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Citations for Thermo Fisher :
7551 - 7600 of 10000+ citations for 3RS 4 Dimethylamino 3 methyl 2 2 diphenylbutanenitrile Isodidiavalo since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... and the residual bone marrow cells were plated in tissue-culture-treated microplates at a density of 2 × 105 cells per well in RPMI 1640 containing 2 mM glutamine (Thermo Fisher Scientific, 21875034), 10% FBS (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... Hydrogels with varying PEG-αMA wt% and DTT:MMP2-degradable crosslinker ratios (Table 1) were prepared with pH 8.0 N-2-hydroxyethylpiperaine-N-2-ethane sulfonic acid (HEPES; Thermo Fisher Scientific, Waltham, MA) as a solvent to allow base-catalyzed polymerization ...
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bioRxiv - Bioengineering 2022Quote: To assess endothelium barrier function we perfused microvessels with 2 μM Alexa Fluor647-conjugated 2 MDa dextran (Thermo Fisher Scientific, cat. no. D22914) in EGM-2 medium 48 h following inoculation with Bb or vehicle (BSK-II medium ...
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bioRxiv - Neuroscience 2022Quote: ... both proximal and distal chambers were washed with HibE imaging medium (Hibernate E [BrainBits] supplemented with 2% B-27, 2 mM GlutaMAX, 100 U/ml penicillin and 100 µg/ml streptomycin [Thermo Fisher Scientific/Gibco; 15140–122]) twice while maintaining fluidic isolation ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were loaded with 5 μM acetoxymethyl-ester of Fura-2 (Fura-2/AM) and 80 μg /ml pluronic F127 (Molecular Probes, Eugene, Oregon) for at least 30 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: ... and SARS-CoV-2 S protein were immobilized at 2 μg/ml on 96-well polystyrene microtiter plates Nunc MaxiSorp™ (Thermo Fisher Scientific). Following blocking with 1 × synthetic block (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... Peptides were concentrated and washed onto a trap column (75 μm × 2 cm, 2 μm C18 resin, 100 Å; Acclaim PepMap100, Thermo Fisher Scientific) for 5 min with 2% ACN and 0.05% aqueous TFA at a flow rate of 30 µl/min ...
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bioRxiv - Molecular Biology 2024Quote: ... Horseradish peroxidase (HRP)-conjugated secondary antibodies were from Dako (cat # P026002-2 and cat # P021702-2) and detected using either SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, cat # SKU:34579) or Western Lightning Plus-ECL Enhanced Chemiluminescence Substrate (Perkin Elmer ...
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bioRxiv - Developmental Biology 2024Quote: ... The pellet was then resuspended in 2 mL of MACS buffer (Hanks Buffered Salt Solution, 2% FBS, 2 mM EDTA) and incubated with streptavidin coated magnetic beads (Invitrogen MSNB-6002-74), spinning for 10 minutes at room temperature ...
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bioRxiv - Neuroscience 2024Quote: Experiments were conducted as described in our previous studies.(36) Neurons were loaded with the fluorescent calcium indicator Fura-2-acetomethoxyl ester (2 μg/mL, Molecular Probes, Eugene, OR) for 45 mins in the dark at room temperature and then allowed to de-esterify for 15 min at 37 °C in the warm external solution ...
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bioRxiv - Microbiology 2024Quote: ... and the resulting peptic peptides were collected on a trap column (2 mm × 2 cm) that was filled with POROS 20 R2 material (Thermo Fisher Scientific, USA) and kept at 0.5 °C ...
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bioRxiv - Neuroscience 2024Quote: ... The homogenate was diluted 1:1 with PBS with 2 x Protease/phosphatase inhibitors (2 tablets Thermo Scientific Pierce # A32961 per 10ml PBS). A portion of the resulting 10 % (w/v ...
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bioRxiv - Cell Biology 2024Quote: ... placed into individual wells in a 12 well suspension culture plate (greiner bio-one Cellstar, #665 102) containing warmed, oxygenated resting media (2 ml, RPMI 1640 with 2%FBS (GIBCOTM ThermoFisher Scientific, Cat# 11875093) and incubated overnight while on an orbital shaker.
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bioRxiv - Cancer Biology 2024Quote: ... Secondary antibodies (AlexaFluor546 anti-mouse, ThermoFisher, cat: A-11030, at 2 µg/mL; AlexaFluor647 anti-rabbit, ThermoFisher, cat: A32733, at 2 µg/mL) were incubated 2h/shaking at RT (BSA at 2% ...
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bioRxiv - Evolutionary Biology 2024Quote: ... then incubated at 37C for 5 min in 2 ml M3+BPYE media containing 1 ml Turbo DNase (2 U/μL) (ThermoFisher cat. no. AM2239) per 36 ml ...
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bioRxiv - Developmental Biology 2024Quote: ... lyz:DsRed2nz50double transgenic fish (2 males and 2 females) were removed with forceps and placed in dissociation media (1:1 DMEM:Liberase) (Gibco A1896701/ Sigma-Aldrich 05401119001). The tissue was slowly pipetted up and down at room temperature for 45 minutes with a 1000 µL pipette set to 500 µL to ensure gentle dissociation ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary neurospheres grown for 3 days (3-4 million cells) were harvested and crosslinked with 0.5% formaldehyde (Thermo Fisher, cat. no. 28908) in 0.1 M DPBS for 5 min at RT with soft agitation ...
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bioRxiv - Genetics 2022Quote: ... and washed 3-4 times with fresh 10% RNA later solution (Thermo Fisher Scientific). After the last wash ...
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bioRxiv - Zoology 2022Quote: ... The excised tissues were then placed (3-4 pieces) on gelatin (Attachment Factor, ThermoFisher)-coated dish (35 mm ...
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bioRxiv - Molecular Biology 2021Quote: ... 3-4 μl of samples were applied onto grids using a Vitrobot (Thermo Fisher). Grids were blotted with “595” filter papers (Ted Pella ...
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bioRxiv - Genetics 2021Quote: Calu-3 cells were mock transfected with 4 μL of lipofectamine 3000 (ThermoFisher Scientific) in Opti-MEM (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... with 10% FBS and split every 3-4 days using TrypLE Express (Thermo Scientific). Low-passage ...
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bioRxiv - Microbiology 2023Quote: ... sonicated via a Sonic Dismembrator 100 (Fisher Scientific, setting 3, 15 s, 4 pulses), and incubated on ice for 1 hr after the addition of 2 μL benzonase (EMD Millipore) ...
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bioRxiv - Molecular Biology 2024Quote: ... The medium was replaced after 3-4 hr with neurobasal medium (Gibco, Cat# 21103049) supplemented with 2% v/v B27 (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... cells were washed 3 times with DMEM and fixed with 4% paraformaldehyde (Thermo Fisher) at room temperature (RT ...
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bioRxiv - Cancer Biology 2024Quote: ... and resolved on 3-8% or 4-12% gradient SDS-PAGE gels (Invitrogen, NuPAGE). After transfer to a polyvinylidene difluoride (PVDF ...
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bioRxiv - Immunology 2024Quote: ... at a ratio of 4:3:1 using Lipofectamine 3000 kit (Thermo Fisher Scientific). Viral supernatant was filtered ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were passaged every 3-4 days using 0.25% Trypsin-EDTA (Fisher Scientific) incubated at 37°C for 3-5 minutes to detach cells ...
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bioRxiv - Neuroscience 2024Quote: ... for 3 h at 4°C and then with protein G magnetic beads (ThermoFisher) at 4°C for an additional 2 h to obtain immunoprecipitated RNA fragments ...
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bioRxiv - Genomics 2024Quote: ... were treated 3-4 times with 50 U of T4 PNK (Thermo Fisher Scientific) and 100 U of λ-exonuclease (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... antibodies overnight at 4°C and then incubated for an additional 3 hours at 4°C with 50 μL DynabeadsTM protein G (Life Technologies, 10004D). Following three washes with 1% Triton-TBS lysis buffer ...
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bioRxiv - Developmental Biology 2022Quote: ... Human naive PSCs were passaged as single cells every 4 days at split ratio 1:3 or 1:4 following dissociation with TrypLE (Gibco 12563-029) for 10 minutes (min ...
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bioRxiv - Developmental Biology 2023Quote: ... Human naive PSCs were passaged as single cells every 4 days at split ratio 1:3 or 1:4 following dissociation with TrypLE (Gibco 12563-029) for 10 minutes at room temperature (RT) ...
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bioRxiv - Biophysics 2021Quote: ... or a combination of pepsin and fungal protease type XIII (manually conjugated to POROS™ 20 AL Aldehyde Activated Resin, Thermo Fisher Scientific, and packed in a 2 mm ID x 2 cm IDEX C-130B column, Fisher Scientific, Hampton, NH), loaded on a C18 analytical column (Hypersil Gold ...
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bioRxiv - Biochemistry 2020Quote: ... Single colonies of strains were inoculated into 2 mL of minimal medium containing 2% succinate and 50 µg/mL kanamycin in 14 mL polypropylene culture tubes (Fisher Scientific, Waltham, MA, USA), then grown to mid-exponential growth phase with shaking at 200 rpm and 30 °C on an Innova 2300 platform shaker ...
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bioRxiv - Microbiology 2021Quote: ... The cell monolayers were then blocked with blocking buffer (2% Blotting-Grade Blocker [Bio-Rad] supplemented with 2% goat serum [Gibco] in PBS-Tween) for 1 hr ...
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bioRxiv - Immunology 2022Quote: ... iAT2s were serially passaged approximately every 2 weeks by dissociation into single cells via the sequential application of dispase (2 mg/mL; Thermo Fisher Scientific; 17105-04) and 0.05% trypsin (Invitrogen ...
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bioRxiv - Developmental Biology 2023Quote: ... the corneas were cut into 2×2 mm pieces and sterilized by soaking in 1X Gibco phosphate buffered saline (PBS) (Fisher Scientific, 10-010-023) containing 1% HyClone penicillin streptomycin 100X solution (Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... Restriction-digested shuttle vectors -1-6 (33) and -2-1 (14) for wc-1 and wc-2 respectively were recombined with PCR (Thermo Fisher Scientific, Catalog # F549S) products amplified with primers bearing point mutations of wc-1 or wc-2 ...
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bioRxiv - Plant Biology 2022Quote: ... total soluble proteins were extracted from 50 mg of plant materials grown as indicated previously with 100 μL 2 x Laemmli buffer supplemented with 1% Phosphatase Inhibitor Cocktail 2 (Thermo Fisher Scientific Inc., 78430). Proteins were denatured for 10 min at 95°C and separated on 10% or 8% SDS-PAGE ...
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bioRxiv - Genomics 2022Quote: ... samples were incubated with primary Chr 2 intron probes for 30 hrs at 37°C in 50% Hybridization Buffer (2×SSC, 50% Formamide (v/v) (Thermo Fisher Scientific, Cat. AM9344), 10%(w/v ...
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bioRxiv - Immunology 2024Quote: ... species-matched allogeneic T cells were added to passaged human organoids or passaged Balb/c small intestinal organoids with either human IL-2 (30 U/mL, Peprotec) and Dynabeads human T-activator CD3/CD28 (ThermoFisher, 2 µL per well), or murine IL-2 (30 U/mL ...
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bioRxiv - Cell Biology 2024Quote: ... 50 or 100 Multiplicity of Infection (MOIs) as described in the individual experiments in 2% complete medium (2%CM - DMEM F12 (Thermo Fisher, Waltham, MA, USA), 2% Fetal Bovine Serum (FBS ...
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bioRxiv - Bioengineering 2024Quote: ... PCs and ACs were stained with neural/glial antigen 2 (NG-2) antibody (eBioscience, 14-6504-80, 1:100) and glial fibrillary acidic protein (GFAP) antibody (Invitrogen, PA1-10019, 1:1000), respectively ...
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bioRxiv - Genetics 2024Quote: ... 2 µL of a 1:100 dilution of ERCC ExFold RNA Spike-In Mix 1 or Mix 2 (Thermo Fisher Scientific, #44-567-39) were added to 1 µg of total RNA ...
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bioRxiv - Cell Biology 2021Quote: ... cells were transfected with the respective pcDNA3 constructs and subsequently selected with 2 mg/ml G418 (Geneticin from Thermo Fisher Scientific; 3 mg/ml for 10 days). Surviving cells were expanded in culture medium containing G418.
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bioRxiv - Genetics 2024Quote: ... Slides were then washed for 2 minutes in PBS 3 times then incubated for 2 hours with goat anti–rabbit IgG/Alexa Fluor 594 secondary antibody (Thermo Fisher Scientific A-11012 at 1:1000) in blocking buffer at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: A concentration of 6 × 103 cells was loaded with 5 mM 4-amino-5-methylamino-2’,7’- difluorofluorescein diacetate (DAF-FM, Molecular Probes, Thermo Fisher, Sao Paulo, Brazil) after 72 h of treatment ...
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bioRxiv - Cell Biology 2020Quote: ... Tetramethylrhodamine methyl ester (TMRM, cat n. T668, Molecular Probes, Leiden, The Netherlands) was dissolved in DMSO to obtain a 10 mM stock solution and then diluted in the appropriate buffer ...
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bioRxiv - Cell Biology 2020Quote: ... cells were co-stained with 16.7 nM tetramethylrhodamine methyl ester (TMRM; Invitrogen) and 100 nM Mitotracker Green (MTG ...