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Citations for Thermo Fisher :
751 - 800 of 10000+ citations for P450 Demethylation Activity Kit 2 plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... confluent Vero cells grown in 24-well plates with coverslips were transfected with 2 μg of pCS2-6xHis-ubiquitin plasmid DNA using Lipofectamine 2000 (Invitrogen, 11668-019) for 6 h in Opti-MEM (Gibco ...
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bioRxiv - Microbiology 2020Quote: ... an early log phase promastigote (2 × 106 promastigotes per ml) were seeded in 96-well flat-bottom plastic tissue-culture plates (Nunc Int., Denmark) and incubated at 22°C for 72 hours in presence or absence of compounds ...
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Genomic and phylogenetic analyses of SARS-CoV-2 strains isolated in the city of Gwangju, South KoreabioRxiv - Microbiology 2020Quote: ... the cells were seeded in 24-well plates at 2×104 cells/well and cultured in modified Eagle’s medium (MEM; Gibco, Thermo Fisher Scientific, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2022Quote: ... Pellets from six-well plates were resuspended in 50 μl of 1× DNase buffer and incubated with 2 units of DNase (TURBO; Thermo Fisher Scientific) at 37 °C for 1 h ...
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bioRxiv - Cancer Biology 2022Quote: CHO-K1 cells stably transfected with nLuc-PAR1/2-eYFP constructs were plated in a 96-well cell culture plate (polystyrene, flat-clear bottom, Nunclon Delta, Nunc, ThermoFisher Scientific) at a cell density of 1 × 104 cells per well and cultured for 48 h in F-12 complete culture medium ...
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bioRxiv - Microbiology 2022Quote: ... Roughly 1/3 of the plate (2-3 loopfuls) were added to 400 µl 1x Tris-Ethylenediaminetetraacetic acid (EDTA) pH 7.5 (Thermo Scientific, Waltham, USA) and heated at 80 ºC for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... Neurons were seeded at 50×103 per cm2 on plates coated with poly-L-ornithine and laminin in Neurobasal Plus medium containing 2% B27 Plus supplement (Life Technologies, USA). On day 2 ...
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bioRxiv - Microbiology 2023Quote: Cells from a log-phase culture were loaded into an ONIX B04A plate and perfused with fresh LB and 2 µg/mL FM 4-64 (Invitrogen, Cat. #T13320) for 1 h ...
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bioRxiv - Neuroscience 2022Quote: ... P-1149)-coated 96-well and 24-well plates were seeded with 4 × 104 and 2 × 105 cells accordingly in neurobasal medium (Invitrogen, 21103-049) supplemented with 10% heat-inactivated FBS (Gibco) ...
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bioRxiv - Molecular Biology 2023Quote: Artificial muscles were mounted on PDMS posts placed into a 24 well plate and incubated with 2 ml Opti-MEM basal media without phenol red containing Fluo-4AM (Thermo scientific, F14201) as per manufacturer’s instructions for 30mins ...
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bioRxiv - Microbiology 2023Quote: Pseudotyped viruses were titrated by serially diluting filtered pseudotypes with fresh DMEM starting from a 1:2 to a final 1:512 dilution using white 96-well flat bottom plates (ThermoFisher, Woolwich, UK). Then appropriate target cells were added (Table 1 ...
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bioRxiv - Neuroscience 2023Quote: ... larvae were visually screened for the expression of single or sparsely labelled FoxP2.A:FingR(PSD95)+ neurons in the tectum using a 20x water-immersion objective and an LSM 980 confocal microscope with Airyscan 2 (Zeiss) and placed into individual wells of a 6-well plates (Thermo Fisher Scientific) to keep track of individual larvae and the corresponding labelled neurons ...
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bioRxiv - Immunology 2023Quote: ... 200-500 μm cystic EBs were selected under a dissecting microscope and transferred onto adherent tissue culture plates (∼2.5 EBs/cm2) for cultivation in Hematopoietic Differentiation (HD) medium (APEL 2, Stem Cell Tech, 05270; Protein Free Hybridoma, Thermo Fisher, 12040077 ...
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bioRxiv - Biochemistry 2023Quote: Sample digests were acidified with formic acid to a pH of 2-3 prior to desalting using C18 solid phase extraction plates (SOLA, Thermo Fisher Scientific). Desalted peptides were dried in a vacuum-centrifuged and reconstituted in 0.1% formic acid for LC-MS analysis.
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bioRxiv - Cell Biology 2023Quote: ... was placed into 24-well plates and coated for 2 hours with 100 µL of 50 µg/mL poly-D-lysine (Gibco #A38904-01) in the 37°C tissue culture incubator ...
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bioRxiv - Cell Biology 2023Quote: ... cells were transfected with 2 micrograms of either CELSR constructs or empty vector DNA in a 6-well plate (Fisher Scientific FB012927) using a 1:3 mixture of LipoD293 (SignaGen Laboratories SL100668) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were passaged 1:4 into new six-well plates with complete DMEM containing 2 μg mL-1 puromycin (Thermo Fisher Scientific). After selection for two weeks ...
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bioRxiv - Neuroscience 2023Quote: ... The samples then underwent solid-phase extraction cleanup with an Oasis HLB plate (Waters) and 2 μL of each sample was injected onto a QExactive HF mass spectrometer (Thermo Fisher Scientific) coupled to an Ultimate 3000 RSLC-Nano liquid chromatography system (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... Primary mouse B cells were resuspended in the supplemented XF DMEM media to a concentration of 4×106 cells/mL while BJAB cells were resuspended at a concentration of 2×106 cells/mL and applied to assay plates coated with Poly-D-Lysine (Gibco A38904-01) or CellTak (BD Biosciences).
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bioRxiv - Biochemistry 2024Quote: ... was placed into 24-well plates and coated for 2 hrs with 100 µL of 50 µg/mL poly-D-lysine (Gibco #A38904-01) in the 37 °C tissue culture incubator ...
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bioRxiv - Biophysics 2022Quote: ... plate reader using 96-well NUNC optical bottom plates (Thermo Scientific). The total sample volume used was 100 μL for all the measurements ...
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bioRxiv - Bioengineering 2019Quote: Plate sorting was conducted using 96-well optical plates (Fisher Scientific) or qPCR plates (Biorad ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The plate was sealed with a plate film (Thermo Fisher 4311971) and centrifuged for 5 minutes at 500 x g at room temperature ...
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bioRxiv - Bioengineering 2021Quote: 96-well ELISA plates (Nunc MaxiSorp flat-bottom plates, Thermo Fisher) were coated with 10 nM RBD ...
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bioRxiv - Bioengineering 2023Quote: ... ELISA plates (MaxiSorp 96 well plates, Thermo Fisher #12-565-135) were coated overnight at room temperature with influenza A/Puerto Rico/8/34 virus particles (400 HAU/ml ...
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bioRxiv - Synthetic Biology 2023Quote: ... The plate was covered with an adhesive plate seal (Thermo Scientific) and loaded measured on a VARIOSKAN LUX (Thermo Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... The plate was sealed with a plate sealing film (Thermo Scientific) and incubated at 42 °C in a BMG FLUOstar Omega plate reader for 100 h with cycles of 1 min shaking (400 rpm double orbital ...
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bioRxiv - Plant Biology 2024Quote: ... the plates were sealed with Adhesive PCR plate foil (ThermoFisher AB0626) and immediately frozen on dry ice ...
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bioRxiv - Cancer Biology 2024Quote: Ninety-six well plates (Nunc MaxiSorp Flat-Bottom Plate, 44240421, Invitrogen) were coated with NeutrAvidin (2μg/ml ...
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bioRxiv - Bioengineering 2022Quote: Cell proliferation in T-SLICE zones was analyzed using an EdU (5-ethynyl-2’-deoxyuridine) Cell Proliferation Kit (Click-iT− EdU Cell Proliferation Kit for Imaging, Alexa Fluor™ 488 dye: Invitrogen). Cell culture media was supplemented throughout with 10 uM EdU ...
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bioRxiv - Bioengineering 2023Quote: Proliferative activity of 2D hydrogel cell cultures was measured using an EdU (5-ethynyl-2’-deoxyuridine) labeling kit (Click-iT™ EdU Cell Proliferation Kit for Imaging, Invitrogen). 5 μM EdU solution was dosed to cells 12 h prior to fixing with 4% paraformaldehyde ...
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bioRxiv - Microbiology 2021Quote: ... the purified PCR products were normalized to equimolar concentration with the SequalPrep Normalization Plate Kit (Thermo Fisher Scientific) and the DNA library was sequenced on the Illumina MiSeq next generation sequencing system (Illumina Inc. ...
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Hypoxia-mediated suppression of pyruvate carboxylase drives tumor microenvironment immunosuppressionbioRxiv - Cancer Biology 2022Quote: ... Labeled cDNA was hybridized to the plate using GeneTitan Hybridization Wash and Stain Kit for WT Arrays (Affymetrix).
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bioRxiv - Microbiology 2022Quote: ... The PCR products were normalized with the SequalPrep Normalization Plate Kit (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) and eluted in 20 μL elution buffer ...
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bioRxiv - Immunology 2023Quote: ... CpG content was measured in a 96 well plate using Ribogreen™ fluorescence staining kit from Thermo Fisher and a SpectraMax i3 plate reader (Molecular devices ...
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bioRxiv - Microbiology 2023Quote: ... Duplicate PCRs were combined prior to clean up and normalization with a SequalPrep Normalization Plate kit (ThermoFisher Scientific) following manufacturer instructions using 25 µL of PCR product per sample ...
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bioRxiv - Immunology 2023Quote: ... CpG content was measured in a 96 well plate using Ribogreen™ fluorescence staining kit from Thermo Fisher and a SpectraMax i3 plate reader (Molecular devices ...
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bioRxiv - Microbiology 2023Quote: ... triplicate first-step PCR reactions were pooled and purified and normalised with the SequalPrep Normalisation Plate Kit (Invitrogen) following the manufacturers’ instructions ...
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bioRxiv - Physiology 2023Quote: ... Protein concentration was quantified on a plate reader using the Pierce BCA protein assay kit (#23225, Thermo Scientific). Gel lanes were loaded with 30 μg of total protein ...
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bioRxiv - Microbiology 2020Quote: ... Peroxidase activity was visualized with SuperSignal West Pico PLUS Chemiluminescent Substrate (#34580, Thermo Fisher Scientific, USA).
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bioRxiv - Developmental Biology 2021Quote: ... endogenous peroxidase activity was quenched using 3% H2O2 (Cat No. H/1750/15, Thermo Fisher Scientific). Antigen retrieval was performed by treatment of tissue sections with porcine trypsin tablets (Cat No ...
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bioRxiv - Developmental Biology 2021Quote: ... normalized to β-galactosidase activity using Galacto-Star β-Galactosidase Reporter (T1012, Invitrogen, Carlsbad, CA, USA) as described (Chen et al. ...
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bioRxiv - Developmental Biology 2020Quote: ... normalized to β-galactosidase activity using Galacto-Star β-Galactosidase Reporter (T1012, Invitrogen, Carlsbad, CA, USA) as previous described (Chen et al. ...
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bioRxiv - Plant Biology 2020Quote: ... Detection of auto-acetylation activity was performed through immunoblotting using acetylated lysine monoclonal antibody (1C6) (Invitrogen).
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bioRxiv - Cell Biology 2021Quote: ... Sections were also stained to detect activity of tartrate-resistant acid phosphatase (1:80, Invitrogen, E6601A) and alkaline phosphatase (Vector Laboratories ...
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bioRxiv - Bioengineering 2020Quote: ... The metabolic activity of cell-seeded biomaterials was examined via an alamarBlue™ (Thermo Fisher Scientific) assay at days 1 ...
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bioRxiv - Cell Biology 2021Quote: Kinase activity assays were performed using the SelectScreen biochemical kinase profiling service provided by Life Technologies. Adapta screening protocols were used to measure PIK3C2A (PtdIns3K-C2α) ...
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bioRxiv - Microbiology 2020Quote: Metabolic activity was used as a measurement of cell viability by using Presto Blue reagent (Invitrogen). MDA-MB-231 and MDA-MB-436 cells were untreated or treated with DMSO ...
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bioRxiv - Cancer Biology 2022Quote: ... PP4 activity was assayed by incubating the washed beads with 100 µM DiFMUP substrate (#D6567, Invitrogen) and fluorescence measured at 450 nM after 60 minutes using FlexStation 3 (Molecular Devices) ...
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bioRxiv - Neuroscience 2023Quote: Efflux transporter activity was determined by cellular efflux of rhodamine 123 (10 µM, Thermo Fisher Scientific), Hoechst 33342 (5 µg/mL ...