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Citations for Thermo Fisher :
701 - 750 of 10000+ citations for Human Amyloid Beta 42 Abeta42 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Luminex assays were performed using the following kits according to the manufacturer’s instructions: hCG Human ProcartaPlex Simplex Kit (Invitrogen, EPX010-12388-901), Bio-Plex Pro Human Inflammation Panel 1 IL-28A / IFN-λ2 (Bio-rad ...
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bioRxiv - Genetics 2021Quote: ... and then the quantification was conducted using the Quantifiler Human DNA Quantification Kit (Thermo Fisher Scientific) on an Applied Biosystems 7500 Real-time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: Human genomic DNA was extracted using the PureLink Genomic DNA Mini Kit (Thermo Fisher Scientific, USA) or QIAamp DNA Blood Mini Kit (QIAGEN ...
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bioRxiv - Microbiology 2020Quote: ... supernatants were collected and cytokine levels assed with Human IFNγ ELISA Kit (ThermoFisher Scientific, Vienna, Austria) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... The subclass of each IgG antibody was determined using a human IgG subclass ELISA kit (Invitrogen).
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bioRxiv - Bioengineering 2022Quote: ... Anti-human IL6 Ab concentrations were quantified using the Quant-it protein assay kit (Life Technologies). Ab conjugates were validated on 10% TBE urea denaturing gels and validated on an agarose gel (Supplementary Figure S8) ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were isolated using a commercial kit (MagniSort Human CD4+ T Cell Enrichment; Affymetrix) and infected by incubation for 4 h at 37°C with 156,250 or 2,500 TCID50 (50% tissue culture infectious dose ...
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bioRxiv - Bioengineering 2022Quote: ... CD8+ T cells were positively enriched by Magnisort Human CD8+T cell positive selection Kit (Invitrogen). Nuclear fraction was isolated using NE-PER Nuclear and Cytoplasmic extraction kit (ThermoFisher) ...
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bioRxiv - Bioengineering 2019Quote: ... RNA from human cells was cleaned and collected utilizing an RNAqueous-Micro Kit (Ambion, cat. #AM1931) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... the human IL-2 ELISA kit was used in accordance with manufacturer’s instructions (Thermo Fisher Scientific). Murine IL-2 ELISA’s were performed using the mouse IL-2 ELISA kit (Thermo Fisher Scientific).
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bioRxiv - Immunology 2021Quote: Allogenic CD8+ were isolated by negative selection using Dynabeads Untouched Human CD8 T Cells Kit (Invitrogen) and labeled with carboxyfluorescein succinimidyl ester (CFSE ...
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bioRxiv - Neuroscience 2023Quote: ELISA was performed on total and SEC fractions using Tau (Total) Human ELISA Kit (KHB0041, Invitrogen) by following the directions provided by the manufacturer ...
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bioRxiv - Immunology 2023Quote: ... The supernatant was collected on day 3 and analyzed by human IL-2 ELISA Kit (ThermoFisher) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2020Quote: ... and VIC labelled human β actin endogenous control probe (Human - 4326315E) or RNA28S5 (Human - Hs03654441_s1) (Thermo Fisher Scientific) so that amplified mRNA can be normalised to β actin or RNA28S5 ...
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bioRxiv - Neuroscience 2021Quote: ... Human recombinant fibroblast growth factor (human FGF2) (10 ng/ml, Invitrogen) was added to Dulbecco’s Modified Eagle’s Medium (DMEM)/F-12 (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... Human T cells were activated with human CD3/CD28 Dynabeads (Invitrogen), at a bead:cell ratio of 2:1 and transduced using supernatant collected from Phoenix-AMPHO cells ...
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bioRxiv - Cancer Biology 2023Quote: ... Antibodies used for human samples were anti-human CD31 (#17031942, Invitrogen), anti-human CD45 (#17945942 ...
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bioRxiv - Biochemistry 2020Quote: ... was codon-optimized and cloned into a pFastBac dual vector(42) for production of baculovirus by the Bac-to-Bac method (Invitrogen). High Five Cells (Thermofisher ...
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bioRxiv - Molecular Biology 2019Quote: ... followed by a digestion with the Nuclease and Enhancer proteins at 42°C before being electrophoresed on a 10% TBE Gel (Thermofisher). The gel was then stained in 100 ml 1x TBE buffer with 2 μl of 10 mg/ml ethidium bromide for 5 min ...
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bioRxiv - Genetics 2019Quote: ... anti-CD127-FITC and (30 µl/ml, clone eBioRDR5, Cat. no.11-1278-42, Thermo Fisher Scientific, Waltham, U. S.) and anti-CD25-PE (80 µl/ml ...
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bioRxiv - Immunology 2021Quote: Total RNA was isolated from 3 × 106 Xela DS2 (passages 28, 29 and 31) and Xela VS2 (passages 42, 46 and 48) using TRI reagent (Invitrogen) according to the manufacturer’s instructions with the following modifications ...
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bioRxiv - Immunology 2020Quote: ... and anti-CD127-PE-Cy5 (clone eBioRDR5; 5 μL; cat. # 15-1278-42) all from Thermo Fisher (Supplementary Fig. 4). mAbs for chemokine receptors (i.e ...
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bioRxiv - Cell Biology 2021Quote: ... were transformed into DH10EmBacY chemically competent cells with heat shock at 42°C for 15 seconds followed by incubation at 37°C for 5 hours in S.O.C media (Thermo Fisher Scientific). The cells were then plated on LB-agar plates containing kanamycin (50 μg/ml) ...
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bioRxiv - Cell Biology 2021Quote: ... RNA released from the permeabilized tissue and captured by the DNB was reverse transcribed overnight at 42℃ using SuperScript II (Invitrogen, 18064-014 ...
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bioRxiv - Cancer Biology 2020Quote: ... The membrane was incubated overnight at 42°C with probe in hybridization buffer (50% formamide, 5X SSCPE, 5X Denhardt’s solution (Invitrogen 750018), 0.1 mg/ml salmon sperm DNA (Agilent 201190) ...
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bioRxiv - Plant Biology 2021Quote: ... Released RNA from permeabilized tissues was captured by the DNB and reverse transcribed overnight at 42 °C using SuperScript II (Invitrogen, 18064-014 ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was crosslinked using a UV stratalinker and blocked at 42°C using ULTRAhyb Oligo hybridization and blocking buffer (Ambion) for 2 hours while rotating ...
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bioRxiv - Microbiology 2019Quote: ... Membranes were hybridized at 42 °C with 5 nM of a 5’-biotinylated oligonucleotide probe (Table S4) in ULTRAhyb Ultrasensitive Hybridization Buffer (Ambion) and then washed according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... was carried out overnight (∼18-20h) at 42°C by incubating permeabilized tissue with 75 µl cDNA synthesis mix containing 1X First strand buffer (Invitrogen), 5 mM DTT ...
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bioRxiv - Immunology 2020Quote: Livers were harvested 40-42 hours after sporozoite challenge and total RNA was extracted following homogenisation using TRIzol (ThermoFisher Scientific). cDNA was generated using the RETROScript Kit (Ambion) ...
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bioRxiv - Cancer Biology 2020Quote: ... was carried overnight (∼18-20h) at 42°C by incubating permeabilized tissue with 75 µl cDNA synthesis mix containing 1X First strand buffer (Invitrogen), 5 mM DTT ...
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bioRxiv - Microbiology 2021Quote: ... RT-qPCR was performed as previously described(42) using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher, Cat#4444436) and a OneStepPlus Real-Time PCR System (96-well format ...
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bioRxiv - Neuroscience 2019Quote: ... RAI1 antibodies were generated by immunizing rabbits with a synthesized RAI1 peptide (aa 28 to 42, ENYRQPGQAGLSCDR, Thermo Fisher Scientific), followed by affinity purification using the peptide as the affinity ligand (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... 130-090-51) was from Miltenyi. FOXP3 (PCH1.1) (dilution: 1/20; Cat. Number: 53-4776-42) was from Thermo Fisher eBioscience ...
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bioRxiv - Immunology 2022Quote: ... cDNA was synthesized using 1 ug of RNA reverse transcribed for 50 min at 42°C using oligo(dT) primer (IDT) and SuperScript II RT (Invitrogen). For qPCR analysis ...
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bioRxiv - Molecular Biology 2023Quote: ... 32P-labelled oligonucleotides (listed in Table S4) were hybridized overnight at 42°C in ULTRAhyb®-Oligo hybridization buffer (Ambion). After hybridization ...
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bioRxiv - Genetics 2023Quote: ... cells were dissociated with accutase and stained with SSEA1 eFluor 660 antibody 1:100 (Thermo Fisher Scientific, 50-8813-42) at 4 °C for 30 minutes on ice ...
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bioRxiv - Neuroscience 2023Quote: hiPSC-derived neurons after 38-42 days of terminal differentiation were transfected with pCAG-MaxGFP (GFP) using Lipofectamine 2000 (Invitrogen). The GFP-labeled neurons were imaged at 10x magnification using a Nikon C2 confocal fluorescence microscope ...
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bioRxiv - Molecular Biology 2023Quote: ... The blot was incubated for 2 h at 42 °C in hybridization buffer (ULTRAhyb™ Ultrasensitive Hybridization Buffer [Invitrogen; AM8669]). The probe was added to hybridization buffer and hybridized to the blot overnight at 38 °C ...
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bioRxiv - Biophysics 2023Quote: ... Applying currents in the 0-1000 mA range allowed the application of forces spanning from 0 to 42 pN using Dynabead M270 streptavidin-coated superparamagnetic beads (Invitrogen).
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bioRxiv - Cancer Biology 2024Quote: ... Dnmt3L KO clones (QKO) were generated by transfecting TKO J1 mESC (42) with sgRNA-containing px458 using Lipofectamine 3000 (Invitrogen) and sorting GFP+ cells after 72h ...
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bioRxiv - Molecular Biology 2023Quote: ... Stock probes were diluted 1:1000 in RNA FISH hybridization buffer preheated to 42°C (15% deionized formamide, 10% dextran sulfate, 2× SSC from Ambion, 0.02% BSA ...
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bioRxiv - Biochemistry 2024Quote: ... 8 µL of 2.5X MaP Buffer was added and incubated at 42°C for 2 minutes before addition of 1 µL SuperScript II Reverse Transcriptase (ThermoFisher) and incubation at 42°C for 3 hours ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were stained for 30 min on ice with pre-optimized concentrations of the following fluorochrome-conjugated antibodies: AREG-APC (17-5370-42; Invitrogen), CD49f (ITGA6)-PE (561894/555736 ...
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bioRxiv - Neuroscience 2024Quote: ... RNA released from the permeabilized tissue and captured by the DNB was reverse transcribed overnight at 42°C using SuperScript II (Invitrogen, 18064-014 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Sections were stained with an anti-LAMP1-A488 antibody (HuO9, SAOS2: anti-hLAMP1, 1:250, Invitrogen, #53-1079-42; HC1954: anti-mLAMP1, 1:250, Invitrogen, #53-1071-82 ...
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bioRxiv - Cell Biology 2024Quote: ... was reverse transcribed in a total volume of 20 µl for 1 h at 42°C using 0.4 µg of random hexamer primers and 200 U of reverse transcriptase (Thermo Scientific) in the presence of 20 U of ribonuclease inhibitor (Thermo Scientific) ...
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bioRxiv - Cell Biology 2019Quote: ... In immunofluorescence and western blot study we used the following antibodies: Beta-actin (Thermofisher cat no. MA515739) Beta Tubulin (BT7R ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated overnight with primary Cholera Toxin beta polyclonal antibody (rabbit; 1:1000, RRID: AB_779810, Invitrogen) diluted in PBS-T and for approximately 1h30 with donkey antirabbit secondary antibody AlexaFluor647 (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... In immunofluorescence and western blot study we used the following antibodies: Beta-actin (Thermofisher cat no. MA515739), Anti Alpha Tubulin Antibody Clone DM1A (Thermofisher cat no-62204) ...