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Citations for Thermo Fisher :
701 - 750 of 10000+ citations for PCNA Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... supernatants or serial dilutions of mouse IL2 recombinant protein (Peprotech, ThermoFisher Scientific) (100 μL/well ...
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bioRxiv - Neuroscience 2020Quote: ... anti-IFN-γ neutralizing mAb and/or an isotype IgG1 (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) were intraperitoneally injected daily to mice from PND5 to PND10 ...
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bioRxiv - Microbiology 2022Quote: ... The V5 epitope was detected with mouse mAb anti-V5 (diluted 1:1000) (Invitrogen, catalong no. R96025). Toxoplasma-specific antibodies include mouse mAb m-IMC1 (diluted 1:500 ...
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bioRxiv - Microbiology 2021Quote: ... anti-Utr2 mAbs or a commercially sourced anti-Candida mouse IgG2a monoclonal antibody (MA1-7009) (Fisher Scientific) in pre-warmed supplemented CO2-independent medium and incubated at 37 °C with gentle shaking for 40 min.
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bioRxiv - Biochemistry 2021Quote: ... The endoplasmic reticulum was stained using an Alexa488-coupled anti-calnexin (1:200, mAb AF18, ThermoFisher Scientific) in 10% goat serum overnight at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... The concentration of the purified mAb was determined using the Coomassie Plus protein assay reagent (Thermo Scientific).
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bioRxiv - Microbiology 2021Quote: ... Oregon green 488 was first conjugated to mAb 18B7 using Oregon Green 488 Protein Labeling Kit (ThermoFisher). BMDMs were plated at a density of 1.25 × 105 cells/well on 24-well plate with 12 mm circular coverslip ...
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bioRxiv - Immunology 2021Quote: ... The following primary antibodies (Ab) were used: occludin (mouse monoclonal [mAb], clone OC-3F10, Thermo Fisher Scientific), TFF3 (rabbit polyclonal [pAb] ...
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bioRxiv - Microbiology 2022Quote: Anti-ORF8 mAb (#1-3-2) was coupled to aldehyde/sulfate latex beads (Thermo Fisher Scientific A37304). The antibody was mixed with the latex beads and shaken at room temperature overnight ...
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bioRxiv - Biophysics 2024Quote: ... The 3 mAbs were specifically conjugated to HRP using EZ-Link™ Plus Activated Peroxidase Kit (ThermoFisher) and detected with ECL (Pierce ...
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bioRxiv - Microbiology 2023Quote: ... Blots were then incubated (1:500 dilution) with mouse anti-human E-cad mAb (4A2C7, Life Technologies) and anti-human HtrA (PA523395 ...
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bioRxiv - Microbiology 2023Quote: ... they were stained with a mastermix of anti-cytokine/transcription factor mAbs containing BCL6 PE (ThermoFisher Scientific) at 4 °C for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: The following primary antibodies were used: anti-ezrin mouse monoclonal antibody (mAb) (1:500; MA5-13862, Invitrogen); anti-p75 NTR rabbit mAb (1:1000 ...
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bioRxiv - Immunology 2023Quote: ... Antibodies were produced at MAB Discovery GmbH using the FreeStyle™ 293 expression system from Thermo Fisher. Transfected HEK-293 FreeStyle™ cells were incubated for 10-11 days at 37°C ...
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bioRxiv - Immunology 2023Quote: ... The assay for MUC5AC was performed using biotin-conjugated mouse anti-MUC5AC mAb (45M1, Invitrogen, #MA5-12175) as described previously (12) ...
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bioRxiv - Cancer Biology 2023Quote: ... the TCO- or Tz-conjugated mAbs were then labeled with Alexa Fluor 488 (Thermo Fisher Scientific, A2042), Alexa Fluor 594 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were incubated with goat anti-mouse Alexa Fluor 488 mAb secondary reagent (ThermoFisher Cat No. A11001) for at least 30 minutes ice ...
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bioRxiv - Microbiology 2024Quote: ... rabbit (goat anti-rabbit IgG, Invitrogen), sheep (rabbit anti-sheep IgG ...
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bioRxiv - Biochemistry 2021Quote: ... Primary Strep-MAb classic chromeo-555 (IBA) and secondary Alexa-fluor-488 or −555 goat anti-mouse (Invitrogen) were applied sequentially with PBS washes in between ...
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bioRxiv - Bioengineering 2021Quote: ... cells were pre-incubated with 0.5 µg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience) in 100 µL of PBS with 2% FCS for 15 minutes at 4 °C ...
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Dimeric prion protein ligand activates Adgrg6 but does not rescue myelinopathy of PrP-deficient micebioRxiv - Neuroscience 2020Quote: ... Briefly, sheep-anti mouse IgG paramagnetic beads (Dynal, 11201D) were coupled with anti-His mAb (Invitrogen, 37-2900) in coating buffer (PBS plus 0.1% immunoglobulin-free BSA ...
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bioRxiv - Immunology 2021Quote: ... or with unconjugated 4.3.1.3 mouse MAb and secondary detection with goat anti-mouse Alexa-Fluor 647 (A-21235; Life Technologies). Immunofluorescence was used to boost GFP signal in TgBAC(lck:gfp)vcc6 specimens with a chicken anti-GFP primary (Abcam ...
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bioRxiv - Biophysics 2022Quote: ... phagosomes were washed 3X with MAB and incubated with secondary antibodies against mouse (Alexa488; A11029, Thermo Fisher Scientific) and rabbit (Alexa647 ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were pre-incubated with 0.5 μg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience) in 100 μL of PBS with 2% FCS for 15 minutes at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... The bound mAb was detected with adding 100 μl/well of 3,3′,5,5′-Tetramethylbenzidine (TMB) substrate (Life Technologies) and incubation at room temperature for 5 min prior to the addition of 100 μl of 3% H2SO4 to stop the reaction ...
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bioRxiv - Microbiology 2021Quote: ... The isotyping of the selected clones was performed using the commercial Rapid ELISA Mouse mAb Isotyping kit (ThermoFisher). The kit determines the presence of the murine isotypes IgG1 ...
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bioRxiv - Immunology 2020Quote: ... cells were pre-incubated with 0.5 μg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience) in 100 μL of PBS with 2% FCS for 15 minutes at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... we obtained the sequence information of the 14.4.4 mAb VH and VL chain by isolating total mRNA from a 14.4.4 mAb-producing hybridoma cell line with the use of TRIzol (Invitrogen) followed by reversely transcribing the isolated mRNA into cDNA (First-strand cDNA Kit ...
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bioRxiv - Microbiology 2023Quote: ... Blots were then incubated with mouse anti-human E-cad ectodomain mAb (1:5000) (HECD-131700, Invitrogen, USA) for 2 h ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated for 1 h in anti-HA 2-2.2.14 IgG1 mouse mAb (ThermoFisher Catalog# 26183; RRID:AB_10978021) diluted 1:2000 and following 3 x 10 min washes in 0.1 M PB ...
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bioRxiv - Immunology 2023Quote: ... mAbs or ACE2 protein were first biotinylated using the EZ-Link Sulfo-NHS-Biotinylation Kit (Thermo Fisher Scientific) followed by removal of unreacted biotinylation reagents using a Zeba Spin Desalting Column (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... double immunofluorescence labeling with detergent was performed on 50-µm sections with the anti-pTau mAb AT8 (ThermoFisher) plus the anti-mouse IgG H + L secondary antibody conjugated with Alexa Fluor 488 (ThermoFisher) ...
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bioRxiv - Cancer Biology 2023Quote: ... naïve CD8+ T cells were stimulated with αCD3 and αCD28 mAb-coated dynabeads (Thermo Fisher Scientific, no. 11452D).
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bioRxiv - Microbiology 2024Quote: ... mAb pool was dialyzed in PBS pH 7.4 using Slide-A-Lyzer G2 Dialysis Cassette 3.5K (Thermo Scientific) overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... Human mAbs were expressed in ExpiCHO-S™ cells using an ExpiCHO™ Expression System Kit (Gibco, A29133). IgG were purified from cell culture supernatant 6-7 days after transfection via Protein A affinity chromatography using 2 mL or 5 mL column HiTrap MabSelect SuRe Protein A column (GE Healthcare ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were harvested with Accutase then incubated with anti-Ki67/FITC antibody (ThermoFisher, mouse mAb, clone 7B11, #MHKI6701) and stained with propidium iodide.
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bioRxiv - Microbiology 2024Quote: ... mAbs were incubated at 37□ for 5 h with 100 µL papain immobilized on agarose resin (ThermoFisher Scientific) in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... and recombinant SHH was purified with Detoxi-Gel™ Endotoxin Removing Gel (ThermoFisher) and dialyzed ...
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bioRxiv - Plant Biology 2020Quote: All recombinant plasmids were generated by the GATEWAY cloning system (Invitrogen, http://www.invitrogen.com/). The primers used for gene amplification are listed in S3 Table ...
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bioRxiv - Developmental Biology 2021Quote: ... Recombinant lentivirus was produced by transfecting HEK293T cells with Lipofectamine 2000 (Invitrogen, 11668019) or TransIT-2020 transfection reagent (Mirus ...
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bioRxiv - Biochemistry 2022Quote: Recombinant proteins were labeled with Alexa Fluor(tm) 647 NHS ester (Thermo Fisher) as described previously18 ...
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bioRxiv - Biochemistry 2022Quote: ... the recombinant protein mixtures were combined with 12.5 μM diFMUP (Life Technologies, E12020), added to 384-well plates ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant adenovirus plasmids were homologously recombined with pAd/CMV/V5-DEST vectors (Invitrogen), as per manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2021Quote: ... and 50 ng/ml human recombinant M-CSF (ThermoFisher Scientific, Rockford, IL, USA). After six days of PBMC culture ...
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bioRxiv - Developmental Biology 2020Quote: ... Recombinant lentiviruses were produced by transient transfection in HEK293FT cells (Invitrogen, CA, USA), as described earlier 18 ...
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bioRxiv - Genomics 2019Quote: ... 4) We used RNase Inhibitor in place of RNaseOUT Recombinant Ribonuclease Inhibitor (Invitrogen) in the first-strand RT reaction ...
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bioRxiv - Immunology 2019Quote: Chimeric VLPs or monomeric recombinant proteins were coated onto Maxisorp microtiter plates (Nunc) at 1μg/ml in PBS and incubated overnight at 4°C ...
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LIR-dependent LMX1A/LMX1B autophagy crosstalk shapes human midbrain dopaminergic neuronal resiliencebioRxiv - Cell Biology 2019Quote: ... the recombinant proteins were dialyzed using Slide-A-Lyzer™ cassettes (Thermo Fisher) into cold coupling buffer containing 100mM NaHCO3 and 500mM NaCl ...
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bioRxiv - Microbiology 2021Quote: ... 10 ng/ml of recombinant human fibroblast growth factor 2 (FGF2, Life Technologies), 20 ng/ml of recombinant human epidermal growth factor (EGF ...
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bioRxiv - Cell Biology 2020Quote: ... except that a final concentration of 10 nM recombinant PKR (Life Technologies # PV4821) and 1 mM ATP was added during incubation with FLAG M2 magnetic beads ...