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Citations for Thermo Fisher :
8251 - 8300 of 10000+ citations for Hydrogen Peroxide Fluorescent Detection Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... immunoblotted with specific primary and secondary antibodies and detected by chemiluminescence with the ECL detection reagents from Thermo Fisher or Millipore ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA was extracted from SARS-CoV-2-infected Vero-E6 cells and purified with an Oligo (dT) kit (Thermo Scientific, Wilmington, DE, USA). The polyA purified RNA was fragmented and subjected to IP using an m6A-specific antibody ...
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bioRxiv - Genomics 2022Quote: ... Template amplification and enrichment as part of the manual workflow for the Ion S5 systems were performed with the Ion OneTouch 2 system using an Ion 540 Kit (Life Technologies, Carlsbad, California, USA). The thirty-two samples were multiplexed on an Ion 540 chip and sequenced using an Ion GeneStudio S5 Semiconductor Sequencer (Life Technologies Holdings Pte Ltd. ...
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Can Intestinal Absorption of Dietary Protein Be Improved Through Early Exposure to Plant-Based Diet?bioRxiv - Zoology 2020Quote: ... 2 µg of RNA from each sample was reverse transcribed using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA) to obtain a 20 µl cDNA solution ...
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bioRxiv - Plant Biology 2020Quote: ... Genomic DNA was isolated from a leaf disc (2 mm diameter) taken from individual barley seedlings using the Phire Plant Direct PCR Kit (Thermo Fisher Scientific, Waltham, USA). An aliquot of 0.5 μL crude plant extract was used as a template for cas9 and InDel PCR detection in a reaction containing ...
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bioRxiv - Cancer Biology 2021Quote: Cell proliferation was examined by 5-bromo-2-deoxyuridine (BrdU) cell proliferation enzyme-linked immunosorbent assay (ELISA) according to the kit manufacturer’s protocol (Life Technologies, Grand Island, NY, USA). The cells were incubated in presence of various concentrations of CLEFMA (0 – 10 µM) ...
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bioRxiv - Biochemistry 2020Quote: ... coli PepP coding sequence was PCR-amplified (primers 1 and 2) from BW25113 genomic DNA prepared using a GeneJET Genomic DNA Purification Kit (Thermo Scientific, Cat No. K0721) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... from 3 individual (1 young and 2 old) mice and mRNA was extracted with the Dynabeads™ mRNA DIRECT™ Purification Kit (61011 Thermo Fisher Scientific). Reverse transcription was performed with the Maxima H Minus Reverse Transkriptase (EP 0751 Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: Cardiomyocytes were isolated from the left ventricle of 1–2-day-old Sprague-Dawley rats following the protocol from an isolation kit (Cellutron Life Technologies, cat#nc-6031). Myocytes were then plated on cover slips coated with laminin (Life tech #23017-015 ...
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bioRxiv - Immunology 2022Quote: ... Murine IFNγ levels and IL-2 levels were measured using the Mouse Interferon gamma (IFNγ) Uncoated ELISA Kit (Thermo Fisher Scientific, 88-7314-76) or IL-2 Mouse Uncoated ELISA Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... The purified sample was recovered spinning for 2 minutes at 1000 x g and finally quantified using the Pierce™ BCA Protein Assay kit (ThermoFisher Scientific, Monza, Italy) and stored in aliquots at −80°C until use ...
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bioRxiv - Plant Biology 2019Quote: ... A total of 2 ug of RNA was used to synthesize cDNA with SuperScript IV VILO Master Mix with ezDNase Enzyme kit (Thermo Fisher Scientific, CAT: 11766050) following the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2019Quote: ... samples were incubated for 8 minutes in TSA Plus Fluorescein solution (1/50 TSA Plus Fluorescein in 1x Amplification Buffer (Tyramide Signal Amplification Labeling Kit No. 2; Molecular Probes, Thermo Fisher Scientific)) in darkness ...
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bioRxiv - Cell Biology 2021Quote: ... We isolated neonatal cardiomyocyte (postnataTl 2 – 3 days) from Gja1M213L/M213L and Wildtype (WT) mice using PierceTM Primary Cardiomyocyte Isolation Kit (Thermo Fisher Scientific, Walthman, MA) following manufacturer protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... for 2 min at 37 °C and stained using the LIVE/DEAD™ Fixable Violet Dead Cell Stain Kit (Thermo Fisher Scientific, Waltham, USA) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... The sequencing template was prepared by emulsion PCR in a OneTouch 2 instrument using an Ion PGM OT2 HiQ View kit (ThermoFisher Scientific, Waltham, MA, USA). HTS was performed in an Ion Torrent PGM platform with an Ion 316 Chip Kit v2 BC (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell proliferation was also assessed by 5-ethynyl-2’-deoxyuridine (EdU) incorporation assay using Click-iT™ EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... The concentration of isolated RNA was quantified using a NanoDrop® spectrophotometer and 1-2 µg was used for cDNA synthesis with a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems™, 4368814). qPCR reactions were conducted using a 7500 Real-Time PCR System (Applied Biosystems™ ...
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bioRxiv - Cell Biology 2023Quote: ... each peptide sample (approximately 2 mg) was tapplied to a Pierce High-Select™ TiO2 Phosphopeptide Enrichment Kit (Thermo Fisher Scientific, Cat No: A32993). After preparing spin tips as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... 2 μg of total RNA was reverse transcribed into cDNA using a High-Capacity RNA-to-cDNA kit (#4387406, Applied Biosystems, Waltham, MA, USA) and sequences were amplified using Taq DNA polymerase that had been supplied with a PCR master mix (#4324018 ...
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bioRxiv - Immunology 2023Quote: 5 x 106 MT-2 cells were treated and subsequently underwent lysis using NE-PER™ Nuclear and Cytoplasmic Extraction kit (ThermoFisher cat. no. 78833) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... 40 µL nuclease-free water (to reach 100 µL final volume) and 2 µL DNase I (Thermo Scientific, DNase I, RNase-free kit, #EN0521) followed by 30 min incubation at 37°C ...
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Spray-induced gene silencing (SIGS) as a tool for the management of Pine Pitch Canker forest diseasebioRxiv - Plant Biology 2024Quote: ... Fluorescein-labeled dsRNA was synthesized using 2 µL of the plasmid pT777T-YFP in 20 µL reactions of the MEGAscriptTM RNAi Kit (Thermo Fisher, Waltham, MA, USA) with 2 µL of the Fluorescein labeling mix (Roche ...
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bioRxiv - Microbiology 2024Quote: ... 440-2 and 445-3 were generated using one of two methods 1) using a Mouse IgG1 Fab and F(ab′)2 Preparation Kit (Thermo Scientific™ Pierce™) which uses immobilized Ficin for cleavage 2 ...
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bioRxiv - Biochemistry 2024Quote: ... The cDNA synthesis reaction was then performed using 2 μg of RNA for each sample with the High-Capacity cDNA Reverse Transcription® kit (Thermo Fisher Scientific, USA).
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bioRxiv - Microbiology 2021Quote: Whole ear pinnae homogenates were diluted in 10-fold steps in LB medium and 10 µl of undiluted and diluted samples were pipetted onto sheep blood agar plates (Columbia Agar with sheep blood Plates; Oxoid, Cat. # PB0123A, Thermo Fisher Scientific, Waltham, Massachusetts). After incubation for 18 h at 37 °C ...
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bioRxiv - Microbiology 2020Quote: Ten μl 0.0125% DMSO in PBS was spotted on all 384 wells each of imaging-compatible 384-well plates (Matrix plates #4332, Thermo Fisher Scientific, Waltham, USA) using a Matrix WellMate dispenser and normal bore Matrix WellMate tubing cartridges (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: Chimeric proteins were diluted to 1 μg/ml in carbonate buffer (pH 9.6) and used to coat polystyrene 96-well high-binding ELISA plates (100 μl/well; Nunc-Immuno plate with MaxiSorp surface). After overnight incubation at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... The positive control was diluted in 1.5 mM of sodium carbonate buffer pH 9.6 to 20 µg/ml and used for plate coating by adding 100 µL per well to a Nunc MaxiSorp(tm) flat-bottom plate (Thermo Fisher, Waltham, Massachusetts, USA) and incubating overnight at 4 °C ...
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bioRxiv - Bioengineering 2021Quote: ... 200uL of the cell suspension was plated on YP-SUC plates and spread throughout the plate using glass beads (Fisher Scientific, #11-312B 4mm). The plates were wrapped in sterile ...
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bioRxiv - Evolutionary Biology 2022Quote: ... From the preculture plate we used 5 μl to inoculate each of four different wells of a 384-well plate (Sigma-Aldrich, Nunc®, flat-bottom) each containing 55 μl of SM containing:
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bioRxiv - Synthetic Biology 2022Quote: ... The plate was then incubated at 37° C under continuous shaking (420 rpm) using a Varioskan Lux plate reader (Thermo Fisher Scientific, Waltham, USA). Bacterial growth (OD600nm ...
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bioRxiv - Systems Biology 2023Quote: ... HeLa spheroids were serum-starved for 4h by an initial wash in 200 µl (96-well plate) or 3 ml (24-well plate) DMEM high-glucose (Thermo Fisher Scientific #41966-029) supplemented with another 2 mM of L-Glutamine ...
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bioRxiv - Genomics 2023Quote: ... 200 μL of bead suspension was aliquoted into individual wells of a deep well 96-well plate (Nunc 96-Well DeepWell Plates with Shared-Wall Technology, Thermo Scientific, Cat. No. 260251) and 14 μL of 5.675nM (1:40 from 227nM working stock made fresh ...
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bioRxiv - Zoology 2024Quote: Adult worms from a communal tank were moved to individual wells in the center of a 24-well plate or 12-well plate (Fisher Scientific #07-200-82). We mounted the plate on an elevated platform within an enclosed behavioral rig ...
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bioRxiv - Microbiology 2020Quote: ... Immulon 4 HBX 96-well ELISA plates (Thermo Fisher Scientific) were coated with 2 μg/ml of recombinant trimeric S protein (50ul per well ...
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bioRxiv - Microbiology 2020Quote: ... Immulon 4 HBX 96-well ELISA plates (Thermo Fisher Scientific) were coated with 2 µg/ml of recombinant trimeric S protein (50 µL per well ...
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bioRxiv - Cancer Biology 2021Quote: ... and loaded on Optical 96-Well Reaction Plates (Applied Biosystems) in triplicate ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were dissociated from plates using trypsin (Gibco, 25300-054) and 2×105 cells were collected and centrifuged at 500 RCF for 5 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... flat-bottomed 384-well plate (Thermo Fisher Scientific, Loughborough, UK). The plates were stored at 4°C for 72 hours to allow the procollagen to bind and coat the plates ...
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bioRxiv - Cell Biology 2019Quote: ... Plates were washed once with 1X HBSS (Invitrogen 14065-056) and 75 μL 0.1 % (vol/vol ...
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bioRxiv - Cell Biology 2020Quote: ... scaffolds were placed in 24-well culture plates (Fisher Scientific), supplemented with culture medium ...
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bioRxiv - Immunology 2021Quote: Assays were performed in MaxiSorp 96-well plates (Thermo Fisher) coated with 100 ul of recombinant SARS-CoV-2 S ...
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bioRxiv - Immunology 2021Quote: ... ninety-six well Maxisorp plates (Nalge Nunc, Rochester, NY, USA) were coated with ConSOSL.UFO.664 recombinant protein (1 µg/ml in PBS 1X ...
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bioRxiv - Immunology 2021Quote: ... NGM plates were prepared with 1.7% Ultrapure agarose (ThermoFisher Scientific) in place of agar ...
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bioRxiv - Microbiology 2020Quote: ... untreated Nunc 96-wells round bottom polystyrene plates (Thermo Scientific), and Breathe-Easy sealing membranes (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were detached from their plates using Accutase (Thermo Fisher). 293T (ATCC ...
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bioRxiv - Microbiology 2021Quote: ... 96-well flat bottom Immulon 4 HBX plates (ThermoFisher Scientific) were coated with 150 μl/well of fetuin (Sigma Aldrich ...
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bioRxiv - Evolutionary Biology 2021Quote: ... protein A coated black 96-well plate (Pierce, Thermo Scientific) was washed thrice with TBS containing 0.05% Tween 20 (TBS-T ...
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Reconstitution of prospermatogonial specification in vitro from human induced pluripotent stem cellsbioRxiv - Developmental Biology 2020Quote: ... hiPSCs were plated at a density of 4-5×104 cells/cm2 onto a human fibronectin (Millipore)-coated 12 well plate in GK15 medium (GMEM [Life Technologies] with 15% KSR ...