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Citations for Thermo Fisher :
751 - 800 of 10000+ citations for Piggybac Transposable Element Derived 3 PGBD3 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... and customized si-KIF4 3′ UTR targeting endogenous KIF4 mRNA 3’-UTR region (5′-GGAAUGAGGUUGUGAUCUUTT-3′) were purchased from Thermo Fisher Scientific.
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bioRxiv - Cell Biology 2020Quote: ... reverse: 5’-GGGGTCGGGAGGAACGG-3’ (Macrogen, South Korea) and beta-actin forward: 5’-CCTGGTCGGTTTGATGTT-3’ and reverse: 5’-GTGCGACGAAGACGA-3’ (Invitrogen, USA). Data analysis was made in CFX ManagerTM Software (BioRad ...
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The skin environment controls local dendritic cell differentiation and function through innate IL-13bioRxiv - Immunology 2021Quote: ... FW 5’-CTCTCTGGGCGAAATCTGCT-3’ and REV 5’-GAGTGCTTTCGCTATGTTGTTCA-3’ for Clmn and FW 5’-TGATGGGTGTGAACCACGAG-3’ and REV 5’-GCCGTATTCATTGTCATACCAGG-3’ for Gapdh using a QuantStudio 7 (Applied Biosystems). Transcript levels are expressed as the ratio of 2−ΔCT (Transcript of interest)/2−ΔCT (Gapdh).
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bioRxiv - Neuroscience 2022Quote: ... total RNA was extracted from freshly-dissected n=3 young (3-month-old) or n=3 aged (18-month-old) zebrafish brain in TRIzol (Invitrogen, 15596). Three independent experimental replicates were used for bulk RNA sequencing ...
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bioRxiv - Cell Biology 2020Quote: ... samples were washed 3 times with PBS and incubated with appropriate fluorescent-labeled secondary antibodies (1:1000 dilution in 0.5% BSA, Life Technologies Inc) and the nuclear marker DAPI (Biolegend) ...
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bioRxiv - Neuroscience 2020Quote: ... Tissue sections were then washed 3× 10 min with PBS and incubated with the appropriate AlexaFluor-conjugated secondary antibody (1:200; Invitrogen/ThermoFisher Scientific) for 2h ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were rinsed 3 times in PBS and incubated for 1 h at room temperature with corresponding secondary antibodies (1:500, Life Technologies). Sections were washed three times with PBS and incubated with DAPI for 5 min (1:5,000 in PBS ...
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bioRxiv - Biochemistry 2020Quote: ... and cleavage products were detected using mouse monoclonal BC-3 antibody which detects aggrecan cleavage at the Glu392↓Ala393 bond (Cat n.: MA316888, Life Technologies). Immobilon Chemiluminescent HRP substrate (Cat ...
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bioRxiv - Neuroscience 2020Quote: ... Dishes were washed 3 times with PBS-Triton X and incubated with Alexa-Fluor 488 conjugated secondary antibody 1:1000 (Molecular Probes-Invitrogen) in dilution buffer for 1 hour at RT ...
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bioRxiv - Neuroscience 2020Quote: ... for 1 h at RT and then incubated for 3 days at 4°C with the monoclonal mouse anti-NeuN antibody conjugated with Alexa-488 (clone A60, Life Technologies) at a concentration of 0.5 µg mL-1 into blocking buffer ...
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bioRxiv - Neuroscience 2020Quote: ... sections were washed 3×15 min in 0.1% PBT and transferred to secondary antibody solutions (goat polyclonals; Thermo Fisher; 1:200) prepared in 0.3% PBT ...
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bioRxiv - Neuroscience 2020Quote: ... Tissue was rinsed 3 times in PBS and incubated with the corresponding secondary antibody: goat anti-mouse Alexa-488 (Invitrogen, A32723); donkey anti-mouse Alexa-647 (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2021Quote: ... Samples were washed 3 x 10 min with PBS/PBS-T and incubated with secondary antibody anti-Rabbit-568 (1:500, Thermofisher #A21069), anti-Rabbit-488 (1:100 ...
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bioRxiv - Immunology 2020Quote: ... Cells were then mixed with 40 μL of phenotype panel containing surface marker antibodies (Supplementary Table 3) and a dead cell marker (LIVE/DEAD Fixable Near-IR; Invitrogen L10119) (final dilution 1/1000) ...
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bioRxiv - Neuroscience 2020Quote: ... tissues were washed in PBS (3×5 min) and incubated with secondary antibodies conjugated to Alexa Fluor 488 (1:400; goat anti–rabbit; Life Technologies) or Alexa Fluor 594 (1:400 ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then washed 3× with PBS and incubated for 1h with the corresponding Alexa Fluor secondary antibodies (Thermo Fisher Scientific) at 1:1000 dilution (in 3% BSA ...
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bioRxiv - Molecular Biology 2022Quote: ... Sections were rinsed 3 times in PBS and incubated for 1 h at room temperature with corresponding secondary antibodies (1:500, Life Technologies). Sections were dry-mounted on slides using Fluoromount (Sigma ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 µg of an HA-tag antibody (MBL, M132-3) was mixed with 20 µL of Dynabeads Protein G (Invitrogen, #10004D) and subsequently added to extracts ...
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bioRxiv - Pathology 2022Quote: ... Sections were washed with PBS and incubated for 3 hrs with a secondary antibody conjugated to Alexa 488 (Invitrogen, Carlsbad, CA). Sections were washed with PBS and mounted with mounting medium containing 1.5 μg/ml DAPI (VECTASHIELD w/DAPI ...
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bioRxiv - Neuroscience 2021Quote: ... neurospheres were washed 3 times with PBS and incubated with secondary antibody goat anti-mouse Alexa Fluor 488 (Thermo Fisher Scientific) diluted at 1:1000 for 45 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... slices were washed in PBS (3 × 15 min) and treated with the secondary antibody (streptavidin-conjugated Alexa Fluor 546 (1:1000, Invitrogen #S11225) or Alexa Fluor 647 (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... Blots were washed 3×10 minutes in PBS-T and incubated with secondary antibody (goat anti-rabbit IgG HRP conjugate, Invitrogen, G21234) diluted in 1% milk +BSA (1:5000 ...
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bioRxiv - Cell Biology 2022Quote: ... Blots were washed 3 times in TBS-T and incubated with secondary antibody (Goat anti-rabbit IgG HRP conjugate, Invitrogen, G21234) for 1 hour at room temp ...
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bioRxiv - Developmental Biology 2020Quote: ... followed by 3 stringent washes in PBS-tween and application of Alexa flour® 596 conjugated goat anti mouse secondary antibody (A11005, Invitrogen) for 1 hour followed by 3 washes in PBS tween ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were subsequently processed with donkey anti-sheep secondary antibody conjugated to Alexa Fluor 488 at 3 µl/ml and (Life Technologies), donkey-anti rabbit secondary antibody conjugated to Alexa Fluor 555 at 5 µl/ml (Life Technologies) ...
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bioRxiv - Neuroscience 2020Quote: ... They were incubated in 3% H2O2 prior and then with an anti-GFP primary antibody (1:1000, Thermofisher, #A-11122; RRID:AB_221569) The sections were subsequently incubated for 3 h at room temperature with a biotinylated anti-rabbit IgG antibody (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were rinsed 3 times in PBS and incubated for 1 h at room temperature with corresponding secondary antibodies (1:500, Life Technologies). Sections were washed three times with PBS and incubated with DAPI for 5 min (1:5,000 in PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... Antibodies (3 μg for ChIP-qPCR and 9 μg for ChIP-seq) were coupled to protein G dynabeads (Thermo Fisher Scientific) for 6 hours at 4°C and then incubated with fragmented chromatin over night at 4°C ...
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bioRxiv - Immunology 2022Quote: ... 700 μg of protein were mixed and rotated with 0.5 μg of STING antibody for 3 h at 4 °C after which 25 μL of Dynabeads Protein G (Invitrogen, 10004D) were added to each sample and rotated for an additional hour ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were then washed with 1X PBS for 3 10-minute washes before applying the secondary antibody goat anti-rabbit Alexa 647 (Invitrogen A21245), which was allowed to incubate at RT for 2 hours ...
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bioRxiv - Neuroscience 2023Quote: ... samples were protected from light going forward and incubated for 5 days at 37 °C in 1.6 ml of PTwH + 3% donkey serum containing a 1:250 dilution of secondary antibody (donkey anti-Rabbit Alexa 647, ThermoFisher Scientific). Samples were washed 5x 5 min and incubated overnight at rt in 2 mL of PTwH.
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bioRxiv - Molecular Biology 2022Quote: ... and slides were washed 3 x 5min in PBS before the application of 1:500 goat α-rabbit AlexaFluor488 antibody (ThermoFisher) for 30min ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were then washed 3 times and incubated with species-specific secondary antibodies conjugated to Alexafluor-680 or -800 (Thermo Fisher) diluted 1:5000 in 2% BSA/TBS-T (0.1% ...
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bioRxiv - Immunology 2024Quote: High-throughput screening of antibodies was done using Expi293 cells grown in 3 mL plates in 24 deep-well plates (Thermo Fisher). Cultures were grown for four days ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed in TBST for 10 minutes 3 times and incubated in Alexa Fluor conjugated secondary antibodies diluted 1:200 (Life Technologies) with DAPI (1:50,000 ...
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bioRxiv - Microbiology 2023Quote: ... All collected fractions (3 ml) were analyzed using SDS-PAGE and Western blotting with an anti-His-tag antibody (ThermoFisher Scientific).
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated at room temperature for 3 h in TBS containing AlexaFluor-546-conjugated donkey anti-goat IgG secondary antibody (1:400, A-11056, Fisher Scientific), 0.5% Triton X-100 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed 3 times with PBS and AlexaFluor (AF488, AF568) conjugated anti-rabbit or anti-mouse antibodies (#A-11008, A-11004 Fisher Scientific) were added and incubated for 1h at room temperature (RT) ...
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bioRxiv - Bioengineering 2023Quote: ... The barrier function of the Calu-3 monolayer was terminally evaluated via immunofluorescence imaging of tight junction protein 1 (ZO-1 antibody, Invitrogen, 339194) and permeability assays using 500 µL of 2 mg/mL FITC-dextran (4 kDa ...
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bioRxiv - Immunology 2023Quote: ... whole-cleared tissues were washed for 3-4h-hours and incubated with a secondary Alexa Fluor™ 647 donkey anti-rabbit antibody (A-31573, Invitrogen) for another 3 days in room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were blocked again and incubated with 2-3 drops of an anti-mouse poly-HRP-conjugated secondary antibody from the Invitrogen Tyramide SuperBoost secondary staining kit (ThermoFisher, B40912). Samples were rinsed three times with 1X PBS and the tyramide working solution conjugated to AlexaFluor 488 was added and allowed to react for 20 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibody incubation was for 3 h with Alexa Fluor 647 goat anti-mouse IgG (1:1000, Thermo Fisher Scientific, A21236) and Alexa Fluor 488 goat anti-rabbit IgG (1:1000 ...
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bioRxiv - Developmental Biology 2022Quote: ... Table 2) incubated for 3 overnights at 4 °C in blocking buffer with Alexa-dye conjugated secondary antibodies (1:500, Molecular Probes) incubated for 1 overnight at 4 °C in blocking buffer ...
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bioRxiv - Microbiology 2023Quote: ... Slides were again washed 3 times with 50 mL of PBS and incubated with anti-mouse IgG-Alexa 488 secondary antibody (1:1000; ThermoFisher A28175) at 37°C for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: ... The cells were then washed with cold 1X PBS three times for 3 min each and incubated with Alexa 555-labeled anti-rabbit and Alexa 488-labeled anti-mouse secondary antibody (Thermo Fisher) at room temperature for 1 hour ...
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bioRxiv - Neuroscience 2023Quote: ... Then cells were washed 3x for 5 min with PBS and incubated for 1 hour at RT with secondary antibodies (Supplementary Table 3) and Hoechst 33342 (Invitrogen, 62249). After 3 washes for 5 min with PBS ...
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bioRxiv - Neuroscience 2023Quote: ... and then incubated with secondary antibody (AlexaFluor 546 donkey anti-mouse for PV, goat anti-rabbit for GluR2/3 or NPY, both 1:400, Thermo Fisher). Secondary antibody was dissolved in 1% normal donkey serum for PV or 1% normal goat serum for GluR2/3 or NPY ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed three times and incubated with Alexa546-anti- rabbit secondary antibody (1:1000 in 3% BSA/PBS, 200 µl/well, Invitrogen, A11035) for another hour at 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... slides or coverslips were washed 3 times for 5 min in PBS and incubated with appropriate species-specific Alexa Fluor-conjugated secondary antibodies (Molecular Probes). DNA was counterstained with Hoechst 33342 (Invitrogen) ...
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bioRxiv - Neuroscience 2024Quote: ... Retinas were washed in 1x PBS for 3×30 minutes at RT and transferred to secondary antibody solution containing 1:1000 goat anti-mouse Alexa 488 (Thermo Fisher Scientific ...