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Citations for Thermo Fisher :
7501 - 7550 of 10000+ citations for 3RS 4 Dimethylamino 3 methyl 2 2 diphenylbutanenitrile Isodidiavalo since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... 2% B-27 with vitamin A (#17504044, Thermo Fisher), 2 mM GlutaMAX (#11500626 ...
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bioRxiv - Cancer Biology 2024Quote: ... diluted 1:2 in 1X PBS-/- (Gibco, 14190-144), and stored at −80°C ...
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bioRxiv - Cancer Biology 2024Quote: ... using an iBlot 2 Gel Transfer Device (Invitrogen #IB21001). Membranes were then washed in 25 mL 1X TBS (50mM Tris-Cl ...
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bioRxiv - Cell Biology 2024Quote: ... containing 2% B27 (0080085-SA; Invitrogen, Carlsbad, CA, USA), 1% N2 (17502-048 ...
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bioRxiv - Cell Biology 2024Quote: ... and 0.1 mM 2-Mercaptoethanol (Thermo Fisher Scientific, M3148). Afterwards ...
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bioRxiv - Neuroscience 2024Quote: ... 2 mM L-glutamine (25030-081, Thermo Fisher Scientific), 1× MEM Non-Essential Amino Acid (11140-050 ...
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bioRxiv - Genetics 2024Quote: ... 2 μl of 25 mM MgCl2 (Applied Biosystems, USA), 0.8 μl of 20 mg/ml BSA (BioLabs ...
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bioRxiv - Cell Biology 2024Quote: ... 2 µL PAGEruler Plus Prestained Protein Ladder (Thermo Scientific) was also loaded onto each gel ...
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bioRxiv - Cell Biology 2024Quote: Caco-2 cells were maintained in DMEM GlutaMax (Gibco), supplemented with 20% (v/v ...
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bioRxiv - Genetics 2024Quote: ... and 2 mM of Hoechst 33342 (Thermo Fisher Scientific) to label mosquito hemocytes ...
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bioRxiv - Developmental Biology 2024Quote: ... cells were incubated with 2-NBDG (200μM; N13195, Invitrogen) for 15 minutes at 37°C ...
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bioRxiv - Evolutionary Biology 2024Quote: ... or SM/5 plates (2 g glucose (Fisher Scientific), 2 g BactoPeptone (Oxoid) ...
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bioRxiv - Developmental Biology 2024Quote: ... 2 mL of TrypLE™ Express Enzyme (Gibco, 12604013) were added to each well ...
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bioRxiv - Developmental Biology 2024Quote: ... 2 mL of BGJb media ((Thermo Fisher Scientific, 12591038), 1% Antibiotic-Antimycotic (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... 1:200 [v/v] N-2 supplement (Gibco, 7502048), 1:50 [v/v] B-27 supplement minus vitamin A (Gibco ...
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bioRxiv - Developmental Biology 2024Quote: ... 2% Glutamax and 15 μM HEPES (Gibco 15630-049) supplemented with 10ng/ml Epiregulin (Peprotech 100-04) ...
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bioRxiv - Cancer Biology 2021Quote: ... incubated with 200 nM tetramethylrhodamine methyl ester (TMRM) (Invitrogen, Milan, Italy) for 10 minutes at 37°C and immediately measured on a FacsCalibur flow cytometer (Becton Dickinson ...
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bioRxiv - Plant Biology 2023Quote: ... and Methyl-PEG-Maleimide Reagent (MM(PEG)24) (Thermo Fisher, 22713). NEM was added to Chlamydomonas cultures growing as indicated in each case to a final concentration of 10 mM ...
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bioRxiv - Cell Biology 2023Quote: Mitochondrial membrane potential was determined using tetramethylrhodamine methyl ester (TMRM) (Invitrogen). Cells were plated in 12-well plates and transfected with the indicated siRNAs for the indicated times ...
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bioRxiv - Developmental Biology 2022Quote: ... 80□μL of N-methyl-N-(trimethylsilyl) trifluoroacetamide□(Thermo Fisher Scientific) and 70□μL ethyl acetate (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... MitoProbe TMRM (tetramethylrhodamine methyl ester) kit for flow cytometry (Invitrogen, # M20036) was used as per manufacturer’s instructions to measure the membrane potential ...
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bioRxiv - Neuroscience 2024Quote: ... and Paraquat (#227320050: Methyl viologen hydrate 98%) was from Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were incubated with 100 nM Tetramethylrhodamine methyl ester (TMRM, Thermofisher) and 1 μg/ml Hoechst 33342 (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... Media was changed daily and the cells passaged every 3–4 days at a ratio of 1:4 using the StemPro EZPassage tool (ThermoFisher Scientific).
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bioRxiv - Cell Biology 2024Quote: ... hansenii cells were concentrated 10x in 200 μL YPD containing 40 μM FM4-64 dye (N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide) dye (Invitrogen™) to label endosomes for 8 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... and washed for 3 min on a C18 reverse-phase precolumn (3 μm particle size, 100 Å pore size, 75 μm inner diameter, 2 cm in length, from Thermo Fisher Scientific, Waltham, MA, USA). Peptides were separated on an Aurora C18 reverse-phase resin (1.6 μm particle size ...
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bioRxiv - Cell Biology 2022Quote: Transient genetic silencing of HOIP and FLOT1/2 was performed by reverse transfection of HT-29 cells with the following siRNAs: human siHOIP (RNF31) (Silencer® Select siRNA #1 s30108, #2 s30109, #3 s30110, 40 nM) (Thermo Fisher Scientific, Waltham, MA, USA), human FLOT1 (ON-TARGETplus Human FLOT1 siRNA SMART POOL ...
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bioRxiv - Cancer Biology 2024Quote: ... an additional 150 µL of a 1:1 mixture of NK-Xpander complete medium:OncoPro complete medium containing 500 U/mL rhIL-2 and 10 µM Invitrogen™ CellEvent™ Caspase-3/7 Red Detection Reagent (Thermo Fisher Scientific, cat# C10430) was added to each well ...
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bioRxiv - Cell Biology 2024Quote: ... slides were washed in PBS 3×5 minutes and then stained with the secondary antibody Goat anti-rabbit AlexaFluor555 (Invitrogen #A21429, working concentration 2 µg/µl) or Goat anti-rat Alexa488 (Thermofisher #A-11006 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells nuclei were counter-stained using 4′,6-Diamidine-2′-phenylindole dihydrochloride (DAPI) mounting medium (ProLong™ Diamond Antifade Mountant with DAPI, Thermo Fisher Scientific, Warsaw, Poland). Preparations were observed using the confocal microscope under magnification 630× and processed with Fiji software (ImageJ 1.52n ...
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bioRxiv - Neuroscience 2021Quote: ... sections were rinsed and mounted immediately after onto glass slides coated with gelatin in Fluoromont-G with 4’,6-diamidino-2-phenylindole (DAPI) (00-4959-52, Invitrogen, Thermo Fisher Scientific, MA, USA) as counterstaining.
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bioRxiv - Molecular Biology 2022Quote: A concentration of 6 × 103 cells was loaded with 5 mM 4-amino-5-methylamino-2’,7’- difluorofluorescein diacetate (DAF-FM, Molecular Probes, Thermo Fisher, Sao Paulo, Brazil) after 72 h of treatment ...
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bioRxiv - Bioengineering 2022Quote: ... pulsing 2 s on and 4 s off for a total of 10 min of “on” time (Fisher Scientific, catalog no. FB120A220, probe CL-18). Note that from harvesting onward the libraries were kept at 4 °C ...
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bioRxiv - Bioengineering 2024Quote: ... Tissue sections were stained with hematoxylin and eosin (H&E) or mounted with 4′,6-diamidino-2-phenylindole (DAPI) Fluoromount-G (Thermo Fisher Scientific, Waltham, MA, USA). They were imaged with SLIDEVIEW VS200 Slide Scanner (Olympus ...
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bioRxiv - Cancer Biology 2024Quote: ... the tissues were stained with DAPI (4′,6-diamidino-2-phenylindole) for 5 minutes and mounted in ProLong™ Diamond Antifade Mountant (Thermo Fisher Scientific, MA, USA).
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bioRxiv - Developmental Biology 2020Quote: Islet cell clusters were incubated (25 min at 37 °C) in RPMI supplemented with 2 μM Fura-2 acetoxymethyl ester (Molecular Probes, Eugene, OR). Fluorescence imaging was performed using a Nikon Eclipse TE2000-U microscope equipped with an epifluorescence illuminator (SUTTER ...
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bioRxiv - Pathology 2020Quote: ... or Abberior STAR635P (Abberior, 2-0142-007-2, dilution 1:50) and donkey anti-rabbit conjugated to Alexa-555 (Thermo Fisher, A-31572) or Cy3 (Jackson Immuno Research ...
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bioRxiv - Microbiology 2021Quote: ... cells in each well were infected with 2000 TCID50 SARS-CoV-2 pseudovirus in 100 µL of culture medium containing 2% FBS (Gibco, Cat. No. 10099141C). Culture medium was refreshed every 12 hours ...
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bioRxiv - Immunology 2022Quote: ... and transferred using electrophoresis to nitrocellulose membranes by using the iBlot 2 Gel Transfer Device (Thermo Scientific, iBlot 2 NC Regular Stacks, IB23001, Invitrogen, Walham, MA). Membranes were blocked for 1 h at RT in 5% (w/v ...
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bioRxiv - Microbiology 2022Quote: Primary human CD4+ T cells were incubated with 20U/ml interleukin-2 (IL-2) alone or in combination with α-CD3/CD28 antibody-coated beads (100 μL/30 x 106 cells) (Gibco/Thermo Fisher) for 72 hours prior to infection with 100ng of p24 equivalent of pseudotyped pMorpheus-V5 ...
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bioRxiv - Microbiology 2022Quote: SARS-CoV-2 N and goblet cells were co-stained using rabbit anti-SARS-CoV-2 Nucleocapsid monoclonal antibody (HL453) (Invitrogen, cat# MA5-36272) (1:100 dilution ...
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bioRxiv - Immunology 2021Quote: ... 200μL of platelet suspension (2×106 platelets in HBSSCa-Mg-) from healthy donors was labelled with calcein-AM (2 μg/mL final concentration, ThermoFisher Scientific Cat # C3100MP) for 15 mins and then R406 ...
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bioRxiv - Neuroscience 2022Quote: ... with 16 μM BSA in the presence of CaCl2 (1.3mM) and fura-2-acetoxymethyl ester (fura 2-AM, 5 μM: Molecular Probes, Eugene, OR, USA), and they were incubated at 37 °C for 25 min ...
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bioRxiv - Immunology 2020Quote: ... Purified monoclonal antibody stock at a concentration of approximately 1mg/mL was 1:2 serially diluted in infection media (DMEM with 2% FBS and 1X penicillin-streptomycin (Thermo Fisher catalog 15140122) starting at 1:20 and ending at a dilution of 1:20,480 ...
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bioRxiv - Microbiology 2023Quote: Vero E6 cells were transfected with 400 ng/well 2-E plasmids or 2-E mutations using Lipofectamine 3000 Transfection Reagent (L3000015, Thermo Fisher, MA, USA). The control group was Vero E6 cells transfected with 400 ng/well pcDNA3.1 vector plasmids alone ...
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bioRxiv - Developmental Biology 2023Quote: ... we washed the mature COCs in synthetic oviductal fluid medium (SOF) containing N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid (HEPES-TL, Thermofisher Scientific, Waltham, MA) and SOF for fertilization (SOF-FERT ...
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bioRxiv - Plant Biology 2022Quote: ... Each sample was incubated with 50 µm of 2’,7’-Bis-(2- carboxyethyl)-5-(6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM; Molecular Probes, Eugene, OR) at 28°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Wells were coated with 1 μg Gfp for anti-GfpNB or 1 μg commercially available SARS-CoV-2 Spike-RBD-domain protein for SARS-CoV-2 nanobody-Cts1-fusion proteins (Invitrogen, Waltham Massachusetts, USA). Recombinant Gfp was produced in E ...
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bioRxiv - Molecular Biology 2022Quote: ... Dm III-2 and Dm III-2 + cys production was confirmed via western blot using 6x His-tag Ab (Invitrogen, Carlsbad, CA, USA). Positive clones were expanded ...
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bioRxiv - Cancer Biology 2024Quote: ... Secondary antibodies (AlexaFluor546 anti-mouse, ThermoFisher, cat: A-11030, at 2 µg/mL; AlexaFluor647 anti-rabbit, ThermoFisher, cat: A32733, at 2 µg/mL) were incubated 2h/shaking at RT (BSA at 2% ...