isolated HTII-280+ and HTII-280− cells derived from CD31−CD45−EpCAM+ cells of human distal lungs were resuspended in Advanced DMEM/F12 (Thermofisher) base medium supplemented with 10 mM Hepes (Gibco) ...