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Citations for Thermo Fisher :
8151 - 8200 of 10000+ citations for 6 AMINO 4 METHYL INDOLE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 8 days after transfection antibiotic selection was performed using 6 µg mL-1 blasticidin S (Thermo Fisher Scientific, A1113903) to identify cells which had undergone productive editing ...
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bioRxiv - Cell Biology 2023Quote: ... supplied with the primers for ICP27 and β-actin on a Quant Studio 6 Flex qPCR system (Applied Biosystems).
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bioRxiv - Cell Biology 2022Quote: ... and an oxygen-scavenger system (60□mg ml−1 glucose oxidase, 6□mg□ml−1 β-ME (Gibco, 21985023)) were used for all STORM imaging experiments.
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SHARPIN enhances ferroptosis in synovial sarcoma cells via NF-κB- and PRMT5-mediated PGC1α reductionbioRxiv - Cancer Biology 2023Quote: Yamato cell lines were seeded into 6-well plates and incubated with 5 µM BODIPY 581/591 C11 (Invitrogen) for 30 minutes at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... and SEC using a Superose 6 10/300 GL Increase column (Cytiva, MA) equilibrated in PBS buffer (Thermo Scientific 137mM NaCl ...
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bioRxiv - Neuroscience 2023Quote: ... iPSCs were seeded at a concentration of 150,000 cells per well of a Matrigel-coated 6-well plate in E8 medium (ThermoFisher) containing Rock-inhibitor (10 µM ...
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bioRxiv - Cell Biology 2023Quote: ... and plaque-derived sEVs (n=6) were mixed with NuPAGE lithium dodecyl sulfate sample buffer (NP0007, Thermo Fisher Scientific) at a 4:1 ratio and NuPAGE sample reducing agent (NP0009 ...
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bioRxiv - Genetics 2023Quote: 3 independent total RNA extractions from 30 ovaries from 3-6-day-old RevI-H2i2 flies using Trizol (Invitrogen) were performed ...
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bioRxiv - Pathology 2023Quote: ... Cells were spun down at 350g for 6 minutes and were re-suspended with 4mL of 0.05% Trypsin (ThermoFisher) and incubated at 37°C for 10 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... The RT-qPCR was performed using the Promega® 2x Master Mix and the QuantStudio™ 6 (Applied Biosystems) for 40 cycles ...
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bioRxiv - Neuroscience 2023Quote: ... The parental hiPS cell line (8858-3) was maintained in 6-well plates using StemFlex medium (Life Technologies, A3349401). Cas9 ...
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bioRxiv - Immunology 2023Quote: ... The paraffin blocks were sliced into 5 to 6 μm sections using a Microm HM 325 microtome (Thermo Scientific), and the sections were stained with Hematoxylin and Eosin (H&E) ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA from mating wild-type and EMA2 KO cells were isolated at 6 hpm using TRIzol reagent (Invitrogen). The RNA was treated with Turbo DNase (Ambion ...
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bioRxiv - Cancer Biology 2023Quote: ... was transduced by lentivirus and successfully transduced cells were selected by 6 μg/ml blasticidin (R21001; Thermo Fisher Scientific) for 1-2 weeks ...
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bioRxiv - Cell Biology 2023Quote: Interleukin-6 levels in supernatants were determined using commercially available human IL-6 ELISA kits according to the manufacturer’s protocol (Invitrogen). A detailed description of the procedure are reported in Supporting methods ...
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bioRxiv - Molecular Biology 2023Quote: ... pH 6) at 40°C with vigorous shaking at 1400rpm for 30 minutes using a thermal mixer (Thermo Scientific). The eluted DNA and previous input samples were then amplified using the PCR Primer Mix 3 for Illumina and the VAHTS HiFi Amplification Mix 2 (ND607 ...
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bioRxiv - Genetics 2024Quote: Total RNA was extracted from samples containing 6 OD600 units of mid-log phase cells using Trizol (Invitrogen #15596026) and chloroform ...
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bioRxiv - Cell Biology 2024Quote: ... Quantitative PCR (qPCR) was conducted on a QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems, CA, USA). Relative mRNA levels were determined using the Comparative Ct (△△CT ...
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bioRxiv - Cell Biology 2024Quote: ... coverslips with the gel attached were transferred to a well of a 6-well plate with 1 ml of digestion solution (0.5% Triton X100, 1 mM EDTA pH 8 (AM9260G, Ambion), 50 mM Tris pH 8 (AM9855G ...
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bioRxiv - Cell Biology 2024Quote: ... An overnight incubation with specific antibodies (P16, IL-6 or phospho-H2AX (S139) and cTNT (Invitrogen, #MA-5-12960)) was followed by 2 hours of incubation with secondary antibody conjugated with Alexa Fluor 488 ...
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bioRxiv - Genomics 2024Quote: ... and the relative expression was determined by the 2-ΔΔCt method on a QuantStudio 6 qPCR machine (Thermo Fisher). The levels of mRNAs were normalized to human ACTB.
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bioRxiv - Neuroscience 2024Quote: Brains were dissected from 2-day old adults in PBS and incubated in 1.6 × 10-6 M acridine orange (ThermoFisher) for 5 min at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... equal amounts of RNA were prepared in equal volumes (6 µL) using UltraPure DNase/RNase-free distilled water (Invitrogen). RNA samples were denatured at 95 °C for 3 min and placed on ice immediately to prevent the formation of secondary structures ...
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bioRxiv - Biophysics 2024Quote: ... USA) dissolved in ethanol or 1μL 2.5mg/mL N,N-Dimethyl-6-dodecanoyl-2-naphthylamine (LAURDAN; Thermo Fisher, USA) dissolved in dimethyl sulfoxide to label the EVs ...
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bioRxiv - Cancer Biology 2024Quote: ... typically 1.5 μg of plasmid was reverse transfected into 300,000 293T cells in a well of a 6-well plate using OptiMEM (Gibco) and FuGENE 6 (Promega) ...
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bioRxiv - Biophysics 2024Quote: ... at a 6:1:2 ratio for each virus using the Bac-to-Bac baculovirus expression system (Thermo Fisher). Cell cultures were grown in ESF 921 medium (Expression Systems ...
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bioRxiv - Biochemistry 2024Quote: ... followed by a 6× His-tag was cloned into the BamHI and XbaI sites of the pcDNA 3.1 vector (#V79020, Invitrogen) containing the vascular endothelial growth factor receptor 1 (VEGFR ...
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bioRxiv - Microbiology 2024Quote: ... sg.ORF7a) and genomic RNA (NSP3 region; g.NSP3) (90) on a QuantStudio 6 Pro Real-Time PCR System (Applied Biosystems). Relative mRNA expression was normalized to the levels of GAPDH and expressed relative to the values for control cells using the ΔΔCt method.
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bioRxiv - Molecular Biology 2024Quote: WT and TRBP−/− HeLa cells were plated at 4 x 106 cells / dish into a 9-cm dish and transfected with 6 µg/dish poly(I:C) using Lipofectamine 2000 (Invitrogen). At 0 ...
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bioRxiv - Cell Biology 2024Quote: ... a 6-well was coated with 1 mL of a 1:200 solution of either Geltrex (Gibco™ A1413202) or Cultrex (Bio-Techne 3445-005-01 ...
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bioRxiv - Cell Biology 2024Quote: ... The reaction was incubated for 15 minutes at room temperature and then separated on a 6% DNA retardation gel in 0.5X TBE running buffer (ThermoFisher). The gel was imaged with a SapphireTM multimode imager in the Keck Biophysics facility at Northwestern University.
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bioRxiv - Physiology 2024Quote: ... Cells were grown overnight in glass slides and loaded with 10-6 mol.L-1 4,5-diaminofluorescein-diacetate (DAF-2DA; Molecular Probes) for 30 minutes ...
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bioRxiv - Pathology 2024Quote: ... IRB number: VGHUST105-G7-6-1) were cultivated in Dulbecco’s modified Eagle’s medium (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% heat-inactivated fetal bovine serum ...
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bioRxiv - Plant Biology 2020Quote: ... RNA (4 μg) was treated with TURBO DNA-free DNase (ThermoFisher Scientific) twice ...
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bioRxiv - Biophysics 2021Quote: ... Plunge-freezing was performed using a VitroBot Mark 4 (Thermo Fisher Scientific) with Teflon sheets on both sides and blotting from the back ...
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bioRxiv - Biophysics 2020Quote: ... SDS-PAGE was run using NuPAGE 4-12% Bis-Tris gels (Invitrogen) and stained using a Coomassie stain (SimplyBlue SafeStain ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were resolved on 4-12% Bis-Tris Protein Gels (Thermo Scientific) prior to transfer onto nitrocellulose membranes (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: ... and 4-12% Bolt Bis-Tris Plus pre-cast gradient gels (Invitrogen). 50ug of protein per lane was run and transferred using the Mini Blot module at 20V for 60 minutes onto 0.4µm polyvinylidene fluoride (PVDF ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were stained with 4’,6diamidino-2-phenylindole (DAPI, 1:300, Invitrogen) at 37 °C for 5 min in PBTX solution ...
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bioRxiv - Cancer Biology 2021Quote: Live organoids were stained using Calcein AM (4 mM stock from Invitrogen). The staining dye solution was prepared by adding 1.0 µL of Calcein AM in 8 mL staining buffer (MBD-STA50 ...
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bioRxiv - Cell Biology 2019Quote: ... on a precast NuPAGE™ 4-12 % Bis-Tris Protein Gels (Invitrogen). Sample loading was adjusted based on equivalent OD600 in each lane ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were separated on a NuPage 4-12% Bis-Tris Gel (Invitrogen) and transferred onto a Hybond P 0.45 polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2020Quote: HepG2 cells were seeded into 4-well glass-bottomed chambers (Nunc,IL) at a density of 1×105/well in DMEM medium and cultured for 24 h ...
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bioRxiv - Cell Biology 2020Quote: ... and equal amounts were resolved on NuPAGE 4–12% precast gels (Invitrogen). Proteins were transferred from gels to PVDF membrane (Immobilon-FL ...
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bioRxiv - Cell Biology 2020Quote: ... and run on a NuPAGE 4-12% Bis-Tris gel (Life Technologies). The gels were transferred onto a PVDF membrane by wet transfer ...
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Microbial signals and lymphotoxin drive TNF-independent death of A20 and ABIN-1 deficient epitheliumbioRxiv - Immunology 2021Quote: ... followed by resolution on NuPage precast 4-12% Bis-Tris gels (Invitrogen) and transferred to PVDF for immunoblotting.
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bioRxiv - Genetics 2021Quote: ... and 4 × NuPAGE LDS (lithium dodecyl sulfate) Sample Buffer (Invitrogen by ThermoFisher). Samples were denatured by heating to 80°C for 15 minutes ...
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bioRxiv - Genetics 2021Quote: ... and 4 × NuPAGE LDS (lithium dodecyl sulfate) Sample Buffer (Invitrogen by ThermoFisher). Samples were denatured by heating to 80°C for 15 minutes ...
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bioRxiv - Genetics 2021Quote: ... and hold at 4°C in a Veriti Thermal Cycler (Applied Biosystems). Agarose gel electrophoresis (1.5% Top Vision Low Melting Point Agarose gel (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2021Quote: ... containing a 4:1:1 mixture of Lipofectin transfection reagent (ThermoFisher Scientific), water and BAC DNA (~ 15 μg DNA per larva) ...