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Citations for Thermo Fisher :
7901 - 7950 of 10000+ citations for Hemoglobin High Sensitivity Colorimetric Detection Kit 10 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... 1 μg of RNA for GAS5 was used to synthesize cDNA using high Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA) in a T-Professional Basic ...
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bioRxiv - Microbiology 2019Quote: ... A total of 6 μg of total RNA was reverse-transcribed with random primers using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA). qRT-PCR was conducted using the Applied Biosystems One-Step Real-time PCR protocol using SYBR Green fluorescence (Applied Biosystems ...
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bioRxiv - Plant Biology 2021Quote: ... a small fraction of peptides from all samples was pooled and fractionated using a Pierce High pH Reversed-Phase Peptide Fractionation Kit (Pierce, Thermo Fisher Scientific, San Jose). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... one microgram of RNA was used to prepare cDNA using a High-Capacity RNA-to-cDNA™ Kit (Applied Biosystems; Foster City, CA, USA). From this ...
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bioRxiv - Developmental Biology 2019Quote: ... One μg of RNA was used for cDNA synthesis following the instructions of the “High-Capacity cDNA Reverse Transcription Kit” (ThermoFisher Scientific, Cat. No. 4368814). The cDNA was then diluted 20 times and 5 μl of the dilution was mixed with 2×VeriQuest Fast SYBR Green qPCR Master Mix (Affymetrix ...
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bioRxiv - Neuroscience 2019Quote: ... The RNA concentration was determined via Nanodrop and RNA was reverse transcribed using the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific, Cat# 4368813). Real time PCR was performed on a Applied Biosystems StepOnePlus Real-Time PCR instrument using 2x TaqMan Universal Master Mix (Cat # 4440040 ...
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bioRxiv - Cancer Biology 2019Quote: ... Total RNA (1μg) was used to reverse-transcribe into cDNA using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems/ThermoFisher, Grand Island, NY). Target sequence for ITGB4 shRNA ...
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bioRxiv - Plant Biology 2019Quote: ... 500 ng of total RNA was converted into cDNA in a 20 μl reaction using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Life technologies, Thermo Fisher Scientific) according to manufacturer's instructions and subsequently 1/5 diluted in sterile RNAse and DNAse free water ...
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bioRxiv - Genomics 2021Quote: ... The Fluidigm Access Array system (San Francisco, CA, USA) and SuperScript™ III One-Step RT-PCR kit with Platinum™ Taq High Fidelity polymerase (Thermo Fisher Scientific) were used for full genome amplification ...
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bioRxiv - Cell Biology 2021Quote: ... was generated from 100 ng of total RNA per sample using random hexamers (Applied Biosystems™ High Capacity cDNA Reverse Transcription Kit, catalog number 4374966, Thermo Fisher Scientific) and subsequent quantitative PCR (qPCR ...
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bioRxiv - Genomics 2021Quote: ... High molecular weight DNA was extracted from muscle and fin clip using MagMAX CORE nucleic acid purification kit (Thermo Fisher Scientific, Carlsbad, CA, USA) to produce PacBio continuous longs reads (CLR ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA was first reverse-transcribed using random hexamers and the High Capacity cDNA Reverse Transcription Kit (4368814, Applied Biosystems, Foster City, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... A total of 1 µg of RNA were used for cDNA synthesis with the High Capacity cDNA Reverse Transcription kit (Applied Biosystems, Foster City, USA) in a thermal cycler (Veriti® Thermal Cycler ...
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bioRxiv - Cancer Biology 2021Quote: ... Five hundred ng of DNASE treated RNA was used for the first-strand synthesis using an ABI high-capacity cDNA reverse transcription kit (Applied Biosystems; Foster City, CA). qRT-PCR was carried out using Fast Sybr green (ABI) ...
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bioRxiv - Immunology 2020Quote: ... and total RNA was DNase I treated and reverse-transcribed using the High-capacity cDNA reverse transcription kit (4368813, Applied Biosystems, Life Technologies, UK). Fifty ng of cDNA was used for qRT-PCR ...
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bioRxiv - Immunology 2021Quote: ... A total of 6 μg of total RNA was reverse-transcribed with random primers using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA).
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bioRxiv - Immunology 2021Quote: ... Isolated RNA (300-400 ng depending on experiment) was reverse-transcribed to cDNA using a High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific, Waltham, MA). Gene expression was analyzed using commercial pre-validated gene expression assays and Taqman reagents (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... a total of 200 ng of total RNA was reverse-transcribed into cDNA using High Capacity cDNA Archive Kit (Invitrogen Life Technologies; Cat-No. 4374966). Quantitative PCR (qPCR ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA was converted to cDNA using High-Capacity cDNA Reverse Transcription Kit according to the manufacturer’s instructions (4368814, Applied Biosystems, Thermo Scientific, Waltham, MA). TaqMan™ Array Human Extracellular Matrix & Adhesion Molecules (4414133 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Single-stranded cDNA was synthesized from equal quantities of DNase-treated RNA (75 ng) using a High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher, Waltham, MA, USA). We designed qRT-PCR primers for all three target genes using Primer3Plus (Untergasser et al. ...
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bioRxiv - Neuroscience 2022Quote: ... mRNA was diluted such that 200ng was used to synthesize cDNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Cat. No. 4368814). The reverse-transcription recycling parameters were set as ...
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bioRxiv - Pathology 2022Quote: ... Total RNA (1 μg) was extracted using Trizol and proceed to reverse transcription reactions using the High Capacity cDNA Reverse Transcription kit (Applied Biosystems, Foster City, CA). qRT-PCR was performed on a 7900HT Sequence Detection system (Applied Biosystems ...
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bioRxiv - Microbiology 2022Quote: ... The purified RNA was reverse transcribed into single-stranded complementary DNA (cDNA) using the Applied Biosystems® High-Capacity cDNA Reverse Transcription Kit (Life Technologies, Burlington, Canada) as recommended by the manufacturer ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA synthesis was performed with 200 ng RNA immediately after RNA isolation using a High-Capacity Reverse-Transcription Kit (Applied Biosystems, Foster City, CA) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... First-strand cDNAs were synthesized from 500 ng of total RNA using the High Capacity Reverse Transcription kit (Applied Biosystems, Foster City, CA, USA). The qRT-PCR experiments were performed using 2X USB VeriQuest Fast SYBR Green qPCR Master Mix (Affymetrix ...
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bioRxiv - Plant Biology 2024Quote: ... One μg of RNA was used for a 20-µL first-strand cDNA synthesis reaction using High Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific, Waltham, MA) following the manufacturer’s guidelines ...
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bioRxiv - Pathology 2023Quote: ... 1 μg of total RNA was reverse transcribed in a 20 μl reaction mix using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The concentration of isolated RNA was quantified using a NanoDrop® spectrophotometer and 1-2 µg was used for cDNA synthesis with a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems™, 4368814). qPCR reactions were conducted using a 7500 Real-Time PCR System (Applied Biosystems™ ...
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bioRxiv - Immunology 2024Quote: ... A total of 6 μg of total RNA was reverse-transcribed with random primers using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA).
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bioRxiv - Immunology 2024Quote: ... First-strand cDNA synthesis of Isolated RNA was carried out using random primers and high capacity cDNA reverse transcription kit (Thermo Fisher Scientific, IL, USA). The forward and reverse primers used for SARS-CoV-2 nucleocapsid were 5’-GTAACACAAGCTTTCGGCAG-3’ and 5’-GTGTGACTTCCATGCCAATG-3’ respectively ...
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bioRxiv - Genetics 2024Quote: ... according to the manufacturer’s instructions.1 μg of total RNA was reverse transcribed using the High-Capacity cDNA Archive Kit (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions in 20 μl final volume at 37 °C for 2 hours following a pre-incubation at room temperature for 10 min ...
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bioRxiv - Immunology 2024Quote: ... and 40-100 ng of RNA was used to perform reverse transcription using ABI High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA). The cDNA was used as template for the qPCR reactions using Bio-Rad’s SYBR® Green supermix using QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Immunology 2024Quote: ... Isolated RNA was converted to cDNA in preparation for qPCR using a High-Capacity cDNA Reverse Transcription Kit with RNase Inhibitor (Applied Biosystems, Cat. No. 4374966) following manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: ... Five hundred nanograms of total RNA were used to synthesize cDNA using a High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher, Cat. No. 4368814). Reverse transcription was performed in a Chromo4TM Real-Time PCR Thermocycler (BioRad ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was first reverse-transcribed using random hexamers and the High-Capacity cDNA Reverse Transcription Kit (4368814, Applied Biosystems, Foster City, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... RNA concentrations were determined using ThermoScientific NanoDrop One and RNA was converted to cDNA using a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Catalog number: 4368814). The qPCR reactions were performed using TaqMan Gene Expression Master Mix (Applied Biosystems ...
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bioRxiv - Physiology 2023Quote: ... The amount of 1 μg of total RNA was used to synthesize cDNA by using High-Capacity cDNA Reverse Transcription kit (#4368814, Applied Biosystems, Thermo Fisher Scientific). Quantitative real-time PCR was performed using TaqMan Gene Expression Master Mix (#4369016 ...
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bioRxiv - Physiology 2023Quote: ... A total of 6 μg of total RNA was reverse-transcribed with random primers using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA).
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bioRxiv - Cell Biology 2023Quote: ... each peptide sample (approximately 2 mg) was tapplied to a Pierce High-Select™ TiO2 Phosphopeptide Enrichment Kit (Thermo Fisher Scientific, Cat No: A32993). After preparing spin tips as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... peptides were reconstituted in 0.1% trifluoroacetic acid and fractionated on Sep-Pak® Vac cartridges using methodology and reagents from Pierce™ High pH reversed-phase peptide fractionation kit (8 fractions for global proteomics and 4 for phosphoproteomics skipping every other; Thermo Fisher Cat No: 84868). Samples were run (1/8th of each global and 1/5th of each phosphopeptide fraction ...
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bioRxiv - Microbiology 2023Quote: ... the extracted RNA was subjected to reverse transcription to complementary DNA (cDNA) using a high-capacity RNA-to-cDNA kit (4387406, Thermo Fisher Scientific Baltics UAB) and a SimpliAmp Thermal Cycler (A24811 ...
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bioRxiv - Neuroscience 2023Quote: ... 2 μg of total RNA was reverse transcribed into cDNA using a High-Capacity RNA-to-cDNA kit (#4387406, Applied Biosystems, Waltham, MA, USA) and sequences were amplified using Taq DNA polymerase that had been supplied with a PCR master mix (#4324018 ...
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bioRxiv - Microbiology 2023Quote: ... A total of 1 μg of RNA was incubated with DNase I for 15min and reverse transcribed with the High Capacity cDNA Reverse Transcription Kit (Thermo Fisher, Waltham, MA USA). The resulting cDNA was used for qPCR ...
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bioRxiv - Developmental Biology 2023Quote: ... Approximately 250ng of total RNA was converted to cDNA in a 20 μL reverse transcription reaction using the Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit (ThermoFisher Scientific, Waltham, MA). The reaction involved ...
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bioRxiv - Biochemistry 2023Quote: ... Reverse transcription was performed using a High-Capacity cDNA Reverse Transcription kit and 200 ng of total RNA (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was reverse-transcribed from 500 ng of RNA using the high capacity RNA to cDNA kit (Cat # 4387406) following the manufacturer’s protocols (Applied Biosystems, Foster City, CA, USA) and amplified by quantitative real time PCR using forward and reverse primers (Integrated DNA Technologies ...
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bioRxiv - Immunology 2023Quote: ... total RNA was quantified using a Nanodrop and 1 μg of RNA was used to perform the reverse transcription using a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Waltham, MA, US). 50 ng of the resulting cDNA was amplified in a 7500 Real-Time PCR system (Applied Biosystems ...
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bioRxiv - Biochemistry 2023Quote: ... Pre-incubated mixture of scFv and respective whole venom was mixed with high-performance His-tag binding cobalt resin beads provided with Pierce™ His Protein Interaction Pull-Down Kit (Thermo Fisher Scientific, USA) and incubated at 20°C for 12 hours to allow attachment of the complex on resin via His-tag ...
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bioRxiv - Developmental Biology 2023Quote: ... A total of 1 μg of RNA per reaction was reverse-transcribed using a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Thermo Fisher Scientific, 4368814). The following primers were used:
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bioRxiv - Neuroscience 2023Quote: Reverse transcription for individual qPCRs was carried out using 500 ng (or maximum volume) of RNA and the High Capacity RNA-to-cDNA Kit (Applied Biosystems, Foster City, CA) using specific primers for miR-324-5p ...