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Citations for Thermo Fisher :
7601 - 7650 of 10000+ citations for hsa mir 23b Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... The gag-protease region was amplified by reverse transcription (RT)-PCR from plasma HIV-1 RNA using Superscript III One-Step Reverse Transcriptase kit (Invitrogen) and the following gag-protease -specific primers ...
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bioRxiv - Pathology 2020Quote: Real-time RT-PCR studies were performed on cDNA samples using the Platinum qPCR SuperMix-UDG kit (Catalog No.11730-017, Invitrogen). The HIV-envelope assay was designed to amplify a 95-bp amplicon from HIV-1 NL4-3 (GenBank accession no ...
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bioRxiv - Immunology 2020Quote: ... The detection and measurement of SARS-CoV-2 viral load were performed using COVID-19 RT-PCR diagnostic kit with specific Taqman probes (ThermoFisher) of SARS-CoV-2-specific genes encoded by the RNA-dependent RNA polymerase (RdRp/ORF1ab) ...
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bioRxiv - Microbiology 2021Quote: ... assay using the OPTI Medical SARS-CoV-2 RT-PCR kit following the manufacturer’s instructions on an ABI 7500 Fast instrument (ThermoFisher Scientific). The OPTI Medical SARS-CoV-2 RT-PCR assay detects two different targets in the gene encoding viral nucleocapsid (N ...
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bioRxiv - Microbiology 2021Quote: ... was synthesized from isolated RNA (1 µg) using the SuperScript III First-Strand Synthesis System for RT-PCR kit (Invitrogen), following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 2.5 uL of extracted RNA were used as a template in a 25 uL MS-RTPCR reaction (Superscript III high-fidelity RT-PCR kit, ThermoFisher); using Opti1-F1 (0.06 uM) ...
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bioRxiv - Genomics 2022Quote: ... The RNA extracts were used as templates with the Superscript III One-Step RT-PCR System with Platinum Taq DNA polymerase kit (Invitrogen) following the manufacturer’s instructions and primers designed to detect the jingmenvirus segments ...
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bioRxiv - Molecular Biology 2022Quote: ... The unspiked and spiked samples were then subjected to amplification using the Verso 1-step RT-PCR kit (Thermo Scientific); the reaction was set up in 10 μl final volumes with 600 nM of labelled triplex primer cocktail and 1-3 μl of RNA template ...
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bioRxiv - Physiology 2023Quote: ... Tissue was lysed in 500 µl lysis buffer from the RNAqueous-4PCR DNA-free RNA isolation for RT-PCR kit (Ambion). RNA was extracted from all samples following manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... tuberculosis cultures grown under specific conditions were used for cDNA synthesis and PCR reactions using Superscript III platinum-SYBR green one-step RT-qPCR kit (Invitrogen). Details of PCR conditions are described previously (Goar et al. ...
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bioRxiv - Cell Biology 2023Quote: ... Coding sequences were amplified using total RNA from 7 days post-fertilization (dpf) zebrafish using the SuperScript IV One-step RT-PCR kit (ThermoFisher) using the primers listed in Table S1 ...
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bioRxiv - Plant Biology 2024Quote: ... cDNA was synthesized using the SuperScript III First-Strand Synthesis System for the RT-PCR kit (Invitrogen, San Diego, CA), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... RT-Q-PCR was performed with the Power SYBR™ Green RNA-to-CT™ 1-Step Kit (Thermofisher Scientific). Primers were designed to target different genes of interests (e.g ...
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bioRxiv - Microbiology 2024Quote: ... with the MBTuni-12 [5’-ACGCGTGATCAGCAAAAGCAGG] and MBTuni-13 [5’-ACGCGTGATCAGTAGAAACAAGG] primer sets for Influenza A (59, 60) with the SuperScript III One-Step RT-PCR kit as per manufacturers guidelines (Invitrogen).
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bioRxiv - Immunology 2023Quote: ... The HSA-IgG complexes were immobilized on the albumin depletion column using the Pierce Albumin Serum Depletion Kit (Thermo Fisher Scientific) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... in the 7500 Fast Real Timer PCR system (Applied Biosystems®). SNORD47 and SNORD48 or HSP90 and RPS13 were used as reference endogenous controls to assess the expression of miRs and mRNAs ...
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bioRxiv - Cell Biology 2021Quote: ... and miR-34a-5p (ThermoFisher, Assay ID 000426). 1ul of cDNA was used for real-time PCR using TaqMan™ Universal PCR Master Mix (Applied Biosystems ...
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bioRxiv - Developmental Biology 2022Quote: ... for mature miR-9 or SuperScript III (Invitrogen) with random hexamers for pri-miRNAs ...
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bioRxiv - Molecular Biology 2019Quote: ... Anti-mir-125b was obtained from Applied Biosystems. mPAPD2 was PCR amplified from PASMC cDNA using the following primers ...
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bioRxiv - Neuroscience 2021Quote: ... the pcDNA6.2-GW/EmGFP-miR-neg plasmid (Invitrogen), containing a sequence not targeting any known vertebrate gene ...
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bioRxiv - Cell Biology 2020Quote: ... mirVana miR mimic negative control (Cat: 4464061, Ambion), hsa-miR-494-3p mimic (MIMAT002816 ...
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bioRxiv - Cell Biology 2022Quote: ... using the Human pre-miR miRNA library (Ambion), consisting of 837 miRNAs ...
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bioRxiv - Cell Biology 2023Quote: ... and Anti-miR™ miRNA Inhibitor (AM17000, ThermoFisher) were co-transfected by Lipofectamine RNAiMAX Transfection Reagent (13778100 ...
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bioRxiv - Cancer Biology 2024Quote: ... mmu-miR-183 (Thermo Fisher, assay id 002269), and U6 snRNA (Thermo Fisher ...
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bioRxiv - Cancer Biology 2024Quote: ... mir-96 mimic and antagomir (Ambion, nocodazole (CST). For Western immunoblots the following antibodies were used ...
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bioRxiv - Microbiology 2022Quote: ... Amplification and detection of PCR products was performed with a StepOne Plus qRT-PCR system (Applied Biosystems) using the following procedure ...
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bioRxiv - Cell Biology 2019Quote: ... which was then used to perform quantitative RT-PCR (qRT-PCR) using SYBR Green (Applied Biosystems, 4385617) for the specified target genes (Table S3).
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bioRxiv - Neuroscience 2020Quote: ... Semi-quantitative RT-PCR reactions were performed using DreamTaq Green PCR Master Mix (Thermo Fisher Scientific-K1081) on an Eppendorf thermocycler ...
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bioRxiv - Microbiology 2021Quote: ... Quantitative RT-PCR assays were setup using the Power SYBR Green PCR Master Mix (Applied Biosystems, UK) using 250 nM of primer pairs with cycling conditions ...
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bioRxiv - Plant Biology 2021Quote: ... a standard PCR (RT-PCR) was then performed using DreamTaq DNA polymerase (5 U/μL) (Thermo Scientific). VIGS-CHUP1 silencing was confirmed by separately amplifying CHUP1a and CHUP1b from cDNA with the following primer pairs:
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bioRxiv - Plant Biology 2021Quote: ... a standard PCR (RT-PCR) was then performed using DreamTaq DNA polymerase (5 U/μL) (Thermo Scientific). VIGS BAK1 silencing was confirmed as previously described (Chaparro-Garcia et al. ...
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bioRxiv - Immunology 2019Quote: ... and amplified with the Superscript III platinum one-step quantitative reverse-transcription-PCR (RT-PCR) system (Invitrogen). The detection limit of the assay was 100 vRNA copy equivalents per mL of plasma (copies/mL) ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative RT-PCR was performed using the Taqman Fast Universal PCR Master Mix (Applied Biosystems, Cat# 4352042) or Sybr Green PCR Master kit (Applied Biosystems ...
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bioRxiv - Cell Biology 2024Quote: ... RT-PCR for gel electrophoresis was performed using Platinum II Hot-Start Green PCR Master Mix (ThermoFisher), and products separated on 2% EX e-gels containing SYBR Gold II (Invitrogen) ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative PCR of RT products (qRT-PCR) was conducted using VeriQuest SYBR Green qPCR Master Mix (Affymetrix). Peptidylprolyl isomerase B (Ppib ...
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bioRxiv - Cell Biology 2024Quote: ... Quantitative RT-PCR was performed with TaqMan fast universal PCR master mix (Applied Biosystems; Foster City, CA). RT reaction conditions ...
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bioRxiv - Immunology 2024Quote: ... Quantitative RT-PCR (qRT-PCR) analyses were conducted on a StepOnePlus system (Applied Biosystems, Foster City, CA) employing SYBR Premix Ex Taq (Takara Bio) ...
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bioRxiv - Cell Biology 2023Quote: ... RT-PCR and quantitative PCR (qPCR) were performed using an ABI7500 qPCR instrument (Life Technologies, Carlsbad, CA) and a PrimeScript RTreagent kit with gDNA Eraser and SYBR Premix Ex Taq II (TliRNase H Plus ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative RT-PCR was performed using the Taqman Fast Universal PCR Master Mix (Cat# 4352042, Applied Biosystems) and a StepOnePlus real-time PCR system (Applied Biosystems) ...
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bioRxiv - Developmental Biology 2024Quote: ... Quantitative RT-PCR analysis was performed using Power SYBR Green PCR Master Mix (Applied Biosystems, Life Technologies) and an Applied Biosystems ABI 7500 Real-Time PCR system using the primers listed in the supplementary material ...
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bioRxiv - Developmental Biology 2024Quote: ... Quantitative RT-PCR analysis was performed using Power SYBR Green PCR Master Mix (Applied Biosystems, Life Technologies) and an Applied Biosystems ABI 7500 Real-Time PCR system using the primers listed in the supplementary material ...
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bioRxiv - Neuroscience 2020Quote: ... Transcripts abundance was determined by reverse transcription quantitative PCR (qRT-PCR) using the SYBR® Green RT-PCR assay (Applied Biosystems). Amplification ...
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bioRxiv - Systems Biology 2021Quote: EdU detection was performed using the Click-iT 647 EdU detection kit (Thermo Fisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2022Quote: ... Detection was performed using the SuperSignal West Femto Maximum Sensitivity Substrate detection kit (ThermoFisher Scientific). Image capture was done using the image analyzer Amersham 600RGB (GE Healthcare) ...
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bioRxiv - Neuroscience 2022Quote: ... High-Capacity cDNA Reverse Transcription Kit and Mir-X™ miRNA First Strand Synthesis Kit were purchased from Applied Biosystems (USA) and Takara (China ...
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bioRxiv - Cell Biology 2021Quote: L1CAM knockdown neurons were prepared by using a Block-iT Pol II miR RNAi expression kit (Invitrogen). The targeting sequence of L1CAM miRNA #1 ...
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bioRxiv - Neuroscience 2022Quote: ... miRNA plasmids were prepared using the BLOCK-iT Pol II miR RNAi Expression Vector Kit (ThermoFisher Scientific) with top and bottom sequences targeting mouse Rxra cDNA (Top ...
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bioRxiv - Evolutionary Biology 2019Quote: Total RNA was extracted with a mir™Vana Kit (#AM1560; Thermo Fisher scientific, Waltham, MA, USA) from frozen C ...
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bioRxiv - Physiology 2022Quote: ... and reverse transcribed using the High Capacity cDNA RT Kit minus RT inhibitor (Applied Biosystems) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Further RT-qPCR was performed using a Verso 1-step RT-qPCR kit (Invitrogen, USA) as previously described [5] ...