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Citations for Thermo Fisher :
651 - 700 of 10000+ citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPENTANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... overnight at 4°C and further stained with 1:200 Alexa Fluor 633 Goat anti-Rabbit IgG (H+L) Secondary Antibody (Invitrogen A21071) at a 20°C for 3 hours ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were incubated for 4 h at room temperature in fluorescent conjugated secondary antibodies (Alexa Fluor 488 goat anti-mouse (Th; 1:500; Invitrogen; A10667) and Alexa Fluor 594 goat anti-rabbit (DsRed ...
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bioRxiv - Cell Biology 2019Quote: ... then further cleared by centrifugation at 4°C and 25658 g for 1 h (Thermo Fisher Scientific, #A23-6×100 rotor). The supernatant was passed through a 0.22 µm filter (Corning #431097 ...
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bioRxiv - Immunology 2021Quote: ... cells were fixed for 1 h at 4°C using a FOXP3/Transcription Factor Staining Buffer Set (Invitrogen, for FOXP3 detection) or 15 min at room temperature using a 1.85% formaldehyde solution in PBS (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were washed five times with the same blocking solution and then incubated with the corresponding secondary antibodies conjugated with Alexa fluorophores at 4°C with gentle agitation for 24 h (1:1000, Molecular Probes). Finally ...
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bioRxiv - Bioengineering 2023Quote: ... To minimize nonspecific binding the samples were blocked for 4 h at room temperature with a blocking buffer containing 1% of Tween20 (Fisher Scientific) and 3 % of BSA (VWR ...
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bioRxiv - Cell Biology 2023Quote: ... the supernatants of lysed cells were incubated in shake tubes over 1 h at 4°C with Pierce™ Protein G magnetic beads (Invitrogen) previously attached to V5 tag antibodies (R960-25) ...
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bioRxiv - Molecular Biology 2023Quote: ... was equilibrated with lysis buffer and then incubated with the cell lysate at 4 °C for 1 h on an end-to-end rotator (Cat# 88881001, ThermoFisher scientific). The slurry was transferred to a 15 ml falcon tube ...
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bioRxiv - Immunology 2023Quote: ... was incubated overnight at 4°C and Alexa Fluor 488 goat anti-chicken IgY (H+L) secondary antibody (1:100, A-11039, ThermoFisher Scientific) was applied to detect GFP+ cells ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then rinsed three times for 10 min in PBS and incubated for 24 h at 4°C in secondary antibodies (donkey anti-rabbit Alexa Fluor 488, 1:250, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Iba-1 (1:1000, Wako) and COX-2 (1:1000, Thermo Fisher) at 4°C overnight ...
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bioRxiv - Neuroscience 2021Quote: ... Nucleus staining was performed using 4’,6-diamidino-2-phenylindole (DAPI) (3 mM, D3571, Molecular Probes). Cells were counted from four randomly selected fields per culture under a confocal microscope (TCS SP8 ...
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bioRxiv - Biochemistry 2021Quote: ... 2-[6-(4’-hydroxy) phenoxy-3H-xanthen-3-on-9-yl] benzoate (HPF) from Molecular Probes® and Horse radish peroxidase (HRP ...
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bioRxiv - Cell Biology 2019Quote: 1.5 cm-thick intact human brain slice (Number 7, see Fig. S3) was randomly chosen and passively incubated with 400 ml TO-PRO-3 (T3605, Thermo Fisher, 1:2000 dilution) in PBS at room temperature for 1 week ...
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bioRxiv - Immunology 2019Quote: ... the CellEvent Caspase-3/7 Green Detection Reagent (Thermo Fisher Scientific) was added to the cells with complete media for 30 min in 37°C and the caspase-3/7 positive cells were measured on an Attune NXT flow cytometer (Life Technologies ...
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bioRxiv - Cancer Biology 2019Quote: ... CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit (Invitrogen) was used according to the manufacturer’s protocol with modest modifications ...
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bioRxiv - Cancer Biology 2020Quote: ... caspase-3 and caspase-7 green apoptosis-assay reagent (Life Technologies) was added to the culture medium following manufacturer’s instructions ...
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bioRxiv - Zoology 2021Quote: ... and CellEvent Caspase-3/7 Green ReadyProbes Reagent (ThermoFisher Scientific, R37111) (CellEvent ...
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bioRxiv - Immunology 2024Quote: ... CellEvent™ Caspase-3/7 Green Detection Reagent (Thermo Fisher, C10423) was added to cultures at 20 μM and incubated for 30 min at 37°C ...
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bioRxiv - Systems Biology 2020Quote: ... Alkylated proteins were digested by Lys-C (1:100 w/w) (WAKO, Richmond, VA, USA) at 37°C for 3 h and trypsin (1:100 w/w) (Thermo Fisher Scientific) at 37°C for 16 h ...
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bioRxiv - Cell Biology 2020Quote: ... cells were washed once with PBS and incubated at 37°C for 1 h with ONPG solution (3 mg/mL (Fisher Scientific, 34055) + 0.05% NP-40 ...
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bioRxiv - Microbiology 2022Quote: ... RNA samples were reverse transcribed with first strand synthesis primed with RT-primer 1 to 3 with a Maxima H Minus First Strand cDNA synthesis kit (Thermo Scientific, USA) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... slices were washed and incubated 3 h at RT in secondary antibodies donkey-α-rabbit DyLight 650 (1:500, SA5-10169, Thermo Fisher) and donkey-α-mouse Alexa 568 (1:500 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Fecal samples were immediately transferred to an anaerobic chamber within 2 h after defecation and suspended at 20% (w/v) in reduced peptone water (0.1%, pH 7; Thermo Fisher Diagnostics AG, Pratteln, Switzerland). Immobilization of 10 ml fecal slurry in polymer gel beads (gellan gum (2.5% ...
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bioRxiv - Developmental Biology 2022Quote: ... To-Pro-3 (1:500, Invitrogen T3605). Secondary antibodies were obtained from Jackson ImmunoResearch or Invitrogen and used at 1:200.
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bioRxiv - Cell Biology 2019Quote: RNF168 si #1: 5’- GGCGAAGAGCGAUGGAGGATT-3’ (Ambion)
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bioRxiv - Biochemistry 2022Quote: ... 3) 1% Pluronic-127 (cat. # P6866, ThermoFisher) in BRB80 ...
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bioRxiv - Systems Biology 2022Quote: ... diluted 1:3 with DPBS (Life Technologies) and 25μL added to the wells ...
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bioRxiv - Bioengineering 2021Quote: ... + 1 × 10−3 M sodium pyruvate (Gibco) + 0.05 × 10−3 M 2-mercaptoethanol (Sigma)] supplemented with soluble anti-mouse CD28 (5 µg/ml ...
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bioRxiv - Neuroscience 2020Quote: ... and Toto-3 (1:1000; Thermo Fisher) as nuclear counterstain.
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bioRxiv - Immunology 2022Quote: ... ICOS-SB436 (ISA-3, Invitrogen, 1:50), IgG-BV480 (goat polyclonal ...
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bioRxiv - Developmental Biology 2023Quote: ... or TO-PRO-3 (1:50000, ThermoFisher) for 1 hour at room temperature or overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... proteinase K (3 μg ml−1; ThermoFisher) digestion ...
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bioRxiv - Immunology 2023Quote: ... claudin-3 (1:200; Thermo Fisher Scientific), claudin-15 (1:200 ...
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bioRxiv - Molecular Biology 2023Quote: ... 1-3 L of Expi293F cells (ThermoFisher) were grown at 37°C to a cell density of 3.2×106 cells/mL in FreeStyle expression medium (ThermoFisher) ...
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bioRxiv - Bioengineering 2024Quote: ... and TO-PRO-3 (1:5000, ThermoFisher). The combination of Nucred Dead 647 and TO-PRO-3 has previously been described by Dekkers et al24 ...
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bioRxiv - Genetics 2023Quote: ... diluted 1:3 in 1X PBS (Gibco). Trypsin was inactivated by cell media.
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bioRxiv - Cancer Biology 2024Quote: ... 3% H2O2 (Fisher Scientific 7722-84-1) was used ...
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bioRxiv - Immunology 2020Quote: ... Staining was performed on ice for 25 minutes in PBS with 2 % FCS using the following antibodies: 7- AAD 1:400 (Thermo Fisher Scientific), CD19-BV786 1:20 (clone SJ25C1 ...
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bioRxiv - Bioengineering 2022Quote: ... Cultures were washed three times with washing solution and then incubated for 30 min at room temperature on the OrganoFlow (7°, 2-min intervals) with Alexa Fluor 488 (#a32731; Invitrogen; 1:250) and Alexa Fluor 647 (#a31571 ...
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bioRxiv - Bioengineering 2023Quote: ... 4 µM ethidium homodimer-1 and 1 µg ml-1 Hoechst 33342 (Thermofisher, 62249) was added to chondrocyte-laden alginate hydrogels and was incubated for one hour ...
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bioRxiv - Immunology 2022Quote: ... Organoids were then washed with PBS and then incubated for 1-2 h at room temperature with Alexa Fluor-conjugated secondary antibodies (Invitrogen). Organoids were washed again with PBS and mounted in Vectashield (Vector Laboratories ...
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bioRxiv - Neuroscience 2021Quote: ... slices were incubated with secondary antibodies in vehicle for 2 h at room temperature (goat-anti-rabbit Alexa 546, 1:500, ThermoFisher A-11035 ...
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Neurotransmission and neuromodulation systems in the learning and memory network of Octopus vulgarisbioRxiv - Neuroscience 2021Quote: ... Samples were then incubated for 2 h with anti-rabbit Alexa Fluor 594 secondary antibody (1:500; Thermo Fisher Scientific). Slices were washed and mounted on microscope slides ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then rinsed in PBS and incubated (2 h) with secondary antibodies anti-rabbit or anti-mouse Alexa488 (1:1000, Invitrogen), and/or anti-rabbit or anti-mouse CY3 (1:1000 ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Sections were then incubated for 2 h at room temperature with anti-mouse Alexa 488 (Invitrogen A-1101, 1:500) and anti-rabbit Cy3 (Invitrogen A-10520 ...
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bioRxiv - Neuroscience 2020Quote: ... the free-floating sections were incubated for 2 h in staining solution together with anti-rat Alexa 594 and anti-rabbit Alexa 647 (1:400, ThermoFisher). Slices were washed 3 times with PBS and mounted using Dako Fluorescent mounting medium (Agilent) ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 mM DTT) at 37°C for 2 h followed by incubation with 0.75 U UDG (EN0361, Thermo Fisher Scientific) in 1x UDG buffer at 37°C for 45 min ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were washed again in PBS and incubated for 2 h at room temperature in secondary antibody in carrier solution (donkey anti-goat 647, 1:1000, ThermoFisher; donkey anti-rabbit 405 ...
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bioRxiv - Microbiology 2021Quote: ... Cells were incubated for 1 h with primary/detection SARS-CoV-2 anti-RBD rabbit polyclonal antibody (Sinobiologicals) and then 1 h with secondary anti-rabbit HRP-conjugate antibody (Invitrogen). Foci were visualised using TrueBlue™ Peroxidase Substrate (Sera Care ...