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Citations for Thermo Fisher :
801 - 850 of 10000+ citations for Human Amyloid Beta 42 Abeta42 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... CAS number: 328–42–7), 2-Ketoglutaric acid, disodium salt, dehydrate (>99%, CAS number: 305–72–6) were purchased from Acros Organics, Belgium ...
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bioRxiv - Immunology 2020Quote: ... The recovered bacteria were serially diluted in MH broth and plated onto MH agar plate followed by incubation at 42 °C for 24 h under the microaerophilic conditions in a tri-gas incubator (Thermo Scientific). Bacterial colonies that appeared on the plate were counted to enumerate colony-forming units (CFU) ...
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bioRxiv - Genomics 2020Quote: ... iPSC-derived RPE (iPSC-RPE) cells at 42 days post differentiation were lysed with TRIzol reagent (Thermo Fisher Scientific; cat # 15596026) and total RNA was isolated using the Direct-zol RNA MiniPrep Kit (Zymo Research ...
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bioRxiv - Physiology 2022Quote: ... Primary αSMA rabbit polyclonal antibody (1:500 dilution, Cat # RB-9010-PO, 42 kDa) was purchased from Thermo Scientific (Waltham, MA). GAPDH rabbit polyclonal antibody (1:10,000 dilution ...
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bioRxiv - Molecular Biology 2023Quote: ... in 25 µL of buffer containing 20 mM of NH4Ac at 42 degrees for 2 hours followed by phosphatase treatment using 1 µL of FastAP Thermosensitive Alkaline Phosphatase (ThermoFisher #EF0651) at 37 degrees for 1 hour ...
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bioRxiv - Neuroscience 2023Quote: ... in 6-well tissue culture plates containing 1 ml pre-warmed culture medium (42% MEM, 25% Basal Medium Eagle; Thermo Scientific), 25% Normal Horse Serum ...
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bioRxiv - Biochemistry 2023Quote: ... UV-crosslinked at 120 mJ and blocked for 2 hours rotating at 42°C using ULTRAhyb Oligo hybridization and blocking buffer (Thermo Fisher). Blots were probed rotating overnight at 42°C with 32P-labeled DNA probes prepared as described below ...
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bioRxiv - Biophysics 2024Quote: ... The gel was run in 0.5x TBE buffer at 120 V for 42 min and stained with 1x SYBR™ Gold Nucleic Acid Gel Stain (Invitrogen) diluted with 0.5x TBE buffer for 20 min ...
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bioRxiv - Cell Biology 2024Quote: ... Two sets of 12 100 µL 2.66 ng/ µL enriched resuspended mitochondria aliquots in 200 µL PCR tubes were made per condition and heated on a temperature gradient of 42-64°C (2°C steps) for 5 minutes at each temperature using a Veriti 96-well thermal cycler (Applied Biosystems).
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bioRxiv - Neuroscience 2024Quote: ... Aβ1-42 was diluted to a final concentration of 100 μM by adding DMEM F-12 phenol-red free media (Life technologies) or artificial CSF (ACSF) ...
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bioRxiv - Molecular Biology 2023Quote: The membrane was prewetted with 2x SSC and incubated for >1 hour at 42°C with 10 ml of ULTRAhyb ultrasensitive hybridization buffer (Thermo Fisher). The radiolabeled probe prepared by linear PCR as above was heat denatured for 5 min at 100°C and immediately added to the hybridization buffer ...
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bioRxiv - Biochemistry 2019Quote: ... The reaction was quenched by adding 5mM beta-mercaptoethanol and desalted to remove unreacted dye (ThermoFisher, 7K MWCO). The extent of labelling was quantified by measuring the 595nm/280nm absorbance ratio ...
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bioRxiv - Biochemistry 2021Quote: ... for spike protein or RBD detection and mouse anti-beta tubulin antibody (1:5000; Thermo Fisher; MA5-16308) as a loading control ...
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bioRxiv - Cell Biology 2021Quote: A rat beta cell line (INS1E, AdexxBio C0018009) at passage 38–46 was cultured in RPMI (Gibco 21875) supplemented with 5% (v/v ...
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bioRxiv - Neuroscience 2021Quote: ... The reaction was stopped following the addition of 4x sample buffer (containing 10% beta mercaptoethanol (BME; Thermo Fisher)) to each well and heated to 95°C for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... Western blotting for GATA3 and Beta-Actin was carried out by lysing the cells with RIPA buffer (ThermoFisher) supplemented with benzonase and protease inhibitors ...
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bioRxiv - Cancer Biology 2023Quote: ... Treatment with the vehicle hydroxypropyl-beta-cyclodextrin (HPBCD, Acros Organics; 5% w/w in water for injection (WFI)) ...
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bioRxiv - Microbiology 2020Quote: ... we used human monoclonal antibodies produced recombinantly in human Expi293F cells (Life Technologies) as described before (Fang et al ...
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bioRxiv - Cell Biology 2021Quote: ... anti-human IgG-Alexa647 and anti-human IgG-Alexa568 were obtained from Invitrogen, anti-acetylated tubulin (T7451 ...
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bioRxiv - Cell Biology 2020Quote: ... and human GAPDH and human KIF18A Taqman probes and primers (Thermo Fisher Scientific) were used for reverse transcription and qRT-PCR ...
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bioRxiv - Neuroscience 2020Quote: ... and subjected to human total tau ELISA (human tau: # KHB0042, Thermo Fisher Scientific) according to manufacturer’s instructions.
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bioRxiv - Immunology 2019Quote: ... T cells were isolated using Dynabeads Untouched Human T Cells Kit using manufacturer’s protocols (Thermo Fisher, Waltham, MA). Isolated T cells were kept in T cell media ...
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bioRxiv - Cancer Biology 2019Quote: ... was analyzed using Affymetrix Human Transcriptome Array 2.0 (HTA 2.0) microarrays after cDNA amplification using GeneChip WT Pico Reagent Kit (Affymetrix) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... Total Tau, phospho Tau (pS396, pS199, pT231 and pT181) levels were analysed by Human ELISA Kit from Invitrogen. Cerebral organoids and supernatants (for Aβ ...
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bioRxiv - Cancer Biology 2021Quote: MMP1 in the secretome of cell lines was quantified with a MMP1 Human ELISA Kit (Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: Allogenic CD8+ T-cells were isolated by negative selection using Dynabeads Untouched Human CD8 T Cells Kit (Invitrogen), labeled with carboxyfluorescein succinimidyl ester (CFSE ...
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bioRxiv - Immunology 2019Quote: ... Dynabeads Human T-Activator CD3/CD28 for T-cell expansion and Activation kit (1132D) were purchased from Thermofisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... total RNA were extracted then reverse transcribed using the Ion AmpliSeq Transcriptome Human Gene Expression kit (Thermofisher Scientific). The cDNA libraries were amplified and barcoded using Ion AmpliSeq Transcriptome Human Gene Expression core panel and Ion Xpress Barcode Adapter (Thermofisher Scientific) ...
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bioRxiv - Synthetic Biology 2021Quote: BMP4 concentration released into the media was quantified using the BMP-4 Human ELISA Kit from (ThermoFisher, EHBMP4). Standards and protocols were done according to the instructions of the kit ...
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bioRxiv - Immunology 2021Quote: ... and anti-Spike IgG was quantified using a Human SARS-CoV-2 Spike (Trimer) IgG ELISA Kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Intracellular staining was performed using buffer sets of Human Regulatory T-cell Staining Kit (eBioscience/Thermo Fisher Scientific), following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: Isolation of CD3+ T-cells was performed using Dynabeads® Untouched™ Human T-cells Kit (11344D, Invitrogen). CD3+ T-cells were stained with CellTrace Violet Cell Proliferation Kit (C34571 ...
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bioRxiv - Cancer Biology 2022Quote: ... T cells were isolated from PBMCs using the Dynabeads® Untouched Human T Cells Kit (Invitrogen, Karlsruhe, Germany) following the manufacturer’s protocol.
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bioRxiv - Biochemistry 2022Quote: ... labeled with Alexa Fluor 488 from the Zenon™ Human IgG Labeling kit (Molecular Probes, Eugene, OR, USA) and 15 μg/mL of 7-amino-actinomycin D (7-AAD ...
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bioRxiv - Immunology 2022Quote: ... and CD4+ and CD8+ T cells were further isolated with Dynabeads Human CD4+ or CD8+ Isolation Kits (ThermoFisher), following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: Total tau content was quantified in brain extracts by ELISA (Human Tau (total) ELISA kit (#KHB0041, Invitrogen, UK), see supplementary methods ...
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bioRxiv - Bioengineering 2023Quote: ... The plasma insulin concentration was determined using a Human Insulin ELISA kit according to the manufacturer’s protocol (Invitrogen). The relative bioavailability (RA ...
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bioRxiv - Immunology 2023Quote: ... IL-18 and human IL-1β was analyzed in the cell culture supernatant by commercial ELISA kits (Invitrogen) following the manufacturer’s recommended procedures.
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bioRxiv - Neuroscience 2024Quote: Human fibroblast cells were reprogrammed using the Sendai virus-based CytoTune iPS 2.0 kit (CytoTune 2.0, Invitrogen, A16517). Fibroblasts were plated in six-well plates two days before transduction to achieve a density of 2 × 105 to 3 × 105 cells per well ...
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bioRxiv - Cell Biology 2022Quote: ... 20 gravid hermaphrodites were dissected in a drop of M9 (86 mM NaCl, 42 mM Na2HPO4, 22 mM KH2PO4, and 1 mM MgSO4) on an epoxy slide square (Thermo Fisher Scientific), previously coated with 0.1% poly-L-lysine ...
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bioRxiv - Genomics 2020Quote: ... Ligation reactions were transformed into One Shot™ Stbl3™ chemically-competent E.coli cells (42°C heat-shock, 90 seconds; Invitrogen C737303) and positive clones were selected by Sanger sequencing ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were differentiated for the following 26 days (42 days of total differentiation) in neuronal differentiation media consisting of Neurobasal Media (Thermo Fisher Scientific) + B27 supplement (without Vitamin A ...
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bioRxiv - Bioengineering 2020Quote: ... sections were incubated in the following primary antibodies diluted in PBS containing 1% normal donkey serum: rabbit polyclonal anti-Pax6 (1:200, Invitrogen, 42-6600), mouse monoclonal anti-beta-Tubulin-III ...
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bioRxiv - Genomics 2021Quote: ... The suspension was filtered through a 42-μm nylon mesh and stained with the intercalating fluorochrome propidium iodide (PI, Thermo Fisher Scientific) and treated with RNase II A (Sigma-Aldrich) ...
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bioRxiv - Immunology 2022Quote: ... This transfer plasmid (500 ng) was transformed using heat shock (42 °C, 1 min) into competent DH10 BAC cells (Thermo Fisher Scientific). Cells were cultured on agar plates containing selection antibiotics and Blu-gal ...
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bioRxiv - Neuroscience 2021Quote: ... This reaction was incubated for 60 minutes in triplicates for each temperature (37°C, 40°C and 42°C) and terminated by adding one volume of sample buffer (NuPAGE LDS; Invitrogen, Carlsbad, CA) containing 5% of 2-β-mercapto-ethanol ...
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bioRxiv - Genomics 2021Quote: ... The suspension was filtered through a 42-μm nylon mesh and stained with the intercalating fluorochrome propidium iodide (PI, Thermo Fisher Scientific) and treated with RNase II A (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2022Quote: ... Extracted DNA was further purified using the ethanol precipitation protocol.42 The quantity and quality of the resulting DNA extractants were evaluated using a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific) as well as by gel electrophoresis.43 All the DNA samples were stored at -20 °C before further processing.
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bioRxiv - Bioengineering 2023Quote: ... extension: 68 °C for 42 secs and total number of cycles: 35) in ProFlex 3×32-well PCR system (Applied Biosystems, USA). Desired amplified product was excised from 1.2% agarose gel and purified using GeneJET gel extraction and DNA cleanup kit following supplier’s protocol (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... carinii+Rattus norvegicus or P.murina+Mus musculus UniProt database (42; accessed 06/2020) using Proteome discoverer v2.4 and the Sequest HT search algorithm (Thermo Scientific, Waltham, MA). Proteomic analysis was performed on 3 independent EV isolations and data was concatenated for coverage of the proteome ...