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Citations for Thermo Fisher :
701 - 750 of 10000+ citations for Glutathione Reductase Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... More precise and accurate quantification was performed with sensitive fluorescent-based quantitation assays (“Quant-It” assays kit and QuBit fluorometer, Invitrogen). Samples were normalized at 2 nM concentrations ...
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bioRxiv - Microbiology 2021Quote: ... Cell monolayers were then loaded with the fluorescent substrate by adding 20 µl of 6X CCF4/AM solution (LiveBLAzer-FRET B/G Loading Kit, Invitrogen) containing 15 mM Probenecid (Sigma) ...
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bioRxiv - Neuroscience 2020Quote: ... Total RNA was quantified using fluorescent chemistry contained in the Qubit RNA HS Assay Kit (Cat# Q10211, Thermo Fisher Scientific). 1 ul was used to assess RNA Integrity Number (RIN ...
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bioRxiv - Microbiology 2019Quote: ... more precise and accurate quantification is performed with sensitive fluorescent-based quantitation assays (“Quant-It” assays kit and QuBit fluorometer, Invitrogen).
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bioRxiv - Microbiology 2022Quote: ... coli cells in the single-cell microscopy experiments we used Live/dead® BacLight™ fluorescent stain kit from Invitrogen, California ...
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bioRxiv - Neuroscience 2023Quote: ... and loaded with the Fluorescent Dye-Based Rhod-3 AM following the manufactures’ instructions (Rhod-3 Calcium Imaging Kit, Cat.No. R10145; ThermoFisher scientific). Loading ...
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bioRxiv - Microbiology 2023Quote: ... Cell monolayers were then loaded with the fluorescent substrate by adding 20 μl of 6X CCF4/AM solution (LiveBLAzer-FRET B/G Loading Kit, Invitrogen) containing 15 mM Probenecid (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... More precise and accurate concentration estimates were obtained with a fluorescent-based quantitation assay (QuBit fluorometer, “Quant-It” assays kit, Invitrogen). Concentrations were normalized to 2 nM ...
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bioRxiv - Biochemistry 2023Quote: ... The degree of labeling of the proteins with the fluorescent probes was evaluated by following the manufacturer’s protocol (Microscale Protein Labeling Kit, ThermoFisher Scientific).
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bioRxiv - Molecular Biology 2023Quote: ... Sequencing reactions were performed using a commercial kit designed to be used with fluorescent dye-labeled primers (Thermo Fisher Scientific). ExoSAP-IT reagent (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: The concentration of secreted Tgfβ1 ligand levels was measured by taking 3-day conditioned media at d5 of culture with Tgf beta-1/LAP Rat Uncoated ELISA Kit with Plates by following the manufacturer’s protocol (ThermoFisher Scientific, Waltham, MA). The cells were cultured in a 2% FBS media for ELISA ...
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bioRxiv - Synthetic Biology 2022Quote: ... Selective plates contained 15 g L-1 Noble agar (Affymetrix) to eliminate bacterial growth on carbon source contaminants available in the agar ...
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bioRxiv - Immunology 2020Quote: ... Plates were developed with 1-step Ultra TMB-ELISA (ThermoFisher), reaction stopped by 2M H2SO4 and read at 450nm.
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bioRxiv - Biochemistry 2019Quote: ... 1 mM DTT using Zeba spin desalting plates (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2021Quote: ... slices were incubated with fluorescently-labeled secondary antibodies at 1:500 and NeuroTrace Blue Fluorescent Nissl Stain (Invitrogen) at 1:250 in MAXStain Staining medium overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated with the fluorescent secondary antibodies (Alexa Fluor 568-conjugated anti-rabbit IgG, 1:500, Invitrogen, A11011 ...
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bioRxiv - Neuroscience 2021Quote: ... were applied with the added inclusion of fluorescent-conjugated streptavidin (Streptavidin AlexaFluor 633, 1:500, Invitrogen, Dunfermline, UK) to visualise recorded neurons.
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bioRxiv - Biophysics 2019Quote: ... The gels contained a thin top layer (10 μm) impregnated with 1 μm fluorescent beads (FluoSperes; Molecular Probes) that acted as fiduciary markers of substrate deformation ...
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bioRxiv - Bioengineering 2021Quote: ... the cultures were exposed to appropriate fluorescent secondary antibodies (1/500, ThermoFisher, Alexa-488, Alexa-568, Alexa-647) in 4% horse serum for 2 hr at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... VSMCs were seeded onto 12 kPa hydrogels containing 0.5 µm red fluorescent (580/605) FluoSpheres (1:1000) (Invitrogen). Imaging was performed using a Zeiss Axio Observer live cell imaging system that captured 20x magnification images every 2 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... Lipid mixes contained a small amount (1 mol%) of the fluorescent lipid probe pTexas-Red DHPE (Thermo Fisher). 2 μl of the lipid mix was spread with a glass syringe on a PEGylated glass coverslip ...
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bioRxiv - Neuroscience 2020Quote: ... cells were incubated with a fluorescent secondary antibody (donkey anti-rabbit Alexa Fluor 488-conjugated, Invitrogen, 1/500) in 1% donkey serum ...
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bioRxiv - Neuroscience 2021Quote: ... followed by fluorescent-conjugated secondary antibodies and counterstained with Hoechst 33342 (ThermoFisher 62249, 1 μg/mL in PBS). F-actin was labelled prior to cell fixation using Phalloidin-647 (Abcam ab176759) ...
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bioRxiv - Biophysics 2022Quote: ... Rather than purified sensor,20 μl of a 1/10,000 dilution of carboxylate latex fluorescent beads (Fluospheres, Invitrogen) of either 20 nm (F-8786) ...
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bioRxiv - Microbiology 2020Quote: Phagocytosis assays were performed in vivo as previously described 8,32 using red fluorescent FluoSpheres (1 μm Molecular Probes) at a 1:10 dilution in 1x PBS ...
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bioRxiv - Biophysics 2022Quote: ... The nuclei were stained by incubating the cells with PBS containing fluorescent secondary antibodies (1:1,000, A21207, Invitrogen) and DRAQ5™ (1:1,000 ...
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bioRxiv - Microbiology 2022Quote: ... Host appropriate secondary antibodies were labeled with a red fluorescent dye Alexa Flour 594 (Life Technologies; 1:300) or biotinylated secondary antibody (Vector Laboratories ...
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bioRxiv - Systems Biology 2023Quote: RNA was reverse-transcribed from annealed fluorescent primer in a reaction containing 1× First Strand Buffer (Thermo Fisher), 5 mM DTT ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then washed with PBS and incubated in Alexa Fluor-conjugated fluorescent secondary antibodies (Invitrogen; 1:500) for 2 hr at room temperature ...
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bioRxiv - Physiology 2022Quote: ... then incubated for 2 hours at room temperature in Alexa Fluor fluorescent secondary antibody (Life Technologies; 1:500) prepared in blocking solution ...
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bioRxiv - Cell Biology 2023Quote: ... followed by incubation with a 1:20 000 dilution of a fluorescent-dye-coupled secondary antibody (Thermofisher Scientific) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... followed by overnight incubation with fluorescent secondary antibodies (Fluor 568 labeled donkey anti-Mouse (1:500; Life Technologies), Fluor 488 labeled donkey anti-rabbit (1:500 ...
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bioRxiv - Biophysics 2024Quote: Larval muscles were stained with fluorescent nanobodies (Loreau et al., 2023) and rhodamine-phalloidin (1:1000 Molecular Probes) for 2 hr at RT or overnight at 4 °C as previously described in detail (Loreau et al. ...
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bioRxiv - Neuroscience 2024Quote: ... samples were incubated in fluorescent secondary antibody solutions: goat anti-mouse Alexa Fluor 488 (1:500; Life Technologies) and goat anti-rabbit Alexa Flour 555 (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... Live cultures or thawed cells were either expanded as floor plate progenitors (following Brundin approach; Floor Plate Progenitor Kit expansion protocol (ThermoFisher; A3165801) or terminally differentiated to DA neurons with all prior factors except in neurobasal base medium after day 25.
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bioRxiv - Immunology 2023Quote: ... A 10 μl supernatant sample was mixed with 90 μl substrate solution containing 0.5 mg/ml of 4-methylumbelliferyl N,N’-diacetyl-β-D -chitobioside in an assay buffer provided as part of the kit and in a 96-well plate (black/clear bottom plate, Thermo Fisher). The reaction was incubated at 37 °C for 30 min and then stopped by adding 100 μl stop solution ...
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Targeting Noradrenergic Neurons of the Locus Coeruleus: A Comparison of Model Systems and StrategiesbioRxiv - Neuroscience 2022Quote: ... containing primary antibodies (rabbit against tyrosine hydroxylase, 1:1000, AB152, Merck Millipore; and chicken against green-fluorescent protein, 1:750, A10262, Invitrogen, Thermo Fisher Scientific) to allow for sufficient target protein-primary antibody interaction ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were then rinsed in TBS and stained with a fluorescent Nissl (1:500, 1 hr; NeuroTrace™; N21479, 435/455nm, Thermo Fisher Scientific). Slides were then coverslipped with Vectashield hardset antifade mounting medium with a DAPI counterstain (H-1500-10 ...
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bioRxiv - Plant Biology 2022Quote: ... Leaves from T0 regenerants bearing a fluorescent reporter were stained with Propidium Iodide (PI) (1:100 dilution of 1 mg/ml stock Molecular Probes; Invitrogen detection technologies) and visually inspected using confocal microscopy for expression of the reporter as confirmation of successful transformation ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated for 2 h at RT with appropriate fluorescent secondary antibodies diluted in PBS – 1% NGS: goat anti- rabbit Alexa Fluor® 488 (1/1000, Invitrogen, A11008, RRID:AB_143165) or goat anti-rabbit Alexa Fluor® 568 (1/1000 ...
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bioRxiv - Neuroscience 2020Quote: ... To produce a suspension of fluorescent microspheres adequate for this purpose 1.2μl of stock solution of 0.5μm fluorescent microspheres (Invitrogen Tetraspeck 0.5μm – Cat No. T7281) was added to 100μl of molten agarose (1.2% w/v ...
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bioRxiv - Bioengineering 2019Quote: Fluorescent beads: In this study we used Yellow/Green fluorescent nanoparticles with a diameter of 100 nm (FluoSpheres, ThermoFisher Scientific, USA).
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bioRxiv - Developmental Biology 2022Quote: Fluorescent in situ hybridization was performed according to standard protocols adding a secondary fluorescent antibody (anti-goat Alexa555, Invitrogen A-21432); cv-c riboprobe was marked using DIG RNA Labelling Kit (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... 1:200 dilution of primary culture was added to sterile 96-well plate black/clear bottom plates (Thermo Scientific) in a final volume of 200 µL with 0.1 mM IPTG and antibiotics ...
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bioRxiv - Cell Biology 2021Quote: ... GST-Arl15 immobilized on glutathione Sepharose beads was incubated with 50 mM dimethyl pimelimidate (Thermo Fisher Scientific) in 200 mM sodium borate pH 9.0 to crosslink GST-Arl15 covalently onto glutathione Sepharose beads ...
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bioRxiv - Plant Biology 2020Quote: ... The GST and GST-XIK fusion proteins were extracted with Pierce Immobilized Glutathione resin (Thermo Fisher Scientific) with the protein interaction buffer (20 mM Hepes-KOH pH7.2 ...
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bioRxiv - Cell Biology 2019Quote: ... GST or GST-EB1 (5 μg) were incubated with 20 μl glutathione agarose beads (16100; Thermo Scientific) for 2 h at 4°C ...
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bioRxiv - Immunology 2023Quote: Intracellular reduced glutathione levels were determined using commercially available fluorometric thiol probe thiol tracker Violet (Thermo Fisher) and the total glutathione (GSH ...
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bioRxiv - Immunology 2023Quote: ... The supernatant fraction containing the GST-tagged constructs were loaded onto Protino Glutathione Agarose 4B (Fisher Scientific) beads and impurities were removed by washing with TBS ...
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bioRxiv - Cell Biology 2023Quote: ... Crude lysates were then clarified by centrifugation and the supernatant was incubated with glutathione magnetic beads (ThermoFisher), conjugated with the Rhotekin Rho Binding domain (RBD)-GST fusion protein (produced from Addgene plasmid #15247) ...