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Citations for Thermo Fisher :
701 - 750 of 10000+ citations for P450 Demethylation Activity Kit 2 plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Purified amplicons were pooled in equimolar concentrations with a SequalPrep Normalization Plate Kit (Applied Biosystems, Cat#A1051001) and sequenced on Illumina MiSeq System (RRID:SCR_016379) ...
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bioRxiv - Microbiology 2020Quote: ... and then normalized using the high-throughput Invitrogen SequalPrep 96-well Plate Kit (Thermo Fisher Scientific Corp.). Samples were then pooled to make one library ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Indexed PCR products were purified and normalized using the SequalPrep Normalization Plate Kit (Invitrogen, Carlsbad CA, USA), subsequently quantified using the Qubit dsDNA HS Kit (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... 4 wells of each plate were transduced with the CytoTune-iPS 2.0 Sendai Reprogramming Kit (Thermo Fisher) using the recommended MOIs for each factor (KOS=5 ...
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Self-organised pattern formation in the developing neural tube by a temporal relay of BMP signallingbioRxiv - Developmental Biology 2023Quote: RNA was extracted from cells cultured in 12-well plates using the Purelink RNA Mini Kit (Invitrogen). DNA was removed by incubating the preparation on the column with Purelink DNase (Invitrogen) ...
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bioRxiv - Systems Biology 2024Quote: ... Amplicons from each 96-well plate were pooled and purified using the PCR Purification Kit (ThermoFisher K310002). Each pooled sample was prepared as a single sequencing library by the Molecular Biology Core Facilities at the Dana-Farber Cancer Institute ...
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bioRxiv - Plant Biology 2019Quote: ... HRP activity was detected with the super-signal west femto maximum sensitivity substrate (Thermo Scientific) and the Image Quant LAS4000 Mini device (GE Healthcare) ...
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bioRxiv - Immunology 2021Quote: ... The HRP activity was determined using the SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher) and scanned the blot using a ChemiDoc MP Imaging System (Bio-Rad).
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bioRxiv - Cell Biology 2021Quote: ... The metabolic activity of the cells was detected with Vario Skan Flash (Thermo Fisher Scientific) at 460 nm wavelength to compare the difference between control and siRNA knockdowns.
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bioRxiv - Microbiology 2022Quote: ... The activity of PYK was measured according to [63] in 0.1M TEA buffer (ThermoFisher Scientific) (final pH 7.6) ...
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bioRxiv - Bioengineering 2020Quote: ... Changes in metabolic activity were assessed using an MTT assay (Thermo Fisher Scientific, Waltham, MA). Hydrogels were each incubated with 20 µL MTT and 200 µL DMEM + 2% FBS for four hours ...
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bioRxiv - Bioengineering 2020Quote: ... Cell viability was determined by quantifying cellular metabolic activity using alamarBlue assay (Thermo Fisher Scientific). Metabolic activity for each group was normalized to the metabolic activity of cells treated with medium only ...
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bioRxiv - Molecular Biology 2021Quote: ... PLA1 and PLA2 activities of PlaF were measured using fluorescent substrates purchased from Thermo Fisher Scientific Inc ...
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bioRxiv - Neuroscience 2019Quote: Mechanotransduction channel activity was assessed through FM 1-43 dye uptake (Molecular Probes, Eugene, OR). A working stock of 3 µM FM 1-43 was used for a 30 second incubation and rinsed three times immediately with embryo water ...
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bioRxiv - Microbiology 2021Quote: β-lactamase activity was assessed by colorimetric assay using the chromogenic cephalosporin compound nitrocefin (Thermo Scientific) (O’Callaghan et al. ...
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bioRxiv - Microbiology 2021Quote: ... Cellular metabolic activity was assessed using a Vybrant MTT Cell Proliferation Assay (Thermo Fisher Scientific) following manufacturer’s instructions.
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bioRxiv - Genetics 2021Quote: ... Activities of LacZ and luciferase were measured using the Tropix Galacto-Star (Applied Biosystems, T1056) and Tropix Luc-Screen systems (Applied Bisosystems ...
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bioRxiv - Pathology 2021Quote: ... Liver injury was measured using serum ALT activity (Pointe Scientific ALT Assay by Fisher Scientific).
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bioRxiv - Immunology 2021Quote: ... HRP enzymatic activity was revealed using 3,3’,5,5’-tetramethylbenzidine (TMB) substrate solution (Thermo Fisher Scientific) for 5 min and was stopped by adding 1.2 M sulfuric acid ...
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bioRxiv - Molecular Biology 2022Quote: ... The Luciferase activities were measured using Luminoskan Ascent luminimeter (Thermo Fisher Scientific, Waltham, MA, USA). The excitation wavelength used was 365 nm and the emission wavelength was 455 nm ...
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bioRxiv - Immunology 2022Quote: ... Alexa Fluor 488 and caspase-3/7 activity detection dyes were purchased from Thermo Fisher, and Viobility™ 405/452 Fixable Dye from Miltenyi Biotec.
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bioRxiv - Bioengineering 2022Quote: Voltage activity within human heart organoids was assessed using di-8-ANEPPS (Thermo Fisher Scientific). Di-8-ANEPPS was solubilized in DMSO per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Cellular metabolic activity was assessed using a CyQUANT MTT Cell Proliferation Assay (Thermo Fisher Scientific) following manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: Phospholipase A1 and A2 activity were determined using commercial assays (Cat# E10217 and E10219, Thermofisher). The protocol for determining activity was based on the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Luciferase activity was measured according to the manufacturer’s protocol by using Varioskan LUX (Thermo Scientific). At least three independent measurements were performed for each setting.
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bioRxiv - Neuroscience 2024Quote: Functional activity in co-cultures and muscle monocultures was assessed by adding 50μM glutamate (Gibco) to cells previously plated in μ-Slide 8 Well (ibidi ...
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bioRxiv - Developmental Biology 2021Quote: Click-iT EdU (5-ethynyl-2’-deoxyuridine, a thymidine analog) kit from Life Technologies was used to perform DNA replication assay (Milton et al. ...
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bioRxiv - Microbiology 2019Quote: ... The Ion OneTouch 2 system using the Ion PGM Template OT200 Kit (Life Technologies) was used for template preparation which includes the steps of emulsification ...
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bioRxiv - Molecular Biology 2022Quote: ... corrected for the amount of total ERK using ERK1/2 (Total) ELISA Kit (Invitrogen) to measure ERK levels.
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bioRxiv - Immunology 2020Quote: ... 2 mg of RNA was reverse transcribed using the Maxima Reverse Transcriptase kit (ThermoFisher). RT2 profiler PCR array of human NF-κB signaling targets was performed as instructed by the manufacturer (Qiagen).
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bioRxiv - Cell Biology 2022Quote: ... (2) Osteogenic medium: StemProTM Osteogenesis Differentiation Kit from Life Technologies (Grand Island, NY, USA); (3 ...
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bioRxiv - Immunology 2023Quote: ... NeuroTrace™ 530/615 and diamidino-2-phenylindole (DAPI) (BrainStainTM Imaging Kit, Invitrogen #B34650). Sections were then thrice washed in PBT (15 min per wash ...
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bioRxiv - Immunology 2023Quote: ... 1.5 mL of enhancer 1 and 0.15 mL of enhancer 2 (Expifectamine kit, Gibco) were added to the cells ...
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bioRxiv - Developmental Biology 2020Quote: ... 2×105 cells/well (6-well plate) HCECs were transfected with 30pmol siRNA using lipofectamine RNAiMax (Life Technologies, Grand Island, NY, USA) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2020Quote: ... screen plates were washed 3x with FluoroBrite basal media (2 mM GlutaMAX and 10 mM HEPES in FluoroBrite DMEM (Thermo Fisher Scientific)) using a BioTek 406 plate washer with 10 min incubations followed by a 1 min centrifugation at 200 rcf to settle media between washes ...
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bioRxiv - Biochemistry 2020Quote: ... one well of a 6 well plate was transfected with 2 μg of plasmid DNA with Lipofectamine® 2000 (Life Technologies™) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... 0.2 ml of each sample or buffer were disposed in wells of black 96-well plates (Nunc F96 MicroWell, Thermo Fisher Scientific), maintained at 25°C and agitated at 1000 rpm ...
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bioRxiv - Cancer Biology 2020Quote: ... was diluted in SYTOX Green-containing DMEM from 2 mM to 50 μM in a dilution plate using a Versette liquid handler (Thermo Fisher Scientific). The day after seeding ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were subsequently plated on islands of glial cells at a density of 1.25 × 104 cells/cm2 in 6-well plates in Neurobasal-A supplemented with 2% B27 and 0.2% penicillin/streptomycin (Invitrogen/ Thermo Fisher, Waltham, MA). Lentiviral particles encoding synaptoPAC were added 1–4 days after plating ...
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bioRxiv - Microbiology 2020Quote: ... and THP-1 cells (100 μl of 2 x 105 cells/mL) were seeded in 96 well tissue culture plate (Nunc™, Thermofisher) for 24 hours using DMEM and RPMI media respectively supplemented with 5% FBS in 5% CO2 incubator at 37°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Organoids were imaged from day 1 to day 2 at room temperature directly in low binding 96-well plate (Nunclon Sphera U-Shaped Bottom Microplate, Thermo Fisher Scientific). The volume of the organoids was acquired with 2.24 µm z step size using 488 nm excitation laser at 10% intensity in 30 min intervals.
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bioRxiv - Biophysics 2020Quote: ... 0.2 ml of each sample and buffer were disposed in wells of black 96-well plates (Nunc F96 MicroWell, Thermo Fisher Scientific), maintained at 25°C and agitated at 1000 r.p.m ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... cells were seeded in poly-L-lysine/laminin coated 6-well plates at a density of 1.5 × 106 per well and maintained in Neurobasal medium containing B27 supplement (2%, Invitrogen, Grand Island, NY), penicillin (50 U/ml ...
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bioRxiv - Microbiology 2020Quote: ... plates were washed six times with PBS (200 μl) and then blocked for 1-2 hours with Goat Serum (ThermoFisher Scientific, 16210072) at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... P-1149)-coated 96-well and 24-well plates were seeded with 4 × 104 and 2 × 105 cells accordingly in neurobasal medium (Invitrogen, 21103-049) supplemented 5 ng/ml HBEGF (Sigma-Aldrich ...
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bioRxiv - Systems Biology 2020Quote: ... Clone cultures were concentrated to 20-fold in M9 buffer and 10 μL was plated onto a well of a 96 well plate containing NGM agar with 2 mM Isopropyl β-d-1-thiogalactopyranoside (IPTG, Fisher Scientific). Plates were dried and stored at room temperature ...
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bioRxiv - Bioengineering 2021Quote: ... transfected with 1 μg plasmids at 1050v/20ms/2 pulses and cultured in a 24-well Nunc cell culture plate (Thermo Fisher Scientific) at the density of 200,000/well in PRMI 1640 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: Approximately 2 x 106 cells in 1 mL DMEM10 were seeded into wells of 12-well cell culture plate (Nunc, NY, USA). Two hours later after the cells had adhered ...
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bioRxiv - Immunology 2021Quote: ... Plates were blocked with blocking buffer made up with 2% bovine serum albumin (BSA) Fraction V (Thermo Fisher Scientific, Waltham, MA, USA), 1% gelatin from bovine skin (Sigma-Aldrich ...
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bioRxiv - Genetics 2021Quote: ... Cox-2 and the 18S rRNA control was assessed through real-time PCR using TaqMan® Gene Expression Array Plates (ThermoFisher Scientific) containing predesigned ...