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Citations for Thermo Fisher :
7451 - 7500 of 10000+ citations for C Reactive Protein Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... To estimate the molecular weight of the analyzed proteins PageRuler Prestained Protein Ladder (Thermo Fisher Scientific) was used.
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bioRxiv - Developmental Biology 2024Quote: ... Protein lysates were mixed with 20 μL Protein G-conjugated magnetic beads (#10009D, Thermo Fisher Scientific) preincubated with 1.0 μg antibody ...
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bioRxiv - Cancer Biology 2024Quote: ... Different protein fractions were collected using Subcellular Protein Fractionation Kit (Catalog No. 78840, Thermo Fisher Scientific) according to the manufacturer’s protocols ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentrations of tissue lysate supernatants were assessed by Pierce BCA Protein Assay Kit (Thermo Scientific). Samples were boiled with SDS-PAGE sample loading buffer (6X SDS ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were quantified using Pierce™ BCA Protein Assay kit (Cat. No. 23225, Thermo Fisher Scientific) and 500 µl of 2 mg/ml proteins were incubated with washed anti-FLAG magnetic beads (Cat ...
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bioRxiv - Cancer Biology 2024Quote: ... and protein concentrations were determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, 23225). The proteins were denatured by adding NuPAGE LDS Sample Buffer (4× ...
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bioRxiv - Cell Biology 2024Quote: ... protein concentrations in the lysates were quantified using Pierce BCA Protein Assay kit (Thermo Fisher Scientific). For cell-based assay ...
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bioRxiv - Neuroscience 2024Quote: ... and the supernatant protein concentration measured using BCA protein assay kit (Pierce, Thermo Fisher Scientific, USA). Equal amounts of proteins were separated on denaturing SDS-PAGE and blotted onto nitrocellulose membrane filters (Sartorius) ...
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bioRxiv - Plant Biology 2024Quote: ... and then the concentrations of dissolved proteins were measured using BCA protein assay (Thermo Fisher Scientific). Before trypsin digestion ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration was determined by using a Pierce™ BCA Protein Assay kit (Thermo Scientific™) and 20 μg protein was mixed with SDS-containing reducing sample buffer (Thermo Scientific™) ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration was measured using Pierce™ BCA Protein Assay Kit (Thermo Fisher, Waltham, MA, 23227). 30µg of protein homogenates were separated by SDS-PAGE on a 4-12% gradient Bis-Tris gel (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... The protein concentration was determined using PierceTM BCA protein assay kits (Cat#23225; Thermo Fisher Scientific). Brain extracts containing 60 ug of protein were loaded and separated on 6-10% denaturing polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Neuroscience 2024Quote: ... Total proteins amount was quantified by using Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific). For each sample ...
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bioRxiv - Microbiology 2024Quote: ... A 20μL aliquot of a 1:1 mixture of Protein A and Protein G Dynabeads (Invitrogen) was added and incubated for 4h at 4°C with rotation (50 rpm) ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentration in the extract was determined using a BCA protein assay kit (Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... protein concentration was measured using Pierce™ Bradford Protein Assay Kit (Thermo Fisher Scientific, USA, #23200), protein spectrum analysis was carried out using GENESYS™ 150 UV-Vis Spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... Protein content in the supernatant was quantified using the PierceTM BCA Protein Assay Kit (ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... and protein concentration was measured using the BCA method (Pierce BCA Protein Assay, Thermo Fisher, #23225).
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bioRxiv - Cancer Biology 2024Quote: ... and the concentration of isolated proteins was quantified using PierceTM BCA Protein Assay kit (Thermo Scientific). A total of 30µg of protein was used for analysis using SDS-PAGE ...
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bioRxiv - Cancer Biology 2024Quote: ... The total protein concentration was measured using Pierce BCA Protein Assay Kit (Thermo Fisher, Cat. 23227). Western blotting was performed as previously described (46) ...
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bioRxiv - Cell Biology 2024Quote: ... Soluble protein was quantified using the Pierce BCA Protein Assay Kit (Thermo Scientific, Bothell WA USA) with bovine serum albumin as the standard ...
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bioRxiv - Physiology 2024Quote: Protein content was quantified using the EZQ Protein Quantitation Kit (Molecular Probes, Thermo Fisher Scientific, #R33200). Then ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentration was determined using the BCA method (23227, Pierce BCA protein assay kit, Thermo Fisher). Immunoblotting was performed according to standard conditions ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentrations were quantified using a Micro BCA™ Protein Assay Kit (Thermo Scientific, Cat# 23235). Equivalent amounts of sample proteins in Laemmli buffer (With reducing agent ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein content in the lysates was quantified using the BCA Protein Assay (Thermo Fisher Scientific). To determine LAL activity in liver and spleen ...
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bioRxiv - Neuroscience 2024Quote: ... and total protein concentration was measured with the microBCA Protein Assay Kit (Thermo Fisher Scientific Inc.). 30 µl/well of capture antibody (anti-GCase antibody generated in in-house ...
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bioRxiv - Neuroscience 2024Quote: ... Protein content in the supernatant was quantified using Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2024Quote: ... Protein concentration was standardized using a bicinchoninic acid protein assay kit (Thermo Fisher Scientific, MA, USA). Each sample (10 μg of protein ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and then incubated in the dark for 1 h with secondary antibodies in 200 μl of blocking buffer (polyclonal goat anti-mouse Alexa Fluor® 488, 1:200 (A32723, ThermoFisher) and goat anti-rabbit Alexa Fluor® 647 ...
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bioRxiv - Immunology 2021Quote: ... Plates were blocked with blocking buffer made with 2% bovine serum albumin (BSA) Fraction V (Thermo Fisher Scientific, Waltham, MA, USA) and 1% gelatin from bovine skin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... Coverslips were again washed twice in blocking buffer for 10min and mounted on slides with ProLong Diamond Antifade (Thermo Fisher #P36965) for imaging ...
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bioRxiv - Cell Biology 2020Quote: ... and treated with donkey Alexa Fluor 488-conjugated anti-rabbit IgG secondary antibody (A21206, Life Technologies; 1:1000 in blocking buffer) overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... They then had further blocking solution added to them containing Alexa Fluor 488/568 secondary antibodies (1:1000; Thermo Fisher Scientific) in addition to DAPI (1:2000 ...
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bioRxiv - Neuroscience 2022Quote: ... slices were washed in TBS-T and incubated for 1 h at RT with a mixture of the following secondary antibodies diluted 1:200 in blocking solution: anti-guinea pig Alexa Fluor 488 (Life Technologies) and anti-rabbit Alexa Fluor 568 (Life Technologies ...
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bioRxiv - Biophysics 2022Quote: ... in blocking buffer followed by 10 min incubation at room temperature with goat anti-rabbit IgG/horseradish peroxidase-conjugate (1:10,000 dilution of 1 mg/mL stock in blocking buffer) (A16110, Thermo Fisher Scientific). The blot was developed using enhanced chemiluminescence detection reagents (Pierce™ ECL Western Blotting Substrate ...
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bioRxiv - Bioengineering 2022Quote: ... The following secondary antibodies were used at 1:1000 dilution in blocking solution: Alexa Fluor 647 anti-Rabbit (Thermo Fisher, #A21244), Alexa Fluor 647 anti-Goat (Thermo Fisher ...
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bioRxiv - Immunology 2022Quote: ... A secondary antibody mixture was made using the same permeabilization/blocking solution with 1:1000 dilution of anti-mouse Alexa Flour 488 (Thermo Fisher) overnight at 4 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... then incubated in blocking buffer composed of 1×PBS supplemented with 10% Gibco Fetal Bovine Serum (Thermo Fisher Scientific, Cat. A4766801) and 0.1% Triton X-100 ...
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bioRxiv - Neuroscience 2019Quote: To perform immunocytochemistry cells were fixated with 4% Paraformaldehyde and blocked with blocking buffer containing 5% Normal Goat Serum (Gibco®), 0,1% bovine serum albumin (SigmaAldrich ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were subsequently incubated for 1 hour at room temperature in blocking buffer containing the Alexa Fluor goat anti-mouse 488 or Alexa Fluor goat anti-rabbit 546 (Life Technologies) secondary antibodies (each at 1:1000 dilution ...
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bioRxiv - Cell Biology 2019Quote: ... After a final wash in blocking solution the coverslips were mounted on glass slides by inverting them onto mounting solution (ProLong Gold antifade, Molecular Probes). For the characterization of the HEK293 Flag-BirA*-LUZP1 line ...
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bioRxiv - Biophysics 2020Quote: ... cells were incubated in blocking buffer with 4 ng/µl of multiple-labeled goat anti-rabbit IgG secondary antibodies (Invitrogen, 31212) with different DOLs for the α-tubulin ...
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bioRxiv - Plant Biology 2020Quote: ... Slides were washed three times for 5 min in 1X PBS solution and then incubated for 3 h at room temperature in 100 μL blocking buffer containing Alexa 488-conjugated goat anti-rabbit secondary antibody (Molecular Probes, Invitrogen), diluted 1:200 in fresh blocking buffer ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibodies were diluted in 0.5% BSA in PBS blocking solution: 1:400 ZO-1 (Cat#40-2200, Thermo Fisher Scientific); 1:1000 MUC5AC (Cat#MA5-12178 ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were washed in TBS-T buffer and then incubated for an hour at room temperature with the corresponding horseradish peroxidase-conjugated preadsorbed secondary antibody (1/2000, blocking solution corresponding, Molecular Probes). Membranes were washed in TBS-T buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then rinsed again in buffer solution and then immersed in secondary antibody solution (blocking solution and 1:500 donkey anti-mouse IgG conjugated with Alexa Fluor 488 (Thermo Fisher Scientific Cat# A-21202 ...
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bioRxiv - Neuroscience 2020Quote: ... was added together with labelled α-bungarotoxin (to visualise post-synaptic acetylcholine receptors) in blocking solution for 1.5 h at room temperature (RT) (α-btx, Life Technologies). Then ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were then placed in blocking solution with secondary antibody [Alexa Fluor 790 Goat anti-Rabbit and/or Alexa Fluor 680 Goat anti-Mouse (Thermo Fisher)] at a dilution of 1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were washed in TBS-T buffer and then incubated for an hour at room temperature with the corresponding horseradish peroxidase-conjugated pre-adsorbed secondary antibody (1:5000, blocking solution corresponding, Molecular Probes). Membranes were washed in TBS-T buffer and immunoreactive bands were visualized with the SuperSignal West Pico Chemiluminescent Substrate (Pierce) ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated in blocking solution for 2 h at room temperature with fluorescent donkey anti-goat alexa fluor 488 (1:500, Invitrogen, A11055) and goat anti-rabbit alexa fluor 647 (1:500 ...