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Citations for Thermo Fisher :
751 - 800 of 10000+ citations for 7H Diimidazo 1 5 a 1 5 4 de quinoxaline 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... samples were incubated with a 5 μM DRAQ-5 (Thermo Scientific) DNA staining buffer at 37° C for 30 minutes with agitation ...
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bioRxiv - Biochemistry 2024Quote: hSSB2 was labeled with 5-IAF (5-iodoacetamido-fluorescein, Thermo Fisher) on the intrinsic cysteines ...
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bioRxiv - Genomics 2023Quote: ... and their media was replaced on day 1 with DE induction Media A (Gibco Cat# A3062601), and on day 2 with DE induction Media B (Gibco Cat# A3062601) ...
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bioRxiv - Molecular Biology 2019Quote: ... To initiate transcription 4 µL of 5 X first strand buffer (Invitrogen, Cat No. 1889832) and 1 µL each of 0.1 M DTT (Invitrogen ...
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Potassium channel-driven bioelectric signaling regulates metastasis in triple-negative breast cancerbioRxiv - Cancer Biology 2021Quote: ... CDH11 #4: 5’-CCUUAUGACUCCAUUCAAA-3’ using Lipofectamine RNAiMAX transfection reagent (13778030; ThermoFisher Scientific, Waltham, MA) in serum-free DMEM ...
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bioRxiv - Microbiology 2022Quote: ... A 4’-5-diamidina-2-phenylindole (DAPI)-containing mounting medium was used (ThermoFisher, cat. 62248). Images were acquired using a spectral Zeiss LSM800 confocal microscope or Confocal Zeiss LSM980 - AiryScan and analyzed with Fiji software (version 2.0.0) ...
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bioRxiv - Cell Biology 2021Quote: ... A 4’-5-diamidina-2-phenylindole (DAPI)-containing mounting medium was used (ThermoFisher, cat. 62248). Images were acquired using a spectral Zeiss LSM800 confocal microscope and analyzed with Fiji software (version 2.0.0) ...
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bioRxiv - Biochemistry 2021Quote: ... then fixed with 4% paraformaldehyde containing 5 µg/ml Hoechst 33342 stain (Thermo Fisher, #62249) for 10 min at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... 5-7 μg BACMID DNA was incubated with 4 μL Fugene (Thermo Fisher, Cat# 10362100) in 200 μL of ESF 921 media for 30 minutes at 23°C ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated overnight (4° C) with 5 µg of Cx36 (Thermofisher, 37-4600, RRID: AB_2533320) or NMDAR1 (Thermofisher ...
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bioRxiv - Microbiology 2020Quote: ... 5-10 ul of each sample was loaded on 4-12% Bis-Tris gels (Thermofisher).
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bioRxiv - Microbiology 2021Quote: ... and 4 mg/l 5-(and-6)-carboxyfluorescein and succinimidyl ester (FITC; Thermo Fisher Scientific), respectively ...
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Activation of innate immune cGAS-STING pathway contributes to Alzheimer’s pathogenesis in 5×FAD micebioRxiv - Neuroscience 2022Quote: ... Slides were counterstained with 5 μg/mL 4’,6-diamidino-2-phenylindole (DAPI; ThermoFisher Scientific) for 10 min at room temperature and washed with 1 × PBST (0.2% Triton-X 100 ...
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bioRxiv - Neuroscience 2022Quote: ... for 5 min and loaded onto 4%–12% Nupage Bis-Tris gels (Novex/Life Technologies). Proteins were transferred using the iBlot2 (Life Technologies ...
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bioRxiv - Microbiology 2022Quote: ... 4-5 ×106 P25 TCR-Tg CD4+ T cells (CD45.1) were labeled with CellTrace (Invitrogen) and adoptively transferred by tail vein injection in 100 µL of sterile PBS 24 hours (h ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were passaged every 4-5 days by treatment with StemPro Accutase (ThermoFisher, Cat#A1110501), stored in liquid nitrogen ...
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bioRxiv - Microbiology 2023Quote: ... the 11.5 μL RNA were supplemented with 4 μL 5× first strand buffer (ThermoFisher Scientific), 1 μL 10 μM reverse primer ...
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bioRxiv - Cancer Biology 2023Quote: ... for 5 min Samples were run down a Novex 4–12% Tris-glycine gel (Invitrogen) and transferred onto a nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... and cells were split every 4–5 days using 0.5 mM EDTA (Invitrogen, 15575-020). hiPS cell clones/lines were also generated using retroviruses or non-integrating episomal vectors from fibroblasts (Bhaduri et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... PDCA-1 (ThermoFisher # 53-3179-4), TLR9 (Biolegend #394803) ...
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bioRxiv - Pathology 2023Quote: ... Fluo-4-AM (1 µM; Invitrogen), in the dark for 20 minutes at room temperature in Mg2+-PSS ...
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bioRxiv - Neuroscience 2023Quote: ... (1:300; 4 h, RT, Invitrogen) were used as secondary antibodies ...
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bioRxiv - Neuroscience 2020Quote: ... blocked for 1 hour in 5 % normal donkey serum in 3% PBST and then incubated in chicken anti-GFP (Invitrogen, A10262, 1:1000) overnight at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... sections were rinsed 5 x 5 mins and 1 x 1hr before corresponding secondary antibodies along with the nuclear stain Hoechst33342 (1:5000; Molecular Probes; H-3570) were incubated overnight at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were placed in a thermocycler and when the temperature reached 55°C, 10 µL of enzyme mix (1 µL 10X ThermoPol Reaction buffer, 1 µL (5 units) Taq DNA polymerase (Thermo Fisher Scientific, 18038240) and 0.01 µL (0.025 units ...
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bioRxiv - Genetics 2019Quote: ... Membranes were incubated for 1 hour at room temperature in 1xTBST + 5% non-fat milk with primary antibodies (1:2000 α-Myc 9E10, 1:5000; α-Vma2 (Life Technologies); 1:5000 α-Sir2 (Santa Cruz Biotechnology) ...
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bioRxiv - Cell Biology 2020Quote: ... MCF10A were cultured in [5 mM] glucose DMEM:F12 (1:1 mixture of F:12 [10mM] glucose and [0 mM] glucose DMEM) (Life Technologies, 11765054 and 11966025) supplemented with 5% Horse Serum (Gibco ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then washed three times for 5 min at RT in TNT buffer and incubated with 0.5 µg ml−1 DAPI and Alexa555-Streptavidin (Thermo Fisher, #32355, 1:1,000) at room temperature for 1 h ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... centrifuged for 5’ on 1.4 rpm and were incubated with Fc Blocker (1:100, in 1XPBS + 1%FBS, Thermo Fisher Scientific, Ghent, Belgium) for 30’ on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were washed by PBS with 0.1% Tween 20 (PBST) (three times for 5 min each) and incubated with Alexa Fluor secondary antibodies 1:500 (Thermo Fisher, #A-11036) for 1 hour ...
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bioRxiv - Bioengineering 2023Quote: ... pRSV-Rev = 5: 3: 2) were co-transfected into HEK-293T cells at a ratio of 1:1 using lipofectamine 3000 (Invitrogen, USA, Cat: #L3000015). After 48 h ...
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bioRxiv - Neuroscience 2023Quote: ... An imaging window was constructed from three layers of microscope cover glass (1 x 5 mm, 2 x 3 mm diameter, Fisher Scientific, no. 1) joined with a UV-curable optical glue (NOR-61 ...
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bioRxiv - Neuroscience 2024Quote: ... Germany) were grown at 37°C and 5% CO2 in a 1:1 mixture of Opti-MEM + Glutamax (Thermo Fisher Scientific; 51985-026) and DMEM (Dulbecco’s Modified Eagle Medium ...
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bioRxiv - Bioengineering 2023Quote: ... 4 µM ethidium homodimer-1 and 1 µg ml-1 Hoechst 33342 (Thermofisher, 62249) was added to chondrocyte-laden alginate hydrogels and was incubated for one hour ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were centrifuged at 14,000g for 5 minutes at 4°C and electrophoresed on NuPAGE™ 4-12% Bis-Tris Polyacrylamide gels (Thermo Fisher) with NuPAGE™ MOPS running buffer (Thermo Fisher ...
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bioRxiv - Evolutionary Biology 2022Quote: ... pseudonana expressing VHAB-eGFP were incubated with the acidotropic pH stain 2-(4-pyridyl)-5-((4-(2-dimethylaminoethyl-aminocarbamoyl)methoxy)phenyl)oxazole (PDMPO) (LysoSensor YellowBlue DND-160; Life Technologies) at a final concentration of 0.125 μM without washing in F/2 ...
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bioRxiv - Cancer Biology 2023Quote: ... (4) 30 min incubation in ammonium chloride (NH4Cl) and (5) 4 min incubation in Tissue Autofluorescence Quenching Kit (ReadyProbes, ThermoFisher Scientific).Secondary antibodies were used as follows ...
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bioRxiv - Immunology 2021Quote: ... mixed (1:1 in volume) with Fluo 4 dye (Fluo 4 Direct Calcium Assay Kit, ThermoFisher Scientific) and incubated at 37°C for 30 minutes ...
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bioRxiv - Biophysics 2022Quote: The GyrA14-E487C and the CcdA50-72 peptide (Table S1) were labeled with Fluorescein-5-Maleimide and 5-TAMRA (Tetramethylrhodamine-5-Maleimide) from ThermoFisher Scientific as per the manufacturer’s protocol as described previously (Aghera et al. ...
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bioRxiv - Immunology 2021Quote: ... or control PBS added onto a slant of 2 mL of TSA within a 5 mL round bottom polypropylene tube (Thermo Fisher Scientific Inc., Wilmington, DE, USA) (Supplemental Figure S1) ...
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bioRxiv - Microbiology 2020Quote: ... was used at 1:4000 in 5% milk and exposed with SuperSignal West Dura substrate (Thermo Fisher).
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bioRxiv - Cell Biology 2020Quote: ... cells were fixed for 5 min at room temperature with 1 % methanol-free formaldehyde (Thermo Scientific, #28906). Cells were lysed using ice-cold Farnham lab buffer supplemented with complete protease inhibitor cocktail (#4693159001 ...
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bioRxiv - Cell Biology 2020Quote: ... for 30 minutes at 37°C and 5% CO2 and counterstained with 1:10,000 Hoechst (Thermo Fisher). Parameters were set as follows ...
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bioRxiv - Microbiology 2020Quote: ... 200μl of sample was extracted with a 5:1 volume ratio of TRIzol LS (Ambion, Carlsbad, CA), utilizing standard manufacturers protocols and resuspending in 11μl water ...
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bioRxiv - Cell Biology 2019Quote: ... 1 µL of chromatide ChromaTide™ Alexa Fluor™ 488-5-UTP or 546-14-UTP (Thermofisher) were added to transcription reactions to fluorescently trace label mRNAs ...
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bioRxiv - Bioengineering 2021Quote: ... 5×105 THP-1 cells/ml were treated with 25 ng/ml phorbol myristate acetate (PMA, ThermoFisher) in RPMI 1640 with 10% FBS ...
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bioRxiv - Biochemistry 2021Quote: ... PAs were loaded with X-Rhod-1 AM (5 μM, Thermo Fisher Scientific Inc., Waltham, MA, USA) for 30 minutes at 30°C ...
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bioRxiv - Biochemistry 2020Quote: ... Reactions mixes were first incubated on ice for 5 min before adding 1 µl BMOE (Thermo Fisher) to a final concentration of 0.64 mM ...
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bioRxiv - Cell Biology 2021Quote: ... Mouse ENKD1 shRNA (nucleotides targeting 403-423 bp, 5′-CGCTCACCCAAGTATGACAAT-3′) were cloned into pLKO.1 (Invitrogen).
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bioRxiv - Plant Biology 2020Quote: ... 1 mM EDTA and 5 mM DTT) with the addition of 1μl of Protease Inhibitor (Thermo Fisher) and 1 μl of RNAse inhibitor (RiboLock) ...