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Citations for Thermo Fisher :
8001 - 8050 of 10000+ citations for Hydrogen Peroxide Fluorescent Detection Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... 2% B27 (Fisher Scientific, 17504044), 0.1% P/S ...
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Tumor microenvironment acidosis favors pancreatic cancer stem cell properties and in vivo metastasisbioRxiv - Cancer Biology 2024Quote: ... B27 (2%, Thermo Scientific, #12587010). Cells were dissociated to single cells by extensive pipetting or by using a 25 G needle ...
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bioRxiv - Genomics 2023Quote: ... medium was removed and cells were lysed directly on plate in RNA lysis buffer and processed using the MagMAX® mirVana Total RNA Isolation Kit (Thermo Fisher Scientific Inc., Cat. No. A27828) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... A total of 2 μg per sample was reverse transcribed using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... ATPase was assayed by a continuous spectrophotometric method using a 2-amino-6-mercapto-7-methylpurine ribonucleoside/purine nucleoside phosphorylase reaction to detect the released inorganic phosphate (EnzChek Kit; Life Technologies, California, USA) (53) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... For cloning of the NvCoREST cDNA (Supplementary Fig. 2) the fragments were cloned using the CloneJET PCR Cloning Kit (Thermo Fisher Scientific, K1231). To generate the NvPOU4::NvCoREST-mCherry construct the backbone was amplified from the NvPOU4: ...
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bioRxiv - Developmental Biology 2021Quote: A dam at E16.5 in gestation was given an intraperitoneal (IP) injection with EdU (5-ethynyl-2’-deoxyuridine) from the Click-iTTM EdU Alexa FluorTM 647 Imaging Kit (Invitrogen, Carlsbad, CA, USA) at a dosage of 0.25mg /10g body weight ...
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bioRxiv - Molecular Biology 2021Quote: ... the nascently synthesized DNA was labeled with 5-ethynyl-2′-deoxyuridine (EdU) and Alexa Fluor 488 azide through Click reaction using a Click-iT EdU Imaging kit (Life Technologies, cat# C10337) and then cells were stained with propidium iodide (PI ...
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Characterization of GRK5 as a novel regulator of rhabdomyosarcoma tumor cell growth and self-renewalbioRxiv - Cancer Biology 2019Quote: ... Cell cycle analysis was done with flow cytometry on cells pulsed with EdU for 2 hours using the Click-iT EdU Alexa Fluor 647 Flow Cytometry Assay kit (Life Technologies, Carlsbad, CA).
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bioRxiv - Genetics 2021Quote: ... Poly (A) RNA was obtained from 2 μg of total RNA using the Dynabeads Oligo (dT)25-61005 Kit (Thermo Fisher, CA, USA), with two rounds of purification ...
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bioRxiv - Cell Biology 2021Quote: Neonatal mouse ventricular myocytes (NMVMs) were isolated from 1-2 day old mice following Pierce™ primary isolation kit (88281, Thermo Fisher Scientific). After 72 hours in standard culture conditions ...
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bioRxiv - Cancer Biology 2020Quote: ... The most frequently used ES-2 cell line was authenticated by Eurofins Genomics using AmpFlSTR® Identifiler® Plus PCR Amplification Kit (Thermo Fisher) for STR profiling ...
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Role of autophagy in sepsis-induced skeletal muscle dysfunction, whole-body metabolism, and survivalbioRxiv - Cell Biology 2021Quote: The concentration of IL-1β in GAS muscle extracts at 2-day post-surgery was measured using Mouse IL-1 β ELISA Kit (Invitrogen #88-7013), according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: Cumulative curve of the thymidine analog 5-Ethynyl-2’-deoxyUridine (EdU) incorporation is performed using Click-iT™ Plus EdU Alexa Fluor™ 647 Imaging Kit (ThermoFisher; C10640). Briefly ...
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bioRxiv - Cell Biology 2022Quote: ... Preparation of the samples for paired-end sequencing on an Illumina NextSeq500 (2×35 bp) was done with Collibri™ ES DNA Library Prep Kit for Illumina Systems (Thermo Fisher, A38605024).
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bioRxiv - Microbiology 2022Quote: ... 5 μg of each RNA sample was incubated twice with 2 units of TURBO DNase (TURBO DNA-free Kit, Ambion, Austin, TX, USA) for 30 min at 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids were targeted in a second bioreactor sampling experiment (n=2 for both bioreactors) for which the Plasmid Miniprep kit was used according to manufacturer’s instruction (Thermo Fisher Scientific, Waltham, US), with the addition of a step homogenizing the cells before processing ...
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bioRxiv - Neuroscience 2020Quote: Proliferation of pericytes was determined by incorporation of the thymine analogue 5-ethynyl-2’-deoxyuridine (EdU) with the Click-iT®Assay Kit (Life Technologies C10340) according to manufacturer’s instructions ...
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bioRxiv - Zoology 2020Quote: Libraries for whole genome sequencing were generated using the Ion AmpliSeq Kit for Chef DL8 and Ion AmpliSeq SARS-CoV-2 Research Panel (Thermo Scientific, Waltham, MA). Libraries were sequenced using an Ion 520 chip on the Ion S5 system using the Ion 510™ & Ion 520™ & Ion 530™ Kit ...
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bioRxiv - Systems Biology 2020Quote: ... The amount of VEGF contained in the thawed medium was measured using the protocols and reagents from the VEGF Human ELISA Kit (BMS277-2, Thermo Fisher Scientific, USA), and the amounts of PEDF were measured using a Human ELISA Kit (RD191114200R ...
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bioRxiv - Microbiology 2021Quote: Libraries for the whole genome sequencing were generated using the Ion AmpliSeq Kit for Chef DL8 and Ion AmpliSeq SARS-CoV-2 Research Panel (Thermo Scientific, Waltham, MA). Libraries were sequenced using an Ion 520 chip on the Ion S5 system using the Ion 510™ & Ion 520™ & Ion 530™ Kit ...
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bioRxiv - Developmental Biology 2019Quote: ... samples were incubated for 8 minutes in TSA Plus Fluorescein solution (1/50 TSA Plus Fluorescein in 1x Amplification Buffer (Tyramide Signal Amplification Labeling Kit No. 2; Molecular Probes, Thermo Fisher Scientific)) in darkness ...
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bioRxiv - Microbiology 2021Quote: ... Four microliters RNA was used for real-time RT-qPCR to detect and quantify N gene of SARS-CoV-2 using TaqMan™ RNA-to-CT 1-Step Kit (Thermo Fisher Scientific) as described [41] using the following primers and probes ...
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bioRxiv - Biophysics 2020Quote: ... cells were incubated with 2 μg/μl EdU (Click-i™ Plus EdU Alexa Fluor™ 488 Flow Cytometry Assay Kit, Invitrogen, USA) for 1 h ...
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bioRxiv - Immunology 2020Quote: ... A viability dye was included in all Duraclone panels (Live/dead fixable dead cell stain kit from ThermoFisher Scientific or DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate from ThermoFisher Scientific (Courtabœuf, France)) ...
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bioRxiv - Cell Biology 2021Quote: ... antibody was used for immunoprecipitation of BRCA1 complexes from 2 mg of nuclear extract using Pierce Classic IP Kit (Thermo Fisher Scientific, 26146) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... Sections from the AstV-ND-1 patient’s brain and a control human brain were mounted onto microscope slides and hybridized with either astrovirus or actin probes using a QuantiGene (R superscript circularized) ViewRNA ISH Tissue 2-Plex Assay kit (Affymetrix Cat No. QVT0012).
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bioRxiv - Molecular Biology 2024Quote: T7 double stranded DNAs (T7-dsDNAs) were amplified by T7-tagged primer pairs (Table 2) by Phire Hot Start II PCR Master Mix kit (Thermo Fisher, Cat # F125L) using proper plasmids as templates ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA (2 μg) was reverse transcribed in a final volume of 10 μL using SuperScript™ III Reverse Transcriptase kit (ThermoFisher Scientific®,) with oligo-p(dt)15 (Roche® ...
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bioRxiv - Synthetic Biology 2023Quote: ... The second-round PCR products were size-selected using 2% agarose gel and purified using GeneJET Gel Extraction Kit (Thermo Fisher Scientific #K0691). The samples were pooled ...
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bioRxiv - Microbiology 2023Quote: ... Four microliters RNA was used for real-time RT-qPCR to detect and quantify N gene of SARS- CoV-2 using TaqMan™ RNA-to-CT 1-Step Kit (Thermo Fisher Scientific) as described (73 ...
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bioRxiv - Cell Biology 2023Quote: ... Click-iT reaction buffer additive 1 and Click-iT reaction buffer additive 2 from the Click-iT™ Protein Reaction Buffer Kit (Thermo Fisher Scientific) were added to the sample according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... the first-strand cDNA was synthesized from 2 µg of total RNA with the Maxima First-Strand cDNA synthesis kit (ThermoFisher Scientific, Darmstadt, Germany). A StepOnePlus Real-Time PCR system (Applied Biosystems ...
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bioRxiv - Microbiology 2023Quote: ... malayi cell suspensions were assessed for proliferative activity using the thymidine analog EdU (5-ethynyl-2’-deoxyuridine) and the Click-iTTM EdU Cell Proliferation Kit for Imaging with the Alexa FluorTM 488 dye (Thermo Fisher, Waltham, MA). Cells dissociated from B ...
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bioRxiv - Developmental Biology 2023Quote: Cell cycle changes were additionally assessed using the 5-ethynyl-2’-deoxyuridine (EdU) assay using the Click-iT™ EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit (ThermoFisher, C10418). HUH7.5 cells were incubated at 37 °C for 2 hours with 10 µM EdU using the Click-iT EdU Assay Kit (Thermofisher ...
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bioRxiv - Plant Biology 2023Quote: ... cDNA was synthesized with 2 µg total RNA and random hexamer primers using High Capacity cDNA Reverse Transcription kit (Applied Biosystems, United States) as per manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Peripheral blood mononuclear cells (PBMCs) from donor # 1 and donor #2 were isolated and reprogrammed into iPSCs using the Sendai Reprogramming Kit (Thermo Fisher, cat # A34546). CFLine#1 was generated from donor #1 while CFLine#2a and CFLine#2b were derived from donor # 2 ...
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bioRxiv - Molecular Biology 2023Quote: ... and the eluted chromatin and input were treated for 2 hours at 55℃ with proteinase K (supplied with PureLink Genomic DNA Mini Kit, ThermoFisher, Cat# K1820-02). The input and eluted chromatin were subsequently de-crosslinked overnight at 65℃ ...
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Epigenetic deregulation of IFN and WNT pathways in AT2 cells impairs alveolar regeneration (in COPD)bioRxiv - Cell Biology 2023Quote: RNA was isolated from flash frozen pellets of 2×104 sorted AT2 cells using the Arcturus Picopure RNA Isolation kit (Thermo Fisher Scientific, KIT0204) following manufacturer’s instructions ...
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Higher meiotic chromosome condensation: a potential function of kinetochore through polo-like kinasebioRxiv - Cell Biology 2024Quote: Total RNA was extracted from meiotic cell culture of 1.5-2 OD600 (∼2.5 x107 cells) using TRIzol® reagent and PureLink® RNA mini kit (Ambion Life Technologies™). The cell pellet was re-suspended in TRIzol® and cell lysis was done using DEPC treated sterile 0.5 mm glass beads ...
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bioRxiv - Microbiology 2024Quote: ... Four microliters RNA was used for real-time RT-qPCR to detect and quantify genomic RNA of SARS-CoV-2 N gene using TaqMan™ RNA-to-CT 1-Step Kit (Thermo Fisher Scientific) as described2 using the following primers and probes ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... media was removed and cells were incubated with standard bath solution (SBS) containing 2 μM Fura-2 acetoxymethyl ester (Fura-2 AM; Thermo Fisher Scientific, Paisley, UK) and 0.01% pluronic acid for 1 h at 37 °C ...
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Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunitbioRxiv - Biochemistry 2022Quote: ... 500 mM KCl, 7.5% w/v RNAse free sucrose, 2 mM MgCl2, 75 mM NH4Cl, 2 mM puromycin [Fisher Scientific], and 2 mM DTT) by gentle pipetting ...
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bioRxiv - Evolutionary Biology 2021Quote: ... optical densities were determined in 200 µl culture in microtiter plates by spectrophotometry in a plate reader (Spectramax M5, Applied Biosystems, United States) and 20 µl of culture were transferred into 4 ml of fresh MMAB-medium ...
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bioRxiv - Molecular Biology 2020Quote: ... We then diluted these saturated cultures 1:20 in sterile water and then inoculated 3 µL into 96-well humidified plates (Nunc Edge Plates (Thermo Scientific, Waltham, MA)) with 150 µL of YPD or SD-CSM per well ...
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bioRxiv - Cancer Biology 2020Quote: ... The specimen itself was floated on the water surface inside a standard multi well plate (Thermo Scientific Nunclon Delta Surface 96 well plates). Those are needed to be tape sealed in order to prevent water creep under ultrasound irradiation ...
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bioRxiv - Biochemistry 2022Quote: ... OD600 and fluorescence were measured on a Biotek Synergy H1 MF plate reader using clear-bottom 96-well plates (Thermo Scientific Nunc, #265301). Prior to plate reader measurements ...
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bioRxiv - Neuroscience 2020Quote: ... or NAD standard were combined with 50 μl of reagent in a 96-well plate (Nunc™ F96 MicroWell™ White Polystyrene plate, ThermoFisher Scientific). Luminescence was recorded at approx ...
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bioRxiv - Neuroscience 2020Quote: ... ECL was added to the plate and luminescence was measured with 1 s integration time using Varioskan Flash plate reader (Thermo Fisher Scientific, USA). Levels of TRKB:PTPσ interaction were evaluated by ELISA using a similar protocol ...
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bioRxiv - Synthetic Biology 2019Quote: ... after which the lysates with the beads were transferred to a 96-well filter plate (Thermo Scientific, USA, Nunc 96-well filter plates), placed over a 96-well collection plate ...