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Citations for Thermo Fisher :
8001 - 8050 of 10000+ citations for 6 AMINO 4 METHYL INDOLE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: Primary cultures of hippocampal neurons were established by isolating E16–17 C57BL/6 embryonic hippocampal tissues in HBSS (Gibco) and dissociating tissues in 0.25% trypsin (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2022Quote: ... with a total reaction volume of 12.5 μL using a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems). Gene specific primers were used to amplify GDNF (forward sequence 5-GCAGACCCATCGCCTTTGAT-3 and reverse 5-CCACACCTTTTAGCGGAATGC-3).
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bioRxiv - Bioengineering 2022Quote: ... Cells were then treated with 6 mM CHIR99021 (STEMCELL; 72052) in DeSR1: DMEM/Ham’s F12 (Thermo Fisher Scientific; 11039021), 1X MEM-NEAA (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... fused with an N-terminal 6×His-tag and cloned into expression vector pPIC9 (Thermo Fisher Scientific, California, USA). Correctness of the resulting constructs was confirmed by DNA sequencing prior to introduction into Pichia pastoris strain GS115 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... A549 cells were embedded in 6-well culture plates at a density of 20.000 cells/well in 0.8% low melting point agarose (Thermo Scientific) on the top of a layer of low melting point agarose at 1.6% ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were plated at 1 x 10^6 cells per well of a 6 well plate or 5 x 10^6 cells per T25 flask in stage 2 media consisting of 47.5% IMDM (Gibco), 47.5% F12 (Gibco) ...
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bioRxiv - Microbiology 2021Quote: ... coli cells or 6 10e6 Erwinia oleae cells pre-grown for 3.5 h in DMEM with 25 mM HEPES (Invitrogen) were mixed with EDTA (1 mM ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were plated in 6 well plates and transfected with 5 μl Lipofectamine RNAiMAX transfection reagent and siRNAs (Invitrogen) at 50 nM final concentration according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... and axial lymph nodes of naïve un-manipulated WT C57BL/6 mice were isolated and processed in RPMI 1640 (Gibco) as above ...
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bioRxiv - Neuroscience 2020Quote: ... undifferentiated mESCs were plated at a density of 9 x 104 cells/cm2 in wells of a 0.1% gelatin-coated 6-well dish (day 0) in N2B27 serum-free medium: DMEM/F12 (Invitrogen), Neurobasal media (Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: Wild type OL61 glioma cells (5.0 x 105) were cultured in a 6-well plate in 1mL of media (DMEM, Thermofisher Cat# 12430054 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were plated in a 6 cm dish and 24 hours later plasmids were transfected with Lipofectamine 2000 (Invitrogen) based on manufacture’s protocol and then incubated for 24 hours before immunoprecipitation experiments.
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bioRxiv - Molecular Biology 2020Quote: ... flushed with argon prior to adding hydrochloric acid (3 mL of 6 M sequencing grade solution; Thermo Scientific #PI24308). Sealed tubes were kept at 125° C for 48h (oil bath ...
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bioRxiv - Microbiology 2021Quote: Sub-confluent cells on coverslips in 6 well plates were transfected with 2μg of DNA using the Lipofectamine 3000 transfection system (Invitrogen). Eighteen to twenty-four hours post transfection cells were fixed with 4% PFA for 15 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... MDCK cells were grown to 90-95% confluence in 6-well tissue culture plates in DMEM (ThermoFisher Scientific/Gibco) containing 10% FBS (ThermoFisher Scientific/Gibco ...
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bioRxiv - Bioengineering 2021Quote: ... was dissolved under stirring in 15 mL of an alginate solution (10 mg/mL-1) formed in 0.1 M MES buffer at pH 6 (ThermoFisher). Sulfo-NHS (41 mg ...
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bioRxiv - Developmental Biology 2021Quote: ... A range of 1,000–11,000 cells per well was seeded into wells prefilled with 150 μL Essential 6™ (E6 medium) (Gibco, Carlsbad ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA/protein complexes were then electrophoreticed by 6% native polyacrylamide gel and transferred to nylon membrane (Thermo Fisher Scientific). RNA was cross-linked with a UV lamp at a distance of 0.5 cm from the membrane for 2 min ...
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bioRxiv - Molecular Biology 2022Quote: ... 1.5X105 cells were reverse transfected with 120 pmol of siRNAs in 6-well plates using Lipofectamine RNAiMAX (ThermoFisher Scientific). Cells were harvested for RNA extraction 72 hours after transfection ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were seeded into 6-well plates and transfection protocol was followed according to manufacturer’s instructions (Cat.#13778075, Invitrogen).
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bioRxiv - Microbiology 2022Quote: ... or in 2 ml liquid MSgg with 7-d-old tomato seedlings in a 6-well plate (Thermo Scientific). Each well contained one seedling ...
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bioRxiv - Pathology 2022Quote: ... resulting from UA were determined via a membrane-permeant JC-1 dye (5, 50, 6, 60 -tetrachloro-1, 10,3,30 -tetraethylbenzimidazol-carbocyanine iodide, Molecular Probes). JC-1 dye accumulates in mitochondria in a potential-dependent manner ...
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bioRxiv - Cancer Biology 2022Quote: T98G cells (2 × 105 into 6 well plate) were reverse transfected with siRNAs (Table S3) using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... The size and integrity of the biotinylated RNA were checked on a 6% Urea gel (Thermo Scientific, Darmstadt, Germany). Immediately before use ...
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bioRxiv - Pathology 2022Quote: Pathogen-free MPT cells (BU-MPT immortalized cells) were plated in 6-well plates in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco) supplied with 10% FBS (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and mrc2 was measured by qPCR (relative to 18s) on QuantStudio 6 Flex Real-time PCR system (Applied Biosystems).
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bioRxiv - Plant Biology 2019Quote: ... The RT-qPCR was conducted in triplicates on a QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems) with SYBR Green Master Mix (Vazyme Biotech) ...
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bioRxiv - Genetics 2019Quote: ... The chromatin with the antibodies was incubated with 6 μl of Dynabeads Protein G (Thermo Fisher Scientific, Cat# 10003D) for one hour ...
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bioRxiv - Systems Biology 2019Quote: ... The membrane inserts were placed into 6-well plates containing 1.5 ml maintenance medium consisted of F12/DMEM (Gibco) supplemented with N-2 Supplement (Gibco ...
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bioRxiv - Immunology 2019Quote: Serum IL-6 and IL-17A concentrations were assessed by ELISA according to the manufacturer’s instructions (Thermo Fisher Scientific).
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bioRxiv - Genomics 2019Quote: ... HEK293T cells were seeded at a density of 0.5×106 per 6-well in low antibiotic growth media (DMEM with 10% FBS (Gibco), 0.1% penicillin/streptomycin ...
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bioRxiv - Biochemistry 2019Quote: ... were prepared by growing for 6–12 h on CA then suspended in Dulbecco’s phosphate buffered saline (DPBS, Gibco) to an absorbance of 1.0 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Reactions were incubated for 60 min at room temperature and run on a 6% DNA Retardation gel (Invitrogen™) in TBE buffer ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: Liver-Chips were stained in the upper channel with 5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate (CDFDA) (Thermo Fisher) to visualize bile canaliculi and MRP2 activity ...
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bioRxiv - Neuroscience 2019Quote: ... harbouring the full-length cDNA coding for the human muscle 6-phosphofructo-1-kinase muscle isoform (PFK1-M)3 (accession number, NM_000289.1) using Lipofectamine LTX-PLUS Reagent (Life Technologies) according with manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then plated at 1.6 × 106 cells per 6-well and allowed to differentiate for 5-7 days in RPMI-1640 medium (GIBCO), 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Synthetic Biology 2020Quote: ... human embryonic kidney 293 (HEK 293)(ATCC) cells were seeded in a 6-well plate at 0.3×106 cells/well in DMEM (ThermoFisher) supplemented with 10% FBS and 1% penicillin-streptomycin (Gibco ...
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bioRxiv - Immunology 2020Quote: ... whereby the transfected population is selected for removal of HXGPRT and replacement with the complementation allele in 6-thioxanthine selection medium [177 µg/mL of 6-thioxanthine (TRC, cat# T385800) in 4.5 g/liter D-glucose in GlutaMAX DMEM (Gibco), with 1% dialyzed FBS (Omega Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... Transfection of HCT116 cells (0.6×106 cells/well in 6-well plate) with siRNA oligonucleotides was performed using Lipofectamine RNAiMAX (Invitrogen).
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bioRxiv - Microbiology 2020Quote: ... 6-well plate format) mock- or virus-infected (MOI of 0.01) were extracted using TRIzol reagent (Thermo Fisher Scientific). Superscript® II Reverse Transcriptase (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons were plated at a density of 6 ×105 cells/cm2 and cultured in Neurobasal media (Invitrogen, Carlsbad, CA) supplemented with B-27 (Invitrogen) ...
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bioRxiv - Physiology 2019Quote: ... Each 6 μL assay master mix (AMM) consisted of 3 μL of TaqMan® Gene Expression assay 20X (ThermoFisher) and 3 μL of 2X Assay Loading Reagent (Fluidigm) ...
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bioRxiv - Zoology 2021Quote: ... and the cDNA was used for qPCR analysis using the QuantStudioTM 6 Flex Real-Time PCR System (Applied Biosystems) and SYBR Green detection system (TOYOBO ...
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bioRxiv - Immunology 2020Quote: ... male C57BL/6 recipient mice were first primed intraperitoneally (i.p.) with 50 mg ovalbumin (OVA) in alum (Thermo Scientific) at 2:1 v:v ratio in 100 μL total volume ...
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bioRxiv - Bioengineering 2020Quote: We used a mixture of 6 μm and 2 μm diameter fluorescence beads (C16509, Thermo Fisher; F8825 Thermo Fisher) in our experiment ...
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bioRxiv - Bioengineering 2020Quote: We used a mixture of 6 μm and 2 μm diameter fluorescence beads (C16509, Thermo Fisher; F8825 Thermo Fisher) in our experiment ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were incubated with transfection complexes for 5-6 hours and then replated onto #1.5 coverslips (Warner Instruments) coated with Collagen IV (Gibco). Cells were imaged in DMEM/10% FBS ...
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bioRxiv - Genetics 2021Quote: ... Cells were plated at 0.3-0.4 × 106 in 1.5 ml media in 6-well plates-24 hours before transfection with either rCro or lipofectamine 3000 (ThermoFisher) or Lipofectin (99-102 ...
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bioRxiv - Immunology 2020Quote: ... animals previously injected with vaccine or PBS where injected 24 hrs before sacrifice in the same footpad with 30 μL of 0.5 mM 5-and 6-carboxyfluorescein diacetate succinimidyl ester (CFSE) (Invitrogen). For assessing migration after 24 hrs ...
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bioRxiv - Microbiology 2019Quote: Bacterially expressed ZIKV EDIII proteins (C-terminal 6 × His-tag) were conjugated to Ni-NTA Magnetic beads (Thermo Scientific) following manufacturers protocol ...