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Citations for Thermo Fisher :
651 - 700 of 10000+ citations for Human Bcl2 Associated Death Promoter BAD ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... 293T cells were transfected with 1ug of a plasmid expressing the B/Florida/04/2006 NA under a CMV promoter using LipofectamineTM 3000 transfection reagent kit (Invitrogen). Cells were harvested and used for absorption after 48 hours of transfection.
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bioRxiv - Microbiology 2024Quote: All RNA was generated via in vitro transcription using a T7 RNA polymerase promoter and the MAXIscript T7 kit (ThermoFisher). SSR42 was amplified using OL7499/OL7500 while fragments of the lukA UTR and splE UTR were amplified using primers 7501/7502 and 7523/7524 ...
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bioRxiv - Immunology 2021Quote: Supernatants from MSC and T-cell cocultures were analyzed using IL-10 Ready SET-Go! ELISA kits kits (Affymetrix, eBioscience) for secretion of IL-10 as previously described [31] ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was then diluted 1:5 in 1x Assay buffer and processed according to the kit instructions (DNA Damage Competitive ELISA Kit, Invitrogen). The initial DNA concentration used in the assay was optimized to ensure the resulting values fall within the optimum range of kit detection limits (between Std3 and Std6) ...
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bioRxiv - Microbiology 2022Quote: ... ELISA signal was developed using 1-StepTM Ultra TMB-ELISA substrate solution (Thermo Scientific) and measured at an absorbance at 450 nm on a Perkin Elmer Wallac 1420 Victor2TM microplate reader or Cytation 5 cell imaging multi-mode reader (BioTek).
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bioRxiv - Microbiology 2022Quote: ... ELISA signal was developed using 1-StepTM Ultra TMB-ELISA substrate solution (Thermo Scientific) and measured at an absorbance of 450 nm on a Perkin Elmer Wallac 1420 Victor2TM microplate reader or Cytation 5 cell imaging multi-mode reader (BioTek).
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bioRxiv - Immunology 2021Quote: ... CD4+ and CD8+ T cells were isolated by negative selection with the Dynabeads Untouched Human CD4 T Cells Kit and the Dynabeads Untouched Human CD8 T Cells Kit (Invitrogen, Thermo Fisher Scientific) respectively ...
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bioRxiv - Molecular Biology 2023Quote: ... T cells were isolated from human PBMCs with an Untouched Human T-cell Isolation Kit (Invitrogen, USA) and activated with Dynabeads Human T Activator CD3/CD28 (Gibco ...
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bioRxiv - Neuroscience 2024Quote: ELISAs for human Aβ40 and human Aβ42 were carried out using commercial kits (Aβ40: Invitrogen KHB3481; Aβ42: Invitrogen KHB3441) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... Candidate promoters were cloned into pDONR-P4-P1R vector (Invitrogen) by BP recombination ...
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bioRxiv - Developmental Biology 2021Quote: ... cloned into the dual promoter pCRII-TA vector (ThermoFisher, #K207040) and used as template to generate DIG-labeled in situ probes with SP6/T7 polymerases (Roche ...
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bioRxiv - Developmental Biology 2023Quote: ... and TOPO-cloned into dual promoter empty vector (ThermoFisher, 450640). White colonies grown on LB plates with 100 mg/mL ampicillin and coated with X-Gal were selected and tested for an insert of correct size by restriction enzyme digest ...
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bioRxiv - Immunology 2022Quote: ... MaxiSporp ELISA plates (Thermo Fisher) were coated with respective antigen for 2h at RT and then blocked with 5% BSA (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2020Quote: ... 96-well ELISA plates (Nunc Maxisorp or Nunc Immobilizer Amino Plates ...
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bioRxiv - Microbiology 2022Quote: Nunc Maxisorp ELISA plates (ThermoFisher) were coated with 100 ng per well of SARS-CoV-2 B.1.1.529 (Omicron ...
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bioRxiv - Immunology 2020Quote: Nunc Maxisorp ELISA plates (ThermoFisher) were coated with 100 ng/well of protein in 1X PBS at 4°C for 16 hr ...
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bioRxiv - Immunology 2022Quote: ... 96-well ELISA plates (NUNC) were coated with 1 μg/mL recombinant SARS-CoV-2 spike RBD-His protein (Sino Biological 40592-V08B ...
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bioRxiv - Immunology 2020Quote: ... 96well ELISA plates (Nunc Maxisorp) were pre-treated with PBS/10% FCS (v/v ...
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bioRxiv - Microbiology 2020Quote: ... 96-well ELISA plates (Nunc) were coated overnight with 2.0 ug/mL (100ng/well ...
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bioRxiv - Microbiology 2020Quote: ... ELISA plates (Nunc, Thermo Scientific) were coated overnight with 5 µg protein diluted in PBSN3 and blocked with BSA10PBS for 2 hours at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... ELISA plates (Nunc, Thermo Scientific) were coated overnight with 5 µg protein diluted in PBSN3 and blocked with BSA10PBS for 2 hours at room temperature ...
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bioRxiv - Bioengineering 2021Quote: 96-well ELISA plates (Nunc MaxiSorp flat-bottom plates ...
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bioRxiv - Immunology 2021Quote: ... ELISA plates (NUNC, Thermo Fisher) were coated with 5 μg/ml γHV68 in coating buffer (0.05 M NaHCO3 ...
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bioRxiv - Immunology 2021Quote: ... ELISA plates (NUNC, Thermo Fisher) were coated with 5 μg/ml γHV68 in coating buffer (0.05 M NaHCO3 ...
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bioRxiv - Immunology 2020Quote: ... Nunc Maxisorp ELISA plates (ThermoFisher) were coated with 100 ng/well of stabilized soluble SARS-CoV-2 spike protein24 (with His-tag cleaved to remove potential cross-reactivity ...
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bioRxiv - Immunology 2023Quote: ... ELISA plates (96-well, Nunc) were coated overnight at 4°C with 1 µg/mL purified S1+S2 antigen (Sino Biological ...
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bioRxiv - Bioengineering 2023Quote: ... An EGF ELISA (Invitrogen, EREGF) was performed on the collected basal supernatant following supplier instructions with samples ran in duplicate and averaged ...
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bioRxiv - Immunology 2024Quote: ... or IFNγ by ELISA (Invitrogen) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2023Quote: ... 96-well ELISA plate (GIBCO) by rocking for 1h at RT ...
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bioRxiv - Cell Biology 2022Quote: ... Mouse Spp1(OPN) ELISA (Invitrogen) assays were performed according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ELISA plates (ThermoFisher 269620 439454) were precoated with 100 μL PBS containing 2ug/mL LAM overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... and ELISA (Invitrogen, ThermoFisher Scientific) assays on cell supernatants were performed according to the manufacter recommendations ...
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bioRxiv - Immunology 2024Quote: ... and ELISA (Invitrogen, ThermoFisher Scientific) assays on cell supernatants were performed according to the manufacter recommendations ...
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bioRxiv - Biochemistry 2024Quote: ... Nunc Maxisorp ELISA plates (Nunc) were coated overnight with purified anti-IL2 antibody (clone JES6-1A12 ...
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bioRxiv - Immunology 2024Quote: ... 96-well ELISA plates (ThermoFisher) were coated with 100 ng/well of peanut extract protein (Ukko) ...
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bioRxiv - Cancer Biology 2021Quote: ... p53-associated protein were pulled down by 5 μl DynabeadsTM protein G beads (#10004D, Invitrogen) at 4 °C for 1-1.5 h ...
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bioRxiv - Molecular Biology 2021Quote: ... and raw data analysis carried out using the associated Ion Torrent Suite 5.0.2 (ThermoFisher Scientific). Ion PGM template OT2 kits (4480974 ...
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bioRxiv - Molecular Biology 2023Quote: ... or ALS-associated Ruby-tagged sequences of DnaJC7 using 5 μL of Lipofectamine 2000 (Gibco) transfection reagent in OptiMEM (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... biofilm matrix-associated sugar content using fluorescein wheat germ agglutinin (WGA, 1/100 diluted, ThermoFisher), and viability using a resazurin assay (1 mg/ml ...
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bioRxiv - Immunology 2021Quote: ... Live/death staining was performed with fixable eFluor506 viability dye (1:2000, 65-0866-14, ThermoFisher) for 30 min at RT in PBS ...
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bioRxiv - Cell Biology 2021Quote: ... cell death was assessed using SYTOX Green™ nucleic acid stain at 250nM concentration (ThermoFisher Scientific) plus the addition of several cell death inducers ...
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bioRxiv - Bioengineering 2023Quote: ... and changes in cell death in the tumoroids assessed through imaging of SYTOX Green (#S34860, ThermoFisher) on a Nikon Eclipse Ti microscope with a 4X objective ...
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bioRxiv - Immunology 2023Quote: ... Cells were stained for cell death using either LIVE/DEAD Fixable Aqua Dead Cell marker (Invitrogen) or GhostDye Violet 510 Viability Dye (TONBO Biosciences ...
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bioRxiv - Cancer Biology 2022Quote: ... 300 cells were analyzed for cell death using a 0.4% trypan blue solution (Thermo Fisher Scientific) 24 hours after stress induction ...
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bioRxiv - Immunology 2023Quote: Neutrophils cell death was detected and quantified using the annexin V dye (Thermo Fisher Scientific, A23202). Isolated cells were seeded and incubated for 30 min at 37°C followed by centrifugation to form a monolayer ...
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bioRxiv - Systems Biology 2024Quote: ... Colons were collected within 5 min of death and flushed using ice-cold 1x PBS (Gibco) using a straight gauge needle with a 2.4mm tip (Kent Scientific Corporation ...
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bioRxiv - Synthetic Biology 2024Quote: ... the genes were subsequently cloned into binary vectors pMDC32 (2×35S promoter)67 or pGWB2 (35S promoter)68 using LR clonase II (Thermo Fisher Scientific, Waltham, MA, USA) following the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2021Quote: ... Cas9 mRNA for mouse zygote injection was in-vitro transcribed from the XbaI-linearized Cas9 plasmid with a T7 promoter using mMESSAGE mMACHINE T7 Ultra Kit (Life Technologies). The sgRNAs were in-vitro transcribed from PCR products with the MEGAshortscript Kit (Life Technologies) ...
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bioRxiv - Microbiology 2020Quote: ... the non-structural gene 4 (NS4) was amplified with primers containing the T7 promoter sequence followed by reverse transcription with MEGAscript T7 Transcription Kit (Thermo Fisher) to generate AEFV NS4 RNA ...
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bioRxiv - Cancer Biology 2021Quote: ... A T7 RNA polymerase promoter sequence was added to allow for in vitro transcription using the MEGAscript T7 transcription kit (Thermo Fisher). RNA was end labelled at the 3’ end with biotin using the Pierce RNA 3’ End Biotinylation Kit (Thermo Fisher) ...