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Citations for Thermo Fisher :
8201 - 8250 of 10000+ citations for Recombinant Human HUS1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Pierce Protein A/G Magnetic Beads (Thermo Fisher) and either mouse anti-human MCL-1 (BD Pharmingen 559027 ...
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bioRxiv - Systems Biology 2024Quote: ... BCA protein assay (Thermofisher Scientific, Waltham, MA; 23225) was conducted to normalize protein concentrations between all samples and hexokinase activity was measured according to the manufacturer’s protocol (Abcam ...
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bioRxiv - Developmental Biology 2024Quote: ... 1 ng TrueCut Cas9 Protein v2 (Invitrogen, A36497), and 125 pg myc-GFP mRNA ...
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bioRxiv - Cancer Biology 2024Quote: ... Pierce BCA protein assay (Thermo Fisher Scientific Inc.) was performed for protein quantification ...
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bioRxiv - Molecular Biology 2024Quote: ... BenchMark™ Pre-stained Protein Ladder (Invitrogen #10748010) was used.
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bioRxiv - Immunology 2024Quote: ... protein A/G agarose beads (20422; Thermo Scientific) were incubated together with the lysate with anti-sesn2 antibody for 3 hours at 4°C on a rotor.
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bioRxiv - Immunology 2024Quote: ... Protein concentration was determined by Nanodrop (Fisher Scientific), and proteins were aliquoted ...
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bioRxiv - Molecular Biology 2024Quote: ... 50 µl of Dynabeads protein G (Invitrogen, 10004D) were aliquoted and placed on a magnet ...
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bioRxiv - Molecular Biology 2024Quote: ... for HA-ChIP or Protein G (Invitrogen, 10003D) for H3K9me2-ChIP were added and incubated for 4 hours at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... Seeblue2 plus protein ladder (LC5925, Thermo Fisher Scientific) and samples were loaded onto 10-20 % Tricine SDS-PAGE gels (EC6625BOX ...
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bioRxiv - Molecular Biology 2024Quote: ... alongside a BSA protein standard (Thermo Fisher Scientific), according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... antibody was bound to DynabeadsTM Protein G (Invitrogen) according to producer protocol ...
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bioRxiv - Biochemistry 2024Quote: ... PageRuler Plus Prestained Protein Ladder (Thermo Fisher, 26616) was used to estimate the molecular sizes of bands ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentration was determined with BCA assay (Thermofisher).
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bioRxiv - Genomics 2024Quote: 50 µl of protein A Dynabeads (10001D, Invitrogen) were used in 10 MOWChIP assays for each tissue sample ...
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bioRxiv - Genomics 2024Quote: ... and anti-Flag (Sigma)] and 15 µl Dynabeads protein G (Fisher Scientific) was added to the soluble fraction ...
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bioRxiv - Genomics 2024Quote: ... Protein G Dynabeads (60 µl, Thermo Fisher 10003D) were added for 1hr then beads washed three times in ice cold IP buffer ...
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bioRxiv - Cell Biology 2024Quote: ... 5% CO2 on Coating Matrix Kit Protein (Gibco) coated flasks ...
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bioRxiv - Cell Biology 2024Quote: ... 50µL of Dynabeads Protein G (ThermoFisher Cat#10004D) were added to each sample and the samples were incubated overnight at 4C ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein detection was performed using iBright CL1000 (Invitrogen). Obtained images were analyzed using the ImageJ 1.54 (National Institutes of Health) ...
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bioRxiv - Biochemistry 2021Quote: ... All RNA solutions were prepared by solubilizing in nuclease free water in the presence of RNAseOUT™ recombinant ribonuclease inhibitor (Thermo Fisher, Cat. #. 10777019) at a final concentration of 0.4 U/μL ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then co-transfected with recombinant plasmid (300 ng) together with miR-544-3p mimic or negative control mimic (50 nM) using Lipofectamine 3000 (Thermo Fisher Scientific, Waltham, MA) and Lipofectamine RNAimax (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... at a bead-to-cell ratio of 1:1 in the presence of recombinant IL-4 (Fisher Scientific, #204-IL-010, 10 ng/ml) and anti-IFN-γ antibody (10 μg/ml) ...
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bioRxiv - Microbiology 2019Quote: ... Purified cells were cultured in a tissue culture flask containing 20 mL RPMI 1640 supplemented with recombinant murine Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) (10 ng/mL) (BioSource, Invitrogen cytokines & signaling, Camarillo, CA). At day 3 of the culture ...
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bioRxiv - Cell Biology 2020Quote: ... Recombinant adenoviral vectors were produced and purified using HEK293A cells according to manufacturer’s protocol (ViraPower Adenoviral Expression System; Invitrogen, AdenoPACK 20, Vivapure; Sartorius AG).
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bioRxiv - Cell Biology 2020Quote: ... Clarified lysates were then incubated with 20 μg of recombinant glutathione S-transferase (GST)-RBD-MICAL-L1 and Pierce™ Glutathione magnetic agarose beads (Life Technologies-Invitrogen, cat# 78601) for 2 hrs at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... were prepared from 10 to 11-day-old chicken embryos (SPF eggs, VALO, Cuxhaven, Germany) using recombinant trypsin (Tryple TM, Thermo Fisher Scientific, Planegg, Germany) and maintained in VP-SFM medium ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were replated onto glass coverslips at day 12 of the differentiation in N2/B27 medium supplemented with four recombinant growth factors at 25ng/ml (BDNF; ThermoFisher, NT3, NGF, GDNF; Peprotech). CHIR90221 was included for 4 further days ...
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bioRxiv - Cell Biology 2024Quote: Organoids were treated from day 4 through day 11 for EN1 and day 7 through day 11 for EPC2 with or without 10 ng/mL recombinant IL-13 (200-13, Thermo Fisher Scientific, Waltham, MA), reconstituted in phosphate-buffered saline (PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... and protein lysates were subjected to electrophoresis through SDS–PAGE or 4-12% NuPage Bis-Tris protein gels (Thermo Fisher Scientific) followed by blotting onto nitrocellulose membranes ...
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bioRxiv - Molecular Biology 2020Quote: ... Then proteins were separated by electrophoresis in 4%–12% Bis-Tris Protein Gels and transferred to polyvinylidene difluoride membrane (Thermo Scientific). The membranes were blocked in 5% dry milk in 0.1% Tween-20 in PBS and detected with the indicated antibodies ...
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bioRxiv - Cell Biology 2020Quote: Protein concentration of cell lysates was determined using the Pierce™ BCA protein assay kit according to manufacturer’s instructions (ThermoFisher Scientific). Samples were subsequently subjected to SDS-PAGE and blotted on a nitrocellulose membrane ...
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bioRxiv - Cell Biology 2020Quote: ... forebrain samples were homogenized with lysis buffer and protein concentrations determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Equal amounts of total protein were incubated with TX antibodies (generated in our lab[23] ...
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bioRxiv - Cell Biology 2020Quote: ... and 10 µg of total protein was loaded into NuPAGE™ 4-12% Bis-Tris Protein Gels (Thermo Fisher Scientific, #NP0321BOX). Proteins were transferred to a nitrocellulose membrane using the Trans-Blot® Turbo™ RTA Mini Nitrocellulose Transfer Kit and Trans-Blot© Turbo™ Transfer System (Bio-Rad) ...
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bioRxiv - Cancer Biology 2020Quote: ... The supernatant was stored at −20°C for protein quantification performed using Pierce ™ BCA Protein Assay Kit (Thermo Fisher, 23227).
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bioRxiv - Cell Biology 2020Quote: ... the sample was recovered from the Amicon tube into a new microfuge tube and protein concentration was determined using bicinchoninic acid (BCA) protein assay (Thermo Scientific). Proteins were subjected to overnight digestion with mass spec grade Trypsin/Lys-C mix (1:25 enzyme/substrate ratio) ...
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bioRxiv - Genetics 2021Quote: ... Supernatant was transferred to new a 1.5 microcentrifuge tube on ice and protein quantification was performed using a Qubit protein assay kit (Thermo Fisher Q33212) and a Qubit 4 fluorometer (Q33238).
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bioRxiv - Immunology 2020Quote: ... Lysate volumes were subsequently adjusted for equal protein levels following BCA assay (23227, Pierce BCA protein assay kit, Thermo Fisher, USA), and GTP-bound Rac1 levels were assessed according to the manufacturers protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... The amount of ATP was then determined and normalized with the protein level measured by BCA protein assay (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2021Quote: ... and then 10 μg of protein per sample was mixed with SDS and reducing reagent and loaded onto NuPAGE Bis-Tris protein gels (Invitrogen, NP0322) after boiling at 70 °C for 10 min ...
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bioRxiv - Molecular Biology 2021Quote: ... Final proteins concentration in the NE extracts was determined using the colorimetric Pierce BCA protein assay kit (Thermo Scientific, Waltham, MA). Pellets were stored at −80° C.
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bioRxiv - Molecular Biology 2021Quote: Eluted proteins were separated on a 1.5 mm NuPAGE™ Novex™ 4-12 % Bis-Tris protein gel (Thermo Fisher Scientific) until the running front had migrated roughly 2 cm ...
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bioRxiv - Molecular Biology 2021Quote: ... The eluted proteins were analyzed on a NuPAGE 4-12% Bis-Tris 1.0 mm Mini Protein Gel (Invitrogen, cat. No. NP0321) and stained with Instant Blue (Abcam ...
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bioRxiv - Genomics 2020Quote: ... diluted chromatin was incubated overnight at 4°C with Protein A and Protein G Dynabeads (ThermoFisher Scientific 10002D, 10004D; 25µL each) and indicated antibodies (Key Resources Table) ...
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bioRxiv - Bioengineering 2020Quote: ... the final working concentration of protein was found to be 100 μg/ml using a BCA Protein Assay kit (Thermo Scientific). Purity of the protein was confirmed by SDS-PAGE followed silver staining of the gel ...
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bioRxiv - Plant Biology 2020Quote: ... Total crude protein was isolated from 100 mg of plant leaves using P-PER plant protein extraction kit (Thermo scientific: 89803) and the western blot was carried out by electro blotting the 20µg of total crude protein onto polyvinylidene fluoride (PVDF ...
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bioRxiv - Neuroscience 2019Quote: Synaptosomes were prepared using the Procedure for Synaptic Protein Extraction from Neuronal Tissue” and Syn-PER Synaptic Protein Extraction Reagent (Thermo Fisher, #87793 ...
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bioRxiv - Cancer Biology 2019Quote: ... the protein sample was reacted with biotin-alkyne using the Click-iT Protein Reaction Buffer Kit (Cat. no. C10276, Thermo Scientific) following protocols in the instruction sheet ...
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bioRxiv - Immunology 2020Quote: ... Lysates were boiled and sonicated (15 cycles of 30s on/30s off) and protein concentrations determined by EZQ® Protein Quantitation Kit (Invitrogen). Lysates were then alkylated with iodoacetamide (IAA ...
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bioRxiv - Biophysics 2019Quote: ... the concentration of virus was estimated as we have reported previously (15) by measuring the viral protein concentration using a BCA protein concentration assay kit (ThermoFisher Scientific) and then dividing the measured protein concentration by the average total protein molecular weight per viral particle (1.8 × 108 Da ...