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Citations for Thermo Fisher :
801 - 850 of 10000+ citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPENTANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... Haematopoietic stem cells differentiated into BMDMs by incubating for 7 days in BMDM medium (1:1 SFM:DMEM, Gibco) supplemented with 10 % FCS (Biochrom) ...
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bioRxiv - Developmental Biology 2023Quote: ... Aggregation medium (BSA supplemented N2B@7 media) consisted in 500ml 1:1 mix of Neurobasal (Invitrogen 21103-049) and DMEM/F12 (Invitrogen 21331) ...
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bioRxiv - Developmental Biology 2022Quote: ... and incubated with Alexa-Flour-488 or 555 secondary antibodies (1:1000) along with 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen, Camarillo, CA, 1:1000) for 1 hr at room temperature (RT) ...
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bioRxiv - Cell Biology 2022Quote: ... 1:200 O/N at 4 °C) and Alexa Fluor 594-conjugated anti-mouse IgG antibody (#A11005, Invitrogen, 1:2000 for 2 hours RT). Nuclei staining was done in 0.5 μg/mL Hoechst 33324 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... 2.0µM Template Switching Oligo (TSO) (5′-Biotin-AGAGACAGATTGCGCAATGNNNNNNNNrGrGrG-3′; IDT) and 2U µl−1 of Maxima H Minus reverse transcriptase (Thermo Fisher Scientific, #EP0752). Plates were quickly centrifuged before incubation at 42°C for 90min ...
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bioRxiv - Immunology 2021Quote: ... Samples from Biosafety Level 3 were inactivated for 2 hours at RT with 4% paraformaldehyde (ThermoFisher Scientific) after extracellular and intracellular staining.
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bioRxiv - Microbiology 2022Quote: ... adding 3 mL HPLC grade methanol and 2 mL HPLC grade chloroform (C297-4, Thermo Fisher Scientific), then shaking at 22°C for 1 hr ...
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bioRxiv - Cell Biology 2023Quote: ... DAPI (4’,6-Diamidino-2-henylindole, dihydrochloride) was obtained from Invitrogen (Catalog: D1306, CAS:28718-90-3).
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bioRxiv - Genomics 2023Quote: ... and CRISPRmap library plasmid (2:3:4 ratio by mass) using Lipofectamine 3000 (Thermo Fisher Scientific L3000001) in lentiviral packaging medium (Opti-MEM (Thermo Fisher Scientific 31-985-070 ...
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bioRxiv - Biochemistry 2021Quote: ... A DMSO solution with ≈4–7 mol pyrene-iodoacetamide (Molecular Probes) per mol of actin was added to the F-actin mix ...
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bioRxiv - Biochemistry 2023Quote: ... AMC (7-amino-4-methylcoumarin) fluorescence reference standard (ThermoFisher Scientific, A191): Prepare a 100 mM stock in DMSO ...
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bioRxiv - Cell Biology 2024Quote: ... and CellTracker Blue (4-chloromethyl- 6.8- difluoro- 7- hydroxycoumarin; CMF2HC; Invitrogen), respectively ...
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bioRxiv - Cell Biology 2020Quote: ... and then incubated for 2 h at room temperature with the following primary antibodies: rabbit anti-EEA1 (1:3000, Thermo Fisher Scientific Cat# PA1-063A ...
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bioRxiv - Developmental Biology 2020Quote: ... the ovaries were incubated for 2 h at room temperature with secondary Alexa 568-conjugated antirabbit IgG (1:500 in PBX; Invitrogen, Life Technologies), washed and counterstained with Hoescht (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... 0.5% Triton X-100 in PBS) for 2 h at room temperature and then with primary antibody (rabbit anti-GFP, 1:1000, Invitrogen, G10362) diluted in blocking buffer overnight at 4℃ ...
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bioRxiv - Molecular Biology 2019Quote: ... Membranes were washed with TBST and incubated for 1 h in TBST/2% BSA with secondary antibody (Immunopure goat anti-mouse # 31430, Thermo Scientific, and peroxidase-labeled affinity anti-rabbit # 04-15-06 ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were then washed and incubated at room temperature for 2 h in donkey anti-mouse alexa fluor 594 (1:1000, Life Technologies) and donkey anti-rabbit alexa fluor 488 (1:1000 ...
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bioRxiv - Neuroscience 2019Quote: ... before incubation (2 h at room temperature) with a mixture of AlexaFluor 488 or 568 goat anti-rabbit secondary (1:500, Life Technologies) and biotinylated horse anti-mouse secondary (1:500 ...
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bioRxiv - Molecular Biology 2019Quote: ... and then for 1 h at room temperature in 2 µg/mL Alexa 488-conjugated donkey anti-rat antibody (A-21208, Thermo Fisher). Slides were mounted with ProLong Gold containing DAPI (P36931 ...
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bioRxiv - Immunology 2021Quote: ... Triton X-100 with 5 % normal-goat-serum and unconjugated F(ab′)2 goat anti-mouse IgG (H+L) antibody (1:500, Thermo Scientific), at 4 °C for 2 h ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed twice in PBS then suspended in goat anti-mouse IgG (H&L) cross-adsorbed secondary antibody Alexa Fluor 488 (2 mg mL−1; Thermofisher Scientific) in PBS 0.1 % BSA for 45 min at 4 °C ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated in blocking solution for 2 h at room temperature with fluorescent donkey anti-goat alexa fluor 488 (1:500, Invitrogen, A11055) and goat anti-rabbit alexa fluor 647 (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were incubated for 2 h at RT in the secondary antibody (1:1000, goat anti mouse Alexa Fluor 647, Life Technologies) carrier solution (1x PBS ...
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bioRxiv - Neuroscience 2020Quote: ... sections were washed three times in PBS – 0.05% saponin and incubated for 2 h with a donkey anti-goat Alexa Fluor® 488 (1/1000, Invitrogen A11055) diluted in PBS – 0.05% saponin – 0.2% BSA ...
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bioRxiv - Neuroscience 2021Quote: ... and rinsed and then incubated for 2 h with appropriate Alexa Fluor-conjugated secondary antibodies (R37117, Thermofisher, Waltham, MA, 1 : 500). Fluorescent images were acquired using Olympus IX81 or Zeiss 880LSM microscopes.
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bioRxiv - Neuroscience 2020Quote: ... The sections were then washed three times in 0.1 M PBS and incubated for 2 h at room temperature in donkey anti-goat AlexaFluor546 (1:500, Invitrogen, #A-11056), donkey anti-goat FITC (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... they were incubated for 2 h at RT with an Alexa Fluor 555 nm-conjugated goat anti-mouse secondary antibody (1:500; Molecular Probes, ThermoFisher Scientific cat#A21127 ...
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bioRxiv - Immunology 2020Quote: ... in PBS/0.5% BSA at room temperature for 2 h and washed in PBS/0.2% Tween-20 at room temperature for 1 h five times before mounting with SlowFade Diamond Antifade Mountant (S36963, Life Technologies).
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 2 h in blocking/permeabilization buffer with goat anti-rabbit AlexaFluor 488 (Invitrogen, Carlsbad, CA; 1:500).
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bioRxiv - Evolutionary Biology 2021Quote: ... (diluted 1:400 in 2% BSA) and a second step of 1 h of incubation with an Alexa Fluor 488 conjugate secondary antibody (Molecular Probes) (diluted 1:1000 in 2% BSA) ...
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bioRxiv - Molecular Biology 2022Quote: C2C12 cells were plated onto 6-well plates at a 2 x105 density and 24 h later transfected with either negative control siRNA (Negative Control No.1 siRNA, Life Technologies) or custom siRNAs targeting S1P (Silencer Select siRNAs ...
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bioRxiv - Cell Biology 2023Quote: ... the glasses were incubated for 2 h at room temperature with secondary antibodies (1:800 Alexa Fluor 594 and 647 antibodies from Molecular Probes) and mounted in polyvinyl alcohol mounting medium with DAB-CO [52] ...
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bioRxiv - Microbiology 2023Quote: ... cells were incubated for 2 h at RT with AlexaFluor (AF) 488-labeled donkey anti-rabbit antibody (1:500, Invitrogen, Poland) and AF647-labeled phalloidin (1:400 ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated with the following fluorophore-conjugated secondary antibodies for 2 h at room temperature: anti-rabbit Alexa Fluor 488 (1:400, goat, Invitrogen, A11008), anti-rabbit Alexa Fluor 568 (1:400 ...
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bioRxiv - Developmental Biology 2023Quote: ... Sections were then incubated with secondary antibodies at room temperature for 2 h at 1:500 dilution: anti-rabbit AF594 (Invitrogen, A21207); anti-rat AF488 (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... washed 3 times with PBS and incubated with the following secondary antibodies at RT for 2 h: Alexa Fluor 488-conjugated goat anti-mouse IgG (1:200; Molecular Probes) and Alexa Fluor 633-conjugated goat anti-rabbit IgG (1:1,000 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed in PBS and incubated for 2 h at room temperature with secondary antibodies: goat anti-rat Alexa Fluor 555 (1/500; Invitrogen, #A21434), goat anti-rabbit Alexa Fluor 488 (1/500 ...
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bioRxiv - Physiology 2023Quote: ... followed by incubation for 2 h at room temperature with Alexa Fluor 680-linked anti-mouse IgG (A21058; Invitrogen, 1:5000). Signals were visualized with an Odyssey Infrared Imaging System (LI-COR Biosciences) ...
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bioRxiv - Immunology 2024Quote: ... followed by 2 h incubation with HRP-conjugated secondary antibody (1:10000; anti-mouse or anti-rabbit, Invitrogen #31430 and #31460) in TBST ...
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bioRxiv - Developmental Biology 2024Quote: ... sections were incubated for 2 h with corresponding secondary antibodies: Alexa Fluor 488 goat anti-mouse IgG (1:800, A32723 Thermo Fisher), Alexa Fluor 568 goat anti-mouse IgG (1:800 ...
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bioRxiv - Plant Biology 2024Quote: ... Immunolabelling experiments were performed with LM19 and LM20 (1:10 dilution in TTBS-milk, 2 h incubation) followed by anti-rat IgG-Alexa488 (Invitrogen®) or anti-rat IgG alkaline phosphatase conjugate (Sigma-Aldrich A8438 ...
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bioRxiv - Biophysics 2021Quote: ... The cells were then washed three times with PBS containing 1% BSA followed by 1 h incubation with donkey anti-mouse Alexa Fluor 488-conjugated secondary antibody (1:1000, ThermoFisher) at 4°C in the dark ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were washed and incubated with fluorescent secondary antibodies diluted in 1% BSA in PBS for 1 h (1:400; anti-mouse-Alexa488, Invitrogen A21202 ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were washed and incubated with fluorescent secondary antibodies diluted in 1% BSA in PBS for 1 h (1:400; anti-mouse-Alexa488, Invitrogen A21202; anti-rabbit-Alexa568, Invitrogen A10042 ...
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bioRxiv - Cell Biology 2020Quote: ... 1:200) was performed for 1 h at 37°C in combination with 1 μM HCS cell mask (Invitrogen; H32720) that stains the entire cell ...
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bioRxiv - Cell Biology 2022Quote: ... slices were labelled with SPY650-DNA (1:1000, #SC501, Spirochrome) for 1 h and CellTraceTM CFSE (1:1000, #C34554, ThermoFisher) for 30 min ...
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bioRxiv - Cell Biology 2022Quote: ... The primary antibody was added at a ratio of 1:100 and incubated for at least 1 h at room temperature while the secondary antibody (1:300 Invitrogen) was allowed to incubate with the sample for 30 min at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... Cryosections were blocked with 1% bovine serum albumin in PBS for 1 h at room temperature and incubated with primary antibodies (anti-perilipin 1, PA5-72921, 1:200, ThermoFisher, Waltham ...
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bioRxiv - Molecular Biology 2023Quote: ... Secondary antibodies diluted in 3% BSA/PBST were incubated with the samples at 37°C for 1 h: goat anti-rat IgG (H+L) (Thermo Fisher Scientific A11006, 1:100), goat anti-rabbit IgG (H+L ...
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bioRxiv - Molecular Biology 2023Quote: ... for 4 h in Opti-MEM (31985-070; Gibco). The cells were cultured in DMEM for 56 h.