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Citations for Thermo Fisher :
7701 - 7750 of 10000+ citations for TRAP 5 amide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... 5’-\56-FAM\ CCT ACC TTA ACC TCC C-3’ with Minor Groove Binder (MGB; ThermoFisher Scientific). PCR was performed using 10X Master Mix to yield a final volume of 25 µl and final working concentrations of 16.6mM (NH4)2SO4 ...
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bioRxiv - Cancer Biology 2021Quote: ... Biological duplicates (5 x 106 cells per replicate) were homogenized in 1 ml of TRIzol Reagent (Invitrogen) and purified using the mirVANA miRNA isolation kit (Ambion ...
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bioRxiv - Biochemistry 2020Quote: ... The ThT fluorescence signal was measured every 5 min using the Varioskan plate reader (Thermo Scientific, Inc.) or FLUOstar Omega plate reader (BMG Labtech ...
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bioRxiv - Cancer Biology 2020Quote: ... ANA-1 cells were incubated in serum-free media with CellTracker Red CMTPX Dye (5 μM, ThermoFisher) for at 37 °C for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were maintained at 37°C with 5% CO2 and culture in F-12K media (Gibco, #21127), with 10% horse serum and 5% fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2021Quote: gDNA was harvested from 5 million cells for WT and Cdc42-KOclones (Invitrogen Purelink Genomic DNA Kit). The purified gDNA was then used as a PCR template for primers that flank the CRISPR cut site ...
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bioRxiv - Biophysics 2020Quote: ... 5% CO2 atmosphere in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% Penicillin-Streptomycin (Pen-Strep ...
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bioRxiv - Cell Biology 2021Quote: ... 5% CO2 and regularly passaged in DMEM media supplemented with 10% (vol/vol) Fetal Bovine Serum (Gibco) and 1% (vol/vol ...
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bioRxiv - Cell Biology 2020Quote: ... MCF10A and its derivatives were cultured in DMEM/F12 supplemented with 5% horse serum (GIBCO, #16050-122), 20 ng/ml EGF (Peprotech ...
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bioRxiv - Cell Biology 2021Quote: ... The sample was loaded onto the trapping column (Thermo Scientific, PepMap100, C18, 300 μm x 5 mm), for 7 minutes at a flow rate of 9 μL/min with 0.1% TFA and 2% acetonitrile ...
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bioRxiv - Cell Biology 2020Quote: ... Sections were thawed at room temperature and washed 3 times 5 minutes in DPBS (Gibco, 14190-094). Sections were then counterstained for 5 minutes with Hoechst 33342 (1μg/mL ...
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bioRxiv - Cell Biology 2021Quote: ... Per3 (QT00133455) or Wee1 (QT00157696) using a QuantStudio 5 Real time PCR system (Thermo Fisher Scientific, A28140) in a 384 well plate setting (final reaction volume 10μl per well) ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated for 30 min in blocking buffer (3.75% BSA and 5% goat serum, Thermo Fisher Scientific). Samples were then immunolabeled for Alpha-2C adrenergic receptor ...
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bioRxiv - Cell Biology 2021Quote: ... a separate sample of cells was stained with 5 μM Vybrant® DyeCycleTM Violet Stain (V35003, Invitrogen) in full medium for 30 minutes and kept in vibrant containing medium ...
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bioRxiv - Cell Biology 2021Quote: ... and either maintained at 37°C with 5% CO2 or incubated in CO2-independent medium (Life Technologies). Z-stacks of images separated by 500 nm were collected at a minimum of 3-min intervals using a 1.35 na 100X objective ...
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bioRxiv - Cancer Biology 2022Quote: ... Met-5A monolayer were labelled with 5 µM of CellTracker™ Orange CMRA Dye (ThermoFisher Scientific #C34551) and cancer cell spheroids were labelled with 10 µM of CellTracker™ Green CMFDA Dye (ThermoFisher Scientific #C7025 ...
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bioRxiv - Cell Biology 2022Quote: C2C12 cells were incubated with CM containing 5 μM BODIPY 558/568 C12 (Red C12, Life Technologies) for 16 hr ...
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bioRxiv - Cell Biology 2022Quote: ... The labeled UTPs used were ChromaTide Alexa Fluor 488-5-UTP (Thermo Fisher Scientific, product number C11403), Cy3-UTP (Cytiva ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were maintained at 37°C 5% CO2 in Dulbecco’s MEM (Life Technologies, ThermoFisher Scientific, Waltham MA) with 10% FBS ...
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bioRxiv - Microbiology 2022Quote: ... Protein lysates were boiled at 95 °C for 5 mins in 6x SDS sample buffer (Thermo Scientific). Proteins were separated on 4-12% Bis-Tris SDS-PAGE gels (Thermo Fisher ...
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bioRxiv - Neuroscience 2022Quote: ... 5′-CCCAAGACTTCCACCTACATTC -3′ (Tm = 62°C) and subsequently subcloned into PCR II-TOPO-TA cloning vector (Invitrogen). Riboprobes were generated with a BIOT-NTP labeling kit (Roche) ...
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bioRxiv - Molecular Biology 2022Quote: ... single islets were mounted directly onto a slide with 5 µL of ProLong (Molecular Probes, Eugene, OR) and a coverslip ...
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bioRxiv - Cell Biology 2022Quote: ... MRC-5 cells (ATCC) were cultured in EMEM supplemented with 10% FBS and 1% Penicillin/Streptomycin (Gibco).
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bioRxiv - Neuroscience 2022Quote: ... in PBS for 10min and then blocked for 30 minutes in 5% goat serum 2.5% BSA (Gibco) 0.1% Triton X in PBS ...
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bioRxiv - Microbiology 2022Quote: ... Primers used to amplify the 5’/3’ junction with Phusion high-fidelity DNA polymerase (Thermo Fisher Scientific) are listed in Table S3 ...
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bioRxiv - Microbiology 2022Quote: ... Data analysis was performed with the Quantstudio 3&5 Design and analysis software (version v1.5.1, Applied Biosystems). Cq values were obtained via automated threshold determination ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were resuspended to a concentration of 5 × 106 cells/mL and DAPI (Invitrogen Cat. No. D1306) was added to the cells at a concentration of 10 μg/mL ...
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bioRxiv - Immunology 2022Quote: ... resuspended in pre-sort buffer containing 3-5 nM SYTOX Green Nucleic Acid Stain (Thermo Fisher Scientific), and incubated for 20 min at 4°C ...
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bioRxiv - Bioengineering 2022Quote: ... and blocked for 1 hr with a solution containing 0.5% TritonX-100 and 5% donkey serum (ThermoFisher). Primary antibodies used were rat CD68 (FA-11 ...
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bioRxiv - Developmental Biology 2022Quote: ... washed five times for 30 minutes each with PBS and incubated with 5% normal goat serum (ThermoFisher) and 0.5% TritonX (Sigma Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... For phosphorylation of the 5′ hydroxyl terminus of PCR amplicons and oligonucleotides T4 Polynucleotide Kinase (Thermo Scientific) was applied ...
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bioRxiv - Developmental Biology 2022Quote: PCR products were used as templates for T7 transcription reactions with the 5× MEGAscript T7 kit (Ambion). dsRNA was injected dorsally in 0-1 hour old embryos from the stocks w ...
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bioRxiv - Biochemistry 2022Quote: ... and both were cultured at 37°C under 5% CO2 in Dulbecco’s modified Eagles medium (DMEM, Gibco) supplemented with 10% (vol/vol ...
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bioRxiv - Immunology 2022Quote: ... 5 mL of MEM medium with 10% FBS and 1X Antibiotic-Antimycotic (Thermo Fisher Scientific, cat: 15240062) was added directly ...
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bioRxiv - Biochemistry 2022Quote: ... HEK293-PSMB2-HB cells were transfected with 5 μg of the cloned plasmid using Lipofectamine 3000 (Invitrogen). After 48 h incubation ...
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bioRxiv - Cancer Biology 2022Quote: ... The 5-µm FFPE tissue section was mounted on positively charged Superfrost glass slide (Thermo Fisher Scientific) and baked for 30 mins at 60 °C ...
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bioRxiv - Genomics 2022Quote: ... These cells were cultured at 37°C and 5% CO2 in reprogramming medium containing KnockOut DMEM (GIBCO), 10% knockout serum replacement (KSR ...
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bioRxiv - Developmental Biology 2022Quote: ... single cell suspensions were centrifuged and resuspended in 5 mL of red blood cell lysis buffer (Invitrogen) for 5 min ...
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bioRxiv - Cell Biology 2022Quote: ... Antibodies for immunofluorescent staining were as follows: vinculin (clone VIN-11-5, Sigma or clone 42H89L44, Invitrogen); phosphotyrosine (clone 4G10 ...
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bioRxiv - Developmental Biology 2022Quote: ... at 95°C for 5 min and separated using NuPAGE 4%–12% Bis-Tris Protein Gel (Invitrogen) in NuPAGE MES SDS Running Buffer (Invitrogen) ...
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bioRxiv - Cancer Biology 2022Quote: ... real-time quantitative PCR was done using the ABI Quant Studio 5 and 6 (Life technologies, USA). The cDNA was used as template and DyNAmo Flash SYBR Green qPCR kit (#F-416L ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were incubated at 37°C in humidified 5% CO2 incubators (Eppendorf or Thermo Fisher Scientific, USA).
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bioRxiv - Cancer Biology 2022Quote: ... Cells were maintained at 37°C 5% CO2 in Dulbecco’s MEM (Life Technologies, ThermoFisher Scientific, Waltham MA) with 10% FBS ...
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bioRxiv - Cell Biology 2022Quote: ... about ∼5 μg of bacmid were transfected using 6 μl of Cellfectin II reagent (Thermo Fisher Scientific). 5 days after initial transfection ...
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bioRxiv - Cancer Biology 2022Quote: ... MS-5 cells were seeded into 0.2% gelatin coated 96 well-flat bottom plates (Thermo Fisher, 167008) at a density of 4-5×105 cells per well in Myelocult medium (H5100 ...
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bioRxiv - Neuroscience 2021Quote: ... The template cDNA was reverse transcribed with 5’-RACE RT primer and SuperScript III reverse transcriptase (Invitrogen), followed by poly(A ...
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bioRxiv - Developmental Biology 2020Quote: ... Tissue was dissociated for 5 minutes at 37°C in TrypLE Express Enzyme reagent (Thermo Fisher 12604013), with shaking ...
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bioRxiv - Microbiology 2020Quote: 5×105 293T cells were seeded and the next day transfected using Lipofectamine 3000 (Thermo Fisher Scientific) with 3 μg of E.V. ...
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bioRxiv - Immunology 2020Quote: ... 0.1 μl of External RNA Controls Consortium (ERCC) spike-in mix (10-5 diluted; Life Technologies, 4456740) was added with 0.1 μl of 100 μM oligo-dT primer and 1 μl of dNTP mix and 0.8 μl of H2O ...
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bioRxiv - Biophysics 2020Quote: ... A 5% solution of 0.2 μm diameter red fluorescent carboxylate-modified microspheres (FluoSpheres, Invitrogen, Carlsbad, CA, USA) in PBS was vortexed for 10 seconds ...