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Citations for Thermo Fisher :
8451 - 8500 of 10000+ citations for Recombinant Human HUS1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: Total proteins from intestinal epithelial cells or intestinal organoids were extracted with M-PER Mammalian Protein Extraction Reagent (Thermo Fisher Scientific) with proteinase inhibitor (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... For normalization total protein concentration was measured on an aliquot of the cell lysate with the Pierce BCA protein kit (Thermo Fisher) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 μg of antibodies against Piwi and Aubergine were each coupled to 50:50 of protein-A and protein-G Dynabeads (Life Technologies). Lysates were incubated with bead-coupled antibodies ...
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bioRxiv - Molecular Biology 2022Quote: ... Antibodies were captured by 20 µ l of a 50:50 mix of Protein-A and Protein-G Dynabeads (Life Technologies). The beads were washed several times sequentially in the buffers containing low salt (150 mM NaCl) ...
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bioRxiv - Cell Biology 2023Quote: ... samples were collected by centrifugation (11,000 ×g, 20 min) and their protein content was determined (Thermo Pierce BCA Protein Assay kit, ThermoFisher, Cat# 23225). Proteins (∼30 µg/lane ...
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bioRxiv - Developmental Biology 2023Quote: ... Protein lysate from each sample was collected and the concentration of the protein was estimated using Pierce™ BCA Protein Assay Kit (ThermoFisher). For each sample ...
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bioRxiv - Immunology 2023Quote: ... Bound immune complexes were captured with an equal mix of protein A and protein G Dynabead magnetic beads (Thermo Fisher Scientific), which recognize all mouse IgG subclasses ...
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bioRxiv - Genetics 2023Quote: ... The concentration of the liberated soluble protein was assessed with the Pierce BCA protein assay kit (Thermo Fisher, Waltham, MA, USA), and equal amounts of protein (180-250 µg ...
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bioRxiv - Immunology 2023Quote: ... Protein concentration of the supernatant was determined by following manufacturers instruction for the Pierce BCA Protein Assay Kit (ThermoFisher-Scientific #23227). Once total protein concentration was determined ...
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bioRxiv - Biochemistry 2023Quote: Purity of all proteins was verified by SDS-PAGE and concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Scientific).
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bioRxiv - Biochemistry 2023Quote: Purity of all proteins was verified by SDS-PAGE and concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Scientific).
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bioRxiv - Molecular Biology 2023Quote: The accumulation of DNA damage response proteins to chromatin following IR-induced DNA damage was examined using the Subcellular Protein Fractionation Kit (Thermo Fisher) as previously described (23) ...
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bioRxiv - Plant Biology 2023Quote: ... The homogenate was centrifuged and the protein concentration of the supernatant was measured using a Pierce 660 nm Protein Assay Reagent (Thermo Scientific). Thirty-five microgram of protein was loaded in each lane ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were centrifuged at 20,000 x g for 10 min at 4 °C and protein concentrations were determined using Pierce BCA Protein Assay Kit (ThermoFisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Chromatin was immunoprecipitated by adding 10µl of both protein A and protein G-conjugated paramagnetic beads (Invitrogen #1001D and #1003D, respectively) and incubated at 4°C for 1h ...
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bioRxiv - Genomics 2023Quote: ... Then the supernatant was collected and the protein concentration was measured using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Rabbit polyclonal anti-HMGB2 (1:1,000 ...
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bioRxiv - Biophysics 2023Quote: ... To immunodeplete Npm2 from Xenopus egg extract purified polyclonal Npm2 antibody (rabbit a-Npm2 IgG, 39) were first cross linked to Protein A beads (Dynabeads™ Protein A, 10001D, ThermoFisher) using Dimethyl pimelidate dihydrochloride (D8388 ...
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bioRxiv - Neuroscience 2023Quote: ... We obtained 40-80 μg of histone proteins per extraction and loaded 20 μg of protein onto a 4-12% Bis-Tris Plus mini gel (Invitrogen# NW04120BOX). Gels were run for ∼20 minutes at 200V using the Invitrogen mini gel tank with Blot MES SDS Running buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were harvested and lysed prior to splitting each sample into two aliquots for D-2-HG quantification and total protein quantification using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were centrifuged at 13,000 rpm for 10 min at 4°C and the protein concentrations were quantified using Pierce BCA Protein Assay Kit (Thermo Fisher). Equal amounts of protein were separated on 8% SDS-PAGE and then transferred onto nitrocellulose membranes (Thermo Fisher) ...
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bioRxiv - Bioengineering 2023Quote: Protein contents of the SEC fractions were measured using a BCA protein assay kit (23225, Pierce, Thermo Fisher Scientific, MA, USA). A standard curve (range 0–1000 μg/ml ...
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Volumetric Compression Shifts Rho GTPase Balance and Induces Mechanobiological Cell State TransitionbioRxiv - Biophysics 2023Quote: ... A small aliquot of cell lysate was later thawed and used for total protein quantification following the “BCA Protein Assay Kit” manual (Thermo Scientific). The same quantity (60 μL ...
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bioRxiv - Molecular Biology 2023Quote: ... The cell lysate was then sonicated and centrifuged at 16,000g for 15min and the supernatants were extracted for protein concentration measurement by Pierce BCA protein assay (ThermoFisher, Cat# 23227). For the samples of differentiation time course ...
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bioRxiv - Cancer Biology 2024Quote: ... The supernatant was transferred to a new tube before determining protein concentration by means of BCA protein assay (Pierce, Thermo Fisher). Protein aliquots were stored at −30 °C until use.
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bioRxiv - Molecular Biology 2024Quote: ... The tissue lysate was centrifuged at 16,000 x g for 20 min and supernatant was extracted for protein concentration measurement by Pierce BCA protein assay (ThermoFisher, # 23227). Each amount of protein was loaded into 8-16% Mini-PROTEIN TGX Precast Protein Gels (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... before membranes were dried overnight 2 h at room temperature and proteins stained with Sypro Ruby Protein Blot Stain (ThermoFisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Lysates were spun down at 10,000 RPM and supernatant collected for protein quantification using a Pierce™ BCA Protein Assay Kit (ThermoFisher-23225). NuPAGE™ 4% to 12% ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples of the resuspended protein and the streptavidin bead-bound protein were prepared in NuPAGE LDS Sample Buffer (Thermo Fisher Scientific) supplemented with ꞵ-mercaptoethanol (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... Sample protein content was determined with the Pierce detection assay and 200-800 µg of proteins were loaded on Dynabeads Protein G for Immunoprecipitation (ThermoFisher Scientific); the beads were previously incubated for 10 min on a wheel at room temperature with PBS-Tween-20 0.01% supplemented or not with 2.5-5 µg of MIC60 ...
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bioRxiv - Biochemistry 2024Quote: ... Efficacy of the process and final protein purity was assessed by SDS-PAGE using NuPAGE 4-12 % Bis-Tris Midi Protein Gels (ThermoFisher Scientific) and mass spectrometry ...
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bioRxiv - Molecular Biology 2024Quote: Protein was isolated from cells as described (Rüegsegger et al., 2001) and quantified by BCA protein assay (#23225, Thermo Fisher Scientific). 40 µg of protein was separated on 10% surePAGE Bis-Tris gels in MOPS running buUer (#M00665 ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were normalized to total worm extract protein using Pierce™ BCA Protein Assay Kit (Thermo Scientific™; catalog no. 23225). Free fatty acid content were determined by measuring absorbance at 570 nm using BMG Labtech CLARIOstar Plus plate reader.
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bioRxiv - Molecular Biology 2024Quote: ... the lysates were centrifuged at 16,400 ×g for 15 min at 4 °C and the protein concentration in the supernatants was measured using a Pierce BCA protein assay kit (ThermoFisher Scientific). Total protein (10 µg ...
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bioRxiv - Molecular Biology 2024Quote: ... The EPR signal intensities were normalized to the protein concentrations of the samples as determined by BCA protein assay kit (Thermofisher ScientificTM).
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bioRxiv - Microbiology 2024Quote: ... for standard protein analysis or to native PAGE using a NativePAGETM 3‒12% mini protein gel and a Sample Prep Kit (Thermo Fisher) for analysis of the heptamerization of PA28γ ...
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bioRxiv - Molecular Biology 2024Quote: ... The lysate was cleared by centrifugation at 21 130 x g at 4 °C for 30 min and protein content quantified using the Pierce BCA protein assay kit (ThermoFisher Scientfic). Twenty µg of the lysate ...
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bioRxiv - Cell Biology 2024Quote: ... The obtained protein lysate was subsequently used for Co-IP of the Prm2-Septin12 protein complex by Dynabeads Protein G Immunoprecipitation kit (10007D, Invitrogen, USA). Magnetic beads were conjugated with anti-protamine 2 antibody (Mab-Hup2B ...
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bioRxiv - Genetics 2024Quote: ... protein levels were normalized by total protein amount in the same lane as determined by Novex Reversible Membrane Protein Stain (Thermo Scientific) performed prior to blocking.
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bioRxiv - Cell Biology 2024Quote: ... The instrument was calibrated by addition of 15 µl of protein standards from Native Mark Unstained Protein Standard (Thermo Fisher LC0725) in buffer A (10 mM Tris-HCl pH 7.4 ...
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bioRxiv - Synthetic Biology 2024Quote: ... Protein concentrations of the soluble cellular protein and 100x concentrated secreted protein samples were quantified against Bovine Serum Albumin (BSA) standards using the Pierce Coomassie Protein Assay kit (#23236, ThermoFisher Scientific). The assays were performed in 96-well transparent plates containing 150 μl of diluted protein sample or 0 ...
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bioRxiv - Synthetic Biology 2024Quote: ... We purchased B-PER™ Complete Bacterial Protein Extraction Reagent and Pierce™ 660 nm Protein Assay Reagent from Thermo Fisher Scientific (USA) ...
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bioRxiv - Cell Biology 2024Quote: General protein synthesis levels were measured using the Click-iT Plus OPP Alexa Fluor 488 Protein Synthesis Assay Kit (Invitrogen, C10456). After 48 h of siRNA transfection ...
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bioRxiv - Neuroscience 2024Quote: ... supernatants were removed and then protein pellets were dissolved in mammalian protein extraction reagent (M-PER, Thermo Fisher Scientific, Waltham, MA), containing 1% Halt Protease and Phosphatase Inhibitor cocktail and 1% EDTA (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... and/or crude mitochondrial fractions were first normalized for total protein content via Pierce BCA Protein assay (Thermo Fisher Scientifc 23225). Samples were resolved by SDS-PAGE and transferred to nitrocellulose membranes ...
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bioRxiv - Synthetic Biology 2021Quote: ... CD3+ T cells were activated with anti-human CD3/CD28 magnetic Dynabeads (CTS, ThermoFisher) in a 1:1 ratio with 100 U mL-1 of IL-7 and 10U mL-1 IL-15 (R&D Systems ...
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bioRxiv - Cancer Biology 2021Quote: ... FAM-labeled target primer and endogenous human ACTB control (beta actin) (Thermo Fisher Scientific) were mixed with master mix and template ...
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bioRxiv - Microbiology 2020Quote: ... Human embryonic stem cell (hESC; H9)-derived neural stem cells (NSC) (Thermo Fisher Scientific) were cultured ...
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bioRxiv - Microbiology 2020Quote: A549 human epithelial cells (ATCC ref# CCL-185) were maintained in F12K media (Gibco) supplemented with 1x GlutaMax (Gibco ...
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bioRxiv - Cancer Biology 2021Quote: ... AlexaFluor 488 conjugated anti-human/mouse CD44 antibody (1:50 dilution, Molecular probes, A25528) was directly added into the cell culture medium of the cells to be stained ...
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bioRxiv - Cell Biology 2020Quote: ... Taqman probes targeting human GAPDH (Hs02758991_g1) and IFIT1 (Hs03027069_s1) were purchased from Life Technologies. qRT-PCR data were collected on a StepOnePlus™ Thermal Cycler (Thermo Fischer Scientific ...