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Citations for Thermo Fisher :
801 - 850 of 10000+ citations for 7 Benzyl 2 7 Diazaspiro 3.5 nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... desalted through Zeba Spin Columns (MW cut-off 7 kDa, Thermo Scientific) and analyzed by immunoblotting (Figure S1B) ...
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bioRxiv - Neuroscience 2023Quote: ... Then the thoraxes were embedded in 7% SeaKem LE agarose (Fisher Scientific) which was hardened at 4°C for 60 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... Reactions were run using Quantstudio 7 Real-Time PCR system (Applied Biosystems), and subsequently analyzed for copy number using CopyCaller software (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2022Quote: ... and cultured for 7 days in RPMI-1640 Glutamax (Gibco; A14517-01) supplemented with 10% heat-inactivated FBS ...
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bioRxiv - Cell Biology 2023Quote: ... on a QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems). Rp49 was used as a housekeeping gene ...
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bioRxiv - Developmental Biology 2023Quote: ... 7 μm sections were generated by cryohistology (NX70 cryostat Thermo Fisher Scientific), using methods previously described (45) ...
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bioRxiv - Cell Biology 2023Quote: ... Hydrogel solutions were titrated to ∼pH 7 using sodium bicarbonate (Gibco, 25080094) with pH indicator strips (Merck ...
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bioRxiv - Genomics 2023Quote: 7×106 cells were dissociated into single cells with Accutase (Thermo Fisher) at 37°C for 8 -10 minutes ...
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bioRxiv - Cancer Biology 2023Quote: qPCR was performed according to the three-step method in the protocol from APExBIO HotStart [APExBIO K1070] in a ViiA 7 Real-Time PCR System (ThermoFisher). initial denaturation (hold ...
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bioRxiv - Microbiology 2023Quote: ... Cells were passaged every 6-7 days applying 0.5 mM EDTA (Thermofisher) in sterile Dulbecco’s Phosphate Buffered Saline (DPBS ...
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bioRxiv - Developmental Biology 2023Quote: ... The resulting cDNA was utilized for quantitative qPCR (Viia 7; Applied Biosystems). cDNA was diluted 1:50 in H20 and mixed with 10μM primers and PowerSYBR Green PCR Master Mix (Applied Biosystems ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were cultured for 7 days in medium containing HAT Supplement (Gibco) followed by 4 days in HT Supplement (Gibco ...
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bioRxiv - Neuroscience 2023Quote: ... These were mixed with a solution containing 7 µl Lipofectamine 3000 (Invitrogen) in 250 µl OptiMEM ...
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bioRxiv - Biophysics 2023Quote: ... with an QuantStudio™ 7 Flex Real-Time PCR System (ThermoFisher Scientific). Data analysis was performed as described previously84.
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bioRxiv - Developmental Biology 2023Quote: Samples were loaded and run in a 7% Tris-acetate gel (Invitrogen) in tris-acetate buffer on ice at 45V ...
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bioRxiv - Cancer Biology 2023Quote: Cells were cultured in RPMI-1640 (Raji, Daudi, Ramos, MCF-7) (Gibco) or DMEM (E0771 ...
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bioRxiv - Genetics 2023Quote: ... 0.15 M NaF using Zeba 7 kDa spin desalting columns (ThermoFisher Scientific) then diluted to approximately 50 µM in the desalting buffer ...
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bioRxiv - Cell Biology 2023Quote: Real-time qPCR was performed on ViiA™ 7 system (Applied Biosystems). Total volume of qPCR reaction was 20µl and it contained 10µl of 2x qPCR Master Mix Low ROX (Eurogentec) ...
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bioRxiv - Neuroscience 2024Quote: ... Detector values were captured using Chromileon 7 software (Dionex, Thermo Fisher Scientific) to quantify peak height ...
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bioRxiv - Molecular Biology 2024Quote: ... and 7 were developed using Super Signal ECL reagent (Thermo Fisher Scientific) and imaged with a ChemiDoc Gel Imaging MP System (Bio-Rad) ...
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bioRxiv - Immunology 2023Quote: ... Apoptosis was assessed using the CellEvent Caspase-3/7 Detection Reagent (Invitrogen) over TCB-treatment duration or at specific intervals ...
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bioRxiv - Microbiology 2023Quote: ... and ran on a ViiA 7 Real-Time PCR System (ThermoFisher Scientific), with a 15 sec 95 °C denaturation step and a 1 min 60 °C annealing/extension step for 40 cycles ...
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bioRxiv - Genomics 2023Quote: ... Lysate was partitioned into two tubes and 7 µL RNaseI (Invitrogen, AM2249) was added and incubated for 1 h at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... and 7 μm sections were placed onto Superfrost Plus slides (Fisher Scientific). Tissue sections were processed for routine histology and stained with hematoxylin and eosin reagents (Sigma-Aldrich St ...
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bioRxiv - Bioengineering 2023Quote: ... and 0.05 % D-Glucose monohydrate (Thermo Scientific Chemicals; CAS 14431-43-7) in 500 mL Erlenmeyer flasks without baffles ...
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bioRxiv - Cancer Biology 2024Quote: ... using the QuantStudio 3 and 7 Real-Time PCR systems (Applied Biosystems).
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bioRxiv - Developmental Biology 2019Quote: ... or by using a 1:2 ratio of benzyl alcohokbenzyl benzoate followed by mounting in Permount (Fisher Scientific) on microscope slides for imaging.
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bioRxiv - Immunology 2021Quote: ... 1:100 human IgG (1 mg/ml) as FcR block and 2 % FCS using the following staining reagents: 7-AAD 1:400 (Thermo Fisher Scientific), CD19-BV786 1:20 (clone SJ25C1 ...
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bioRxiv - Microbiology 2021Quote: ... ATPase activity was assayed by a continuous spectrophotometric method using a 2-amino-6-mercapto-7-methylpurine ribonucleoside–purine nucleoside phosphorylase reaction to detect released inorganic phosphate (EnzChek kit; Thermo Fisher Scientific) (62) ...
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bioRxiv - Molecular Biology 2022Quote: ... HCA-7 and MDCK cells were allowed to differentiate for 7 days after plating at 100% confluency in the presence of 2 µg/mL doxycycline (Fisher Scientific AAJ6042203) when inducing transgene and/or reporter transcript expression ...
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bioRxiv - Microbiology 2021Quote: PBMCs were plated into a 10 cm dish at 7×107 cells/dish and adhered for 2 hours in AIM-V (Gibco-BRL, CA). The nonadherent cells were removed gently and frozen as the source of lymphocytes for co-culture use ...
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bioRxiv - Immunology 2022Quote: The production of NO was evaluated using 4-amino-5-methylamino-2’,7’-difluorofluorescein diacetate (DAF-FM DA)(D23844; Invitrogen, Waltham, MA). Following leukocyte isolation ...
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bioRxiv - Systems Biology 2019Quote: Determination of intracellular levels of total ROS was carried out by flow cytometry using 2’,7’-dichlorodihydrofluorescein diacetate (DCH2FDA, Thermo Fisher Scientific), as previously described (Amara et al ...
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bioRxiv - Microbiology 2020Quote: Amount of ROS in dissected guts (from 10 animals) was estimated using 2’,7’-Dichlorodihydrofluorescein diacetate (H2DCFDA, C6827, ThermoFisher Scientific, MA, USA), following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: The compound 5-(and-6)-carboxy-2’,7’-dichlorodihydrofluorescein diacetate (carboxy-H2DCFDA, Image-iT LIVE Green Reactive Oxygen Species Detection Kit, Molecular Probes; Invitrogen, I36007) was used to visualise the in vivo production of ROS ...
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bioRxiv - Microbiology 2021Quote: ... Cells were incubated with mouse mAb SARS-CoV-2 nucleocapsid antibody (SinoBiological, 1:100) and rabbit Claudin 7 polyclonal antibody (ThermoFisher, 1:200) overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Staining was performed on ice for 25 minutes in PBS with 2 % FCS using the following antibodies: 7- AAD 1:400 (Thermo Fisher Scientific), CD19-BV786 1:20 (clone SJ25C1 ...
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bioRxiv - Bioengineering 2022Quote: ... Cultures were washed three times with washing solution and then incubated for 30 min at room temperature on the OrganoFlow (7°, 2-min intervals) with Alexa Fluor 488 (#a32731; Invitrogen; 1:250) and Alexa Fluor 647 (#a31571 ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then washed three times with unsupplemented neurobasal media and loaded with 200μM 2’,7’-dichlorofluorescein diacetate (DCFH-DA) fluorescent dye (Thermo Fisher Scientific, MA) for 30 minutes at 37°C ...
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bioRxiv - Plant Biology 2022Quote: ... Mock-treated and infected roots of WT and HvMPK3 KO plants were incubated in 30 μM 2’,7’-dichlorodihydrofluorescein diacetate (CM-H2DCFDA; Cat no. C6827, Invitrogen™, USA) and PI (1 mg.ml-1 ...
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bioRxiv - Microbiology 2023Quote: ... Cell pellets were washed and re-suspended in PBS buffer free of KCl and incubated in the dark with 25-μM of 2’,7’-dichlorodihydrofluorescein diacetate (CM-H2DCFDA) suspended in dimethylsulfoxide (DMSO) for 60 min at room temperature (Molecular Probes, Invitrogen). After incubation ...
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bioRxiv - Neuroscience 2023Quote: Larval zebrafish at 4–7 days post-fertilization were anesthetized (0.025% MS-222, Syndel) and mounted in 2% low melting point agarose (Thermo Fisher Scientific 16520), dorsal-up ...
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bioRxiv - Bioengineering 2022Quote: The level of reactive oxygen species was assessed in the homogenate supernatant of RPE and retina tissue with using 2′–7′-dichlorofluorescein diacetate (DCFH-DA; ThermoFisher Scientific Inc.) molecular probes ...
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bioRxiv - Molecular Biology 2023Quote: ... The iPSCs were then cultured for 7 days in Neurobasal/DMEM-F12 medium (1:1 v/v) containing 2% B27 (Gibco, 17054–044), 1% N2 (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were then dried at 100°C and derivatized with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (Acros Organics, ThermoFisher Scientific) prior to reverse-phase HPLC (Agilent 1100 series ...
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bioRxiv - Cell Biology 2024Quote: ... The flow-through was then dried in a speed-vac and derivatized with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (Acros Organics, ThermoFisher Scientific) prior to reverse-phase HPLC (Agilent 1100 series ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred to nitrocellulose membrane at 20 V for 7 minutes using the iBlot™ 2 Transfer Stack (Thermo Fisher Scientific). Membranes were blocked in 5% non-fat milk powder in TBS+0.1% Tween (TBST ...
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bioRxiv - Cancer Biology 2021Quote: ... in a ViiA 7 or 7900 HT Real-Time PCR system (Applied Biosystems). Data analysis was performed using the RQ Manager (Applied Biosystem ...
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bioRxiv - Molecular Biology 2020Quote: ... MCF-7 passage cell line were prepared using 0.05% trypsin solution (Invitrogen, Carlsbad) and seeded in 96 well tissue culture plates ...
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bioRxiv - Cell Biology 2020Quote: ... purified CD14+ monocytes were differentiated for 7 days in RPMI-1640 medium (GIBCO) supplemented with 10% Fetal Bovine Serum (FBS ...