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Citations for Thermo Fisher :
751 - 800 of 10000+ citations for PCNA Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... phagosomes were washed 3X with MAB and incubated with secondary antibodies against mouse (Alexa488; A11029, Thermo Fisher Scientific) and rabbit (Alexa647 ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were pre-incubated with 0.5 μg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience) in 100 μL of PBS with 2% FCS for 15 minutes at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... The bound mAb was detected with adding 100 μl/well of 3,3′,5,5′-Tetramethylbenzidine (TMB) substrate (Life Technologies) and incubation at room temperature for 5 min prior to the addition of 100 μl of 3% H2SO4 to stop the reaction ...
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bioRxiv - Microbiology 2021Quote: ... The isotyping of the selected clones was performed using the commercial Rapid ELISA Mouse mAb Isotyping kit (ThermoFisher). The kit determines the presence of the murine isotypes IgG1 ...
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bioRxiv - Immunology 2020Quote: ... cells were pre-incubated with 0.5 μg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience) in 100 μL of PBS with 2% FCS for 15 minutes at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... we obtained the sequence information of the 14.4.4 mAb VH and VL chain by isolating total mRNA from a 14.4.4 mAb-producing hybridoma cell line with the use of TRIzol (Invitrogen) followed by reversely transcribing the isolated mRNA into cDNA (First-strand cDNA Kit ...
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bioRxiv - Microbiology 2023Quote: ... Blots were then incubated with mouse anti-human E-cad ectodomain mAb (1:5000) (HECD-131700, Invitrogen, USA) for 2 h ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated for 1 h in anti-HA 2-2.2.14 IgG1 mouse mAb (ThermoFisher Catalog# 26183; RRID:AB_10978021) diluted 1:2000 and following 3 x 10 min washes in 0.1 M PB ...
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bioRxiv - Immunology 2023Quote: ... mAbs or ACE2 protein were first biotinylated using the EZ-Link Sulfo-NHS-Biotinylation Kit (Thermo Fisher Scientific) followed by removal of unreacted biotinylation reagents using a Zeba Spin Desalting Column (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... double immunofluorescence labeling with detergent was performed on 50-µm sections with the anti-pTau mAb AT8 (ThermoFisher) plus the anti-mouse IgG H + L secondary antibody conjugated with Alexa Fluor 488 (ThermoFisher) ...
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bioRxiv - Cancer Biology 2023Quote: ... naïve CD8+ T cells were stimulated with αCD3 and αCD28 mAb-coated dynabeads (Thermo Fisher Scientific, no. 11452D).
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bioRxiv - Microbiology 2024Quote: ... mAb pool was dialyzed in PBS pH 7.4 using Slide-A-Lyzer G2 Dialysis Cassette 3.5K (Thermo Scientific) overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-GFP rabbit (A11122, Invitrogen, CA, USA), chicken anti-GFP (AB16901 ...
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bioRxiv - Immunology 2021Quote: ... mouse serum samples were added to rat anti-mouse IgE mAb-coated 96-well Nunc MaxiSorb plates (Thermo Scientific). An HRP-conjugated goat anti-mouse IgE antibody (SouthernBiotech ...
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bioRxiv - Microbiology 2019Quote: Monoclonal antibodies (mAbs) were serially diluted 1:2 across white 96-well Nunclon© plates (Thermo Fisher Scientific, UK) in 50 μl of DMEM ...
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bioRxiv - Bioengineering 2019Quote: STP and mAb binding to the carriers was quantified indirectly using a bicinchoninic acid assay (BCA) kit (Thermo Fisher) according to the manufacture’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Mouse mAb anti-ZO-1 (clone ZO1-1A12) used in immunofluorescence 1:300 on zebrafish embryos was from Invitrogen. Rat mAb anti-HA High Affinity (clone 3F10) ...
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bioRxiv - Microbiology 2020Quote: ... The resin was subsequently washed with 3 resin volumes (RV) of the same buffer before mAb P3.2 (Thermo Fisher) was eluted using 3 RV of 0.2 M glycine pH 2.5 ...
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bioRxiv - Microbiology 2021Quote: ... COV2-2196 and COV2-2130 mAbs were produced after transient transfection using the Gibco ExpiCHO Expression System (ThermoFisher Scientific) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Non-specific staining was minimized by pre-incubating cells with 0.5 μg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience Thermo Fisher) in 100 μL of 1×PBS with 2% FCS for 15 min at 4 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... used at 1:5,000 in MAB buffer (100 mM Maleic acid, 150 mM NaCl, pH7.5) + 10% heat-inactivated lamb serum (Gibco 16070096) + 2% blocking reagent (Sigma 11096176001) ...
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bioRxiv - Microbiology 2020Quote: ... all mAbs were made as IgG1 and expressed in Chinese hamster ovary cells (ExpiCHO; Thermo Fisher Scientific, Waltham, MA). The mAbs were purified using HiTrap Protein A HP column (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... Plates were washed with TBS twice before incubating with serially diluted mAbs in casein blocking buffer (Thermo Fisher Scientific) for 90 min ...
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bioRxiv - Immunology 2020Quote: ... PE-Cy7-conjugated anti-human CD196 (CCR6) and PE-Cy7-conjugated anti-human MAVS mAbs were purchased from ThermoFisher Scientific (Waltham ...
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bioRxiv - Immunology 2020Quote: ... and α-CD28 (clone 9.3) mAbs at a concentration of 1 μg/ml in RPMI medium 1640 (Life Technologies) supplemented with 10% FCS and 1% penicillin/streptomycin (Gibco ...
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bioRxiv - Cell Biology 2022Quote: Endogenous TGF-βRII was probed using by two monoclonal antibodies (mAbs: sc-17792, Santa Cruz, and PA5-36115, ThermoFisher). Neither detected endogenous protein in our immunofluorescence or western blot studies.
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bioRxiv - Immunology 2022Quote: ... The supernatant containing secreted mAb was harvested at day 4 and purified using protein A-agarose beads (Thermo Fisher) as detailed previously56.
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bioRxiv - Molecular Biology 2022Quote: ... Certain mAbs were loaded with 50 μL/well to 96 well microplates (Thermo fisher maxisorp, catlog No: M9410-1CS) and incubated overnight After three washes with PBS-T (PBS with 0.05% Tween 20) ...
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bioRxiv - Immunology 2023Quote: ... Non-specific staining was minimized by pre-incubating cells with 0.5 µg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience Thermo Fisher) in 100 µL of 1×PBS with 2% FCS for 15 min at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... directed against the cytoplasmic domain of E-cad and a monoclonal anti-Occludin mouse mAb (331500, Life Technologies SA) and revealed using an anti-mouse IgG (H+L ...
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bioRxiv - Immunology 2024Quote: ... mAbs binding to the S proteins were detected using 25 µl/well of PNPP (p-nitrophenyl phosphate; Thermo Fisher) and the reaction was measured at a wavelength of 405nm using the Varioskan Lux Reader (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... The mAbs were detected using Alexa Fluor-594 conjugated goat anti-mouse IgG (H+L) (Invitrogen Cat# A-11032) and the reference Ab with an Alexa Fluor-488 conjugated goat Ab against the appropriate host (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... Recombinant baculovirus was generated using the Bac-to-Bac baculovirus expression system (Fisher Scientific) and was produced in Sf9 cells ...
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bioRxiv - Immunology 2021Quote: ... A recombinant bacmid DNA was generated using the Bac-to-Bac system (Life Technologies). Baculovirus was generated by transfecting purified bacmid DNA into Sf9 cells using FuGENE HD (Promega) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... linearized recombinant pCS2+ plasmids using the mMESSAGE mMACHINE SP6 transcription kit (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2020Quote: ... A recombinant bacmid DNA was generated using the Bac-to-Bac system (Life Technologies). Baculovirus was generated by transfecting purified bacmid DNA into Sf9 cells using FuGENE HD (Promega) ...
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bioRxiv - Neuroscience 2021Quote: ... supplemented with 20 ng/mL human recombinant basic fibroblast growth factor (bFGF; Invitrogen, USA), 20 ng/mL epidermal growth factor (EGF ...
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bioRxiv - Microbiology 2019Quote: ... Recombinant antibody was labelled with Alexa Fluor 594 following the manufacturer’s instructions (Life technologies). Standard molecular biology techniques were used to introduce a cysteine residue at position 239 in the CH2 domain of each heavy chain (44) ...
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bioRxiv - Microbiology 2019Quote: ... and 5 μg.L−1 of epidermal growth factor (EGF) human recombinant (Thermo Fisher Scientific) (Wu et al ...
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bioRxiv - Genetics 2019Quote: ... cells were incubated in differentiation medium (DM) (10 µg/ml recombinant human insulin (Gibco), 2% HI horse serum (Thermo Fisher) ...
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bioRxiv - Cell Biology 2021Quote: ... recombinant human insulin-like growth factor 1 (hIGF1; 100 ng/mL; Thermo Fisher Scientific), holo-transferrin (200 µg/mL ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant baculovirus was produced using the Bac-to-Bac Baculovirus expression system (Thermo Fisher). The mouse utrophin (NP_035812 ...
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bioRxiv - Bioengineering 2021Quote: Recombinant baculovirus were generated by using a Bac-to-Bac expression system (Invitrogen, USA) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... Gibco™ FGF-Basic AA 1-155 recombinant human protein (bFGF, PHG0264, Fisher Scientific), endothelial cell basal medium 2 (EGM 2 ...
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bioRxiv - Biophysics 2021Quote: ... The recombinant protein was expressed in Expi 293 GnTI- (Thermo Fisher, Waltham, MA, USA) at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... Recombinant cells were selected using 150 μg/ml of Hygromycin B (Invitrogen, Paisley, U.K.).
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bioRxiv - Cell Biology 2021Quote: Purified recombinant GST-tagged CDK1-cyclin A2 and His6-tagged CDK1-cyclin B1 (Invitrogen) were stored in 150 mM NaCl ...
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bioRxiv - Cancer Biology 2022Quote: ... or by tryptic shaving of cell-surface proteins by using recombinant enzymes (TrypLE, Gibco). Three biological replicates of cells cultured in the presence or absence of serum ...
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bioRxiv - Biochemistry 2022Quote: ... recombinant baculoviruses were generated according to the Bac-to-Bac Baculovirus expression system (Invitrogen). 2 ml of P3 virus were used to infect 1 L of Sf9 insect cells at a density of 1.1×106 ml-1 ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant baculoviruses were generated through the Bac-to-Bac system (ActRIIA; Invitrogen - Waltham, MA) or the Baculogold system (ActRIIB ...