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Citations for Thermo Fisher :
751 - 800 of 10000+ citations for Glutathione Reductase Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... The nuclei were stained by incubating the cells with PBS containing fluorescent secondary antibodies (1:1,000, A21207, Invitrogen) and DRAQ5™ (1:1,000 ...
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bioRxiv - Microbiology 2022Quote: ... Host appropriate secondary antibodies were labeled with a red fluorescent dye Alexa Flour 594 (Life Technologies; 1:300) or biotinylated secondary antibody (Vector Laboratories ...
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bioRxiv - Systems Biology 2023Quote: RNA was reverse-transcribed from annealed fluorescent primer in a reaction containing 1× First Strand Buffer (Thermo Fisher), 5 mM DTT ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then washed with PBS and incubated in Alexa Fluor-conjugated fluorescent secondary antibodies (Invitrogen; 1:500) for 2 hr at room temperature ...
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bioRxiv - Physiology 2022Quote: ... then incubated for 2 hours at room temperature in Alexa Fluor fluorescent secondary antibody (Life Technologies; 1:500) prepared in blocking solution ...
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bioRxiv - Neuroscience 2024Quote: ... samples were incubated in fluorescent secondary antibody solutions: goat anti-mouse Alexa Fluor 488 (1:500; Life Technologies) and goat anti-rabbit Alexa Flour 555 (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... followed by incubation with a 1:20 000 dilution of a fluorescent-dye-coupled secondary antibody (Thermofisher Scientific) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... followed by overnight incubation with fluorescent secondary antibodies (Fluor 568 labeled donkey anti-Mouse (1:500; Life Technologies), Fluor 488 labeled donkey anti-rabbit (1:500 ...
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bioRxiv - Biophysics 2024Quote: Larval muscles were stained with fluorescent nanobodies (Loreau et al., 2023) and rhodamine-phalloidin (1:1000 Molecular Probes) for 2 hr at RT or overnight at 4 °C as previously described in detail (Loreau et al. ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were incubated with a secondary antibody solution containing fluorescent-labeled anti-mouse antibody (Thermo Fisher, 1:200), Streptavidin (Thermo Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... washed twice with PBS and incubated with appropriate Alexa Fluor-conjugated fluorescent secondary antibodies (1:400, Life Technologies) for an additional hour ...
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bioRxiv - Immunology 2024Quote: ... 100 nM) were loaded on 2 % agarose gel containing 1 × fluorescent dye SYBR Safe DNA Gel Stain (Invitrogen), run at 150 V for 45 minutes ...
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bioRxiv - Cell Biology 2022Quote: The concentration of secreted Tgfβ1 ligand levels was measured by taking 3-day conditioned media at d5 of culture with Tgf beta-1/LAP Rat Uncoated ELISA Kit with Plates by following the manufacturer’s protocol (ThermoFisher Scientific, Waltham, MA). The cells were cultured in a 2% FBS media for ELISA ...
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bioRxiv - Synthetic Biology 2022Quote: ... Selective plates contained 15 g L-1 Noble agar (Affymetrix) to eliminate bacterial growth on carbon source contaminants available in the agar ...
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bioRxiv - Immunology 2020Quote: ... Plates were developed with 1-step Ultra TMB-ELISA (ThermoFisher), reaction stopped by 2M H2SO4 and read at 450nm.
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bioRxiv - Biochemistry 2019Quote: ... 1 mM DTT using Zeba spin desalting plates (Thermo Scientific) according to the manufacturer’s instructions ...
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Targeting Noradrenergic Neurons of the Locus Coeruleus: A Comparison of Model Systems and StrategiesbioRxiv - Neuroscience 2022Quote: ... containing primary antibodies (rabbit against tyrosine hydroxylase, 1:1000, AB152, Merck Millipore; and chicken against green-fluorescent protein, 1:750, A10262, Invitrogen, Thermo Fisher Scientific) to allow for sufficient target protein-primary antibody interaction ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were then rinsed in TBS and stained with a fluorescent Nissl (1:500, 1 hr; NeuroTrace™; N21479, 435/455nm, Thermo Fisher Scientific). Slides were then coverslipped with Vectashield hardset antifade mounting medium with a DAPI counterstain (H-1500-10 ...
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bioRxiv - Plant Biology 2022Quote: ... Leaves from T0 regenerants bearing a fluorescent reporter were stained with Propidium Iodide (PI) (1:100 dilution of 1 mg/ml stock Molecular Probes; Invitrogen detection technologies) and visually inspected using confocal microscopy for expression of the reporter as confirmation of successful transformation ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated for 2 h at RT with appropriate fluorescent secondary antibodies diluted in PBS – 1% NGS: goat anti- rabbit Alexa Fluor® 488 (1/1000, Invitrogen, A11008, RRID:AB_143165) or goat anti-rabbit Alexa Fluor® 568 (1/1000 ...
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bioRxiv - Neuroscience 2022Quote: ... Live cultures or thawed cells were either expanded as floor plate progenitors (following Brundin approach; Floor Plate Progenitor Kit expansion protocol (ThermoFisher; A3165801) or terminally differentiated to DA neurons with all prior factors except in neurobasal base medium after day 25.
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bioRxiv - Immunology 2023Quote: ... A 10 μl supernatant sample was mixed with 90 μl substrate solution containing 0.5 mg/ml of 4-methylumbelliferyl N,N’-diacetyl-β-D -chitobioside in an assay buffer provided as part of the kit and in a 96-well plate (black/clear bottom plate, Thermo Fisher). The reaction was incubated at 37 °C for 30 min and then stopped by adding 100 μl stop solution ...
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bioRxiv - Neuroscience 2020Quote: ... To produce a suspension of fluorescent microspheres adequate for this purpose 1.2μl of stock solution of 0.5μm fluorescent microspheres (Invitrogen Tetraspeck 0.5μm – Cat No. T7281) was added to 100μl of molten agarose (1.2% w/v ...
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bioRxiv - Bioengineering 2019Quote: Fluorescent beads: In this study we used Yellow/Green fluorescent nanoparticles with a diameter of 100 nm (FluoSpheres, ThermoFisher Scientific, USA).
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bioRxiv - Developmental Biology 2022Quote: Fluorescent in situ hybridization was performed according to standard protocols adding a secondary fluorescent antibody (anti-goat Alexa555, Invitrogen A-21432); cv-c riboprobe was marked using DIG RNA Labelling Kit (Roche ...
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bioRxiv - Neuroscience 2024Quote: ... The fluorescent protein expressed by the virus (either GFP or mCherry) was visualized using an EVOS FL tabletop fluorescent microscope (ThermoFisher Scientific). Images were taken for future reference ...
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bioRxiv - Cell Biology 2021Quote: ... GST-Arl15 immobilized on glutathione Sepharose beads was incubated with 50 mM dimethyl pimelimidate (Thermo Fisher Scientific) in 200 mM sodium borate pH 9.0 to crosslink GST-Arl15 covalently onto glutathione Sepharose beads ...
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bioRxiv - Plant Biology 2020Quote: ... The GST and GST-XIK fusion proteins were extracted with Pierce Immobilized Glutathione resin (Thermo Fisher Scientific) with the protein interaction buffer (20 mM Hepes-KOH pH7.2 ...
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bioRxiv - Cell Biology 2019Quote: ... GST or GST-EB1 (5 μg) were incubated with 20 μl glutathione agarose beads (16100; Thermo Scientific) for 2 h at 4°C ...
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bioRxiv - Immunology 2023Quote: Intracellular reduced glutathione levels were determined using commercially available fluorometric thiol probe thiol tracker Violet (Thermo Fisher) and the total glutathione (GSH ...
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bioRxiv - Immunology 2023Quote: ... The supernatant fraction containing the GST-tagged constructs were loaded onto Protino Glutathione Agarose 4B (Fisher Scientific) beads and impurities were removed by washing with TBS ...
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bioRxiv - Cell Biology 2023Quote: ... Crude lysates were then clarified by centrifugation and the supernatant was incubated with glutathione magnetic beads (ThermoFisher), conjugated with the Rhotekin Rho Binding domain (RBD)-GST fusion protein (produced from Addgene plasmid #15247) ...
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bioRxiv - Molecular Biology 2023Quote: ... 1:200 dilution of primary culture was added to sterile 96-well plate black/clear bottom plates (Thermo Scientific) in a final volume of 200 µL with 0.1 mM IPTG and antibiotics ...
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bioRxiv - Bioengineering 2021Quote: ... the amount of double stranded DNA measured in the supernatants using a fluorescent Quant-iT Picogreen dsDNA Assay kit (Thermo Fisher), and the amount of ALP measured using SigmaFast™ p-Nitrophenyl phosphate (pNPP ...
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bioRxiv - Immunology 2022Quote: ... and subsequently stained for 1 h at room temperature with anti-NP mAb 1C7 antibody labeled with an Alexa488-fluorescent marker using the Alexa Fluor™ 488 Antibody Labeling Kit (Invitrogen). Immunofluorescence-labelled cells were washed twice in PBS-EDTA after incubation with the antibody and then subjected to flow-cytometry analysis using a Gallios flow cytometer (Beckman Coulter) ...
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bioRxiv - Genomics 2019Quote: ... Final DNA concentration was measured using a dsDNA-specific fluorescent dye (Quant-iT HS dsDNA Assay Kit, ThermoFisher, Waltham, MA, USA).
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bioRxiv - Cancer Biology 2020Quote: ... for 2 hours and fluorescent labeling was performed with the Click-IT EdU Alexa Fluor 488 Flow Cytometry Assay kit (Thermo Fisher), according to the manufacturer’s description ...
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bioRxiv - Neuroscience 2021Quote: ... Purified small RNA sequencing libraries were quantified using the fluorescent dye-based Qubit ds DNA HS Assay Kit (Thermo Fisher Scientific) on a 2100 Bioanalyzer ...
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bioRxiv - Cell Biology 2021Quote: ... The microscope was adjusted and checked for the absence of chromatic shift during fluorochrome detection from different spectral regions using the TetraSpeck Fluorescent Microspheres Size Kit (ThermoFisher Scientific). Sequential scanning mode was used for each of the four channels (DAPI ...
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bioRxiv - Developmental Biology 2019Quote: ... cydippids were fixed and processed for fluorescent detection of incorporated EdU using the Click-iT EdU labeling kit (Invitrogen Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were washed twice with PBS and incorporated EU was detected by click chemistry using a fluorescent azide following the manufacturer’s guidelines of Click-iT RNA Imaging Kit (Thermo Fisher Scientific). Following the Click-iT reaction (30 min at RT in the dark) ...
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bioRxiv - Microbiology 2023Quote: ... the instrument was set using the Flow Cytometry Sub-micron Particle Size Reference Kit providing green-fluorescent beads of different sizes (Invitrogen™) to establish the correct threshold value to apply on the 525/40 nm fluorescent channel (FL1) ...
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bioRxiv - Microbiology 2023Quote: ... according to manufacturer’s instructions and quantified using the fluorescent dye-based Qubit® dsDNA HS Assay Kit (Invitrogen, Carlsbad, California, USA). Samples were equimolarly pooled and sequenced using the Illumina MiSeq platform (Illumina Inc. ...
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bioRxiv - Plant Biology 2022Quote: ... Leaves from T0 regenerants bearing a fluorescent reporter were stained with Propidium Iodide (PI) (1:100 dilution of 1 mg/ml stock Molecular Probes; Invitrogen detection technologies) and visually inspected using confocal microscopy for expression of the reporter as confirmation of successful transformation ...
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bioRxiv - Cell Biology 2022Quote: ... and 1 mM ATP) and then stained with a far-red fluorescent membrane dye (cat no. C10046; Thermo Fisher). Prior to the cell stiffness assay ...
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bioRxiv - Cell Biology 2020Quote: ... pH 7.1) and fluorescent labelling was performed by incubating the beads with 1 μM Alexa-568 C5 Maleimide (A20341, ThermoFisher). Unreacted dye was removed with 10 ml CSF-XBg buffer ...
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bioRxiv - Biochemistry 2019Quote: ... fluorescent probes Prodan (6-propionyl-2[dimethylamino]-naphthalene) and ANS (1-anilinonaphthalene-8-sulfonic acid) were also from Invitrogen; PMB ...
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bioRxiv - Microbiology 2019Quote: ... cultures were supplemented with fluorescent 1 ug/ml Wheat Germ Agglutinin conjugated to Alexa Fluor™ 594 (Thermo Fisher) (fWGA ...
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bioRxiv - Neuroscience 2019Quote: ... Thiol reactive fluorescent probe IAEDANs (1,5-IAEDANS, 5-((((2-iodoacetyl)amino)ethyl)amino)naphthalene-1-sulfonic Acid) was purchased from ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... sections were washed three times in TBS followed by incubation for 2 hours at room temperature in TBS with 2% normal donkey serum and 0.3% triton X-100 containing the fluorescent secondary antibody (Donkey anti rabbit Alexa-488, 1:300, A-21206, Invitrogen) and finally by the nuclear counter stain DAPI (1:1000 ...