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Citations for Thermo Fisher :
7601 - 7650 of 10000+ citations for C Reactive Protein Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Cells were then washed three times with PBS and incubated for 1 h at room temperature with secondary antibodies diluted in blocking buffer (Alexa fluor (AF) 488 donkey anti-rabbit (1:500; ThermoFisher Scientific), AF568 donkey-anti-rat (1:500 ...
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bioRxiv - Cancer Biology 2023Quote: ... TRAIL blocking ab was purchased from Santa Cruz TRAIL Antibody (RIK-2): sc-56246, and IFN gamma blocking Monoclonal Antibody (NIB42, #6-7318-81) was purchased from Thermo Fisher. The blocking mAb was added when NK cells were combined with tumor cells.
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bioRxiv - Cell Biology 2024Quote: ... were added at a dilution of 1:500 in blocking solution (Aligent Antibody Diluent #S202230-2) containing 1:100 Phalloidin 594 (ThermoFisher #A12381) to cells and incubated at 4 °C overnight ...
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bioRxiv - Biophysics 2024Quote: EDTA-containing D-PBS (blocking buffer)) and a goat anti-mouse IgG secondary antibody conjugated with Alexa Fluor 488 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... incubated in blocking buffer (3X SSC supplemented with 2% BSA, 0.1% Triton X100 and 2U/ul RNAse inhibitor (RiboLock, Thermofisher, Cat# EO0384)) ...
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bioRxiv - Cell Biology 2024Quote: ... and then incubated with one or more of the following secondary antibodies in blocking solution for 30 minutes (Donkey anti-rabbit Alexa 488 [A21206; Fisher Scientific] ...
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bioRxiv - Bioengineering 2024Quote: ... and then incubated with the secondary antibodies donkey anti-goat IgG Alexa Fluor™ 647 (1:500 in PBS blocking solution, A-21447, Invitrogen) and donkey anti-rabbit IgG Alexa Fluor™ 488 (1:400 in PBS with 0.05% tween ...
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bioRxiv - Immunology 2024Quote: ... slides were washed with 1:3 diluted blocking buffer (3-times, 1 minute) and incubated with anti-mouse Alexa fluor-594 antibody (Invitrogen, A21203), anti-rabbit Alexa fluor-750 (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then blocked for one hour at room temperature with a blocking reagent 2% bovine serum albumin (BSA; Fisher Scientific) in PBS followed by staining with primary antibodies ...
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bioRxiv - Microbiology 2024Quote: ... Cells were washed 4 times with PBS and then incubated with secondary antibody (goat α-mouse AlexaFluor 488 diluted 1:100 in blocking solution, Invitrogen) in the presence of 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Samples were incubated overnight in a blocking buffer and primary antibody solutions containing the following: mouse anti-IBA1 (Invitrogen, 1:250), rabbit anti-IBA1 (Abcam ...
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bioRxiv - Developmental Biology 2024Quote: ... We first incubated whole pre-fixed tissue in 1ml blocking solution (ice-cold PBS solution with 10% (v/v) Heat Inactivated Sheep Serum (HISS) (ThermoFisher, 16070096) and 0.5% (v/v ...
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bioRxiv - Neuroscience 2021Quote: ... the membranes were probed with the following primary antibodies at 4°C overnight: mouse anti-EphA4 C-terminus (Invitrogen, USA; 1:1000), goat anti-EphA4 N-terminus (R&D Systems ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Samples are frozen for at least 10 min at −80°C and then immediately centrifuged for 90 min at 4°C and 13000 rpm (Heraeus™ Biofuge fresco, Thermo Scientific). After centrifugation three different stable phases are obtained ...
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bioRxiv - Cell Biology 2021Quote: ... with or without inhibitors at 4°C for 5 min and 37°C for 30 min in the presence of FM4-64FX (Thermo Fisher Scientific) to stain wounded cells ...
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bioRxiv - Biochemistry 2021Quote: ... followed by 10 cycles of 5’ 60 °C -> 5’ 24 °C (HDV cleavage of construct to release pure R3C) and DNAse I treatment (Thermo Scientific, USA). 75-85 nt randomised oligomer was expressed using artificial DNA template (Eurofins Genomics ...
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bioRxiv - Bioengineering 2019Quote: ... followed by 40 cycles at 95° C for 3 s and 60° C for 20 s (7500 Fast Real-Time PCR System; Thermo Fisher Scientific). Taqman probes for Gnat1 (Mm01229120 ...
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bioRxiv - Microbiology 2021Quote: ... followed by 30 minutes with 0.1% Tween/PBS at 37°C and 1 hour at 37°C with secondary antibody Alexa-488 goat anti-mouse (1:500) (Life Technologies #A11029) in 1% BSA/PBS before mounting with Prolong Gold with DAPI (Invitrogen #P36935) ...
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bioRxiv - Microbiology 2020Quote: ... 72°C for 1 min and a final extension at 72°C for 10 min in a thermal cycler (Applied Biosystems Veriti). Deletions in the PCR products were visualized by gel electrophoresis and confirmed by Sanger sequencing ...
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bioRxiv - Microbiology 2019Quote: ... and heated for 2 minutes at 85°C or 95°C before gel electrophoresis on a precast 10-20% Tricine gel (Thermo Fisher Scientific) in Tricine running buffer (100 mM Tris base [pH 8.3] ...
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bioRxiv - Neuroscience 2019Quote: ... Supernatant was kept after spinning at 800 rcf for 2’ at +4°C and were incubated overnight at +4°C with 3µg of dedicated antibodies: Nr2f1 antibody (Thermo Fisher PA5-21611) and GFP antibody (Abcam ab13970 ...
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bioRxiv - Biochemistry 2021Quote: ... RBD-C-tag was purified from 116 h time point of the fermentation by C-tag affinity chromatography with the CaptureSelect C-tagXL resin (Thermo Fisher Scientific) according to the manufacturer’s protocols ...
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bioRxiv - Neuroscience 2022Quote: ... 72°C for 5 minutes and then held at 4°C in a Veriti™ 96-well thermal cycler (Applied Biosystems™). Adaptors from the LSK-109 sequencing kit (Nanopore ...
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bioRxiv - Molecular Biology 2022Quote: ... from intron 37 to intron 41 containing the c.5461-10T>C mutation were digested with BoxI and BsiWI (Thermo Fisher Scientific) and ...
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bioRxiv - Developmental Biology 2021Quote: ... and frozen overnight at -80 °C in a NALGENE Cryo 1 °C freezing container (5100-0001, ThermoFisher Scientific, Hanover Park, IL, USA) filled with isopropanol (423830025 ...
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bioRxiv - Molecular Biology 2022Quote: ... 95°C for 20sec then 40 cycles of 95°C for 3sec and 58°C for 45sec on the QuantStudio 5 and 7 qRT-PCR systems (Applied Biosystems, CA). A known negative sample and non-template control (NTC ...
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bioRxiv - Neuroscience 2022Quote: ... 2 minutes at 80°C and then held at 20°C in a Veriti™ 96-well thermal cycler (Applied Biosystems™) until cleavage.
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bioRxiv - Neuroscience 2022Quote: ... supplemented with 5% Fetal Bovine Serum (FBS), 4°C) and incubated (37°C, 2 minutes) in protease solution (TrypLE express, Thermo Fisher 12604013) supplemented with DNase (100 U/ml ...
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bioRxiv - Microbiology 2022Quote: ... Plates were incubated at 4°C overnight and then blocked for 1 hour at 37°C using SuperBlock (ThermoFisher Scientific, Cat. # 37516), and then washed four times with PBS 0.05% Tween-20 (PBST) ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were subjected to a heating ramp from 25°C to 95°C at a rate of 1°C/s using the ABI 7500 Fast Real-Time PCR System (Applied Biosystems, USA). Data was analyzed by Protein Thermal Shift Software v1.4 to obtain melting temperature (Tm).
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bioRxiv - Cell Biology 2023Quote: ... The lysates were digested overnight at 37°C with 1-2 µL of the Lys-C (125-05061, Wako) and trypsin mixtures (Pierce, Thermo Fisher Scientific) (10 ng/μL each) ...
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bioRxiv - Immunology 2023Quote: ... tumor necrosis factor alpha (TNFα) and C-C motif chemokine ligand 2 (CCL2) concentrations were evaluated using the Ready-SET-Go!™ ELISA kits (ThermoFisher Scientific). CCL24 concentrations were evaluated using the Mouse CCL24/Eotaxin-2/MPIF-2 DuoSet ELISA kit (R&D Systems) ...
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bioRxiv - Bioengineering 2024Quote: ... The frozen organoids were sectioned at 8 µm-thick slices on a Leica CM3050S cryostat (-18°C chamber, - 20°C object, 3.5° blade angle) and mounted to silanated microscope slides (Superfrost Plus, Fisher Scientific, Hampton, NH). The slides with sectioned organoids were dried under laminar flow at room temperature and kept at -80°C until staining.
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bioRxiv - Microbiology 2023Quote: ... and 40 cycles of PCR (95°C for 3 seconds, 60°C for 30 seconds) on a QuantStudio 3 (Applied Biosystems, MA).
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bioRxiv - Immunology 2023Quote: ... which encodes for a C-terminal C-Direct tag containing a free thiol (cysteine) and an EPEA (Glu, Pro, Glu, Ala) purification tag (C-tag, Thermo Fisher Scientific). Vectors were transformed into yeast cells (Saccharomyces cerevisiae strain VWK18 (51)) ...
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bioRxiv - Neuroscience 2023Quote: ... We cooled water to 4 °C (Yeosen, PH-F3) and heated water to ∼55 °C using a hot plate (Fisher Scientific, HP88854200) set to 170 °C ...
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Engineering synthetic CopT/A-based genetic circuits for miRNA imaging and functional gene regulationbioRxiv - Synthetic Biology 2023Quote: ... 40 cycles of 94 °C for 30 s and 60 °C for 30 s with ABI 7700 PCR system (Applied Biosystems, USA). U6 snRNA was used as internal control ...
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bioRxiv - Immunology 2023Quote: ... followed by 40 cycles at 95°C for 15 sec then 60°C for 1 min using a QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific). Extracted MAC DNA was used as the quantification standard.
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bioRxiv - Molecular Biology 2023Quote: Visceral fat was flash frozen in liquid nitrogen and then kept at -80°C until thawed at -20°C in RNAlater (ThermoFisher, catalog # AM7030). The tissue was then homogenized in QIAzol Lysis Reagent (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... RT-qPCR amplification was performed at 95 °C for 2 minutes and subsequently 40 cycles of 95 °C for 2 s and 60 °C for 20 s on a QuantStudio Real-Time PCR System (Thermo Fisher Scientific). The relative expression of different target RNAs in RNA samples isolated with different methods was analyzed using their Ct values in RT-qPCR ...
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bioRxiv - Cancer Biology 2022Quote: ... The oligos were annealed in a thermocycler with gradual T reduction from 95°C to 25°C at a rate of 5°C/min and subsequently diluted 1:20 into Nuclease free water (ThermoFisher Scientific AM9938). The pLentiCRISPRv2 plasmid was digested for 1 hr at 55°C with 1U per μg of DNA BsmBI-v2 (NEB ...
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bioRxiv - Plant Biology 2023Quote: ... Extracted protein samples were centrifuged at 20,000 g for 2 min at 4°C and the supernatant was collected for immunoprecipitation using the Pierce™ Anti-c-Myc Magnetic Beads (88843; Thermo-Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... into the reaction mix and incubating it at 37 °C for 15 min and 98 °C for 5 min in the Veriti Thermal Cycler (Applied Biosystems, USA). Quantitative analysis of IL-6 and IFNB expression in THP1 dual reporter cells was performed by StepOne Real-Time PCR Systems (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... were denatured for 10 min at 70°C then reverse transcribed at 37°C for 90 min in a buffer containing Superscript II reverse transcriptase (Thermo Fisher Scientific), DTT (10 mM) ...
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bioRxiv - Biochemistry 2023Quote: ... The reaction mixtures were heated from 10°C to 70°C for approximately 1 hr by a QuantStudio 7 Pro Real-Time PCR System (Thermo Fisher Scientific). Approximately 770 evenly spaced measurements of fluorescence against the temperature gradient were recorded and processed by QuantStudio V1.3 (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were sliced into 20-μm sections using a cryostat (temperatures: sample holder −13°C, blade −12°C) (ThermoFisher CryoStar™ NX70) and mounted on superfrost slides (Fisher #1255015) ...
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bioRxiv - Plant Biology 2020Quote: ... bound to Protein G Dynabeads (Invitrogen) for 1.5h at 4°C and washed five times with SII buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... and protein G Dynabeads (10004D, Invitrogen).
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bioRxiv - Developmental Biology 2021Quote: ... Dynabeads Protein A (Thermo Fisher Scientific) was used for the immunoprecipitation ...
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bioRxiv - Cell Biology 2020Quote: ... or protein G Dynabeads (Fisher Scientific) for 1.5 h ...