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Citations for Thermo Fisher :
751 - 800 of 10000+ citations for Human Bcl2 Associated Death Promoter BAD ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... signals of both ELISAs were visualized using 1-step Ultra TMB ELISA substrate (Fisher Scientific 34028), an incubation period at RT and addition of 2 M sulfuric acid as the stop reagent ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were collected 24 hr after treatment with the cell death inducers with TrypLE Express (Life Technologies) and resuspended in PBS containing 0.5% bovine serum albumin (BSA ...
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bioRxiv - Immunology 2022Quote: Neutrophil cell death or the presence of extracellular DNA was quantified using a Sytox Green-based (Invitrogen) fluorescence assay similar to previous descriptions 2,35 ...
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bioRxiv - Microbiology 2023Quote: ... a final 20,000-fold dilution of the “death dye” ToPro3 (Molecular Probes T-3605; Eugene, OR, USA) was added to each sample to distinguish between dead and live cells [65] ...
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bioRxiv - Cancer Biology 2023Quote: ... and ethidium homodimer-1 (EthD-1) was used as a marker of cell death (Invitrogen, Waltham, MA). 10X images were captured using a Nikon Ti-U Inverted Fluorescence Microscope and NIS-Elements F Package imaging software 3.22.00 Build 710 (Nikon Instruments Inc ...
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bioRxiv - Molecular Biology 2024Quote: ... Cell death was detected by propidium Iodide (PI) or SYTOXR Green (100 nM, Thermo Fisher Scientific, S7020) and analyzed by Cytation 5 (Agilent ...
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bioRxiv - Plant Biology 2024Quote: ... Cell death was measured with the fluorescent dye SYTOX Green (Ex/Em = 504/523 nm; ThermoFisher Scientific) that accumulates in dead cells only ...
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bioRxiv - Plant Biology 2024Quote: ... using a 7500 Fast Real-Time PCR System and the associated 7500/7500 software (Applied Biosystems). Relative expression levels were calculated according to the 2-ΔΔCt method 55 ...
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bioRxiv - Immunology 2023Quote: ... To remove surface associated DNA the EVs were incubated with 10 units DNase I (Thermo Scientific) for 1 hr at room temperature.
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bioRxiv - Microbiology 2024Quote: ... Lipid content associated with OMVs was determined using the lipophilic fluorescent dye FM4-64 (Thermofisher®) as described previously (76) ...
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bioRxiv - Physiology 2023Quote: ... Total IL-1β protein level was measured by mouse IL-1β/IL-1F2 DuoSet ELISA kit and first normalized to total protein level quantified by Pierce™ BCA Protein Assay Kit (Thermo Scientific), and then normalized to the sham group.
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bioRxiv - Microbiology 2020Quote: ... RNA was transcribed from PCR products encoding the desired bases downstream of a T7 promoter region using MEGAScript T7 Transcription Kit (Invitrogen, Carlsbad, CA) followed by purification using the MEGAClear Transcription Clean-Up Kit (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ssrSSa and sprB were in vitro transcribed from a PCR product template T7 promoter-containing (primers listed in Table S3) with MEGAscript T7 transcription kit (Thermo Fisher Scientific). RNAs were separated on 8 % polyacrylamide-7 M urea gel electrophoresis and eluted overnight in G50 elution buffer (20 mM Tris-HCl pH7.5 ...
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bioRxiv - Immunology 2020Quote: ... Digoxigenin (DIG)-labeled riboprobes were synthesized from T7 (for antisense probe synthesis) and T3 (for sense probe synthesis) promoters using MEGAscript Transcription kits (Ambion, Thermo Fisher Scientific) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2021Quote: The 5’-UTRs of transcripts were amplified from MIN6 cell cDNA with the addition of a T7 promoter and in vitro transcribed using the AmpliScribe™ T7-Flash™ RNA transcription kit (Ambion) and biotin-16-CTP (Roche ...
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Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeastbioRxiv - Genetics 2022Quote: ... where the PSMB7 alleles are under the control of the constitutive GPD (TDH3) promoter (Gateway® LR Clonase® II enzyme mix kit from Invitrogen). The conditions for the error-prone PCR were selected to introduce 1-4 mutations per Kbp.
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bioRxiv - Cell Biology 2024Quote: ... This linear DNA template containing the T7 promoter was used to transcribe sgRNAs with the MEGAshortscript™ T7 Transcription Kit (Invitrogen; AM1354). The synthesized gRNAs were precipitated with ethanol and dissolved in nuclease-free water.
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bioRxiv - Microbiology 2023Quote: ... Purified PCR fragments were ligated in pCR® II Vector – Dual Promoter TA Cloning Kit (Invitrogen-Thermo Fisher Scientific, Waltham, MA, USA) and subsequently used for E ...
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bioRxiv - Developmental Biology 2023Quote: ... A T7 promoter sequence was included in forward primer for in vitro transcription using the mMESSAGE mMACHINE T7 Ultra Transcription kit (Thermo Fisher Scientific). After LiCl precipitation ...
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bioRxiv - Microbiology 2024Quote: ... were amplified using T7 RNA polymerase promoter-flanked primers (Varjak et al. 2017; McFarlane et al. 2020) and dsRNAs produced with MEGAscript RNAi kits (Thermo Fisher Scientific) by in vitro transcription as per manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2021Quote: ... 96-well ELISA plates (Nunc, Germany) were coated with SARS-CoV-2 specific antigen (S1-RBD at a concentration of 1.5μg/well in PBS pH 7.4) ...
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bioRxiv - Immunology 2022Quote: MaxiSorp ELISA plates (Thermo Fisher Scientific) were coated with 2 μg/ml purified RBD or S protein in 1xBBS (140 mM NaCl ...
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FOXO dictate initiation of B cell development and myeloid restriction in common lymphoid progenitorsbioRxiv - Immunology 2022Quote: ... MaxiSorp ELISA plates (Thermo Fisher Scientific) were coated with 10-20µg/ml of anti-mouse immunoglobulin (Ig ...
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bioRxiv - Microbiology 2022Quote: ... High-binding ELISA plates (Thermo Scientific) were coated with capture antibody and incubated overnight at room temperature ...
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bioRxiv - Immunology 2021Quote: ... ELISA plate wells (Thermo Fisher Scientific) were coated with VISTA-Fc (1 µg/ml ...
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bioRxiv - Microbiology 2021Quote: ... ELISA plates (NUNC Maxisorp, Thermo Scientific) were coated with 100 μl of recombinant RBD protein or M2e peptide (0.75 μg/ml or 0.5 μg/ml ...
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bioRxiv - Microbiology 2021Quote: ... 96-well ELISA plates (Nunc Maxisorp) were coated with 300 ng human IgM (CP initiator ...
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bioRxiv - Microbiology 2021Quote: ... ELISA plates (NUNC Maxisorp, Thermo Scientific) were coated with 100 μl of recombinant RBD protein or M2e peptide (0.75 μg/ml or 0.5 μg/ml ...
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bioRxiv - Microbiology 2021Quote: ... ELISA plates (NUNC Maxisorp, Thermo Scientific) were coated with 100 µl of M2e peptide or rHA (H1 ...
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bioRxiv - Microbiology 2021Quote: ... ELISA plates (NUNC Maxisorp, Thermo Scientific) were coated with 100 µl of M2e peptide or rHA (H1 ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... Ultra TMB-ELISA Substrate Solution (ThermoFisher) was incubated for 15 to 25 min for color to develop on the plate ...
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bioRxiv - Microbiology 2022Quote: ... 96-well ELISA plates (Nunc, Germany) were coated with SARS-CoV-2 specific antigens (S1-RBD at a concentration of 1.5μg/well ...
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bioRxiv - Microbiology 2020Quote: 96-well ELISA plates (Thermo Fisher) were coated with 1ug of recombinant proteins (20 mM NaHCO3/Na2CO3 ...
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bioRxiv - Immunology 2020Quote: ... MaxiSorp high binding ELISA plates (Nunc) were coated with 100 μL/well of 1 μg/mL recombinant SARS-CoV-2 protein in PBS ...
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bioRxiv - Immunology 2021Quote: ... 96 well ELISA plates (Thermo Fisher) were coated with goat anti-human Ig (10 μg/ml ...
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bioRxiv - Immunology 2021Quote: ... ELISA plates (NUNC, Thermo Fisher Scientific) were coated with 1 μg MOG35-55 resuspended in coating buffer (0.05M carbonate buffer (pH 9.6) ...
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bioRxiv - Immunology 2021Quote: ... ELISA plates (NUNC, Thermo Fisher Scientific) were coated with 1 μg MOG35-55 resuspended in coating buffer (0.05M carbonate buffer (pH 9.6) ...
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bioRxiv - Microbiology 2021Quote: ... MaxiSorp ELISA plates (NUNC, Rochester, NY) were coated with 100 ng of recombinant fusion protein (rF1-V ...
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bioRxiv - Microbiology 2020Quote: ... ELISA plates (MaxiSorp Nunc-immuno plates) were coated with 1 μg/ml of anti-CH3 (MCA878G ...
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bioRxiv - Microbiology 2020Quote: ... ELISA plates (MaxiSorp Nunc-Immuno plates) were coated with 1 μg/ml recombinant RBD-His protein antigen (Cat ...
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bioRxiv - Microbiology 2021Quote: ... Streptavidin coated ELISA plates (Thermo Scientific) were incubated with 8 μg/ml G-H loop peptide diluted in PBS at 37°C for 2 hours ...
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bioRxiv - Immunology 2021Quote: ... MaxiSorp high binding ELISA plates (Nunc) were coated with 100 μL per well of 1 μg mL−1 recombinant SARS-CoV-2 protein with the pre-fusion stabilized conformation in PBS ...
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bioRxiv - Neuroscience 2021Quote: ELISA plates (96-well; NUNC, Denmark) were incubated overnight at room temperature with capture antibody (R&D Systems ...
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bioRxiv - Immunology 2020Quote: ... In brief: MaxiSorp ELISA plates (Nunc) were coated with 50ng/well (100μl at 0.5 μg/ml ...
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bioRxiv - Immunology 2020Quote: 96 well ELISA plates (Life Technologies) were coated with the anti-human HLA-E monoclonal capture antibody ...
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bioRxiv - Immunology 2020Quote: ... ELISA plates (Nunc™ Maxisorp™) were coated overnight with 100 μl of the relevant capture antibody at 4°C ...
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bioRxiv - Immunology 2022Quote: ... MaxiSorp high-binding ELISA plates (NUNC) were coated with 10μg/mL Spike protein in PBS (50mL per well) ...
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bioRxiv - Immunology 2022Quote: ... Mouse IFNg ELISA (Thermo Scientific, Cat.KMC4021) were performed according to the instruction ...
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bioRxiv - Microbiology 2022Quote: ... 1-step Ultra TMB-ELISA (ThermoFisher) was added to plates following manufacturer instructions ...
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bioRxiv - Microbiology 2022Quote: ... TMB-ELISA substrate solution (Thermo Fisher) was added and the reaction was stopped with 2N H2SO4 ...